Effect of multiple freeze-thaw cycles on the stability of positive anti-treponemal serum samples
Autor(a) principal: | |
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Data de Publicação: | 2017 |
Outros Autores: | , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Jornal Brasileiro de Patologia e Medicina Laboratorial (Online) |
Texto Completo: | http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1676-24442017000400246 |
Resumo: | ABSTRACT Introduction: Biological samples have long been used in multiple laboratory investigations, and this procedure has been an issue of concern, as the samples are submitted to repeated freeze-thaw cycles, which may affect the results of a particular immunodiagnostic assay, due to the occurrence of physical damage to the antibody of interest. Objective: This study aimed at investigating the impact of successive freeze-thaw cycles on the stability of serum samples stored at -20ºC regarding the reactivity of anti-treponemal antibodies. Methods: At the Immunology Center-Instituto Adolfo Lutz (IAL), the analyzed serum samples analyzed were prepared and established as reference material for anti-treponemal immunodiagnostic assays. Sera stability was evaluated by chemiluminescence assay in samples submitted to 25 successive freeze-thaw cycles, ranging from 6 to 174 cycles. Results: Neither statistically significant effect on the reactivity of anti-treponemal antibodies (p-value > 0.05), nor adverse effect were observed in weakly reactive samples, such as the occurrence of false-negative results. Conclusion: It was shown that 174 freeze-thaw cycles of anti-treponemal sera did not affect the stability and the quality of samples, when evaluated by chemiluminescence assay. |
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Effect of multiple freeze-thaw cycles on the stability of positive anti-treponemal serum samplesserumantibodiesTreponema pallidumquality controlreactivity-stabilityABSTRACT Introduction: Biological samples have long been used in multiple laboratory investigations, and this procedure has been an issue of concern, as the samples are submitted to repeated freeze-thaw cycles, which may affect the results of a particular immunodiagnostic assay, due to the occurrence of physical damage to the antibody of interest. Objective: This study aimed at investigating the impact of successive freeze-thaw cycles on the stability of serum samples stored at -20ºC regarding the reactivity of anti-treponemal antibodies. Methods: At the Immunology Center-Instituto Adolfo Lutz (IAL), the analyzed serum samples analyzed were prepared and established as reference material for anti-treponemal immunodiagnostic assays. Sera stability was evaluated by chemiluminescence assay in samples submitted to 25 successive freeze-thaw cycles, ranging from 6 to 174 cycles. Results: Neither statistically significant effect on the reactivity of anti-treponemal antibodies (p-value > 0.05), nor adverse effect were observed in weakly reactive samples, such as the occurrence of false-negative results. Conclusion: It was shown that 174 freeze-thaw cycles of anti-treponemal sera did not affect the stability and the quality of samples, when evaluated by chemiluminescence assay.Sociedade Brasileira de Patologia Clínica2017-08-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersiontext/htmlhttp://old.scielo.br/scielo.php?script=sci_arttext&pid=S1676-24442017000400246Jornal Brasileiro de Patologia e Medicina Laboratorial v.53 n.4 2017reponame:Jornal Brasileiro de Patologia e Medicina Laboratorial (Online)instname:Sociedade Brasileira de Patologia (SBP)instacron:SBP10.5935/1676-2444.20170038info:eu-repo/semantics/openAccessCastejon,Marcia JorgeYamashiro,RosemeireOliveira,Camila C.Oliveira,Elaine L.Silveira,Edilene P. R.Oliveira,Carmem Aparecida F.eng2017-09-25T00:00:00Zoai:scielo:S1676-24442017000400246Revistahttp://www.scielo.br/jbpmlhttps://old.scielo.br/oai/scielo-oai.php||jbpml@sbpc.org.br1678-47741676-2444opendoar:2017-09-25T00:00Jornal Brasileiro de Patologia e Medicina Laboratorial (Online) - Sociedade Brasileira de Patologia (SBP)false |
dc.title.none.fl_str_mv |
Effect of multiple freeze-thaw cycles on the stability of positive anti-treponemal serum samples |
title |
Effect of multiple freeze-thaw cycles on the stability of positive anti-treponemal serum samples |
spellingShingle |
Effect of multiple freeze-thaw cycles on the stability of positive anti-treponemal serum samples Castejon,Marcia Jorge serum antibodies Treponema pallidum quality control reactivity-stability |
title_short |
Effect of multiple freeze-thaw cycles on the stability of positive anti-treponemal serum samples |
title_full |
Effect of multiple freeze-thaw cycles on the stability of positive anti-treponemal serum samples |
title_fullStr |
Effect of multiple freeze-thaw cycles on the stability of positive anti-treponemal serum samples |
title_full_unstemmed |
Effect of multiple freeze-thaw cycles on the stability of positive anti-treponemal serum samples |
title_sort |
Effect of multiple freeze-thaw cycles on the stability of positive anti-treponemal serum samples |
author |
Castejon,Marcia Jorge |
author_facet |
Castejon,Marcia Jorge Yamashiro,Rosemeire Oliveira,Camila C. Oliveira,Elaine L. Silveira,Edilene P. R. Oliveira,Carmem Aparecida F. |
author_role |
author |
author2 |
Yamashiro,Rosemeire Oliveira,Camila C. Oliveira,Elaine L. Silveira,Edilene P. R. Oliveira,Carmem Aparecida F. |
author2_role |
author author author author author |
dc.contributor.author.fl_str_mv |
Castejon,Marcia Jorge Yamashiro,Rosemeire Oliveira,Camila C. Oliveira,Elaine L. Silveira,Edilene P. R. Oliveira,Carmem Aparecida F. |
dc.subject.por.fl_str_mv |
serum antibodies Treponema pallidum quality control reactivity-stability |
topic |
serum antibodies Treponema pallidum quality control reactivity-stability |
description |
ABSTRACT Introduction: Biological samples have long been used in multiple laboratory investigations, and this procedure has been an issue of concern, as the samples are submitted to repeated freeze-thaw cycles, which may affect the results of a particular immunodiagnostic assay, due to the occurrence of physical damage to the antibody of interest. Objective: This study aimed at investigating the impact of successive freeze-thaw cycles on the stability of serum samples stored at -20ºC regarding the reactivity of anti-treponemal antibodies. Methods: At the Immunology Center-Instituto Adolfo Lutz (IAL), the analyzed serum samples analyzed were prepared and established as reference material for anti-treponemal immunodiagnostic assays. Sera stability was evaluated by chemiluminescence assay in samples submitted to 25 successive freeze-thaw cycles, ranging from 6 to 174 cycles. Results: Neither statistically significant effect on the reactivity of anti-treponemal antibodies (p-value > 0.05), nor adverse effect were observed in weakly reactive samples, such as the occurrence of false-negative results. Conclusion: It was shown that 174 freeze-thaw cycles of anti-treponemal sera did not affect the stability and the quality of samples, when evaluated by chemiluminescence assay. |
publishDate |
2017 |
dc.date.none.fl_str_mv |
2017-08-01 |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1676-24442017000400246 |
url |
http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1676-24442017000400246 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
10.5935/1676-2444.20170038 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
text/html |
dc.publisher.none.fl_str_mv |
Sociedade Brasileira de Patologia Clínica |
publisher.none.fl_str_mv |
Sociedade Brasileira de Patologia Clínica |
dc.source.none.fl_str_mv |
Jornal Brasileiro de Patologia e Medicina Laboratorial v.53 n.4 2017 reponame:Jornal Brasileiro de Patologia e Medicina Laboratorial (Online) instname:Sociedade Brasileira de Patologia (SBP) instacron:SBP |
instname_str |
Sociedade Brasileira de Patologia (SBP) |
instacron_str |
SBP |
institution |
SBP |
reponame_str |
Jornal Brasileiro de Patologia e Medicina Laboratorial (Online) |
collection |
Jornal Brasileiro de Patologia e Medicina Laboratorial (Online) |
repository.name.fl_str_mv |
Jornal Brasileiro de Patologia e Medicina Laboratorial (Online) - Sociedade Brasileira de Patologia (SBP) |
repository.mail.fl_str_mv |
||jbpml@sbpc.org.br |
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1752122296779145216 |