Phage Therapy as an Approach to Control Salmonella enterica serotype Enteritidis Infection in Mice
Autor(a) principal: | |
---|---|
Data de Publicação: | 2019 |
Outros Autores: | , , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Revista da Sociedade Brasileira de Medicina Tropical |
Texto Completo: | http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0037-86822019000100342 |
Resumo: | Abstract INTRODUCTION: Salmonella enterica serotype Enteritidis (S. Enteritidis) is a cause of food-borne human illness. Given the prevalence of antibiotic resistance of Salmonella Enteritidis and the lack of antibiotic efficacy in future years, its replacement with other agents is necessary. One of the most useful agents is bacteriophages. METHODS S. Enteritidis was identified using a multiplex polymerase chain reaction assay. The effective bacteriophages were isolated from hospital wastewater samples. The effects of the bacteriophages were evaluated both in vitro and in vivo. RESULTS The phage SE20 belonged to the Podoviridae family, and the genome size was 40 kb. The evaluation of phage SE20 at variable pH ranges showed its susceptibility to pH < 3 and pH > 12. The animal model showed that mice infected with S. Enteritidis developed hepatomegaly and splenomegaly, but did not experience gastrointestinal complications after receiving the bacteriophages. CONCLUSIONS The results of this study suggest that phage SE20 is a promising candidate for controlling salmonellosis caused by Salmonella Enteritidis. |
id |
SBMT-1_dd541ed9c2b9a85f342c0411e8e92fec |
---|---|
oai_identifier_str |
oai:scielo:S0037-86822019000100342 |
network_acronym_str |
SBMT-1 |
network_name_str |
Revista da Sociedade Brasileira de Medicina Tropical |
repository_id_str |
|
spelling |
Phage Therapy as an Approach to Control Salmonella enterica serotype Enteritidis Infection in MiceSalmonella enterica serotype EnteritidisPhage SE20Pulsed-field gel electrophoresisMiceSewageAbstract INTRODUCTION: Salmonella enterica serotype Enteritidis (S. Enteritidis) is a cause of food-borne human illness. Given the prevalence of antibiotic resistance of Salmonella Enteritidis and the lack of antibiotic efficacy in future years, its replacement with other agents is necessary. One of the most useful agents is bacteriophages. METHODS S. Enteritidis was identified using a multiplex polymerase chain reaction assay. The effective bacteriophages were isolated from hospital wastewater samples. The effects of the bacteriophages were evaluated both in vitro and in vivo. RESULTS The phage SE20 belonged to the Podoviridae family, and the genome size was 40 kb. The evaluation of phage SE20 at variable pH ranges showed its susceptibility to pH < 3 and pH > 12. The animal model showed that mice infected with S. Enteritidis developed hepatomegaly and splenomegaly, but did not experience gastrointestinal complications after receiving the bacteriophages. CONCLUSIONS The results of this study suggest that phage SE20 is a promising candidate for controlling salmonellosis caused by Salmonella Enteritidis.Sociedade Brasileira de Medicina Tropical - SBMT2019-01-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersiontext/htmlhttp://old.scielo.br/scielo.php?script=sci_arttext&pid=S0037-86822019000100342Revista da Sociedade Brasileira de Medicina Tropical v.52 2019reponame:Revista da Sociedade Brasileira de Medicina Tropicalinstname:Sociedade Brasileira de Medicina Tropical (SBMT)instacron:SBMT10.1590/0037-8682-0290-2019info:eu-repo/semantics/openAccessDallal,Mohammad Mehdi SoltanNikkhahi,FarhadAlimohammadi,MahmoudDouraghi,MasoumehRajabi,ZahraForoushani,Abbas RahimiAzimi,AkramFardsanei,Fatemeheng2019-11-21T00:00:00Zoai:scielo:S0037-86822019000100342Revistahttps://www.sbmt.org.br/portal/revista/ONGhttps://old.scielo.br/oai/scielo-oai.php||dalmo@rsbmt.uftm.edu.br|| rsbmt@rsbmt.uftm.edu.br1678-98490037-8682opendoar:2019-11-21T00:00Revista da Sociedade Brasileira de Medicina Tropical - Sociedade Brasileira de Medicina Tropical (SBMT)false |
dc.title.none.fl_str_mv |
Phage Therapy as an Approach to Control Salmonella enterica serotype Enteritidis Infection in Mice |
title |
Phage Therapy as an Approach to Control Salmonella enterica serotype Enteritidis Infection in Mice |
spellingShingle |
Phage Therapy as an Approach to Control Salmonella enterica serotype Enteritidis Infection in Mice Dallal,Mohammad Mehdi Soltan Salmonella enterica serotype Enteritidis Phage SE20 Pulsed-field gel electrophoresis Mice Sewage |
title_short |
Phage Therapy as an Approach to Control Salmonella enterica serotype Enteritidis Infection in Mice |
title_full |
Phage Therapy as an Approach to Control Salmonella enterica serotype Enteritidis Infection in Mice |
title_fullStr |
Phage Therapy as an Approach to Control Salmonella enterica serotype Enteritidis Infection in Mice |
title_full_unstemmed |
Phage Therapy as an Approach to Control Salmonella enterica serotype Enteritidis Infection in Mice |
title_sort |
Phage Therapy as an Approach to Control Salmonella enterica serotype Enteritidis Infection in Mice |
author |
Dallal,Mohammad Mehdi Soltan |
author_facet |
Dallal,Mohammad Mehdi Soltan Nikkhahi,Farhad Alimohammadi,Mahmoud Douraghi,Masoumeh Rajabi,Zahra Foroushani,Abbas Rahimi Azimi,Akram Fardsanei,Fatemeh |
author_role |
author |
author2 |
Nikkhahi,Farhad Alimohammadi,Mahmoud Douraghi,Masoumeh Rajabi,Zahra Foroushani,Abbas Rahimi Azimi,Akram Fardsanei,Fatemeh |
author2_role |
author author author author author author author |
dc.contributor.author.fl_str_mv |
Dallal,Mohammad Mehdi Soltan Nikkhahi,Farhad Alimohammadi,Mahmoud Douraghi,Masoumeh Rajabi,Zahra Foroushani,Abbas Rahimi Azimi,Akram Fardsanei,Fatemeh |
dc.subject.por.fl_str_mv |
Salmonella enterica serotype Enteritidis Phage SE20 Pulsed-field gel electrophoresis Mice Sewage |
topic |
Salmonella enterica serotype Enteritidis Phage SE20 Pulsed-field gel electrophoresis Mice Sewage |
description |
Abstract INTRODUCTION: Salmonella enterica serotype Enteritidis (S. Enteritidis) is a cause of food-borne human illness. Given the prevalence of antibiotic resistance of Salmonella Enteritidis and the lack of antibiotic efficacy in future years, its replacement with other agents is necessary. One of the most useful agents is bacteriophages. METHODS S. Enteritidis was identified using a multiplex polymerase chain reaction assay. The effective bacteriophages were isolated from hospital wastewater samples. The effects of the bacteriophages were evaluated both in vitro and in vivo. RESULTS The phage SE20 belonged to the Podoviridae family, and the genome size was 40 kb. The evaluation of phage SE20 at variable pH ranges showed its susceptibility to pH < 3 and pH > 12. The animal model showed that mice infected with S. Enteritidis developed hepatomegaly and splenomegaly, but did not experience gastrointestinal complications after receiving the bacteriophages. CONCLUSIONS The results of this study suggest that phage SE20 is a promising candidate for controlling salmonellosis caused by Salmonella Enteritidis. |
publishDate |
2019 |
dc.date.none.fl_str_mv |
2019-01-01 |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0037-86822019000100342 |
url |
http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0037-86822019000100342 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
10.1590/0037-8682-0290-2019 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
text/html |
dc.publisher.none.fl_str_mv |
Sociedade Brasileira de Medicina Tropical - SBMT |
publisher.none.fl_str_mv |
Sociedade Brasileira de Medicina Tropical - SBMT |
dc.source.none.fl_str_mv |
Revista da Sociedade Brasileira de Medicina Tropical v.52 2019 reponame:Revista da Sociedade Brasileira de Medicina Tropical instname:Sociedade Brasileira de Medicina Tropical (SBMT) instacron:SBMT |
instname_str |
Sociedade Brasileira de Medicina Tropical (SBMT) |
instacron_str |
SBMT |
institution |
SBMT |
reponame_str |
Revista da Sociedade Brasileira de Medicina Tropical |
collection |
Revista da Sociedade Brasileira de Medicina Tropical |
repository.name.fl_str_mv |
Revista da Sociedade Brasileira de Medicina Tropical - Sociedade Brasileira de Medicina Tropical (SBMT) |
repository.mail.fl_str_mv |
||dalmo@rsbmt.uftm.edu.br|| rsbmt@rsbmt.uftm.edu.br |
_version_ |
1752122161663836160 |