Partial characterization of cold active amylases and proteases of Streptomyces sp. from Antarctica
Autor(a) principal: | |
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Data de Publicação: | 2011 |
Outros Autores: | , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Brazilian Journal of Microbiology |
Texto Completo: | http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822011000300005 |
Resumo: | The aim of this study was to isolate novel enzyme-producing bacteria from vegetation samples from East Antarctica and also to characterize them genetically and biochemically in order to establish their phylogeny. The ability to grow at low temperature and to produce amylases and proteases cold-active was also tested. The results of the 16S rRNA gene sequence analysis showed that the 4 Alga rRNA was 100% identical to the sequences of Streptomyces sp. rRNA from Norway and from the Solomon Islands. The Streptomyces grew well in submerged system at 20ºC, cells multiplication up to stationary phase being drastically increased after 120 h of submerged cultivation. The beta-amylase production reached a maximum peak after seven days, while alpha-amylase and proteases were performing biosynthesis after nine days of submerged cultivation at 20ºC. Newly Streptomyces were able to produce amylase and proteases in a cold environment. The ability to adapt to low temperature of these enzymes could make them valuable ingredients for detergents, the food industry and bioremediation processes which require low temperatures. |
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Brazilian Journal of Microbiology |
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Partial characterization of cold active amylases and proteases of Streptomyces sp. from AntarcticaStreptomyces spEast Antarcticacold-adapted straincold active proteases and amylaseThe aim of this study was to isolate novel enzyme-producing bacteria from vegetation samples from East Antarctica and also to characterize them genetically and biochemically in order to establish their phylogeny. The ability to grow at low temperature and to produce amylases and proteases cold-active was also tested. The results of the 16S rRNA gene sequence analysis showed that the 4 Alga rRNA was 100% identical to the sequences of Streptomyces sp. rRNA from Norway and from the Solomon Islands. The Streptomyces grew well in submerged system at 20ºC, cells multiplication up to stationary phase being drastically increased after 120 h of submerged cultivation. The beta-amylase production reached a maximum peak after seven days, while alpha-amylase and proteases were performing biosynthesis after nine days of submerged cultivation at 20ºC. Newly Streptomyces were able to produce amylase and proteases in a cold environment. The ability to adapt to low temperature of these enzymes could make them valuable ingredients for detergents, the food industry and bioremediation processes which require low temperatures.Sociedade Brasileira de Microbiologia2011-09-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersiontext/htmlhttp://old.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822011000300005Brazilian Journal of Microbiology v.42 n.3 2011reponame:Brazilian Journal of Microbiologyinstname:Sociedade Brasileira de Microbiologia (SBM)instacron:SBM10.1590/S1517-83822011000300005info:eu-repo/semantics/openAccessCotârleţ,MihaelaNegoiţă,Teodor GhBahrim,Gabriela EStougaard,Petereng2011-12-21T00:00:00Zoai:scielo:S1517-83822011000300005Revistahttps://www.scielo.br/j/bjm/ONGhttps://old.scielo.br/oai/scielo-oai.phpbjm@sbmicrobiologia.org.br||mbmartin@usp.br1678-44051517-8382opendoar:2011-12-21T00:00Brazilian Journal of Microbiology - Sociedade Brasileira de Microbiologia (SBM)false |
dc.title.none.fl_str_mv |
Partial characterization of cold active amylases and proteases of Streptomyces sp. from Antarctica |
title |
Partial characterization of cold active amylases and proteases of Streptomyces sp. from Antarctica |
spellingShingle |
Partial characterization of cold active amylases and proteases of Streptomyces sp. from Antarctica Cotârleţ,Mihaela Streptomyces sp East Antarctica cold-adapted strain cold active proteases and amylase |
title_short |
Partial characterization of cold active amylases and proteases of Streptomyces sp. from Antarctica |
title_full |
Partial characterization of cold active amylases and proteases of Streptomyces sp. from Antarctica |
title_fullStr |
Partial characterization of cold active amylases and proteases of Streptomyces sp. from Antarctica |
title_full_unstemmed |
Partial characterization of cold active amylases and proteases of Streptomyces sp. from Antarctica |
title_sort |
Partial characterization of cold active amylases and proteases of Streptomyces sp. from Antarctica |
author |
Cotârleţ,Mihaela |
author_facet |
Cotârleţ,Mihaela Negoiţă,Teodor Gh Bahrim,Gabriela E Stougaard,Peter |
author_role |
author |
author2 |
Negoiţă,Teodor Gh Bahrim,Gabriela E Stougaard,Peter |
author2_role |
author author author |
dc.contributor.author.fl_str_mv |
Cotârleţ,Mihaela Negoiţă,Teodor Gh Bahrim,Gabriela E Stougaard,Peter |
dc.subject.por.fl_str_mv |
Streptomyces sp East Antarctica cold-adapted strain cold active proteases and amylase |
topic |
Streptomyces sp East Antarctica cold-adapted strain cold active proteases and amylase |
description |
The aim of this study was to isolate novel enzyme-producing bacteria from vegetation samples from East Antarctica and also to characterize them genetically and biochemically in order to establish their phylogeny. The ability to grow at low temperature and to produce amylases and proteases cold-active was also tested. The results of the 16S rRNA gene sequence analysis showed that the 4 Alga rRNA was 100% identical to the sequences of Streptomyces sp. rRNA from Norway and from the Solomon Islands. The Streptomyces grew well in submerged system at 20ºC, cells multiplication up to stationary phase being drastically increased after 120 h of submerged cultivation. The beta-amylase production reached a maximum peak after seven days, while alpha-amylase and proteases were performing biosynthesis after nine days of submerged cultivation at 20ºC. Newly Streptomyces were able to produce amylase and proteases in a cold environment. The ability to adapt to low temperature of these enzymes could make them valuable ingredients for detergents, the food industry and bioremediation processes which require low temperatures. |
publishDate |
2011 |
dc.date.none.fl_str_mv |
2011-09-01 |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822011000300005 |
url |
http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822011000300005 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
10.1590/S1517-83822011000300005 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
text/html |
dc.publisher.none.fl_str_mv |
Sociedade Brasileira de Microbiologia |
publisher.none.fl_str_mv |
Sociedade Brasileira de Microbiologia |
dc.source.none.fl_str_mv |
Brazilian Journal of Microbiology v.42 n.3 2011 reponame:Brazilian Journal of Microbiology instname:Sociedade Brasileira de Microbiologia (SBM) instacron:SBM |
instname_str |
Sociedade Brasileira de Microbiologia (SBM) |
instacron_str |
SBM |
institution |
SBM |
reponame_str |
Brazilian Journal of Microbiology |
collection |
Brazilian Journal of Microbiology |
repository.name.fl_str_mv |
Brazilian Journal of Microbiology - Sociedade Brasileira de Microbiologia (SBM) |
repository.mail.fl_str_mv |
bjm@sbmicrobiologia.org.br||mbmartin@usp.br |
_version_ |
1752122203862728704 |