Um aparato para monitorar reações rápidas: um "stopped-flow" artesanal de baixo custo

Detalhes bibliográficos
Autor(a) principal: Batistela,Vagner Roberto
Data de Publicação: 2006
Outros Autores: Tessaro,André Luiz, Soares,Rafael R. da S., Souza,Vagner Roberto de, Hioka,Noboru
Tipo de documento: Artigo
Idioma: por
Título da fonte: Química Nova (Online)
Texto Completo: http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0100-40422006000500034
Resumo: The modern stopped-flow reaction analyzer has shown high efficiency and flexibility, which provides outstanding sample economy with a dead-time of less than 1 ms. However the cost of the equipment imposes a serious restriction to many Brazilian scientists and teachers. In this work we describe the construction of a low-cost stopped-flow system coupled to a UV-Vis spectrophotometer. The performance of the system was checked by monitoring the kinetics of two reactions: the fading of phenolphthalein in aqueous alkaline solution and the chlorophyll a demetallation in acid medium. The apparatus showed reasonable efficiency with a dead-time of 0.3 to 0.5 s. The very good results obtained in these two illustrative processes show that the system is satisfactory for determining rate constants with mean reaction times ranging from seconds to minutes.
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spelling Um aparato para monitorar reações rápidas: um "stopped-flow" artesanal de baixo custostopped-flowkineticsfast reactionsThe modern stopped-flow reaction analyzer has shown high efficiency and flexibility, which provides outstanding sample economy with a dead-time of less than 1 ms. However the cost of the equipment imposes a serious restriction to many Brazilian scientists and teachers. In this work we describe the construction of a low-cost stopped-flow system coupled to a UV-Vis spectrophotometer. The performance of the system was checked by monitoring the kinetics of two reactions: the fading of phenolphthalein in aqueous alkaline solution and the chlorophyll a demetallation in acid medium. The apparatus showed reasonable efficiency with a dead-time of 0.3 to 0.5 s. The very good results obtained in these two illustrative processes show that the system is satisfactory for determining rate constants with mean reaction times ranging from seconds to minutes.Sociedade Brasileira de Química2006-10-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersiontext/htmlhttp://old.scielo.br/scielo.php?script=sci_arttext&pid=S0100-40422006000500034Química Nova v.29 n.5 2006reponame:Química Nova (Online)instname:Sociedade Brasileira de Química (SBQ)instacron:SBQ10.1590/S0100-40422006000500034info:eu-repo/semantics/openAccessBatistela,Vagner RobertoTessaro,André LuizSoares,Rafael R. da S.Souza,Vagner Roberto deHioka,Noborupor2011-09-06T00:00:00Zoai:scielo:S0100-40422006000500034Revistahttps://www.scielo.br/j/qn/ONGhttps://old.scielo.br/oai/scielo-oai.phpquimicanova@sbq.org.br1678-70640100-4042opendoar:2011-09-06T00:00Química Nova (Online) - Sociedade Brasileira de Química (SBQ)false
dc.title.none.fl_str_mv Um aparato para monitorar reações rápidas: um "stopped-flow" artesanal de baixo custo
title Um aparato para monitorar reações rápidas: um "stopped-flow" artesanal de baixo custo
spellingShingle Um aparato para monitorar reações rápidas: um "stopped-flow" artesanal de baixo custo
Batistela,Vagner Roberto
stopped-flow
kinetics
fast reactions
title_short Um aparato para monitorar reações rápidas: um "stopped-flow" artesanal de baixo custo
title_full Um aparato para monitorar reações rápidas: um "stopped-flow" artesanal de baixo custo
title_fullStr Um aparato para monitorar reações rápidas: um "stopped-flow" artesanal de baixo custo
title_full_unstemmed Um aparato para monitorar reações rápidas: um "stopped-flow" artesanal de baixo custo
title_sort Um aparato para monitorar reações rápidas: um "stopped-flow" artesanal de baixo custo
author Batistela,Vagner Roberto
author_facet Batistela,Vagner Roberto
Tessaro,André Luiz
Soares,Rafael R. da S.
Souza,Vagner Roberto de
Hioka,Noboru
author_role author
author2 Tessaro,André Luiz
Soares,Rafael R. da S.
Souza,Vagner Roberto de
Hioka,Noboru
author2_role author
author
author
author
dc.contributor.author.fl_str_mv Batistela,Vagner Roberto
Tessaro,André Luiz
Soares,Rafael R. da S.
Souza,Vagner Roberto de
Hioka,Noboru
dc.subject.por.fl_str_mv stopped-flow
kinetics
fast reactions
topic stopped-flow
kinetics
fast reactions
description The modern stopped-flow reaction analyzer has shown high efficiency and flexibility, which provides outstanding sample economy with a dead-time of less than 1 ms. However the cost of the equipment imposes a serious restriction to many Brazilian scientists and teachers. In this work we describe the construction of a low-cost stopped-flow system coupled to a UV-Vis spectrophotometer. The performance of the system was checked by monitoring the kinetics of two reactions: the fading of phenolphthalein in aqueous alkaline solution and the chlorophyll a demetallation in acid medium. The apparatus showed reasonable efficiency with a dead-time of 0.3 to 0.5 s. The very good results obtained in these two illustrative processes show that the system is satisfactory for determining rate constants with mean reaction times ranging from seconds to minutes.
publishDate 2006
dc.date.none.fl_str_mv 2006-10-01
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0100-40422006000500034
url http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0100-40422006000500034
dc.language.iso.fl_str_mv por
language por
dc.relation.none.fl_str_mv 10.1590/S0100-40422006000500034
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv text/html
dc.publisher.none.fl_str_mv Sociedade Brasileira de Química
publisher.none.fl_str_mv Sociedade Brasileira de Química
dc.source.none.fl_str_mv Química Nova v.29 n.5 2006
reponame:Química Nova (Online)
instname:Sociedade Brasileira de Química (SBQ)
instacron:SBQ
instname_str Sociedade Brasileira de Química (SBQ)
instacron_str SBQ
institution SBQ
reponame_str Química Nova (Online)
collection Química Nova (Online)
repository.name.fl_str_mv Química Nova (Online) - Sociedade Brasileira de Química (SBQ)
repository.mail.fl_str_mv quimicanova@sbq.org.br
_version_ 1750318105702170624

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