Comparison of an in-house and a commercial enzyme-linked immunosorbent assay (ELISA) for diagnosis of paratuberculosis

Detalhes bibliográficos
Autor(a) principal: Marassi,Carla Dray
Data de Publicação: 2007
Outros Autores: Gonzaga,Janete da Silva, Ristow,Paula, Ferreira,Rachel, Fonseca,Leila Souza, Oelemann,Walter, Lilenbaum,Walter
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Brazilian Journal of Microbiology
Texto Completo: http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822007000100002
Resumo: An in-house PPA-ELISA was compared to a commercial ELISA using a panel of 108 serum samples. In relation to commercial test, in-house assay presented 100% of sensitivity and 83.5% of specificity and a high concordance (kappa> 0.5), which demonstrates that the in-house assay is a valuable tool for use in developing countries.
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spelling Comparison of an in-house and a commercial enzyme-linked immunosorbent assay (ELISA) for diagnosis of paratuberculosisCattleMycobacterium avium subsp. paratuberculosisserologyAn in-house PPA-ELISA was compared to a commercial ELISA using a panel of 108 serum samples. In relation to commercial test, in-house assay presented 100% of sensitivity and 83.5% of specificity and a high concordance (kappa> 0.5), which demonstrates that the in-house assay is a valuable tool for use in developing countries.Sociedade Brasileira de Microbiologia2007-03-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersiontext/htmlhttp://old.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822007000100002Brazilian Journal of Microbiology v.38 n.1 2007reponame:Brazilian Journal of Microbiologyinstname:Sociedade Brasileira de Microbiologia (SBM)instacron:SBM10.1590/S1517-83822007000100002info:eu-repo/semantics/openAccessMarassi,Carla DrayGonzaga,Janete da SilvaRistow,PaulaFerreira,RachelFonseca,Leila SouzaOelemann,WalterLilenbaum,Waltereng2008-02-26T00:00:00Zoai:scielo:S1517-83822007000100002Revistahttps://www.scielo.br/j/bjm/ONGhttps://old.scielo.br/oai/scielo-oai.phpbjm@sbmicrobiologia.org.br||mbmartin@usp.br1678-44051517-8382opendoar:2008-02-26T00:00Brazilian Journal of Microbiology - Sociedade Brasileira de Microbiologia (SBM)false
dc.title.none.fl_str_mv Comparison of an in-house and a commercial enzyme-linked immunosorbent assay (ELISA) for diagnosis of paratuberculosis
title Comparison of an in-house and a commercial enzyme-linked immunosorbent assay (ELISA) for diagnosis of paratuberculosis
spellingShingle Comparison of an in-house and a commercial enzyme-linked immunosorbent assay (ELISA) for diagnosis of paratuberculosis
Marassi,Carla Dray
Cattle
Mycobacterium avium subsp. paratuberculosis
serology
title_short Comparison of an in-house and a commercial enzyme-linked immunosorbent assay (ELISA) for diagnosis of paratuberculosis
title_full Comparison of an in-house and a commercial enzyme-linked immunosorbent assay (ELISA) for diagnosis of paratuberculosis
title_fullStr Comparison of an in-house and a commercial enzyme-linked immunosorbent assay (ELISA) for diagnosis of paratuberculosis
title_full_unstemmed Comparison of an in-house and a commercial enzyme-linked immunosorbent assay (ELISA) for diagnosis of paratuberculosis
title_sort Comparison of an in-house and a commercial enzyme-linked immunosorbent assay (ELISA) for diagnosis of paratuberculosis
author Marassi,Carla Dray
author_facet Marassi,Carla Dray
Gonzaga,Janete da Silva
Ristow,Paula
Ferreira,Rachel
Fonseca,Leila Souza
Oelemann,Walter
Lilenbaum,Walter
author_role author
author2 Gonzaga,Janete da Silva
Ristow,Paula
Ferreira,Rachel
Fonseca,Leila Souza
Oelemann,Walter
Lilenbaum,Walter
author2_role author
author
author
author
author
author
dc.contributor.author.fl_str_mv Marassi,Carla Dray
Gonzaga,Janete da Silva
Ristow,Paula
Ferreira,Rachel
Fonseca,Leila Souza
Oelemann,Walter
Lilenbaum,Walter
dc.subject.por.fl_str_mv Cattle
Mycobacterium avium subsp. paratuberculosis
serology
topic Cattle
Mycobacterium avium subsp. paratuberculosis
serology
description An in-house PPA-ELISA was compared to a commercial ELISA using a panel of 108 serum samples. In relation to commercial test, in-house assay presented 100% of sensitivity and 83.5% of specificity and a high concordance (kappa> 0.5), which demonstrates that the in-house assay is a valuable tool for use in developing countries.
publishDate 2007
dc.date.none.fl_str_mv 2007-03-01
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822007000100002
url http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822007000100002
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv 10.1590/S1517-83822007000100002
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv text/html
dc.publisher.none.fl_str_mv Sociedade Brasileira de Microbiologia
publisher.none.fl_str_mv Sociedade Brasileira de Microbiologia
dc.source.none.fl_str_mv Brazilian Journal of Microbiology v.38 n.1 2007
reponame:Brazilian Journal of Microbiology
instname:Sociedade Brasileira de Microbiologia (SBM)
instacron:SBM
instname_str Sociedade Brasileira de Microbiologia (SBM)
instacron_str SBM
institution SBM
reponame_str Brazilian Journal of Microbiology
collection Brazilian Journal of Microbiology
repository.name.fl_str_mv Brazilian Journal of Microbiology - Sociedade Brasileira de Microbiologia (SBM)
repository.mail.fl_str_mv bjm@sbmicrobiologia.org.br||mbmartin@usp.br
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