A two-step strategy for the complementation of M: tuberculosis mutants
Autor(a) principal: | |
---|---|
Data de Publicação: | 2011 |
Outros Autores: | , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Genetics and Molecular Biology |
Texto Completo: | http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572011000200020 |
Resumo: | The sequence of Mycobacterium tuberculosis, completed in 1998, facilitated both the development of genomic tools, and the creation of a number of mycobacterial mutants. These mutants have a wide range of phenotypes, from attenuated to hypervirulent strains. These phenotypes must be confirmed, to rule out possible secondary mutations that may arise during the generation of mutant strains. This may occur during the amplification of target genes or during the generation of the mutation, thus constructing a complementation strain, which expresses the wild-type copy of the gene in the mutant strain, becomes necessary. In this study we have introduced a two-step strategy to construct complementation strains using the Ag85 promoter. We have constitutively expressed dosR and have shown dosR expression is restored to wild-type level. |
id |
SBG-1_9c82fd00a4614f97ada38ff689ab8687 |
---|---|
oai_identifier_str |
oai:scielo:S1415-47572011000200020 |
network_acronym_str |
SBG-1 |
network_name_str |
Genetics and Molecular Biology |
repository_id_str |
|
spelling |
A two-step strategy for the complementation of M: tuberculosis mutantstuberculosismutagenesisconstitutive expressioncomplementationThe sequence of Mycobacterium tuberculosis, completed in 1998, facilitated both the development of genomic tools, and the creation of a number of mycobacterial mutants. These mutants have a wide range of phenotypes, from attenuated to hypervirulent strains. These phenotypes must be confirmed, to rule out possible secondary mutations that may arise during the generation of mutant strains. This may occur during the amplification of target genes or during the generation of the mutation, thus constructing a complementation strain, which expresses the wild-type copy of the gene in the mutant strain, becomes necessary. In this study we have introduced a two-step strategy to construct complementation strains using the Ag85 promoter. We have constitutively expressed dosR and have shown dosR expression is restored to wild-type level.Sociedade Brasileira de Genética2011-01-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersiontext/htmlhttp://old.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572011000200020Genetics and Molecular Biology v.34 n.2 2011reponame:Genetics and Molecular Biologyinstname:Sociedade Brasileira de Genética (SBG)instacron:SBG10.1590/S1415-47572011000200020info:eu-repo/semantics/openAccessMovahedzadeh,FarahnazFrita,RosangelaGutka,Hiten J.eng2011-06-02T00:00:00Zoai:scielo:S1415-47572011000200020Revistahttp://www.gmb.org.br/ONGhttps://old.scielo.br/oai/scielo-oai.php||editor@gmb.org.br1678-46851415-4757opendoar:2011-06-02T00:00Genetics and Molecular Biology - Sociedade Brasileira de Genética (SBG)false |
dc.title.none.fl_str_mv |
A two-step strategy for the complementation of M: tuberculosis mutants |
title |
A two-step strategy for the complementation of M: tuberculosis mutants |
spellingShingle |
A two-step strategy for the complementation of M: tuberculosis mutants Movahedzadeh,Farahnaz tuberculosis mutagenesis constitutive expression complementation |
title_short |
A two-step strategy for the complementation of M: tuberculosis mutants |
title_full |
A two-step strategy for the complementation of M: tuberculosis mutants |
title_fullStr |
A two-step strategy for the complementation of M: tuberculosis mutants |
title_full_unstemmed |
A two-step strategy for the complementation of M: tuberculosis mutants |
title_sort |
A two-step strategy for the complementation of M: tuberculosis mutants |
author |
Movahedzadeh,Farahnaz |
author_facet |
Movahedzadeh,Farahnaz Frita,Rosangela Gutka,Hiten J. |
author_role |
author |
author2 |
Frita,Rosangela Gutka,Hiten J. |
author2_role |
author author |
dc.contributor.author.fl_str_mv |
Movahedzadeh,Farahnaz Frita,Rosangela Gutka,Hiten J. |
dc.subject.por.fl_str_mv |
tuberculosis mutagenesis constitutive expression complementation |
topic |
tuberculosis mutagenesis constitutive expression complementation |
description |
The sequence of Mycobacterium tuberculosis, completed in 1998, facilitated both the development of genomic tools, and the creation of a number of mycobacterial mutants. These mutants have a wide range of phenotypes, from attenuated to hypervirulent strains. These phenotypes must be confirmed, to rule out possible secondary mutations that may arise during the generation of mutant strains. This may occur during the amplification of target genes or during the generation of the mutation, thus constructing a complementation strain, which expresses the wild-type copy of the gene in the mutant strain, becomes necessary. In this study we have introduced a two-step strategy to construct complementation strains using the Ag85 promoter. We have constitutively expressed dosR and have shown dosR expression is restored to wild-type level. |
publishDate |
2011 |
dc.date.none.fl_str_mv |
2011-01-01 |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572011000200020 |
url |
http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572011000200020 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
10.1590/S1415-47572011000200020 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
text/html |
dc.publisher.none.fl_str_mv |
Sociedade Brasileira de Genética |
publisher.none.fl_str_mv |
Sociedade Brasileira de Genética |
dc.source.none.fl_str_mv |
Genetics and Molecular Biology v.34 n.2 2011 reponame:Genetics and Molecular Biology instname:Sociedade Brasileira de Genética (SBG) instacron:SBG |
instname_str |
Sociedade Brasileira de Genética (SBG) |
instacron_str |
SBG |
institution |
SBG |
reponame_str |
Genetics and Molecular Biology |
collection |
Genetics and Molecular Biology |
repository.name.fl_str_mv |
Genetics and Molecular Biology - Sociedade Brasileira de Genética (SBG) |
repository.mail.fl_str_mv |
||editor@gmb.org.br |
_version_ |
1752122383731261440 |