Spectroscopic characterization of the exopolysaccharide of Xanthomonas axonopodis pv. citri in Cu2+ resistance mechanism

Detalhes bibliográficos
Autor(a) principal: Osiro,Denise
Data de Publicação: 2011
Outros Autores: Franco,Roberto W. Assis, Colnago,Luiz Alberto
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Journal of the Brazilian Chemical Society (Online)
Texto Completo: http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0103-50532011000700020
Resumo: We analyzed the role of exopolysaccharide in the Xanthomonas axonopodis pv. citri (Xac) Cu2+ resistance mechanism by Fourier transform infrared (FTIR), electron paramagnetic resonance (EPR) and nuclear magnetic resonance (NMR) spectroscopies. The FTIR data show that cells cultivated in the presence of 0.2 mmol L-1 of CuSO4 produce larger amounts of pyruvated exopolissacharide (EPS) than the ones cultivated in its absence. The EPR data indicate that the amount of Cu2+ decreases with cultivation time. The 13C-CPMAS NMR data also show the complexation of Cu2+ ions to the EPS. The results demonstrate that EPS plays an important role in Xac Cu2+ protection. Both capsular and slime EPS act as an initial protection mechanism, binding free Cu2+ ions, reducing their diffusion and their active transport to the cytoplasm. Cu2+ also induces the production of a highly pyruvated negative EPS, increasing its capture and binding capacity.
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spelling Spectroscopic characterization of the exopolysaccharide of Xanthomonas axonopodis pv. citri in Cu2+ resistance mechanismcitrus cankerX. axonopodis pv. citriexopolysaccharideEPRNMRFTIRWe analyzed the role of exopolysaccharide in the Xanthomonas axonopodis pv. citri (Xac) Cu2+ resistance mechanism by Fourier transform infrared (FTIR), electron paramagnetic resonance (EPR) and nuclear magnetic resonance (NMR) spectroscopies. The FTIR data show that cells cultivated in the presence of 0.2 mmol L-1 of CuSO4 produce larger amounts of pyruvated exopolissacharide (EPS) than the ones cultivated in its absence. The EPR data indicate that the amount of Cu2+ decreases with cultivation time. The 13C-CPMAS NMR data also show the complexation of Cu2+ ions to the EPS. The results demonstrate that EPS plays an important role in Xac Cu2+ protection. Both capsular and slime EPS act as an initial protection mechanism, binding free Cu2+ ions, reducing their diffusion and their active transport to the cytoplasm. Cu2+ also induces the production of a highly pyruvated negative EPS, increasing its capture and binding capacity.Sociedade Brasileira de Química2011-07-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersiontext/htmlhttp://old.scielo.br/scielo.php?script=sci_arttext&pid=S0103-50532011000700020Journal of the Brazilian Chemical Society v.22 n.7 2011reponame:Journal of the Brazilian Chemical Society (Online)instname:Sociedade Brasileira de Química (SBQ)instacron:SBQ10.1590/S0103-50532011000700020info:eu-repo/semantics/openAccessOsiro,DeniseFranco,Roberto W. AssisColnago,Luiz Albertoeng2011-07-22T00:00:00Zoai:scielo:S0103-50532011000700020Revistahttp://jbcs.sbq.org.brONGhttps://old.scielo.br/oai/scielo-oai.php||office@jbcs.sbq.org.br1678-47900103-5053opendoar:2011-07-22T00:00Journal of the Brazilian Chemical Society (Online) - Sociedade Brasileira de Química (SBQ)false
dc.title.none.fl_str_mv Spectroscopic characterization of the exopolysaccharide of Xanthomonas axonopodis pv. citri in Cu2+ resistance mechanism
title Spectroscopic characterization of the exopolysaccharide of Xanthomonas axonopodis pv. citri in Cu2+ resistance mechanism
spellingShingle Spectroscopic characterization of the exopolysaccharide of Xanthomonas axonopodis pv. citri in Cu2+ resistance mechanism
Osiro,Denise
citrus canker
X. axonopodis pv. citri
exopolysaccharide
EPR
NMR
FTIR
title_short Spectroscopic characterization of the exopolysaccharide of Xanthomonas axonopodis pv. citri in Cu2+ resistance mechanism
title_full Spectroscopic characterization of the exopolysaccharide of Xanthomonas axonopodis pv. citri in Cu2+ resistance mechanism
title_fullStr Spectroscopic characterization of the exopolysaccharide of Xanthomonas axonopodis pv. citri in Cu2+ resistance mechanism
title_full_unstemmed Spectroscopic characterization of the exopolysaccharide of Xanthomonas axonopodis pv. citri in Cu2+ resistance mechanism
title_sort Spectroscopic characterization of the exopolysaccharide of Xanthomonas axonopodis pv. citri in Cu2+ resistance mechanism
author Osiro,Denise
author_facet Osiro,Denise
Franco,Roberto W. Assis
Colnago,Luiz Alberto
author_role author
author2 Franco,Roberto W. Assis
Colnago,Luiz Alberto
author2_role author
author
dc.contributor.author.fl_str_mv Osiro,Denise
Franco,Roberto W. Assis
Colnago,Luiz Alberto
dc.subject.por.fl_str_mv citrus canker
X. axonopodis pv. citri
exopolysaccharide
EPR
NMR
FTIR
topic citrus canker
X. axonopodis pv. citri
exopolysaccharide
EPR
NMR
FTIR
description We analyzed the role of exopolysaccharide in the Xanthomonas axonopodis pv. citri (Xac) Cu2+ resistance mechanism by Fourier transform infrared (FTIR), electron paramagnetic resonance (EPR) and nuclear magnetic resonance (NMR) spectroscopies. The FTIR data show that cells cultivated in the presence of 0.2 mmol L-1 of CuSO4 produce larger amounts of pyruvated exopolissacharide (EPS) than the ones cultivated in its absence. The EPR data indicate that the amount of Cu2+ decreases with cultivation time. The 13C-CPMAS NMR data also show the complexation of Cu2+ ions to the EPS. The results demonstrate that EPS plays an important role in Xac Cu2+ protection. Both capsular and slime EPS act as an initial protection mechanism, binding free Cu2+ ions, reducing their diffusion and their active transport to the cytoplasm. Cu2+ also induces the production of a highly pyruvated negative EPS, increasing its capture and binding capacity.
publishDate 2011
dc.date.none.fl_str_mv 2011-07-01
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0103-50532011000700020
url http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0103-50532011000700020
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv 10.1590/S0103-50532011000700020
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv text/html
dc.publisher.none.fl_str_mv Sociedade Brasileira de Química
publisher.none.fl_str_mv Sociedade Brasileira de Química
dc.source.none.fl_str_mv Journal of the Brazilian Chemical Society v.22 n.7 2011
reponame:Journal of the Brazilian Chemical Society (Online)
instname:Sociedade Brasileira de Química (SBQ)
instacron:SBQ
instname_str Sociedade Brasileira de Química (SBQ)
instacron_str SBQ
institution SBQ
reponame_str Journal of the Brazilian Chemical Society (Online)
collection Journal of the Brazilian Chemical Society (Online)
repository.name.fl_str_mv Journal of the Brazilian Chemical Society (Online) - Sociedade Brasileira de Química (SBQ)
repository.mail.fl_str_mv ||office@jbcs.sbq.org.br
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