Reporting detection of Chlamydia trachomatisDNA in tissues of neonatal death cases
Autor(a) principal: | |
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Data de Publicação: | 2014 |
Outros Autores: | , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Jornal de Pediatria (Online) |
Texto Completo: | http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0021-75572014000200182 |
Resumo: | OBJECTIVE:to determine whether C. trachomatis was present in neonates with infection, but without an isolated pathogen, who died during the first week of life.METHODS:early neonatal death cases whose causes of death had been previously adjudicated by the institutional mortality committee were randomly selected. End-point and real-time polymerase chain reaction of the C. trachomatis omp1 gene was used to blindly identify the presence of chlamydial DNA in the paraffinized samples of five organs (from authorized autopsies) of each of the dead neonates. Additionally, differential diagnoses were conducted by amplifying a fragment of the 16S rRNA of Mycoplasma spp.RESULTS:in five cases (35.7%), C. trachomatis DNA was found in one or more organs. Severe neonatal infection was present in three cases; one of them corresponded to genotype D of C. trachomatis. Interestingly, another case fulfilled the same criteria but had a positive polymerase chain reaction for Mycoplasma hominis, a pathogen known to produce sepsis in newborns.CONCLUSION:the use of molecular biology techniques in these cases of early infant mortality demonstrated that C. trachomatis could play a role in the development of severe infection and in early neonatal death, similarly to that observed with Mycoplasma hominis. Further study is required to determine the pathogenesis of this perinatal infection. |
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Reporting detection of Chlamydia trachomatisDNA in tissues of neonatal death casesChlamydia trachomatisPolymerase chain reactionNewbornSepsisMortalityOBJECTIVE:to determine whether C. trachomatis was present in neonates with infection, but without an isolated pathogen, who died during the first week of life.METHODS:early neonatal death cases whose causes of death had been previously adjudicated by the institutional mortality committee were randomly selected. End-point and real-time polymerase chain reaction of the C. trachomatis omp1 gene was used to blindly identify the presence of chlamydial DNA in the paraffinized samples of five organs (from authorized autopsies) of each of the dead neonates. Additionally, differential diagnoses were conducted by amplifying a fragment of the 16S rRNA of Mycoplasma spp.RESULTS:in five cases (35.7%), C. trachomatis DNA was found in one or more organs. Severe neonatal infection was present in three cases; one of them corresponded to genotype D of C. trachomatis. Interestingly, another case fulfilled the same criteria but had a positive polymerase chain reaction for Mycoplasma hominis, a pathogen known to produce sepsis in newborns.CONCLUSION:the use of molecular biology techniques in these cases of early infant mortality demonstrated that C. trachomatis could play a role in the development of severe infection and in early neonatal death, similarly to that observed with Mycoplasma hominis. Further study is required to determine the pathogenesis of this perinatal infection.Sociedade Brasileira de Pediatria2014-04-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersiontext/htmlhttp://old.scielo.br/scielo.php?script=sci_arttext&pid=S0021-75572014000200182Jornal de Pediatria v.90 n.2 2014reponame:Jornal de Pediatria (Online)instname:Sociedade Brasileira de Pediatria (SBP)instacron:SBPE10.1016/j.jped.2013.09.002info:eu-repo/semantics/openAccessTrejo,Maria HernandezGonzalez,Norma E. HerreraGuerra,Marcos R. EscobedoCruz,M. de Jesus de HaroMoreno-Verduzco,Elsa R.Lopez-Hurtado,MarcelaGuerra-Infante,Fernando M.eng2015-10-08T00:00:00Zoai:scielo:S0021-75572014000200182Revistahttp://www.jped.com.br/https://old.scielo.br/oai/scielo-oai.php||jped@jped.com.br1678-47820021-7557opendoar:2015-10-08T00:00Jornal de Pediatria (Online) - Sociedade Brasileira de Pediatria (SBP)false |
dc.title.none.fl_str_mv |
Reporting detection of Chlamydia trachomatisDNA in tissues of neonatal death cases |
title |
Reporting detection of Chlamydia trachomatisDNA in tissues of neonatal death cases |
spellingShingle |
Reporting detection of Chlamydia trachomatisDNA in tissues of neonatal death cases Trejo,Maria Hernandez Chlamydia trachomatis Polymerase chain reaction Newborn Sepsis Mortality |
title_short |
Reporting detection of Chlamydia trachomatisDNA in tissues of neonatal death cases |
title_full |
Reporting detection of Chlamydia trachomatisDNA in tissues of neonatal death cases |
title_fullStr |
Reporting detection of Chlamydia trachomatisDNA in tissues of neonatal death cases |
title_full_unstemmed |
Reporting detection of Chlamydia trachomatisDNA in tissues of neonatal death cases |
title_sort |
Reporting detection of Chlamydia trachomatisDNA in tissues of neonatal death cases |
author |
Trejo,Maria Hernandez |
author_facet |
Trejo,Maria Hernandez Gonzalez,Norma E. Herrera Guerra,Marcos R. Escobedo Cruz,M. de Jesus de Haro Moreno-Verduzco,Elsa R. Lopez-Hurtado,Marcela Guerra-Infante,Fernando M. |
author_role |
author |
author2 |
Gonzalez,Norma E. Herrera Guerra,Marcos R. Escobedo Cruz,M. de Jesus de Haro Moreno-Verduzco,Elsa R. Lopez-Hurtado,Marcela Guerra-Infante,Fernando M. |
author2_role |
author author author author author author |
dc.contributor.author.fl_str_mv |
Trejo,Maria Hernandez Gonzalez,Norma E. Herrera Guerra,Marcos R. Escobedo Cruz,M. de Jesus de Haro Moreno-Verduzco,Elsa R. Lopez-Hurtado,Marcela Guerra-Infante,Fernando M. |
dc.subject.por.fl_str_mv |
Chlamydia trachomatis Polymerase chain reaction Newborn Sepsis Mortality |
topic |
Chlamydia trachomatis Polymerase chain reaction Newborn Sepsis Mortality |
description |
OBJECTIVE:to determine whether C. trachomatis was present in neonates with infection, but without an isolated pathogen, who died during the first week of life.METHODS:early neonatal death cases whose causes of death had been previously adjudicated by the institutional mortality committee were randomly selected. End-point and real-time polymerase chain reaction of the C. trachomatis omp1 gene was used to blindly identify the presence of chlamydial DNA in the paraffinized samples of five organs (from authorized autopsies) of each of the dead neonates. Additionally, differential diagnoses were conducted by amplifying a fragment of the 16S rRNA of Mycoplasma spp.RESULTS:in five cases (35.7%), C. trachomatis DNA was found in one or more organs. Severe neonatal infection was present in three cases; one of them corresponded to genotype D of C. trachomatis. Interestingly, another case fulfilled the same criteria but had a positive polymerase chain reaction for Mycoplasma hominis, a pathogen known to produce sepsis in newborns.CONCLUSION:the use of molecular biology techniques in these cases of early infant mortality demonstrated that C. trachomatis could play a role in the development of severe infection and in early neonatal death, similarly to that observed with Mycoplasma hominis. Further study is required to determine the pathogenesis of this perinatal infection. |
publishDate |
2014 |
dc.date.none.fl_str_mv |
2014-04-01 |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0021-75572014000200182 |
url |
http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0021-75572014000200182 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
10.1016/j.jped.2013.09.002 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
text/html |
dc.publisher.none.fl_str_mv |
Sociedade Brasileira de Pediatria |
publisher.none.fl_str_mv |
Sociedade Brasileira de Pediatria |
dc.source.none.fl_str_mv |
Jornal de Pediatria v.90 n.2 2014 reponame:Jornal de Pediatria (Online) instname:Sociedade Brasileira de Pediatria (SBP) instacron:SBPE |
instname_str |
Sociedade Brasileira de Pediatria (SBP) |
instacron_str |
SBPE |
institution |
SBPE |
reponame_str |
Jornal de Pediatria (Online) |
collection |
Jornal de Pediatria (Online) |
repository.name.fl_str_mv |
Jornal de Pediatria (Online) - Sociedade Brasileira de Pediatria (SBP) |
repository.mail.fl_str_mv |
||jped@jped.com.br |
_version_ |
1752122319584624640 |