Ovicidal, pupicidal, adulticidal, and repellent activity of Helicteres velutina K. Schum against Aedes aegypti L. (Diptera: Culicidae)
Autor(a) principal: | |
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Data de Publicação: | 2021 |
Outros Autores: | , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Brazilian Journal of Veterinary Medicine |
Texto Completo: | https://rbmv.org/BJVM/article/view/1121 |
Resumo: | Aedes aegypti is a vector of emerging and neglected diseases, such as dengue, chikungunya, and Zika. Helicteres velutina, known as “pitó” in Brazil, is traditionally used as an insect repellent, and several studies have demonstrated its larvicidal activity. The aim of this study was to investigate this species and evaluate its potential ovicidal, pupicidal, adulticidal, and repellent activity. The viability of the eggs was evaluated using different concentrations of the test substances for 25 days. The hexane fraction killed 72.7% of the eggs, while dichloromethane killed 67.7%. The survival of the pupae and adults was verified after 72 h and 48 h, respectively. The LC50 for the hexane and dichloromethane fractions was 0.12 mg/mL and 8.85 mg/mL for pupae, 8.01 mg/mL and 0.74 mg/mL for adults (tarsal test), and 0.05 mg/mL and 0.23 mg/mL for adults (body test), respectively. Repellency was assessed for 240 min using neonatal Wistar rats on a Y-tube olfactometer. The hexane fraction attracted mosquitoes to the test chamber, while the dichloromethane fraction had a repellent action. The 7,4’-di-O-methyl-8-O-sulfate flavone provides greater repellency, and this finding is similar to the results of the in silico studies that have shown the potential of this substance against adult mosquitoes. This suggests that 7,4’-di-O-methyl-8-O-sulfate flavone may be one of the substances present in the extract from aerial parts of H. velutina that is responsible for the repellent activity mentioned in traditional medicine. These findings provide a better understanding of the insecticidal and repellent activity of the extract, fraction, and compounds isolated from H. velutina against Ae. aegypti, thereby revealing its potential in the development of a more effective botanical insecticide. |
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Ovicidal, pupicidal, adulticidal, and repellent activity of Helicteres velutina K. Schum against Aedes aegypti L. (Diptera: Culicidae)Atividade ovicida, pupicida, adulticida, e repelente de Helicteres velutina K. Schum contra Aedes aegypti L. (Diptera: Culicidae)Helicteres velutinaAtividade inseticidaAtividade repelenteAedes aegyptiDoenças negligenciadasHelicteres velutinaInseticidal activityRepellent activityAedes aegyptiNeglected diseasesAedes aegypti is a vector of emerging and neglected diseases, such as dengue, chikungunya, and Zika. Helicteres velutina, known as “pitó” in Brazil, is traditionally used as an insect repellent, and several studies have demonstrated its larvicidal activity. The aim of this study was to investigate this species and evaluate its potential ovicidal, pupicidal, adulticidal, and repellent activity. The viability of the eggs was evaluated using different concentrations of the test substances for 25 days. The hexane fraction killed 72.7% of the eggs, while dichloromethane killed 67.7%. The survival of the pupae and adults was verified after 72 h and 48 h, respectively. The LC50 for the hexane and dichloromethane fractions was 0.12 mg/mL and 8.85 mg/mL for pupae, 8.01 mg/mL and 0.74 mg/mL for adults (tarsal test), and 0.05 mg/mL and 0.23 mg/mL for adults (body test), respectively. Repellency was assessed for 240 min using neonatal Wistar rats on a Y-tube olfactometer. The hexane fraction attracted mosquitoes to the test chamber, while the dichloromethane fraction had a repellent action. The 7,4’-di-O-methyl-8-O-sulfate flavone provides greater repellency, and this finding is similar to the results of the in silico studies that have shown the potential of this substance against adult mosquitoes. This suggests that 7,4’-di-O-methyl-8-O-sulfate flavone may be one of the substances present in the extract from aerial parts of H. velutina that is responsible for the repellent activity mentioned in traditional medicine. These findings provide a better understanding of the insecticidal and repellent activity of the extract, fraction, and compounds isolated from H. velutina against Ae. aegypti, thereby revealing its potential in the development of a more effective botanical insecticide.Aedes aegypti é o vetor de doenças emergentes e negligenciadas, como dengue, chikungunya e Zika. Helicteres velutina, conhecida como ‘pitó’ no Brasil, é tradicionalmente usada como um repelente de insetos, estudos anteriores comprovaram sua atividade larvicida. O objetivo desta pesquisa foi investigar esta espécie, avaliando seu potencial ovicida, pupicida, adulticida e repelente. A viabilidade dos ovos foi avaliada utilizando diferentes concentrações das substâncias teste durante 25 dias, a fração hexano causou a inviabilização de 72,7% dos ovos, enquanto a diclorometano matou 67,7%. A sobrevivência de pupas e adultos foi verificada após 72 e 48 horas, respectivamente. A CL50 da fração hexano e diclorometano foi de 0,12 e 8,85 mg/mL para pupas; 8,0 e 0,74 mg/mL para adultos (teste tarsal); 0,05 e 0,23 mg/mL para adultos (teste corporal), respectivamente. A repelência foi avaliada durante 240 min utilizando neonatos de ratos Wistar em um olfatômetro de tubo Y. No teste de atração-repelência, a fração hexano atraiu mosquitos para camara teste, enquanto a fração diclorometano causou repelência. A 7,4’-di-O-metil-8-O-sulfato flavona proporcionol maior repelência, corroborando com estudos in silico que mostram potencial dessa substância em mosquitos adultos. Sugerindo que 7,4’-di-O-metil-8-O-sulfato flavona pode ser uma das substâncias presente no extrato das partes aéreas de H. velutina, responsável pela atividade repelente citada na medicina tradicional. Esses achados proporcionam uma melhor compreensão da atividade inseticida e repelente do extrato, fração e compostos isolados de H. velutina frente a Ae. aegypti mostrando potencial no desenvolvimento de um inseticida botânico mais eficaz.Sociedade de Medicina Veterinária do Estado do Rio de Janeiro.2021-03-26info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionpeer reviewedAvaliado pelos paresapplication/pdfhttps://rbmv.org/BJVM/article/view/112110.29374/2527-2179.bjvm112120Brazilian Journal of Veterinary Medicine; Vol. 43 No. 1 (2021); e102120Revista Brasileira de Medicina Veterinária; v. 43 n. 1 (2021); e1021202527-21790100-2430reponame:Brazilian Journal of Veterinary Medicineinstname:Sociedade de Medicina Veterinária do Estado do Rio de Janeiro (SOMVERJ)instacron:SBMVenghttps://rbmv.org/BJVM/article/view/1121/1077Copyright (c) 2021 Diegina Araújo Fernandes, Hyago Luiz Rique, Louise Helena Guimarães de Oliveira, Wilias Greison Silva Santos, Maria de Fátima Vanderlei de Souza, Fabiola da Cruz Nuneshttp://creativecommons.org/licenses/by/4.0info:eu-repo/semantics/openAccessAraújo Fernandes, DieginaLuiz Rique, HyagoGuimarães de Oliveira, Louise HelenaGreison Silva Santos, WiliasVanderlei de Souza, Maria de Fátimada Cruz Nunes, Fabiola2021-05-23T22:36:59Zoai:ojs.rbmv.org:article/1121Revistahttps://rbmv.org/BJVMONGhttps://rbmv.org/BJVM/oaicontato.rbmv@gmail.com2527-21790100-2430opendoar:2021-05-23T22:36:59Brazilian Journal of Veterinary Medicine - Sociedade de Medicina Veterinária do Estado do Rio de Janeiro (SOMVERJ)false |
dc.title.none.fl_str_mv |
Ovicidal, pupicidal, adulticidal, and repellent activity of Helicteres velutina K. Schum against Aedes aegypti L. (Diptera: Culicidae) Atividade ovicida, pupicida, adulticida, e repelente de Helicteres velutina K. Schum contra Aedes aegypti L. (Diptera: Culicidae) |
title |
Ovicidal, pupicidal, adulticidal, and repellent activity of Helicteres velutina K. Schum against Aedes aegypti L. (Diptera: Culicidae) |
spellingShingle |
Ovicidal, pupicidal, adulticidal, and repellent activity of Helicteres velutina K. Schum against Aedes aegypti L. (Diptera: Culicidae) Araújo Fernandes, Diegina Helicteres velutina Atividade inseticida Atividade repelente Aedes aegypti Doenças negligenciadas Helicteres velutina Inseticidal activity Repellent activity Aedes aegypti Neglected diseases |
title_short |
Ovicidal, pupicidal, adulticidal, and repellent activity of Helicteres velutina K. Schum against Aedes aegypti L. (Diptera: Culicidae) |
title_full |
Ovicidal, pupicidal, adulticidal, and repellent activity of Helicteres velutina K. Schum against Aedes aegypti L. (Diptera: Culicidae) |
title_fullStr |
Ovicidal, pupicidal, adulticidal, and repellent activity of Helicteres velutina K. Schum against Aedes aegypti L. (Diptera: Culicidae) |
title_full_unstemmed |
Ovicidal, pupicidal, adulticidal, and repellent activity of Helicteres velutina K. Schum against Aedes aegypti L. (Diptera: Culicidae) |
title_sort |
Ovicidal, pupicidal, adulticidal, and repellent activity of Helicteres velutina K. Schum against Aedes aegypti L. (Diptera: Culicidae) |
author |
Araújo Fernandes, Diegina |
author_facet |
Araújo Fernandes, Diegina Luiz Rique, Hyago Guimarães de Oliveira, Louise Helena Greison Silva Santos, Wilias Vanderlei de Souza, Maria de Fátima da Cruz Nunes, Fabiola |
author_role |
author |
author2 |
Luiz Rique, Hyago Guimarães de Oliveira, Louise Helena Greison Silva Santos, Wilias Vanderlei de Souza, Maria de Fátima da Cruz Nunes, Fabiola |
author2_role |
author author author author author |
dc.contributor.author.fl_str_mv |
Araújo Fernandes, Diegina Luiz Rique, Hyago Guimarães de Oliveira, Louise Helena Greison Silva Santos, Wilias Vanderlei de Souza, Maria de Fátima da Cruz Nunes, Fabiola |
dc.subject.por.fl_str_mv |
Helicteres velutina Atividade inseticida Atividade repelente Aedes aegypti Doenças negligenciadas Helicteres velutina Inseticidal activity Repellent activity Aedes aegypti Neglected diseases |
topic |
Helicteres velutina Atividade inseticida Atividade repelente Aedes aegypti Doenças negligenciadas Helicteres velutina Inseticidal activity Repellent activity Aedes aegypti Neglected diseases |
description |
Aedes aegypti is a vector of emerging and neglected diseases, such as dengue, chikungunya, and Zika. Helicteres velutina, known as “pitó” in Brazil, is traditionally used as an insect repellent, and several studies have demonstrated its larvicidal activity. The aim of this study was to investigate this species and evaluate its potential ovicidal, pupicidal, adulticidal, and repellent activity. The viability of the eggs was evaluated using different concentrations of the test substances for 25 days. The hexane fraction killed 72.7% of the eggs, while dichloromethane killed 67.7%. The survival of the pupae and adults was verified after 72 h and 48 h, respectively. The LC50 for the hexane and dichloromethane fractions was 0.12 mg/mL and 8.85 mg/mL for pupae, 8.01 mg/mL and 0.74 mg/mL for adults (tarsal test), and 0.05 mg/mL and 0.23 mg/mL for adults (body test), respectively. Repellency was assessed for 240 min using neonatal Wistar rats on a Y-tube olfactometer. The hexane fraction attracted mosquitoes to the test chamber, while the dichloromethane fraction had a repellent action. The 7,4’-di-O-methyl-8-O-sulfate flavone provides greater repellency, and this finding is similar to the results of the in silico studies that have shown the potential of this substance against adult mosquitoes. This suggests that 7,4’-di-O-methyl-8-O-sulfate flavone may be one of the substances present in the extract from aerial parts of H. velutina that is responsible for the repellent activity mentioned in traditional medicine. These findings provide a better understanding of the insecticidal and repellent activity of the extract, fraction, and compounds isolated from H. velutina against Ae. aegypti, thereby revealing its potential in the development of a more effective botanical insecticide. |
publishDate |
2021 |
dc.date.none.fl_str_mv |
2021-03-26 |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion peer reviewed Avaliado pelos pares |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
https://rbmv.org/BJVM/article/view/1121 10.29374/2527-2179.bjvm112120 |
url |
https://rbmv.org/BJVM/article/view/1121 |
identifier_str_mv |
10.29374/2527-2179.bjvm112120 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
https://rbmv.org/BJVM/article/view/1121/1077 |
dc.rights.driver.fl_str_mv |
http://creativecommons.org/licenses/by/4.0 info:eu-repo/semantics/openAccess |
rights_invalid_str_mv |
http://creativecommons.org/licenses/by/4.0 |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
application/pdf |
dc.publisher.none.fl_str_mv |
Sociedade de Medicina Veterinária do Estado do Rio de Janeiro. |
publisher.none.fl_str_mv |
Sociedade de Medicina Veterinária do Estado do Rio de Janeiro. |
dc.source.none.fl_str_mv |
Brazilian Journal of Veterinary Medicine; Vol. 43 No. 1 (2021); e102120 Revista Brasileira de Medicina Veterinária; v. 43 n. 1 (2021); e102120 2527-2179 0100-2430 reponame:Brazilian Journal of Veterinary Medicine instname:Sociedade de Medicina Veterinária do Estado do Rio de Janeiro (SOMVERJ) instacron:SBMV |
instname_str |
Sociedade de Medicina Veterinária do Estado do Rio de Janeiro (SOMVERJ) |
instacron_str |
SBMV |
institution |
SBMV |
reponame_str |
Brazilian Journal of Veterinary Medicine |
collection |
Brazilian Journal of Veterinary Medicine |
repository.name.fl_str_mv |
Brazilian Journal of Veterinary Medicine - Sociedade de Medicina Veterinária do Estado do Rio de Janeiro (SOMVERJ) |
repository.mail.fl_str_mv |
contato.rbmv@gmail.com |
_version_ |
1798313110676701184 |