Reproductive biology and cytology of Hypericum brasiliense Choisy (Hypericaceae)
Autor(a) principal: | |
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Data de Publicação: | 2009 |
Outros Autores: | , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Brazilian Journal of Botany |
Texto Completo: | http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0100-84042009000300013 |
Resumo: | This is the first study of reproductive biology and cytology carried out with Hypericum brasiliense, a species with medicinal properties and potential agronomic interest. Three populations of H. brasiliense collected at Southeastern Brazil were studied. The results indicate that H. brasiliense is preferentially allogamous, self-compatible, facultative apomitic and anemophilous. Male sterility was observed in about 50% of individuals from the three populations. Anatomical studies evidenced structural abnormalities in anthers of male sterile flowers, showing enlarged tapetal cells and thick secretion deposits on the tapetal cell surfaces that may cause nutritional deficit for pollen mother cells. In cytogenetic studies several haploid chromosome numbers were observed like n = 4, 8, 9, 11, 16 and 17, including the presence of multivalents and micronuclei in tetrads, indicating the occurrence of abnormalities in the meiotic process of H. brasiliense. Despite these meiotic abnormalities the pollen viability and in vitro pollen germination rate observed in fertile flowers may be considered high. The diploid chromosome number 2n = 16 was observed, and the chromosomes in metaphase were small and similar. Fluorochrome staining techniques using DAPI and CMA3 were applied, with no positive bands observed. |
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Reproductive biology and cytology of Hypericum brasiliense Choisy (Hypericaceae)anatomy of anthersapomixyirregular meiosismale sterilityThis is the first study of reproductive biology and cytology carried out with Hypericum brasiliense, a species with medicinal properties and potential agronomic interest. Three populations of H. brasiliense collected at Southeastern Brazil were studied. The results indicate that H. brasiliense is preferentially allogamous, self-compatible, facultative apomitic and anemophilous. Male sterility was observed in about 50% of individuals from the three populations. Anatomical studies evidenced structural abnormalities in anthers of male sterile flowers, showing enlarged tapetal cells and thick secretion deposits on the tapetal cell surfaces that may cause nutritional deficit for pollen mother cells. In cytogenetic studies several haploid chromosome numbers were observed like n = 4, 8, 9, 11, 16 and 17, including the presence of multivalents and micronuclei in tetrads, indicating the occurrence of abnormalities in the meiotic process of H. brasiliense. Despite these meiotic abnormalities the pollen viability and in vitro pollen germination rate observed in fertile flowers may be considered high. The diploid chromosome number 2n = 16 was observed, and the chromosomes in metaphase were small and similar. Fluorochrome staining techniques using DAPI and CMA3 were applied, with no positive bands observed.Sociedade Botânica de São Paulo2009-09-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersiontext/htmlhttp://old.scielo.br/scielo.php?script=sci_arttext&pid=S0100-84042009000300013Brazilian Journal of Botany v.32 n.3 2009reponame:Brazilian Journal of Botanyinstname:Sociedade Botânica de São Paulo (SBSP)instacron:SBSP10.1590/S0100-84042009000300013info:eu-repo/semantics/openAccessMoraes,Izabel Cristina Ribeiro dePinto-Maglio,Cecília Alzira FerreiraLombello,Ricardo Augustoeng2009-12-07T00:00:00Zoai:scielo:S0100-84042009000300013Revistahttps://www.scielo.br/j/rbb/ONGhttps://old.scielo.br/oai/scielo-oai.phpbrazbot@gmail.com||brazbot@gmail.com1806-99590100-8404opendoar:2009-12-07T00:00Brazilian Journal of Botany - Sociedade Botânica de São Paulo (SBSP)false |
dc.title.none.fl_str_mv |
Reproductive biology and cytology of Hypericum brasiliense Choisy (Hypericaceae) |
title |
Reproductive biology and cytology of Hypericum brasiliense Choisy (Hypericaceae) |
spellingShingle |
Reproductive biology and cytology of Hypericum brasiliense Choisy (Hypericaceae) Moraes,Izabel Cristina Ribeiro de anatomy of anthers apomixy irregular meiosis male sterility |
title_short |
Reproductive biology and cytology of Hypericum brasiliense Choisy (Hypericaceae) |
title_full |
Reproductive biology and cytology of Hypericum brasiliense Choisy (Hypericaceae) |
title_fullStr |
Reproductive biology and cytology of Hypericum brasiliense Choisy (Hypericaceae) |
title_full_unstemmed |
Reproductive biology and cytology of Hypericum brasiliense Choisy (Hypericaceae) |
title_sort |
Reproductive biology and cytology of Hypericum brasiliense Choisy (Hypericaceae) |
author |
Moraes,Izabel Cristina Ribeiro de |
author_facet |
Moraes,Izabel Cristina Ribeiro de Pinto-Maglio,Cecília Alzira Ferreira Lombello,Ricardo Augusto |
author_role |
author |
author2 |
Pinto-Maglio,Cecília Alzira Ferreira Lombello,Ricardo Augusto |
author2_role |
author author |
dc.contributor.author.fl_str_mv |
Moraes,Izabel Cristina Ribeiro de Pinto-Maglio,Cecília Alzira Ferreira Lombello,Ricardo Augusto |
dc.subject.por.fl_str_mv |
anatomy of anthers apomixy irregular meiosis male sterility |
topic |
anatomy of anthers apomixy irregular meiosis male sterility |
description |
This is the first study of reproductive biology and cytology carried out with Hypericum brasiliense, a species with medicinal properties and potential agronomic interest. Three populations of H. brasiliense collected at Southeastern Brazil were studied. The results indicate that H. brasiliense is preferentially allogamous, self-compatible, facultative apomitic and anemophilous. Male sterility was observed in about 50% of individuals from the three populations. Anatomical studies evidenced structural abnormalities in anthers of male sterile flowers, showing enlarged tapetal cells and thick secretion deposits on the tapetal cell surfaces that may cause nutritional deficit for pollen mother cells. In cytogenetic studies several haploid chromosome numbers were observed like n = 4, 8, 9, 11, 16 and 17, including the presence of multivalents and micronuclei in tetrads, indicating the occurrence of abnormalities in the meiotic process of H. brasiliense. Despite these meiotic abnormalities the pollen viability and in vitro pollen germination rate observed in fertile flowers may be considered high. The diploid chromosome number 2n = 16 was observed, and the chromosomes in metaphase were small and similar. Fluorochrome staining techniques using DAPI and CMA3 were applied, with no positive bands observed. |
publishDate |
2009 |
dc.date.none.fl_str_mv |
2009-09-01 |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0100-84042009000300013 |
url |
http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0100-84042009000300013 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
10.1590/S0100-84042009000300013 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
text/html |
dc.publisher.none.fl_str_mv |
Sociedade Botânica de São Paulo |
publisher.none.fl_str_mv |
Sociedade Botânica de São Paulo |
dc.source.none.fl_str_mv |
Brazilian Journal of Botany v.32 n.3 2009 reponame:Brazilian Journal of Botany instname:Sociedade Botânica de São Paulo (SBSP) instacron:SBSP |
instname_str |
Sociedade Botânica de São Paulo (SBSP) |
instacron_str |
SBSP |
institution |
SBSP |
reponame_str |
Brazilian Journal of Botany |
collection |
Brazilian Journal of Botany |
repository.name.fl_str_mv |
Brazilian Journal of Botany - Sociedade Botânica de São Paulo (SBSP) |
repository.mail.fl_str_mv |
brazbot@gmail.com||brazbot@gmail.com |
_version_ |
1754734840171724800 |