Digital image analysis of skin neoplasms evaluated by lectin histochemistry: potential marker to biochemical alterations and tumour differential diagnosis
Autor(a) principal: | |
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Data de Publicação: | 2006 |
Outros Autores: | , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Jornal Brasileiro de Patologia e Medicina Laboratorial (Online) |
Texto Completo: | http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1676-24442006000600009 |
Resumo: | The present study aims to evaluate, through lectin histochemistry, the alterations in the expression of cell surface carbohydrate between benign and malignant lesions of skin using computer image analysis. Skin fragments were obtained through biopsies and diagnosed as basal cell carcinoma (BCC), epidermoid carcinoma (EpC), trichoepithelioma (TE), keratoacanthoma (KA), seborrheic keratosis (SK) and actinic keratosis (AK). Lectins Con A, WGA, PNA, UEA-I and LTA were used in histochemistry study. Image analysis was carried out in a workstation using OPTIMAS TM software system. PNA strong binding pattern to studied tumours evidenced the high expression of D-galactose residues in the epidermal neoplasms when compared to other sugars recognized by the other lectins. Among benign neoplasms, KA presented a high expression of glucose/mannose, alpha-fucose and D-galactose residues evidenced by the intense staining of Con A (94.7%), LTA (84.2%) and PNA (89.4%), respectively. Malignant tumours showed distinct binding patterns. EpC presented significant binding only by PNA lectin. BCC was differentially stained in comparison to the staining pattern observed in benign lesions such as TE. Qualitative (lectin histochemistry) and quantitative (digital image analysis) data obtained in this study evidenced those lectins are potential markers to biochemical alterations in skin neoplasms. |
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Digital image analysis of skin neoplasms evaluated by lectin histochemistry: potential marker to biochemical alterations and tumour differential diagnosisBasal cell carcinomaLectinsImage analysisSkin neoplasmsThe present study aims to evaluate, through lectin histochemistry, the alterations in the expression of cell surface carbohydrate between benign and malignant lesions of skin using computer image analysis. Skin fragments were obtained through biopsies and diagnosed as basal cell carcinoma (BCC), epidermoid carcinoma (EpC), trichoepithelioma (TE), keratoacanthoma (KA), seborrheic keratosis (SK) and actinic keratosis (AK). Lectins Con A, WGA, PNA, UEA-I and LTA were used in histochemistry study. Image analysis was carried out in a workstation using OPTIMAS TM software system. PNA strong binding pattern to studied tumours evidenced the high expression of D-galactose residues in the epidermal neoplasms when compared to other sugars recognized by the other lectins. Among benign neoplasms, KA presented a high expression of glucose/mannose, alpha-fucose and D-galactose residues evidenced by the intense staining of Con A (94.7%), LTA (84.2%) and PNA (89.4%), respectively. Malignant tumours showed distinct binding patterns. EpC presented significant binding only by PNA lectin. BCC was differentially stained in comparison to the staining pattern observed in benign lesions such as TE. Qualitative (lectin histochemistry) and quantitative (digital image analysis) data obtained in this study evidenced those lectins are potential markers to biochemical alterations in skin neoplasms.Sociedade Brasileira de Patologia Clínica2006-12-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersiontext/htmlhttp://old.scielo.br/scielo.php?script=sci_arttext&pid=S1676-24442006000600009Jornal Brasileiro de Patologia e Medicina Laboratorial v.42 n.6 2006reponame:Jornal Brasileiro de Patologia e Medicina Laboratorial (Online)instname:Sociedade Brasileira de Patologia (SBP)instacron:SBP10.1590/S1676-24442006000600009info:eu-repo/semantics/openAccessMelo-Júnior,Mario R.Araújo-Filho,Jorge Luiz S.Patu,Vasco José Ramos M.Machado,Marcos Cezar F. de PaulaBeltrão,Eduardo I.C.Carvalho Jr.,Luiz B.eng2007-01-31T00:00:00Zoai:scielo:S1676-24442006000600009Revistahttp://www.scielo.br/jbpmlhttps://old.scielo.br/oai/scielo-oai.php||jbpml@sbpc.org.br1678-47741676-2444opendoar:2007-01-31T00:00Jornal Brasileiro de Patologia e Medicina Laboratorial (Online) - Sociedade Brasileira de Patologia (SBP)false |
dc.title.none.fl_str_mv |
Digital image analysis of skin neoplasms evaluated by lectin histochemistry: potential marker to biochemical alterations and tumour differential diagnosis |
title |
Digital image analysis of skin neoplasms evaluated by lectin histochemistry: potential marker to biochemical alterations and tumour differential diagnosis |
spellingShingle |
Digital image analysis of skin neoplasms evaluated by lectin histochemistry: potential marker to biochemical alterations and tumour differential diagnosis Melo-Júnior,Mario R. Basal cell carcinoma Lectins Image analysis Skin neoplasms |
title_short |
Digital image analysis of skin neoplasms evaluated by lectin histochemistry: potential marker to biochemical alterations and tumour differential diagnosis |
title_full |
Digital image analysis of skin neoplasms evaluated by lectin histochemistry: potential marker to biochemical alterations and tumour differential diagnosis |
title_fullStr |
Digital image analysis of skin neoplasms evaluated by lectin histochemistry: potential marker to biochemical alterations and tumour differential diagnosis |
title_full_unstemmed |
Digital image analysis of skin neoplasms evaluated by lectin histochemistry: potential marker to biochemical alterations and tumour differential diagnosis |
title_sort |
Digital image analysis of skin neoplasms evaluated by lectin histochemistry: potential marker to biochemical alterations and tumour differential diagnosis |
author |
Melo-Júnior,Mario R. |
author_facet |
Melo-Júnior,Mario R. Araújo-Filho,Jorge Luiz S. Patu,Vasco José Ramos M. Machado,Marcos Cezar F. de Paula Beltrão,Eduardo I.C. Carvalho Jr.,Luiz B. |
author_role |
author |
author2 |
Araújo-Filho,Jorge Luiz S. Patu,Vasco José Ramos M. Machado,Marcos Cezar F. de Paula Beltrão,Eduardo I.C. Carvalho Jr.,Luiz B. |
author2_role |
author author author author author |
dc.contributor.author.fl_str_mv |
Melo-Júnior,Mario R. Araújo-Filho,Jorge Luiz S. Patu,Vasco José Ramos M. Machado,Marcos Cezar F. de Paula Beltrão,Eduardo I.C. Carvalho Jr.,Luiz B. |
dc.subject.por.fl_str_mv |
Basal cell carcinoma Lectins Image analysis Skin neoplasms |
topic |
Basal cell carcinoma Lectins Image analysis Skin neoplasms |
description |
The present study aims to evaluate, through lectin histochemistry, the alterations in the expression of cell surface carbohydrate between benign and malignant lesions of skin using computer image analysis. Skin fragments were obtained through biopsies and diagnosed as basal cell carcinoma (BCC), epidermoid carcinoma (EpC), trichoepithelioma (TE), keratoacanthoma (KA), seborrheic keratosis (SK) and actinic keratosis (AK). Lectins Con A, WGA, PNA, UEA-I and LTA were used in histochemistry study. Image analysis was carried out in a workstation using OPTIMAS TM software system. PNA strong binding pattern to studied tumours evidenced the high expression of D-galactose residues in the epidermal neoplasms when compared to other sugars recognized by the other lectins. Among benign neoplasms, KA presented a high expression of glucose/mannose, alpha-fucose and D-galactose residues evidenced by the intense staining of Con A (94.7%), LTA (84.2%) and PNA (89.4%), respectively. Malignant tumours showed distinct binding patterns. EpC presented significant binding only by PNA lectin. BCC was differentially stained in comparison to the staining pattern observed in benign lesions such as TE. Qualitative (lectin histochemistry) and quantitative (digital image analysis) data obtained in this study evidenced those lectins are potential markers to biochemical alterations in skin neoplasms. |
publishDate |
2006 |
dc.date.none.fl_str_mv |
2006-12-01 |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1676-24442006000600009 |
url |
http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1676-24442006000600009 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
10.1590/S1676-24442006000600009 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
text/html |
dc.publisher.none.fl_str_mv |
Sociedade Brasileira de Patologia Clínica |
publisher.none.fl_str_mv |
Sociedade Brasileira de Patologia Clínica |
dc.source.none.fl_str_mv |
Jornal Brasileiro de Patologia e Medicina Laboratorial v.42 n.6 2006 reponame:Jornal Brasileiro de Patologia e Medicina Laboratorial (Online) instname:Sociedade Brasileira de Patologia (SBP) instacron:SBP |
instname_str |
Sociedade Brasileira de Patologia (SBP) |
instacron_str |
SBP |
institution |
SBP |
reponame_str |
Jornal Brasileiro de Patologia e Medicina Laboratorial (Online) |
collection |
Jornal Brasileiro de Patologia e Medicina Laboratorial (Online) |
repository.name.fl_str_mv |
Jornal Brasileiro de Patologia e Medicina Laboratorial (Online) - Sociedade Brasileira de Patologia (SBP) |
repository.mail.fl_str_mv |
||jbpml@sbpc.org.br |
_version_ |
1752122294091644928 |