DIFFERENT SOLUTIONS OF HYPOOSMOTIC TEST FOR RAM SEMEN

• Autor(a) principal: Moura, Luís Cláudio Oliveira
• Data de Publicação: 2010
• Outros Autores: da Silva, Maíra Corona, Snoeck, Paola Pereira das Neves
• Tipo de documento: Artigo
• Idioma: por
• Título da fonte: Brazilian Journal of Veterinary Medicine
• Texto Completo: https://rbmv.org/BJVM/article/view/847
• topic: Solução; pressão osmótica; frutose-citrato de sódio; sacarose; carneiro; Solution; osmotic pressure; fructose-sodium citrate; sucrose; sheep

ABSTRACT. Moura L.C.O., Silva M.C. & Snoeck P.P. das N. [Different solutions of hypoosmotic test for ram semen]. Diferentes soluções de teste hiposmótico para sêmen ovino. Revista Brasileira de Medicina Veterinária, 32(3):146-150, 2010. Departamento de Ciências Agrárias e Ambientais, Universidade Estadual de Santa Cruz, Rodovia Ilhéus-Itabuna, Km 16, Salobrinho, Ilhéus, BA 45662-000, Brasil. E-mail: [e-mail] The hypoosmotic test (HOS) has even used to evaluate functional integrity of the sperm membrane of many domestic mammals species and is based on the fluids exchange from the intracellular with the extracellular medium until a balance is accomplished. The aim of this study was to compare two different solutions of HOS test for fresh and cooled ram semen, using the fructose-sodium citrate and sucrose solutions, both with 100 mOsmol/L. Eight fresh semen samples and 128 cooled semen samples were evaluated. For each semen sample, two aliquots of 25 μL were taken. One aliquot was added in eppendorf, containing 200 μL fructose-sodium citrate and another was added to 200 mL sucrose solution. The two samples were incubated for thirty minutes at 37o C in water bath and then fixed in 300 μL buffered formalin saline solution. The reactive form was observed in a phase-contrast microscope with oil immersion. The percentage of reactive forms, with functionally membrane, was evaluated after the counting of the coiled/bent tails and either after subtracted of the abnormal tails found before the test. To the fresh semen, the fructose-citrate sodium and the sucrose solution were similar (P>0.05), in the recognition of the reactive forms perceptual, when the count formula was applied. When the count formula was not use, was verified that the fructose-citrate sodium were better than the sucrose (P<0.05). The fructose-citrate sodium solution was better to identify maximal swelling spermatozoa than sucrose solution after HOS test in cooled ram semen (P<0.05).