CONSTITUENTS FROM ROOTS OF Maytenus distichophylla, ANTIMICROBIAL ACTIVITY AND TOXICITY FOR CELLS AND Caenorhabditis elegans
Autor(a) principal: | |
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Data de Publicação: | 2020 |
Outros Autores: | , , , , , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Química Nova (Online) |
Texto Completo: | http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0100-40422020000801066 |
Resumo: | Maytenus distichophylla is a medicinal species used in Northeast of Brazil. The hexane (HE), chloroform (CE), ethyl acetate (EAE) and methanol (ME) extracts and compounds from its roots were evaluated for their protective activity against Staphylococcus aureus and Candida albicans. The cytotoxicity for chronic myeloid leukemia (K562), acute monocytic leukemia (THP-1), and peripheral blood mononuclear cells of normal individuals (PBMC) cells was established by MTT method using etoposide as standard. The in vivo toxicity of samples was determined using Caenorhabditis elegans model. From HE and CE were isolated: friedelan-3-one (1), b-sitosterol (2), 3-oxo-olean-9(11),12-diene (3), a mixture of pristimerin (4) and 11a-hydroxylup-20(29)-en-3-one (5), 30-hydroxylup-20(29)-en-3-one (6), friedelane-3,7-dione (7), tingenone (8) and triacylglycerol (9). The structures of 1-9 were confirmed by spectral data. All samples reduced the viability of S. aureus and present no effect against C. albicans. The HE, CE, mixture of 4/5 and 8 reduced 75% of S. aureus viability. Cytotoxic effect for K562 and THP-1 cells was caused by compounds 1, 2 and 8. All samples displayed selectivity for leukemic cells and low toxicity to PBMC cells, suggesting their potential as anticancer agents. Extracts and compounds were non-toxic to L1 larvae of C. elegans. However, most of them reduced significantly young adult worm’s survival, being considered as potential nematicides. |
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CONSTITUENTS FROM ROOTS OF Maytenus distichophylla, ANTIMICROBIAL ACTIVITY AND TOXICITY FOR CELLS AND Caenorhabditis elegansCelastraceaepentacyclic triterpenestoxicityK562 cellsTHP-1 cellsMaytenus distichophylla is a medicinal species used in Northeast of Brazil. The hexane (HE), chloroform (CE), ethyl acetate (EAE) and methanol (ME) extracts and compounds from its roots were evaluated for their protective activity against Staphylococcus aureus and Candida albicans. The cytotoxicity for chronic myeloid leukemia (K562), acute monocytic leukemia (THP-1), and peripheral blood mononuclear cells of normal individuals (PBMC) cells was established by MTT method using etoposide as standard. The in vivo toxicity of samples was determined using Caenorhabditis elegans model. From HE and CE were isolated: friedelan-3-one (1), b-sitosterol (2), 3-oxo-olean-9(11),12-diene (3), a mixture of pristimerin (4) and 11a-hydroxylup-20(29)-en-3-one (5), 30-hydroxylup-20(29)-en-3-one (6), friedelane-3,7-dione (7), tingenone (8) and triacylglycerol (9). The structures of 1-9 were confirmed by spectral data. All samples reduced the viability of S. aureus and present no effect against C. albicans. The HE, CE, mixture of 4/5 and 8 reduced 75% of S. aureus viability. Cytotoxic effect for K562 and THP-1 cells was caused by compounds 1, 2 and 8. All samples displayed selectivity for leukemic cells and low toxicity to PBMC cells, suggesting their potential as anticancer agents. Extracts and compounds were non-toxic to L1 larvae of C. elegans. However, most of them reduced significantly young adult worm’s survival, being considered as potential nematicides.Sociedade Brasileira de Química2020-09-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersiontext/htmlhttp://old.scielo.br/scielo.php?script=sci_arttext&pid=S0100-40422020000801066Química Nova v.43 n.8 2020reponame:Química Nova (Online)instname:Sociedade Brasileira de Química (SBQ)instacron:SBQ10.21577/0100-4042.20170591info:eu-repo/semantics/openAccessMorales,Shirley A. T.Aguilar,Mariana G. dePereira,Rafael C. G.Duarte,Lucienir P.Sousa,Grasiely F.Oliveira,Djalma M. deEvangelista,Fernanda C. G.Sabino,Adriano P.Viana,Roberta O.Alves,Viviane S.Vieira-Filho,Sidney A.eng2020-10-13T00:00:00Zoai:scielo:S0100-40422020000801066Revistahttps://www.scielo.br/j/qn/ONGhttps://old.scielo.br/oai/scielo-oai.phpquimicanova@sbq.org.br1678-70640100-4042opendoar:2020-10-13T00:00Química Nova (Online) - Sociedade Brasileira de Química (SBQ)false |
dc.title.none.fl_str_mv |
CONSTITUENTS FROM ROOTS OF Maytenus distichophylla, ANTIMICROBIAL ACTIVITY AND TOXICITY FOR CELLS AND Caenorhabditis elegans |
title |
CONSTITUENTS FROM ROOTS OF Maytenus distichophylla, ANTIMICROBIAL ACTIVITY AND TOXICITY FOR CELLS AND Caenorhabditis elegans |
spellingShingle |
CONSTITUENTS FROM ROOTS OF Maytenus distichophylla, ANTIMICROBIAL ACTIVITY AND TOXICITY FOR CELLS AND Caenorhabditis elegans Morales,Shirley A. T. Celastraceae pentacyclic triterpenes toxicity K562 cells THP-1 cells |
title_short |
CONSTITUENTS FROM ROOTS OF Maytenus distichophylla, ANTIMICROBIAL ACTIVITY AND TOXICITY FOR CELLS AND Caenorhabditis elegans |
title_full |
CONSTITUENTS FROM ROOTS OF Maytenus distichophylla, ANTIMICROBIAL ACTIVITY AND TOXICITY FOR CELLS AND Caenorhabditis elegans |
title_fullStr |
CONSTITUENTS FROM ROOTS OF Maytenus distichophylla, ANTIMICROBIAL ACTIVITY AND TOXICITY FOR CELLS AND Caenorhabditis elegans |
title_full_unstemmed |
CONSTITUENTS FROM ROOTS OF Maytenus distichophylla, ANTIMICROBIAL ACTIVITY AND TOXICITY FOR CELLS AND Caenorhabditis elegans |
title_sort |
CONSTITUENTS FROM ROOTS OF Maytenus distichophylla, ANTIMICROBIAL ACTIVITY AND TOXICITY FOR CELLS AND Caenorhabditis elegans |
author |
Morales,Shirley A. T. |
author_facet |
Morales,Shirley A. T. Aguilar,Mariana G. de Pereira,Rafael C. G. Duarte,Lucienir P. Sousa,Grasiely F. Oliveira,Djalma M. de Evangelista,Fernanda C. G. Sabino,Adriano P. Viana,Roberta O. Alves,Viviane S. Vieira-Filho,Sidney A. |
author_role |
author |
author2 |
Aguilar,Mariana G. de Pereira,Rafael C. G. Duarte,Lucienir P. Sousa,Grasiely F. Oliveira,Djalma M. de Evangelista,Fernanda C. G. Sabino,Adriano P. Viana,Roberta O. Alves,Viviane S. Vieira-Filho,Sidney A. |
author2_role |
author author author author author author author author author author |
dc.contributor.author.fl_str_mv |
Morales,Shirley A. T. Aguilar,Mariana G. de Pereira,Rafael C. G. Duarte,Lucienir P. Sousa,Grasiely F. Oliveira,Djalma M. de Evangelista,Fernanda C. G. Sabino,Adriano P. Viana,Roberta O. Alves,Viviane S. Vieira-Filho,Sidney A. |
dc.subject.por.fl_str_mv |
Celastraceae pentacyclic triterpenes toxicity K562 cells THP-1 cells |
topic |
Celastraceae pentacyclic triterpenes toxicity K562 cells THP-1 cells |
description |
Maytenus distichophylla is a medicinal species used in Northeast of Brazil. The hexane (HE), chloroform (CE), ethyl acetate (EAE) and methanol (ME) extracts and compounds from its roots were evaluated for their protective activity against Staphylococcus aureus and Candida albicans. The cytotoxicity for chronic myeloid leukemia (K562), acute monocytic leukemia (THP-1), and peripheral blood mononuclear cells of normal individuals (PBMC) cells was established by MTT method using etoposide as standard. The in vivo toxicity of samples was determined using Caenorhabditis elegans model. From HE and CE were isolated: friedelan-3-one (1), b-sitosterol (2), 3-oxo-olean-9(11),12-diene (3), a mixture of pristimerin (4) and 11a-hydroxylup-20(29)-en-3-one (5), 30-hydroxylup-20(29)-en-3-one (6), friedelane-3,7-dione (7), tingenone (8) and triacylglycerol (9). The structures of 1-9 were confirmed by spectral data. All samples reduced the viability of S. aureus and present no effect against C. albicans. The HE, CE, mixture of 4/5 and 8 reduced 75% of S. aureus viability. Cytotoxic effect for K562 and THP-1 cells was caused by compounds 1, 2 and 8. All samples displayed selectivity for leukemic cells and low toxicity to PBMC cells, suggesting their potential as anticancer agents. Extracts and compounds were non-toxic to L1 larvae of C. elegans. However, most of them reduced significantly young adult worm’s survival, being considered as potential nematicides. |
publishDate |
2020 |
dc.date.none.fl_str_mv |
2020-09-01 |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0100-40422020000801066 |
url |
http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0100-40422020000801066 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
10.21577/0100-4042.20170591 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
text/html |
dc.publisher.none.fl_str_mv |
Sociedade Brasileira de Química |
publisher.none.fl_str_mv |
Sociedade Brasileira de Química |
dc.source.none.fl_str_mv |
Química Nova v.43 n.8 2020 reponame:Química Nova (Online) instname:Sociedade Brasileira de Química (SBQ) instacron:SBQ |
instname_str |
Sociedade Brasileira de Química (SBQ) |
instacron_str |
SBQ |
institution |
SBQ |
reponame_str |
Química Nova (Online) |
collection |
Química Nova (Online) |
repository.name.fl_str_mv |
Química Nova (Online) - Sociedade Brasileira de Química (SBQ) |
repository.mail.fl_str_mv |
quimicanova@sbq.org.br |
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1750318120608727040 |