Optimization of an amperometric biosensor for the detection of hepatitis C virus using fractional factorial designs

Detalhes bibliográficos
Autor(a) principal: Uliana,Carolina V.
Data de Publicação: 2008
Outros Autores: Riccardi,Carla S., Tognolli,João O., Yamanaka,Hideko
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Journal of the Brazilian Chemical Society (Online)
Texto Completo: http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0103-50532008000400024
Resumo: Fractional factorial design and factorial with center point design were applied to the development of an amperometric biosensor for the detection of the hepatitis C virus. Biomolecules were immobilized by adsorption on graphite electrodes modified with siloxane-poly(propyleneoxide) hybrid matrix prepared using the sol-gel method. Several parameters were optimized, such as the streptavidin concentration at 0.01 mg mL-1 and 1.0% bovine serum albumin, the incubation time of the electrodes in the complementary DNA solution for 30 minutes and a 1:1500 dilution of the avidin-peroxidase conjugate, among others. The application of chemometric studies has been efficient, since the best conditions have been established with a restricted number of experiments, indicating the influence of different factors on the system.
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spelling Optimization of an amperometric biosensor for the detection of hepatitis C virus using fractional factorial designsbiosensorhepatitis C virusfractional factorial designFractional factorial design and factorial with center point design were applied to the development of an amperometric biosensor for the detection of the hepatitis C virus. Biomolecules were immobilized by adsorption on graphite electrodes modified with siloxane-poly(propyleneoxide) hybrid matrix prepared using the sol-gel method. Several parameters were optimized, such as the streptavidin concentration at 0.01 mg mL-1 and 1.0% bovine serum albumin, the incubation time of the electrodes in the complementary DNA solution for 30 minutes and a 1:1500 dilution of the avidin-peroxidase conjugate, among others. The application of chemometric studies has been efficient, since the best conditions have been established with a restricted number of experiments, indicating the influence of different factors on the system.Sociedade Brasileira de Química2008-01-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersiontext/htmlhttp://old.scielo.br/scielo.php?script=sci_arttext&pid=S0103-50532008000400024Journal of the Brazilian Chemical Society v.19 n.4 2008reponame:Journal of the Brazilian Chemical Society (Online)instname:Sociedade Brasileira de Química (SBQ)instacron:SBQ10.1590/S0103-50532008000400024info:eu-repo/semantics/openAccessUliana,Carolina V.Riccardi,Carla S.Tognolli,João O.Yamanaka,Hidekoeng2008-06-11T00:00:00Zoai:scielo:S0103-50532008000400024Revistahttp://jbcs.sbq.org.brONGhttps://old.scielo.br/oai/scielo-oai.php||office@jbcs.sbq.org.br1678-47900103-5053opendoar:2008-06-11T00:00Journal of the Brazilian Chemical Society (Online) - Sociedade Brasileira de Química (SBQ)false
dc.title.none.fl_str_mv Optimization of an amperometric biosensor for the detection of hepatitis C virus using fractional factorial designs
title Optimization of an amperometric biosensor for the detection of hepatitis C virus using fractional factorial designs
spellingShingle Optimization of an amperometric biosensor for the detection of hepatitis C virus using fractional factorial designs
Uliana,Carolina V.
biosensor
hepatitis C virus
fractional factorial design
title_short Optimization of an amperometric biosensor for the detection of hepatitis C virus using fractional factorial designs
title_full Optimization of an amperometric biosensor for the detection of hepatitis C virus using fractional factorial designs
title_fullStr Optimization of an amperometric biosensor for the detection of hepatitis C virus using fractional factorial designs
title_full_unstemmed Optimization of an amperometric biosensor for the detection of hepatitis C virus using fractional factorial designs
title_sort Optimization of an amperometric biosensor for the detection of hepatitis C virus using fractional factorial designs
author Uliana,Carolina V.
author_facet Uliana,Carolina V.
Riccardi,Carla S.
Tognolli,João O.
Yamanaka,Hideko
author_role author
author2 Riccardi,Carla S.
Tognolli,João O.
Yamanaka,Hideko
author2_role author
author
author
dc.contributor.author.fl_str_mv Uliana,Carolina V.
Riccardi,Carla S.
Tognolli,João O.
Yamanaka,Hideko
dc.subject.por.fl_str_mv biosensor
hepatitis C virus
fractional factorial design
topic biosensor
hepatitis C virus
fractional factorial design
description Fractional factorial design and factorial with center point design were applied to the development of an amperometric biosensor for the detection of the hepatitis C virus. Biomolecules were immobilized by adsorption on graphite electrodes modified with siloxane-poly(propyleneoxide) hybrid matrix prepared using the sol-gel method. Several parameters were optimized, such as the streptavidin concentration at 0.01 mg mL-1 and 1.0% bovine serum albumin, the incubation time of the electrodes in the complementary DNA solution for 30 minutes and a 1:1500 dilution of the avidin-peroxidase conjugate, among others. The application of chemometric studies has been efficient, since the best conditions have been established with a restricted number of experiments, indicating the influence of different factors on the system.
publishDate 2008
dc.date.none.fl_str_mv 2008-01-01
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0103-50532008000400024
url http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0103-50532008000400024
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv 10.1590/S0103-50532008000400024
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv text/html
dc.publisher.none.fl_str_mv Sociedade Brasileira de Química
publisher.none.fl_str_mv Sociedade Brasileira de Química
dc.source.none.fl_str_mv Journal of the Brazilian Chemical Society v.19 n.4 2008
reponame:Journal of the Brazilian Chemical Society (Online)
instname:Sociedade Brasileira de Química (SBQ)
instacron:SBQ
instname_str Sociedade Brasileira de Química (SBQ)
instacron_str SBQ
institution SBQ
reponame_str Journal of the Brazilian Chemical Society (Online)
collection Journal of the Brazilian Chemical Society (Online)
repository.name.fl_str_mv Journal of the Brazilian Chemical Society (Online) - Sociedade Brasileira de Química (SBQ)
repository.mail.fl_str_mv ||office@jbcs.sbq.org.br
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