Emprego de metodologia enzimática na obtenção de extratos protéicos de farinha de trigo
Autor(a) principal: | |
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Data de Publicação: | 2009 |
Outros Autores: | , , , , |
Tipo de documento: | Artigo |
Idioma: | por |
Título da fonte: | Química Nova (Online) |
Texto Completo: | http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0100-40422009000400017 |
Resumo: | An enzymatic method was used for obtaining protein extracts from wheat flour using an alkaline protease. Some parameters were evaluated aiming the optimization of this method: temperature (40-50 ºC); time (2-5 h); physical treatment of the sample (no treatment, ultra-turrax/16,000 rpm/5 min and ultrasound/120 W/10 min); enzyme:substrate ratio (E:S) of 5:100 - 10:100 and concentration of wheat flour (1:3, 1:5 and 1:10 w/v). The results showed that the best condition for protein extraction was that using the sample concentration of 1:3 (w/v), ultra-turrax, E:S of 10:100, at 40 ºC, 2 h, having reached an extraction yield of 88.53%. |
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Emprego de metodologia enzimática na obtenção de extratos protéicos de farinha de trigocerealwheat flourprotein extractionAn enzymatic method was used for obtaining protein extracts from wheat flour using an alkaline protease. Some parameters were evaluated aiming the optimization of this method: temperature (40-50 ºC); time (2-5 h); physical treatment of the sample (no treatment, ultra-turrax/16,000 rpm/5 min and ultrasound/120 W/10 min); enzyme:substrate ratio (E:S) of 5:100 - 10:100 and concentration of wheat flour (1:3, 1:5 and 1:10 w/v). The results showed that the best condition for protein extraction was that using the sample concentration of 1:3 (w/v), ultra-turrax, E:S of 10:100, at 40 ºC, 2 h, having reached an extraction yield of 88.53%.Sociedade Brasileira de Química2009-01-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersiontext/htmlhttp://old.scielo.br/scielo.php?script=sci_arttext&pid=S0100-40422009000400017Química Nova v.32 n.4 2009reponame:Química Nova (Online)instname:Sociedade Brasileira de Química (SBQ)instacron:SBQ10.1590/S0100-40422009000400017info:eu-repo/semantics/openAccessCarreira,Raquel LinharesRamos,Camila SallesSilva,Mauro RamalhoLopes Junior,Carlos de OliveiraSilvestre,Marialice Pinto CoelhoPirozi,Mônica Ribeiropor2009-06-09T00:00:00Zoai:scielo:S0100-40422009000400017Revistahttps://www.scielo.br/j/qn/ONGhttps://old.scielo.br/oai/scielo-oai.phpquimicanova@sbq.org.br1678-70640100-4042opendoar:2009-06-09T00:00Química Nova (Online) - Sociedade Brasileira de Química (SBQ)false |
dc.title.none.fl_str_mv |
Emprego de metodologia enzimática na obtenção de extratos protéicos de farinha de trigo |
title |
Emprego de metodologia enzimática na obtenção de extratos protéicos de farinha de trigo |
spellingShingle |
Emprego de metodologia enzimática na obtenção de extratos protéicos de farinha de trigo Carreira,Raquel Linhares cereal wheat flour protein extraction |
title_short |
Emprego de metodologia enzimática na obtenção de extratos protéicos de farinha de trigo |
title_full |
Emprego de metodologia enzimática na obtenção de extratos protéicos de farinha de trigo |
title_fullStr |
Emprego de metodologia enzimática na obtenção de extratos protéicos de farinha de trigo |
title_full_unstemmed |
Emprego de metodologia enzimática na obtenção de extratos protéicos de farinha de trigo |
title_sort |
Emprego de metodologia enzimática na obtenção de extratos protéicos de farinha de trigo |
author |
Carreira,Raquel Linhares |
author_facet |
Carreira,Raquel Linhares Ramos,Camila Salles Silva,Mauro Ramalho Lopes Junior,Carlos de Oliveira Silvestre,Marialice Pinto Coelho Pirozi,Mônica Ribeiro |
author_role |
author |
author2 |
Ramos,Camila Salles Silva,Mauro Ramalho Lopes Junior,Carlos de Oliveira Silvestre,Marialice Pinto Coelho Pirozi,Mônica Ribeiro |
author2_role |
author author author author author |
dc.contributor.author.fl_str_mv |
Carreira,Raquel Linhares Ramos,Camila Salles Silva,Mauro Ramalho Lopes Junior,Carlos de Oliveira Silvestre,Marialice Pinto Coelho Pirozi,Mônica Ribeiro |
dc.subject.por.fl_str_mv |
cereal wheat flour protein extraction |
topic |
cereal wheat flour protein extraction |
description |
An enzymatic method was used for obtaining protein extracts from wheat flour using an alkaline protease. Some parameters were evaluated aiming the optimization of this method: temperature (40-50 ºC); time (2-5 h); physical treatment of the sample (no treatment, ultra-turrax/16,000 rpm/5 min and ultrasound/120 W/10 min); enzyme:substrate ratio (E:S) of 5:100 - 10:100 and concentration of wheat flour (1:3, 1:5 and 1:10 w/v). The results showed that the best condition for protein extraction was that using the sample concentration of 1:3 (w/v), ultra-turrax, E:S of 10:100, at 40 ºC, 2 h, having reached an extraction yield of 88.53%. |
publishDate |
2009 |
dc.date.none.fl_str_mv |
2009-01-01 |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0100-40422009000400017 |
url |
http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0100-40422009000400017 |
dc.language.iso.fl_str_mv |
por |
language |
por |
dc.relation.none.fl_str_mv |
10.1590/S0100-40422009000400017 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
text/html |
dc.publisher.none.fl_str_mv |
Sociedade Brasileira de Química |
publisher.none.fl_str_mv |
Sociedade Brasileira de Química |
dc.source.none.fl_str_mv |
Química Nova v.32 n.4 2009 reponame:Química Nova (Online) instname:Sociedade Brasileira de Química (SBQ) instacron:SBQ |
instname_str |
Sociedade Brasileira de Química (SBQ) |
instacron_str |
SBQ |
institution |
SBQ |
reponame_str |
Química Nova (Online) |
collection |
Química Nova (Online) |
repository.name.fl_str_mv |
Química Nova (Online) - Sociedade Brasileira de Química (SBQ) |
repository.mail.fl_str_mv |
quimicanova@sbq.org.br |
_version_ |
1750318109090119680 |