Cyclodextrin glycosyltransferase from Bacillus licheniformis: optimization of production and its properties
Autor(a) principal: | |
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Data de Publicação: | 2006 |
Outros Autores: | , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Brazilian Journal of Microbiology |
Texto Completo: | http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822006000300022 |
Resumo: | Cyclodextrin glycosyltransferase (EC 2.4.1.19) is an enzyme that produces cyclodextrins from starch via an intramolecular transglycosylation reaction. An alkalophilic Bacillus strain, isolated from cassava peels, was identified as Bacillus licheniformis. CGTase production by this strain was better when potato starch was used as carbon source, followed by cassava starch and amylopectin. Glucose and amylose, on the other hand, acted as synthesis repressors. When the cultivation was supplemented with sodium ions and had the pH adjusted between 6.0 and 9.0, the microorganism maintained the growth and enzyme production capacity. This data is interesting because it contradicts the concept that alkalophilic microorganisms do not grow in this pH range. After ultrafiltration-centrifugation, one protein of 85.2 kDa with CGTase activity was isolated. This protein was identified in plates with starch and phenolphthalein. Determination of the optimum temperature showed higher activities at 25ºC and 55ºC, indicating the possible presence of more than one CGTase in the culture filtrate. Km and Vmax values were 1.77 mg/mL and 0.0263 U/mg protein, respectively, using potato starch as substrate. |
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Brazilian Journal of Microbiology |
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Cyclodextrin glycosyltransferase from Bacillus licheniformis: optimization of production and its propertiescyclodextrin glycosyltransferaseCGTase productionBacilluslicheniformisBacillus beta-CDCyclodextrin glycosyltransferase (EC 2.4.1.19) is an enzyme that produces cyclodextrins from starch via an intramolecular transglycosylation reaction. An alkalophilic Bacillus strain, isolated from cassava peels, was identified as Bacillus licheniformis. CGTase production by this strain was better when potato starch was used as carbon source, followed by cassava starch and amylopectin. Glucose and amylose, on the other hand, acted as synthesis repressors. When the cultivation was supplemented with sodium ions and had the pH adjusted between 6.0 and 9.0, the microorganism maintained the growth and enzyme production capacity. This data is interesting because it contradicts the concept that alkalophilic microorganisms do not grow in this pH range. After ultrafiltration-centrifugation, one protein of 85.2 kDa with CGTase activity was isolated. This protein was identified in plates with starch and phenolphthalein. Determination of the optimum temperature showed higher activities at 25ºC and 55ºC, indicating the possible presence of more than one CGTase in the culture filtrate. Km and Vmax values were 1.77 mg/mL and 0.0263 U/mg protein, respectively, using potato starch as substrate.Sociedade Brasileira de Microbiologia2006-09-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersiontext/htmlhttp://old.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822006000300022Brazilian Journal of Microbiology v.37 n.3 2006reponame:Brazilian Journal of Microbiologyinstname:Sociedade Brasileira de Microbiologia (SBM)instacron:SBM10.1590/S1517-83822006000300022info:eu-repo/semantics/openAccessBonilha,Paulo Roberto MartinsMenocci,VivianGoulart,Antonio JoséPolizeli,Maria de Lourdes Teixeira de MoraesMonti,Rubenseng2006-12-18T00:00:00Zoai:scielo:S1517-83822006000300022Revistahttps://www.scielo.br/j/bjm/ONGhttps://old.scielo.br/oai/scielo-oai.phpbjm@sbmicrobiologia.org.br||mbmartin@usp.br1678-44051517-8382opendoar:2006-12-18T00:00Brazilian Journal of Microbiology - Sociedade Brasileira de Microbiologia (SBM)false |
dc.title.none.fl_str_mv |
Cyclodextrin glycosyltransferase from Bacillus licheniformis: optimization of production and its properties |
title |
Cyclodextrin glycosyltransferase from Bacillus licheniformis: optimization of production and its properties |
spellingShingle |
Cyclodextrin glycosyltransferase from Bacillus licheniformis: optimization of production and its properties Bonilha,Paulo Roberto Martins cyclodextrin glycosyltransferase CGTase production Bacilluslicheniformis Bacillus beta-CD |
title_short |
Cyclodextrin glycosyltransferase from Bacillus licheniformis: optimization of production and its properties |
title_full |
Cyclodextrin glycosyltransferase from Bacillus licheniformis: optimization of production and its properties |
title_fullStr |
Cyclodextrin glycosyltransferase from Bacillus licheniformis: optimization of production and its properties |
title_full_unstemmed |
Cyclodextrin glycosyltransferase from Bacillus licheniformis: optimization of production and its properties |
title_sort |
Cyclodextrin glycosyltransferase from Bacillus licheniformis: optimization of production and its properties |
author |
Bonilha,Paulo Roberto Martins |
author_facet |
Bonilha,Paulo Roberto Martins Menocci,Vivian Goulart,Antonio José Polizeli,Maria de Lourdes Teixeira de Moraes Monti,Rubens |
author_role |
author |
author2 |
Menocci,Vivian Goulart,Antonio José Polizeli,Maria de Lourdes Teixeira de Moraes Monti,Rubens |
author2_role |
author author author author |
dc.contributor.author.fl_str_mv |
Bonilha,Paulo Roberto Martins Menocci,Vivian Goulart,Antonio José Polizeli,Maria de Lourdes Teixeira de Moraes Monti,Rubens |
dc.subject.por.fl_str_mv |
cyclodextrin glycosyltransferase CGTase production Bacilluslicheniformis Bacillus beta-CD |
topic |
cyclodextrin glycosyltransferase CGTase production Bacilluslicheniformis Bacillus beta-CD |
description |
Cyclodextrin glycosyltransferase (EC 2.4.1.19) is an enzyme that produces cyclodextrins from starch via an intramolecular transglycosylation reaction. An alkalophilic Bacillus strain, isolated from cassava peels, was identified as Bacillus licheniformis. CGTase production by this strain was better when potato starch was used as carbon source, followed by cassava starch and amylopectin. Glucose and amylose, on the other hand, acted as synthesis repressors. When the cultivation was supplemented with sodium ions and had the pH adjusted between 6.0 and 9.0, the microorganism maintained the growth and enzyme production capacity. This data is interesting because it contradicts the concept that alkalophilic microorganisms do not grow in this pH range. After ultrafiltration-centrifugation, one protein of 85.2 kDa with CGTase activity was isolated. This protein was identified in plates with starch and phenolphthalein. Determination of the optimum temperature showed higher activities at 25ºC and 55ºC, indicating the possible presence of more than one CGTase in the culture filtrate. Km and Vmax values were 1.77 mg/mL and 0.0263 U/mg protein, respectively, using potato starch as substrate. |
publishDate |
2006 |
dc.date.none.fl_str_mv |
2006-09-01 |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822006000300022 |
url |
http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822006000300022 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
10.1590/S1517-83822006000300022 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
text/html |
dc.publisher.none.fl_str_mv |
Sociedade Brasileira de Microbiologia |
publisher.none.fl_str_mv |
Sociedade Brasileira de Microbiologia |
dc.source.none.fl_str_mv |
Brazilian Journal of Microbiology v.37 n.3 2006 reponame:Brazilian Journal of Microbiology instname:Sociedade Brasileira de Microbiologia (SBM) instacron:SBM |
instname_str |
Sociedade Brasileira de Microbiologia (SBM) |
instacron_str |
SBM |
institution |
SBM |
reponame_str |
Brazilian Journal of Microbiology |
collection |
Brazilian Journal of Microbiology |
repository.name.fl_str_mv |
Brazilian Journal of Microbiology - Sociedade Brasileira de Microbiologia (SBM) |
repository.mail.fl_str_mv |
bjm@sbmicrobiologia.org.br||mbmartin@usp.br |
_version_ |
1752122200625774592 |