Evaluation of QuEChERS Sample Preparation for Determination of Avermectins Residues in Ovine Muscle by HPLC-FD and UHPLC-MS/MS
Autor(a) principal: | |
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Data de Publicação: | 2017 |
Outros Autores: | , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Journal of the Brazilian Chemical Society (Online) |
Texto Completo: | http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0103-50532017000500878 |
Resumo: | A fast and sensitive method using high performance liquid chromatography-fluorescence detection (HPLC-FD) and ultra-high performance liquid chromatography tandem mass spectrometry (UHPLC-MS/MS) was developed and validated for the determination of avermectins residues in ovine muscle samples. QuEChERS (quick, easy, cheap, effective, rugged and safe) sample preparation based on acetonitrile extraction followed by partitioning with NaCl and Na2SO4 and dispersive solid-phase extraction (d-SPE) clean-up with C18 was applied. Na2SO4 was used instead of MgSO4 due to lower amounts of co-extractives in the final extract. The procedure was validated according to the Commission Decision 2002/657/EC. The method showed determination coefficients (r2) higher than 0.99, recoveries between 93.2 and 124.3% for spike levels between 0.5 and 2.0 times the maximum residues limit (MRL) values. The repeatability and intermediate precision RSD values ranged from 1 to 19%. Decision limits (CCα) and detection capabilities (CCβ) ranged from 10.7 to 59.4 µg kg-1 and 11.4 to 68.8 µg kg-1, respectively. Method performance was successfully evaluated by analyzing real samples and proficiency test with a z-score in the range of ±1. |
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Evaluation of QuEChERS Sample Preparation for Determination of Avermectins Residues in Ovine Muscle by HPLC-FD and UHPLC-MS/MSveterinary drugs residueliquid chromatographyanimal tissueA fast and sensitive method using high performance liquid chromatography-fluorescence detection (HPLC-FD) and ultra-high performance liquid chromatography tandem mass spectrometry (UHPLC-MS/MS) was developed and validated for the determination of avermectins residues in ovine muscle samples. QuEChERS (quick, easy, cheap, effective, rugged and safe) sample preparation based on acetonitrile extraction followed by partitioning with NaCl and Na2SO4 and dispersive solid-phase extraction (d-SPE) clean-up with C18 was applied. Na2SO4 was used instead of MgSO4 due to lower amounts of co-extractives in the final extract. The procedure was validated according to the Commission Decision 2002/657/EC. The method showed determination coefficients (r2) higher than 0.99, recoveries between 93.2 and 124.3% for spike levels between 0.5 and 2.0 times the maximum residues limit (MRL) values. The repeatability and intermediate precision RSD values ranged from 1 to 19%. Decision limits (CCα) and detection capabilities (CCβ) ranged from 10.7 to 59.4 µg kg-1 and 11.4 to 68.8 µg kg-1, respectively. Method performance was successfully evaluated by analyzing real samples and proficiency test with a z-score in the range of ±1.Sociedade Brasileira de Química2017-05-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersiontext/htmlhttp://old.scielo.br/scielo.php?script=sci_arttext&pid=S0103-50532017000500878Journal of the Brazilian Chemical Society v.28 n.5 2017reponame:Journal of the Brazilian Chemical Society (Online)instname:Sociedade Brasileira de Química (SBQ)instacron:SBQ10.21577/0103-5053.20160240info:eu-repo/semantics/openAccessBandeira,Nelson M. G.Ribeiro,Lucila C.Rizzetti,Tiele M.Martins,Manoel L.Adaime,Martha B.Zanella,RenatoPrestes,Osmar D.eng2017-04-12T00:00:00Zoai:scielo:S0103-50532017000500878Revistahttp://jbcs.sbq.org.brONGhttps://old.scielo.br/oai/scielo-oai.php||office@jbcs.sbq.org.br1678-47900103-5053opendoar:2017-04-12T00:00Journal of the Brazilian Chemical Society (Online) - Sociedade Brasileira de Química (SBQ)false |
dc.title.none.fl_str_mv |
Evaluation of QuEChERS Sample Preparation for Determination of Avermectins Residues in Ovine Muscle by HPLC-FD and UHPLC-MS/MS |
title |
Evaluation of QuEChERS Sample Preparation for Determination of Avermectins Residues in Ovine Muscle by HPLC-FD and UHPLC-MS/MS |
spellingShingle |
Evaluation of QuEChERS Sample Preparation for Determination of Avermectins Residues in Ovine Muscle by HPLC-FD and UHPLC-MS/MS Bandeira,Nelson M. G. veterinary drugs residue liquid chromatography animal tissue |
title_short |
Evaluation of QuEChERS Sample Preparation for Determination of Avermectins Residues in Ovine Muscle by HPLC-FD and UHPLC-MS/MS |
title_full |
Evaluation of QuEChERS Sample Preparation for Determination of Avermectins Residues in Ovine Muscle by HPLC-FD and UHPLC-MS/MS |
title_fullStr |
Evaluation of QuEChERS Sample Preparation for Determination of Avermectins Residues in Ovine Muscle by HPLC-FD and UHPLC-MS/MS |
title_full_unstemmed |
Evaluation of QuEChERS Sample Preparation for Determination of Avermectins Residues in Ovine Muscle by HPLC-FD and UHPLC-MS/MS |
title_sort |
Evaluation of QuEChERS Sample Preparation for Determination of Avermectins Residues in Ovine Muscle by HPLC-FD and UHPLC-MS/MS |
author |
Bandeira,Nelson M. G. |
author_facet |
Bandeira,Nelson M. G. Ribeiro,Lucila C. Rizzetti,Tiele M. Martins,Manoel L. Adaime,Martha B. Zanella,Renato Prestes,Osmar D. |
author_role |
author |
author2 |
Ribeiro,Lucila C. Rizzetti,Tiele M. Martins,Manoel L. Adaime,Martha B. Zanella,Renato Prestes,Osmar D. |
author2_role |
author author author author author author |
dc.contributor.author.fl_str_mv |
Bandeira,Nelson M. G. Ribeiro,Lucila C. Rizzetti,Tiele M. Martins,Manoel L. Adaime,Martha B. Zanella,Renato Prestes,Osmar D. |
dc.subject.por.fl_str_mv |
veterinary drugs residue liquid chromatography animal tissue |
topic |
veterinary drugs residue liquid chromatography animal tissue |
description |
A fast and sensitive method using high performance liquid chromatography-fluorescence detection (HPLC-FD) and ultra-high performance liquid chromatography tandem mass spectrometry (UHPLC-MS/MS) was developed and validated for the determination of avermectins residues in ovine muscle samples. QuEChERS (quick, easy, cheap, effective, rugged and safe) sample preparation based on acetonitrile extraction followed by partitioning with NaCl and Na2SO4 and dispersive solid-phase extraction (d-SPE) clean-up with C18 was applied. Na2SO4 was used instead of MgSO4 due to lower amounts of co-extractives in the final extract. The procedure was validated according to the Commission Decision 2002/657/EC. The method showed determination coefficients (r2) higher than 0.99, recoveries between 93.2 and 124.3% for spike levels between 0.5 and 2.0 times the maximum residues limit (MRL) values. The repeatability and intermediate precision RSD values ranged from 1 to 19%. Decision limits (CCα) and detection capabilities (CCβ) ranged from 10.7 to 59.4 µg kg-1 and 11.4 to 68.8 µg kg-1, respectively. Method performance was successfully evaluated by analyzing real samples and proficiency test with a z-score in the range of ±1. |
publishDate |
2017 |
dc.date.none.fl_str_mv |
2017-05-01 |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0103-50532017000500878 |
url |
http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0103-50532017000500878 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
10.21577/0103-5053.20160240 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
text/html |
dc.publisher.none.fl_str_mv |
Sociedade Brasileira de Química |
publisher.none.fl_str_mv |
Sociedade Brasileira de Química |
dc.source.none.fl_str_mv |
Journal of the Brazilian Chemical Society v.28 n.5 2017 reponame:Journal of the Brazilian Chemical Society (Online) instname:Sociedade Brasileira de Química (SBQ) instacron:SBQ |
instname_str |
Sociedade Brasileira de Química (SBQ) |
instacron_str |
SBQ |
institution |
SBQ |
reponame_str |
Journal of the Brazilian Chemical Society (Online) |
collection |
Journal of the Brazilian Chemical Society (Online) |
repository.name.fl_str_mv |
Journal of the Brazilian Chemical Society (Online) - Sociedade Brasileira de Química (SBQ) |
repository.mail.fl_str_mv |
||office@jbcs.sbq.org.br |
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1750318179540795392 |