Altered expression of the caffeine synthase gene in a naturally caffeine-free mutant of Coffea arabica

Detalhes bibliográficos
Autor(a) principal: Maluf,Mirian Perez
Data de Publicação: 2009
Outros Autores: Silva,Carla Cristina da, Oliveira,Michelle de Paula Abreu de, Tavares,Aline Gomes, Silvarolla,Maria Bernadete, Guerreiro Filho,Oliveiro
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Genetics and Molecular Biology
Texto Completo: http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572009000400023
Resumo: In this work, we studied the biosynthesis of caffeine by examining the expression of genes involved in this biosynthetic pathway in coffee fruits containing normal or low levels of this substance. The amplification of gene-specific transcripts during fruit development revealed that low-caffeine fruits had a lower expression of the theobromine synthase and caffeine synthase genes and also contained an extra transcript of the caffeine synthase gene. This extra transcript contained only part of exon 1 and all of exon 3. The sequence of the mutant caffeine synthase gene revealed the substitution of isoleucine for valine in the enzyme active site that probably interfered with enzymatic activity. These findings indicate that the absence of caffeine in these mutants probably resulted from a combination of transcriptional regulation and the presence of mutations in the caffeine synthase amino acid sequence.
id SBG-1_498781e54acd94426fc908d979432130
oai_identifier_str oai:scielo:S1415-47572009000400023
network_acronym_str SBG-1
network_name_str Genetics and Molecular Biology
repository_id_str
spelling Altered expression of the caffeine synthase gene in a naturally caffeine-free mutant of Coffea arabicacoffeecup qualitydifferential expressionnaturally decaffeinatedIn this work, we studied the biosynthesis of caffeine by examining the expression of genes involved in this biosynthetic pathway in coffee fruits containing normal or low levels of this substance. The amplification of gene-specific transcripts during fruit development revealed that low-caffeine fruits had a lower expression of the theobromine synthase and caffeine synthase genes and also contained an extra transcript of the caffeine synthase gene. This extra transcript contained only part of exon 1 and all of exon 3. The sequence of the mutant caffeine synthase gene revealed the substitution of isoleucine for valine in the enzyme active site that probably interfered with enzymatic activity. These findings indicate that the absence of caffeine in these mutants probably resulted from a combination of transcriptional regulation and the presence of mutations in the caffeine synthase amino acid sequence.Sociedade Brasileira de Genética2009-01-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersiontext/htmlhttp://old.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572009000400023Genetics and Molecular Biology v.32 n.4 2009reponame:Genetics and Molecular Biologyinstname:Sociedade Brasileira de Genética (SBG)instacron:SBG10.1590/S1415-47572009005000090info:eu-repo/semantics/openAccessMaluf,Mirian PerezSilva,Carla Cristina daOliveira,Michelle de Paula Abreu deTavares,Aline GomesSilvarolla,Maria BernadeteGuerreiro Filho,Oliveiroeng2009-11-17T00:00:00Zoai:scielo:S1415-47572009000400023Revistahttp://www.gmb.org.br/ONGhttps://old.scielo.br/oai/scielo-oai.php||editor@gmb.org.br1678-46851415-4757opendoar:2009-11-17T00:00Genetics and Molecular Biology - Sociedade Brasileira de Genética (SBG)false
dc.title.none.fl_str_mv Altered expression of the caffeine synthase gene in a naturally caffeine-free mutant of Coffea arabica
title Altered expression of the caffeine synthase gene in a naturally caffeine-free mutant of Coffea arabica
spellingShingle Altered expression of the caffeine synthase gene in a naturally caffeine-free mutant of Coffea arabica
Maluf,Mirian Perez
coffee
cup quality
differential expression
naturally decaffeinated
title_short Altered expression of the caffeine synthase gene in a naturally caffeine-free mutant of Coffea arabica
title_full Altered expression of the caffeine synthase gene in a naturally caffeine-free mutant of Coffea arabica
title_fullStr Altered expression of the caffeine synthase gene in a naturally caffeine-free mutant of Coffea arabica
title_full_unstemmed Altered expression of the caffeine synthase gene in a naturally caffeine-free mutant of Coffea arabica
title_sort Altered expression of the caffeine synthase gene in a naturally caffeine-free mutant of Coffea arabica
author Maluf,Mirian Perez
author_facet Maluf,Mirian Perez
Silva,Carla Cristina da
Oliveira,Michelle de Paula Abreu de
Tavares,Aline Gomes
Silvarolla,Maria Bernadete
Guerreiro Filho,Oliveiro
author_role author
author2 Silva,Carla Cristina da
Oliveira,Michelle de Paula Abreu de
Tavares,Aline Gomes
Silvarolla,Maria Bernadete
Guerreiro Filho,Oliveiro
author2_role author
author
author
author
author
dc.contributor.author.fl_str_mv Maluf,Mirian Perez
Silva,Carla Cristina da
Oliveira,Michelle de Paula Abreu de
Tavares,Aline Gomes
Silvarolla,Maria Bernadete
Guerreiro Filho,Oliveiro
dc.subject.por.fl_str_mv coffee
cup quality
differential expression
naturally decaffeinated
topic coffee
cup quality
differential expression
naturally decaffeinated
description In this work, we studied the biosynthesis of caffeine by examining the expression of genes involved in this biosynthetic pathway in coffee fruits containing normal or low levels of this substance. The amplification of gene-specific transcripts during fruit development revealed that low-caffeine fruits had a lower expression of the theobromine synthase and caffeine synthase genes and also contained an extra transcript of the caffeine synthase gene. This extra transcript contained only part of exon 1 and all of exon 3. The sequence of the mutant caffeine synthase gene revealed the substitution of isoleucine for valine in the enzyme active site that probably interfered with enzymatic activity. These findings indicate that the absence of caffeine in these mutants probably resulted from a combination of transcriptional regulation and the presence of mutations in the caffeine synthase amino acid sequence.
publishDate 2009
dc.date.none.fl_str_mv 2009-01-01
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572009000400023
url http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572009000400023
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv 10.1590/S1415-47572009005000090
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv text/html
dc.publisher.none.fl_str_mv Sociedade Brasileira de Genética
publisher.none.fl_str_mv Sociedade Brasileira de Genética
dc.source.none.fl_str_mv Genetics and Molecular Biology v.32 n.4 2009
reponame:Genetics and Molecular Biology
instname:Sociedade Brasileira de Genética (SBG)
instacron:SBG
instname_str Sociedade Brasileira de Genética (SBG)
instacron_str SBG
institution SBG
reponame_str Genetics and Molecular Biology
collection Genetics and Molecular Biology
repository.name.fl_str_mv Genetics and Molecular Biology - Sociedade Brasileira de Genética (SBG)
repository.mail.fl_str_mv ||editor@gmb.org.br
_version_ 1752122382383841280

Registros relacionados