Histological evaluation of bovine tunica albuginea as biomaterial conserved in 98% glycerin and in 0.625% glutaraldehyde
Autor(a) principal: | |
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Data de Publicação: | 2015 |
Outros Autores: | , , , , |
Tipo de documento: | Artigo |
Idioma: | por |
Título da fonte: | Brazilian Journal of Veterinary Medicine |
Texto Completo: | https://rbmv.org/BJVM/article/view/415 |
Resumo: | ABSTRACT. Oliveira L.L., Barata J.S., Silva A.V.P., Caravalho E.C.Q., Nunes L.C. & Abílio E.J. [Histological evaluation of bovine tunica albuginea as biomaterial conserved in 98% glycerin and in 0.625% glutaraldehyde.] Avaliação histológica da túnica albugínea bovina como biomaterial conservada em glicerina a 98% e em glutaraldeído a 0,625%. Revista Brasileira de Medicina Veterinária, 37(4):309-315, 2015. Departamento de Medicina Veterinária, Universidade Federal do Espírito Santo, Alto Universitário s/n, Guararema, Alegre, ES 29500-000, Brasil. E-mail: leticialealolive@hotmail.com Biomaterials have long been studied in reconstructive surgery, both natural and synthetic origin. It can be used as support to host tissue cells development and an efficient bed of stem cells for tissue engineering and regeneration of tissues and organs. The aim of this study was to evaluate the tunica albuginea as biomaterial preserved in glycerin 98% and glutaraldehyde 0.625% and analyze its integrity for use in grafting therapy by microscopic examinations. Tunica albuginea were obtained from healthy adults crossbred cattle from slaughterhouse, tunicas albugineas were preserved in 98% glycerin and 0.625% glutaraldehyde for a period of 30 days at least. Tunica albuginea was fixed in 10% formalin, histologically processed and stained by hematoxylin-eosin, Masson’s trichrome and red picrosisrius polarization. Macroscopically there was a difference in the texture and color of tunica albuginea and in glutaraldehyde group were more firm and thick. Microscopic evaluation revealed that both the glycerin and the glutaraldehyde can be used as conservation medium in function of preserving the basic architecture tissue of the tunica albuginea, however, the glycerin was more efficient preservation of cellular structures. Histochemical techniques have highlighted the collagen and elastic fibers present in tunicas albugineas and highlight the predominance of type I collagen refringence revealing more pronounced in samples preserved in glycerin. |
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Histological evaluation of bovine tunica albuginea as biomaterial conserved in 98% glycerin and in 0.625% glutaraldehydeAvaliação histológica da túnica albugínea bovina como biomaterial conservada em glicerina a 98% e em glutaraldeído a 0,625%Túnica albugíneabiomaterialglicerina a 98%glutaraldeído a 0,625%Tunica albugineabiomaterial98% glycerin0.625% glutaraldehydeABSTRACT. Oliveira L.L., Barata J.S., Silva A.V.P., Caravalho E.C.Q., Nunes L.C. & Abílio E.J. [Histological evaluation of bovine tunica albuginea as biomaterial conserved in 98% glycerin and in 0.625% glutaraldehyde.] Avaliação histológica da túnica albugínea bovina como biomaterial conservada em glicerina a 98% e em glutaraldeído a 0,625%. Revista Brasileira de Medicina Veterinária, 37(4):309-315, 2015. Departamento de Medicina Veterinária, Universidade Federal do Espírito Santo, Alto Universitário s/n, Guararema, Alegre, ES 29500-000, Brasil. E-mail: leticialealolive@hotmail.com Biomaterials have long been studied in reconstructive surgery, both natural and synthetic origin. It can be used as support to host tissue cells development and an efficient bed of stem cells for tissue engineering and regeneration of tissues and organs. The aim of this study was to evaluate the tunica albuginea as biomaterial preserved in glycerin 98% and glutaraldehyde 0.625% and analyze its integrity for use in grafting therapy by microscopic examinations. Tunica albuginea were obtained from healthy adults crossbred cattle from slaughterhouse, tunicas albugineas were preserved in 98% glycerin and 0.625% glutaraldehyde for a period of 30 days at least. Tunica albuginea was fixed in 10% formalin, histologically processed and stained by hematoxylin-eosin, Masson’s trichrome and red picrosisrius polarization. Macroscopically there was a difference in the texture and color of tunica albuginea and in glutaraldehyde group were more firm and thick. Microscopic evaluation revealed that both the glycerin and the glutaraldehyde can be used as conservation medium in function of preserving the basic architecture tissue of the tunica albuginea, however, the glycerin was more efficient preservation of cellular structures. Histochemical techniques have highlighted the collagen and elastic fibers present in tunicas albugineas and highlight the predominance of type I collagen refringence revealing more pronounced in samples preserved in glycerin.Os biomateriais em cirurgias reconstrutivas, tanto de origem natural como sintética, têm sido muito pesquisados. Além de servir como suporte para que células do tecido anfitrião se desenvolvam, podem funcionar como um eficiente alicerce de células tronco e de bioengenharia tecidual para a regeneração de tecidos e órgãos. O objetivo do presente estudo foi avaliar por meio de exames microscópicos a viabilidade da túnica albugínea como biomaterial conservado em diferentes meios e atestar sua integridade para o uso em cirurgias reconstrutivas. Foram obtidas túnicas albugíneas de bovinos mestiços, sadios, adultos, provenientes de matadouro frigorífico e conservadas em glicerina a 98%, gluraldeído a 0,625% por um período mínimo de 30 dias. O material foi fixado em formol a 10%, processado histologicamente e corado pelos métodos de hematoxilina-eosina, tricrômico de Masson e picrosisrius red polarização. Macroscopicamente houve diferença na textura e coloração das túnicas e as amostras em glutaraldeído mostraram-se mais firmes e espessas. A avaliação microscópica revelou que tanto a glicerina quanto o glutaraldeído podem ser utilizados como meios de preservação da arquitetura tecidual básica da túnica albugínea, entretanto, a glicerina mostrou-se mais eficiente na preservação das estruturas celulares. As técnicas histoquímicas permitiram evidenciar as fibras colá- genas e elásticas presentes nas túnicas e evidenciar a predominância do colágeno tipo I revelando refringência mais acentuada nas amostras conservadas em glicerina.Sociedade de Medicina Veterinária do Estado do Rio de Janeiro.2015-12-08info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionpeer reviewedAvaliado pelos paresapplication/pdfhttps://rbmv.org/BJVM/article/view/415Brazilian Journal of Veterinary Medicine; Vol. 37 No. 4 (2015); 309-315Revista Brasileira de Medicina Veterinária; v. 37 n. 4 (2015); 309-3152527-21790100-2430reponame:Brazilian Journal of Veterinary Medicineinstname:Sociedade de Medicina Veterinária do Estado do Rio de Janeiro (SOMVERJ)instacron:SBMVporhttps://rbmv.org/BJVM/article/view/415/304Oliveira, Leticia LealBarata, Julielton de Souzada Silva, Alexandre Vinícius Pereirade Carvalho, Eulógio Carlos QueirózNunes, Louisiane de CarvalhoAbílio, Edmundo Jorgeinfo:eu-repo/semantics/openAccess2020-12-23T17:33:38Zoai:ojs.rbmv.org:article/415Revistahttps://rbmv.org/BJVMONGhttps://rbmv.org/BJVM/oaicontato.rbmv@gmail.com2527-21790100-2430opendoar:2020-12-23T17:33:38Brazilian Journal of Veterinary Medicine - Sociedade de Medicina Veterinária do Estado do Rio de Janeiro (SOMVERJ)false |
dc.title.none.fl_str_mv |
Histological evaluation of bovine tunica albuginea as biomaterial conserved in 98% glycerin and in 0.625% glutaraldehyde Avaliação histológica da túnica albugínea bovina como biomaterial conservada em glicerina a 98% e em glutaraldeído a 0,625% |
title |
Histological evaluation of bovine tunica albuginea as biomaterial conserved in 98% glycerin and in 0.625% glutaraldehyde |
spellingShingle |
Histological evaluation of bovine tunica albuginea as biomaterial conserved in 98% glycerin and in 0.625% glutaraldehyde Oliveira, Leticia Leal Túnica albugínea biomaterial glicerina a 98% glutaraldeído a 0,625% Tunica albuginea biomaterial 98% glycerin 0.625% glutaraldehyde |
title_short |
Histological evaluation of bovine tunica albuginea as biomaterial conserved in 98% glycerin and in 0.625% glutaraldehyde |
title_full |
Histological evaluation of bovine tunica albuginea as biomaterial conserved in 98% glycerin and in 0.625% glutaraldehyde |
title_fullStr |
Histological evaluation of bovine tunica albuginea as biomaterial conserved in 98% glycerin and in 0.625% glutaraldehyde |
title_full_unstemmed |
Histological evaluation of bovine tunica albuginea as biomaterial conserved in 98% glycerin and in 0.625% glutaraldehyde |
title_sort |
Histological evaluation of bovine tunica albuginea as biomaterial conserved in 98% glycerin and in 0.625% glutaraldehyde |
author |
Oliveira, Leticia Leal |
author_facet |
Oliveira, Leticia Leal Barata, Julielton de Souza da Silva, Alexandre Vinícius Pereira de Carvalho, Eulógio Carlos Queiróz Nunes, Louisiane de Carvalho Abílio, Edmundo Jorge |
author_role |
author |
author2 |
Barata, Julielton de Souza da Silva, Alexandre Vinícius Pereira de Carvalho, Eulógio Carlos Queiróz Nunes, Louisiane de Carvalho Abílio, Edmundo Jorge |
author2_role |
author author author author author |
dc.contributor.author.fl_str_mv |
Oliveira, Leticia Leal Barata, Julielton de Souza da Silva, Alexandre Vinícius Pereira de Carvalho, Eulógio Carlos Queiróz Nunes, Louisiane de Carvalho Abílio, Edmundo Jorge |
dc.subject.por.fl_str_mv |
Túnica albugínea biomaterial glicerina a 98% glutaraldeído a 0,625% Tunica albuginea biomaterial 98% glycerin 0.625% glutaraldehyde |
topic |
Túnica albugínea biomaterial glicerina a 98% glutaraldeído a 0,625% Tunica albuginea biomaterial 98% glycerin 0.625% glutaraldehyde |
description |
ABSTRACT. Oliveira L.L., Barata J.S., Silva A.V.P., Caravalho E.C.Q., Nunes L.C. & Abílio E.J. [Histological evaluation of bovine tunica albuginea as biomaterial conserved in 98% glycerin and in 0.625% glutaraldehyde.] Avaliação histológica da túnica albugínea bovina como biomaterial conservada em glicerina a 98% e em glutaraldeído a 0,625%. Revista Brasileira de Medicina Veterinária, 37(4):309-315, 2015. Departamento de Medicina Veterinária, Universidade Federal do Espírito Santo, Alto Universitário s/n, Guararema, Alegre, ES 29500-000, Brasil. E-mail: leticialealolive@hotmail.com Biomaterials have long been studied in reconstructive surgery, both natural and synthetic origin. It can be used as support to host tissue cells development and an efficient bed of stem cells for tissue engineering and regeneration of tissues and organs. The aim of this study was to evaluate the tunica albuginea as biomaterial preserved in glycerin 98% and glutaraldehyde 0.625% and analyze its integrity for use in grafting therapy by microscopic examinations. Tunica albuginea were obtained from healthy adults crossbred cattle from slaughterhouse, tunicas albugineas were preserved in 98% glycerin and 0.625% glutaraldehyde for a period of 30 days at least. Tunica albuginea was fixed in 10% formalin, histologically processed and stained by hematoxylin-eosin, Masson’s trichrome and red picrosisrius polarization. Macroscopically there was a difference in the texture and color of tunica albuginea and in glutaraldehyde group were more firm and thick. Microscopic evaluation revealed that both the glycerin and the glutaraldehyde can be used as conservation medium in function of preserving the basic architecture tissue of the tunica albuginea, however, the glycerin was more efficient preservation of cellular structures. Histochemical techniques have highlighted the collagen and elastic fibers present in tunicas albugineas and highlight the predominance of type I collagen refringence revealing more pronounced in samples preserved in glycerin. |
publishDate |
2015 |
dc.date.none.fl_str_mv |
2015-12-08 |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion peer reviewed Avaliado pelos pares |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
https://rbmv.org/BJVM/article/view/415 |
url |
https://rbmv.org/BJVM/article/view/415 |
dc.language.iso.fl_str_mv |
por |
language |
por |
dc.relation.none.fl_str_mv |
https://rbmv.org/BJVM/article/view/415/304 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
application/pdf |
dc.publisher.none.fl_str_mv |
Sociedade de Medicina Veterinária do Estado do Rio de Janeiro. |
publisher.none.fl_str_mv |
Sociedade de Medicina Veterinária do Estado do Rio de Janeiro. |
dc.source.none.fl_str_mv |
Brazilian Journal of Veterinary Medicine; Vol. 37 No. 4 (2015); 309-315 Revista Brasileira de Medicina Veterinária; v. 37 n. 4 (2015); 309-315 2527-2179 0100-2430 reponame:Brazilian Journal of Veterinary Medicine instname:Sociedade de Medicina Veterinária do Estado do Rio de Janeiro (SOMVERJ) instacron:SBMV |
instname_str |
Sociedade de Medicina Veterinária do Estado do Rio de Janeiro (SOMVERJ) |
instacron_str |
SBMV |
institution |
SBMV |
reponame_str |
Brazilian Journal of Veterinary Medicine |
collection |
Brazilian Journal of Veterinary Medicine |
repository.name.fl_str_mv |
Brazilian Journal of Veterinary Medicine - Sociedade de Medicina Veterinária do Estado do Rio de Janeiro (SOMVERJ) |
repository.mail.fl_str_mv |
contato.rbmv@gmail.com |
_version_ |
1798313107668336640 |