A simplified CsCL protocol for lambda DNA purification: no enzymatic treatment/one phenol extraction
Autor(a) principal: | |
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Data de Publicação: | 2000 |
Outros Autores: | |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Genetics and Molecular Biology |
Texto Completo: | http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572000000100011 |
Resumo: | A modification of the CsCl gradient centrifugation method for DNA phage purification is presented. It avoids the enzymatic steps as well the need for a preliminary phage titration, a tedious process proposed in the majority of the protocols in use. The quality of the DNA obtained makes it amenable for additional manipulations like digestions, ligations, labelling, subcloning, etc. |
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Genetics and Molecular Biology |
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|
spelling |
A simplified CsCL protocol for lambda DNA purification: no enzymatic treatment/one phenol extractionA modification of the CsCl gradient centrifugation method for DNA phage purification is presented. It avoids the enzymatic steps as well the need for a preliminary phage titration, a tedious process proposed in the majority of the protocols in use. The quality of the DNA obtained makes it amenable for additional manipulations like digestions, ligations, labelling, subcloning, etc.Sociedade Brasileira de Genética2000-03-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersiontext/htmlhttp://old.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572000000100011Genetics and Molecular Biology v.23 n.1 2000reponame:Genetics and Molecular Biologyinstname:Sociedade Brasileira de Genética (SBG)instacron:SBG10.1590/S1415-47572000000100011info:eu-repo/semantics/openAccessSantelli,Roberto V.Navarro-Cattapan,Luci Deiseeng2000-08-25T00:00:00Zoai:scielo:S1415-47572000000100011Revistahttp://www.gmb.org.br/ONGhttps://old.scielo.br/oai/scielo-oai.php||editor@gmb.org.br1678-46851415-4757opendoar:2000-08-25T00:00Genetics and Molecular Biology - Sociedade Brasileira de Genética (SBG)false |
dc.title.none.fl_str_mv |
A simplified CsCL protocol for lambda DNA purification: no enzymatic treatment/one phenol extraction |
title |
A simplified CsCL protocol for lambda DNA purification: no enzymatic treatment/one phenol extraction |
spellingShingle |
A simplified CsCL protocol for lambda DNA purification: no enzymatic treatment/one phenol extraction Santelli,Roberto V. |
title_short |
A simplified CsCL protocol for lambda DNA purification: no enzymatic treatment/one phenol extraction |
title_full |
A simplified CsCL protocol for lambda DNA purification: no enzymatic treatment/one phenol extraction |
title_fullStr |
A simplified CsCL protocol for lambda DNA purification: no enzymatic treatment/one phenol extraction |
title_full_unstemmed |
A simplified CsCL protocol for lambda DNA purification: no enzymatic treatment/one phenol extraction |
title_sort |
A simplified CsCL protocol for lambda DNA purification: no enzymatic treatment/one phenol extraction |
author |
Santelli,Roberto V. |
author_facet |
Santelli,Roberto V. Navarro-Cattapan,Luci Deise |
author_role |
author |
author2 |
Navarro-Cattapan,Luci Deise |
author2_role |
author |
dc.contributor.author.fl_str_mv |
Santelli,Roberto V. Navarro-Cattapan,Luci Deise |
description |
A modification of the CsCl gradient centrifugation method for DNA phage purification is presented. It avoids the enzymatic steps as well the need for a preliminary phage titration, a tedious process proposed in the majority of the protocols in use. The quality of the DNA obtained makes it amenable for additional manipulations like digestions, ligations, labelling, subcloning, etc. |
publishDate |
2000 |
dc.date.none.fl_str_mv |
2000-03-01 |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572000000100011 |
url |
http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572000000100011 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
10.1590/S1415-47572000000100011 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
text/html |
dc.publisher.none.fl_str_mv |
Sociedade Brasileira de Genética |
publisher.none.fl_str_mv |
Sociedade Brasileira de Genética |
dc.source.none.fl_str_mv |
Genetics and Molecular Biology v.23 n.1 2000 reponame:Genetics and Molecular Biology instname:Sociedade Brasileira de Genética (SBG) instacron:SBG |
instname_str |
Sociedade Brasileira de Genética (SBG) |
instacron_str |
SBG |
institution |
SBG |
reponame_str |
Genetics and Molecular Biology |
collection |
Genetics and Molecular Biology |
repository.name.fl_str_mv |
Genetics and Molecular Biology - Sociedade Brasileira de Genética (SBG) |
repository.mail.fl_str_mv |
||editor@gmb.org.br |
_version_ |
1752122377449242624 |