Germination and germ tube growth of the arbuscular mycorrhizal fungi Gigaspora albida in different substrates
Autor(a) principal: | |
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Data de Publicação: | 2001 |
Outros Autores: | |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Brazilian Journal of Microbiology |
Texto Completo: | http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822001000400005 |
Resumo: | The role of the substrate on germination of Gigaspora albida Schenck & Smith was investigated. Spores were desinfested with 0.5% sodium hypochlorite (20 min.) and placed on Petri dishes over a Millipore filter, with one of the following media: a- 1% water-agar; b- water-agar + aqueous extract of roots of Panicum miliaceum L.; c- salt medium of Murashige & Skoog (MS) or sterilized sand; and incubated in the dark at room temperature (28ºC ± 2). The experimental design was at random with four treatments and four replicates. Germination was evaluated every 7 days until the 28th day. The water-agar medium was the most feasible for spore germination at the 7th day, followed by the MS medium at the 14th day. Conversely, the sand and the root extract medium did not allow high germination. Spores maintained in water-agar also presented longer germ tubes than spores in the other treatments. Auxiliary cells were observed at the beginning of formation of hyphal branching in all treatments, however they were more numerous in the water-agar medium. |
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Brazilian Journal of Microbiology |
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Germination and germ tube growth of the arbuscular mycorrhizal fungi Gigaspora albida in different substrateswater-agarMS mediumroot extractGlomalesPanicum miliaceumThe role of the substrate on germination of Gigaspora albida Schenck & Smith was investigated. Spores were desinfested with 0.5% sodium hypochlorite (20 min.) and placed on Petri dishes over a Millipore filter, with one of the following media: a- 1% water-agar; b- water-agar + aqueous extract of roots of Panicum miliaceum L.; c- salt medium of Murashige & Skoog (MS) or sterilized sand; and incubated in the dark at room temperature (28ºC ± 2). The experimental design was at random with four treatments and four replicates. Germination was evaluated every 7 days until the 28th day. The water-agar medium was the most feasible for spore germination at the 7th day, followed by the MS medium at the 14th day. Conversely, the sand and the root extract medium did not allow high germination. Spores maintained in water-agar also presented longer germ tubes than spores in the other treatments. Auxiliary cells were observed at the beginning of formation of hyphal branching in all treatments, however they were more numerous in the water-agar medium.Sociedade Brasileira de Microbiologia2001-12-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersiontext/htmlhttp://old.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822001000400005Brazilian Journal of Microbiology v.32 n.4 2001reponame:Brazilian Journal of Microbiologyinstname:Sociedade Brasileira de Microbiologia (SBM)instacron:SBM10.1590/S1517-83822001000400005info:eu-repo/semantics/openAccessMaia,Leonor C.Yano-Melo,Adriana M.eng2002-09-02T00:00:00Zoai:scielo:S1517-83822001000400005Revistahttps://www.scielo.br/j/bjm/ONGhttps://old.scielo.br/oai/scielo-oai.phpbjm@sbmicrobiologia.org.br||mbmartin@usp.br1678-44051517-8382opendoar:2002-09-02T00:00Brazilian Journal of Microbiology - Sociedade Brasileira de Microbiologia (SBM)false |
dc.title.none.fl_str_mv |
Germination and germ tube growth of the arbuscular mycorrhizal fungi Gigaspora albida in different substrates |
title |
Germination and germ tube growth of the arbuscular mycorrhizal fungi Gigaspora albida in different substrates |
spellingShingle |
Germination and germ tube growth of the arbuscular mycorrhizal fungi Gigaspora albida in different substrates Maia,Leonor C. water-agar MS medium root extract Glomales Panicum miliaceum |
title_short |
Germination and germ tube growth of the arbuscular mycorrhizal fungi Gigaspora albida in different substrates |
title_full |
Germination and germ tube growth of the arbuscular mycorrhizal fungi Gigaspora albida in different substrates |
title_fullStr |
Germination and germ tube growth of the arbuscular mycorrhizal fungi Gigaspora albida in different substrates |
title_full_unstemmed |
Germination and germ tube growth of the arbuscular mycorrhizal fungi Gigaspora albida in different substrates |
title_sort |
Germination and germ tube growth of the arbuscular mycorrhizal fungi Gigaspora albida in different substrates |
author |
Maia,Leonor C. |
author_facet |
Maia,Leonor C. Yano-Melo,Adriana M. |
author_role |
author |
author2 |
Yano-Melo,Adriana M. |
author2_role |
author |
dc.contributor.author.fl_str_mv |
Maia,Leonor C. Yano-Melo,Adriana M. |
dc.subject.por.fl_str_mv |
water-agar MS medium root extract Glomales Panicum miliaceum |
topic |
water-agar MS medium root extract Glomales Panicum miliaceum |
description |
The role of the substrate on germination of Gigaspora albida Schenck & Smith was investigated. Spores were desinfested with 0.5% sodium hypochlorite (20 min.) and placed on Petri dishes over a Millipore filter, with one of the following media: a- 1% water-agar; b- water-agar + aqueous extract of roots of Panicum miliaceum L.; c- salt medium of Murashige & Skoog (MS) or sterilized sand; and incubated in the dark at room temperature (28ºC ± 2). The experimental design was at random with four treatments and four replicates. Germination was evaluated every 7 days until the 28th day. The water-agar medium was the most feasible for spore germination at the 7th day, followed by the MS medium at the 14th day. Conversely, the sand and the root extract medium did not allow high germination. Spores maintained in water-agar also presented longer germ tubes than spores in the other treatments. Auxiliary cells were observed at the beginning of formation of hyphal branching in all treatments, however they were more numerous in the water-agar medium. |
publishDate |
2001 |
dc.date.none.fl_str_mv |
2001-12-01 |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822001000400005 |
url |
http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822001000400005 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
10.1590/S1517-83822001000400005 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
text/html |
dc.publisher.none.fl_str_mv |
Sociedade Brasileira de Microbiologia |
publisher.none.fl_str_mv |
Sociedade Brasileira de Microbiologia |
dc.source.none.fl_str_mv |
Brazilian Journal of Microbiology v.32 n.4 2001 reponame:Brazilian Journal of Microbiology instname:Sociedade Brasileira de Microbiologia (SBM) instacron:SBM |
instname_str |
Sociedade Brasileira de Microbiologia (SBM) |
instacron_str |
SBM |
institution |
SBM |
reponame_str |
Brazilian Journal of Microbiology |
collection |
Brazilian Journal of Microbiology |
repository.name.fl_str_mv |
Brazilian Journal of Microbiology - Sociedade Brasileira de Microbiologia (SBM) |
repository.mail.fl_str_mv |
bjm@sbmicrobiologia.org.br||mbmartin@usp.br |
_version_ |
1752122199201808384 |