Molecular manipulation and modification of the genes encoding the G2 and G4 glycinin subunits

Detalhes bibliográficos
Autor(a) principal: Sammour,Reda H.
Data de Publicação: 2006
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Genetics and Molecular Biology
Texto Completo: http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572006000300025
Resumo: The genes encoding the glycinin subunits G2 and G4 were molecularly manipulated and modified to test the possibility of increasing the nutritional value of soybean seed proteins. The recombinant DNAs pSP65/G2HG4, pSP65/G4HG2, pSP65/248 Metl, pSP65/248 Met2,3 and pSP65/248 Metl.2,3 were used in in vitro translation to produce (i) chimeric proteins consisting of reciprocally exchanged acidic and basic G2 and G4 domains and (ii) Gy4 point mutants with an increased number of methionine residues. The ability of the recombinant proteins to assemble into proper quaternary structures was investigated using sucrose gradient fractionation. The data produced by this study could provide valuable clues for the potential improvement of genetically modified crops.
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spelling Molecular manipulation and modification of the genes encoding the G2 and G4 glycinin subunitscDNAglycinin subunit G4in vitro translationmutagenesissoybeanThe genes encoding the glycinin subunits G2 and G4 were molecularly manipulated and modified to test the possibility of increasing the nutritional value of soybean seed proteins. The recombinant DNAs pSP65/G2HG4, pSP65/G4HG2, pSP65/248 Metl, pSP65/248 Met2,3 and pSP65/248 Metl.2,3 were used in in vitro translation to produce (i) chimeric proteins consisting of reciprocally exchanged acidic and basic G2 and G4 domains and (ii) Gy4 point mutants with an increased number of methionine residues. The ability of the recombinant proteins to assemble into proper quaternary structures was investigated using sucrose gradient fractionation. The data produced by this study could provide valuable clues for the potential improvement of genetically modified crops.Sociedade Brasileira de Genética2006-01-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersiontext/htmlhttp://old.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572006000300025Genetics and Molecular Biology v.29 n.3 2006reponame:Genetics and Molecular Biologyinstname:Sociedade Brasileira de Genética (SBG)instacron:SBG10.1590/S1415-47572006000300025info:eu-repo/semantics/openAccessSammour,Reda H.eng2006-09-01T00:00:00Zoai:scielo:S1415-47572006000300025Revistahttp://www.gmb.org.br/ONGhttps://old.scielo.br/oai/scielo-oai.php||editor@gmb.org.br1678-46851415-4757opendoar:2006-09-01T00:00Genetics and Molecular Biology - Sociedade Brasileira de Genética (SBG)false
dc.title.none.fl_str_mv Molecular manipulation and modification of the genes encoding the G2 and G4 glycinin subunits
title Molecular manipulation and modification of the genes encoding the G2 and G4 glycinin subunits
spellingShingle Molecular manipulation and modification of the genes encoding the G2 and G4 glycinin subunits
Sammour,Reda H.
cDNA
glycinin subunit G4
in vitro translation
mutagenesis
soybean
title_short Molecular manipulation and modification of the genes encoding the G2 and G4 glycinin subunits
title_full Molecular manipulation and modification of the genes encoding the G2 and G4 glycinin subunits
title_fullStr Molecular manipulation and modification of the genes encoding the G2 and G4 glycinin subunits
title_full_unstemmed Molecular manipulation and modification of the genes encoding the G2 and G4 glycinin subunits
title_sort Molecular manipulation and modification of the genes encoding the G2 and G4 glycinin subunits
author Sammour,Reda H.
author_facet Sammour,Reda H.
author_role author
dc.contributor.author.fl_str_mv Sammour,Reda H.
dc.subject.por.fl_str_mv cDNA
glycinin subunit G4
in vitro translation
mutagenesis
soybean
topic cDNA
glycinin subunit G4
in vitro translation
mutagenesis
soybean
description The genes encoding the glycinin subunits G2 and G4 were molecularly manipulated and modified to test the possibility of increasing the nutritional value of soybean seed proteins. The recombinant DNAs pSP65/G2HG4, pSP65/G4HG2, pSP65/248 Metl, pSP65/248 Met2,3 and pSP65/248 Metl.2,3 were used in in vitro translation to produce (i) chimeric proteins consisting of reciprocally exchanged acidic and basic G2 and G4 domains and (ii) Gy4 point mutants with an increased number of methionine residues. The ability of the recombinant proteins to assemble into proper quaternary structures was investigated using sucrose gradient fractionation. The data produced by this study could provide valuable clues for the potential improvement of genetically modified crops.
publishDate 2006
dc.date.none.fl_str_mv 2006-01-01
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572006000300025
url http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572006000300025
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv 10.1590/S1415-47572006000300025
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv text/html
dc.publisher.none.fl_str_mv Sociedade Brasileira de Genética
publisher.none.fl_str_mv Sociedade Brasileira de Genética
dc.source.none.fl_str_mv Genetics and Molecular Biology v.29 n.3 2006
reponame:Genetics and Molecular Biology
instname:Sociedade Brasileira de Genética (SBG)
instacron:SBG
instname_str Sociedade Brasileira de Genética (SBG)
instacron_str SBG
institution SBG
reponame_str Genetics and Molecular Biology
collection Genetics and Molecular Biology
repository.name.fl_str_mv Genetics and Molecular Biology - Sociedade Brasileira de Genética (SBG)
repository.mail.fl_str_mv ||editor@gmb.org.br
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