Efficient mispriming during apolipoprotein E genotyping

Detalhes bibliográficos
Autor(a) principal: Souza,Gabriela Merheb de Azevedo
Data de Publicação: 2005
Outros Autores: Romano,Nara Lúcia, Whittle,Martin R.
Tipo de documento: Relatório
Idioma: eng
Título da fonte: Jornal Brasileiro de Patologia e Medicina Laboratorial (Online)
Texto Completo: http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1676-24442005000100007
Resumo: The determination of ApoE genotypes is a common clinical laboratory procedure. The most frequently used method involves a PCR amplification of the target region followed by a restriction enzyme digestion of the resultant amplicon to obtain a genotype-specific restriction fragment pattern. We describe an unexpected and surprising difficulty that was encountered during our routine laboratory casework.
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spelling Efficient mispriming during apolipoprotein E genotypingPolymerase chain reactionApolipoprotein EApoE genotypingThe determination of ApoE genotypes is a common clinical laboratory procedure. The most frequently used method involves a PCR amplification of the target region followed by a restriction enzyme digestion of the resultant amplicon to obtain a genotype-specific restriction fragment pattern. We describe an unexpected and surprising difficulty that was encountered during our routine laboratory casework.Sociedade Brasileira de Patologia Clínica2005-02-01info:eu-repo/semantics/reportinfo:eu-repo/semantics/publishedVersiontext/htmlhttp://old.scielo.br/scielo.php?script=sci_arttext&pid=S1676-24442005000100007Jornal Brasileiro de Patologia e Medicina Laboratorial v.41 n.1 2005reponame:Jornal Brasileiro de Patologia e Medicina Laboratorial (Online)instname:Sociedade Brasileira de Patologia (SBP)instacron:SBP10.1590/S1676-24442005000100007info:eu-repo/semantics/openAccessSouza,Gabriela Merheb de AzevedoRomano,Nara LúciaWhittle,Martin R.eng2005-04-15T00:00:00Zoai:scielo:S1676-24442005000100007Revistahttp://www.scielo.br/jbpmlhttps://old.scielo.br/oai/scielo-oai.php||jbpml@sbpc.org.br1678-47741676-2444opendoar:2005-04-15T00:00Jornal Brasileiro de Patologia e Medicina Laboratorial (Online) - Sociedade Brasileira de Patologia (SBP)false
dc.title.none.fl_str_mv Efficient mispriming during apolipoprotein E genotyping
title Efficient mispriming during apolipoprotein E genotyping
spellingShingle Efficient mispriming during apolipoprotein E genotyping
Souza,Gabriela Merheb de Azevedo
Polymerase chain reaction
Apolipoprotein E
ApoE genotyping
title_short Efficient mispriming during apolipoprotein E genotyping
title_full Efficient mispriming during apolipoprotein E genotyping
title_fullStr Efficient mispriming during apolipoprotein E genotyping
title_full_unstemmed Efficient mispriming during apolipoprotein E genotyping
title_sort Efficient mispriming during apolipoprotein E genotyping
author Souza,Gabriela Merheb de Azevedo
author_facet Souza,Gabriela Merheb de Azevedo
Romano,Nara Lúcia
Whittle,Martin R.
author_role author
author2 Romano,Nara Lúcia
Whittle,Martin R.
author2_role author
author
dc.contributor.author.fl_str_mv Souza,Gabriela Merheb de Azevedo
Romano,Nara Lúcia
Whittle,Martin R.
dc.subject.por.fl_str_mv Polymerase chain reaction
Apolipoprotein E
ApoE genotyping
topic Polymerase chain reaction
Apolipoprotein E
ApoE genotyping
description The determination of ApoE genotypes is a common clinical laboratory procedure. The most frequently used method involves a PCR amplification of the target region followed by a restriction enzyme digestion of the resultant amplicon to obtain a genotype-specific restriction fragment pattern. We describe an unexpected and surprising difficulty that was encountered during our routine laboratory casework.
publishDate 2005
dc.date.none.fl_str_mv 2005-02-01
dc.type.driver.fl_str_mv info:eu-repo/semantics/report
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
format report
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1676-24442005000100007
url http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1676-24442005000100007
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv 10.1590/S1676-24442005000100007
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv text/html
dc.publisher.none.fl_str_mv
Sociedade Brasileira de Patologia Clínica
publisher.none.fl_str_mv
Sociedade Brasileira de Patologia Clínica
dc.source.none.fl_str_mv Jornal Brasileiro de Patologia e Medicina Laboratorial v.41 n.1 2005
reponame:Jornal Brasileiro de Patologia e Medicina Laboratorial (Online)
instname:Sociedade Brasileira de Patologia (SBP)
instacron:SBP
instname_str Sociedade Brasileira de Patologia (SBP)
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reponame_str Jornal Brasileiro de Patologia e Medicina Laboratorial (Online)
collection Jornal Brasileiro de Patologia e Medicina Laboratorial (Online)
repository.name.fl_str_mv Jornal Brasileiro de Patologia e Medicina Laboratorial (Online) - Sociedade Brasileira de Patologia (SBP)
repository.mail.fl_str_mv ||jbpml@sbpc.org.br
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