Synthesis and characterization of Biosilicate/F18 bioglass scaffolds for dental application
Autor(a) principal: | |
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Data de Publicação: | 2019 |
Tipo de documento: | Tese |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UFSCAR |
Texto Completo: | https://repositorio.ufscar.br/handle/ufscar/16870 |
Resumo: | In this work, Biosilicate scaffolds were synthesized using the foam replica technique. Biosilicate is highly bioactive, biodegradable, antibacterial, and non-toxic. Despite of these properties, Biosilicate scaffolds present low mechanical strength, limiting their clinical applications. Therefore, our aim was to increase the mechanical properties of the Biosilicate scaffolds by several F18 glass coatings. First, the Ryshkewitch and Ashby & Gibson models were used to calculate the maximum theoretical compressive strength of the scaffolds in function of their porosity, taking into account the ideal conditions found in the literature. Biosilicate scaffolds were prepared through the foam replica technique; then, they were coated several times with F18 Bioglass slurry to eliminate their defects and reinforce their structures. The scaffolds were characterized by microstructure, total porosity, average cell size, and compressive strength. The material exhibited a total porosity of 82%, an average cell size of 525 μm, and compressive strength of 3.3 (± 0.3) MPa, values in the range of commercial scaffolds based on Hydroxiapatite and β-TCP. Scanning Electron Microscopy showed that F18 bioglass helped to remove surface defects and partially infiltrated the hollow Biosilicate-struts, increasing significantly the resistance of the material. Also, in vitro osteogenic differentiation of human Adipose-derived mesenchymal Stem Cells (hASCs) was evaluated using F18 glass-coated Biosilicate scaffolds and their ionic dissolution products. Gene expression profiles of cells were evaluated using the RT2 Profiler PCR microarray on day 21. Mineralizing tissue-associated proteins and osteogenic differentiation factor expressions were measured using Q-PCR. Additionally, alkaline phosphatase enzyme production and extracellular matrix mineralization were evaluated. The alkaline phosphatase activity, mineralization and bone-related gene expression of hASCs were significantly enhanced upon stimulation with both scaffolds and their ionic extracts. This work evidenced that F18 glass-coated Biosilicate scaffolds have a high potential for dental applications. |
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Abadia, Claudia Patricia MarinZanotto, Edgar Dutrahttp://lattes.cnpq.br/1055167132036400Crovace, Murilo Camurihttp://lattes.cnpq.br/2960564171443068http://lattes.cnpq.br/34410401545259612e600f39-a378-4758-85fe-f22e5c5d1ebe2022-10-14T11:30:28Z2022-10-14T11:30:28Z2019-10-16ABADIA, Claudia Patricia Marin. Synthesis and characterization of Biosilicate/F18 bioglass scaffolds for dental application. 2019. Tese (Doutorado em Ciência e Engenharia de Materiais) – Universidade Federal de São Carlos, São Carlos, 2019. Disponível em: https://repositorio.ufscar.br/handle/ufscar/16870.https://repositorio.ufscar.br/handle/ufscar/16870In this work, Biosilicate scaffolds were synthesized using the foam replica technique. Biosilicate is highly bioactive, biodegradable, antibacterial, and non-toxic. Despite of these properties, Biosilicate scaffolds present low mechanical strength, limiting their clinical applications. Therefore, our aim was to increase the mechanical properties of the Biosilicate scaffolds by several F18 glass coatings. First, the Ryshkewitch and Ashby & Gibson models were used to calculate the maximum theoretical compressive strength of the scaffolds in function of their porosity, taking into account the ideal conditions found in the literature. Biosilicate scaffolds were prepared through the foam replica technique; then, they were coated several times with F18 Bioglass slurry to eliminate their defects and reinforce their structures. The scaffolds were characterized by microstructure, total porosity, average cell size, and compressive strength. The material exhibited a total porosity of 82%, an average cell size of 525 μm, and compressive strength of 3.3 (± 0.3) MPa, values in the range of commercial scaffolds based on Hydroxiapatite and β-TCP. Scanning Electron Microscopy showed that F18 bioglass helped to remove surface defects and partially infiltrated the hollow Biosilicate-struts, increasing significantly the resistance of the material. Also, in vitro osteogenic differentiation of human Adipose-derived mesenchymal Stem Cells (hASCs) was evaluated using F18 glass-coated Biosilicate scaffolds and their ionic dissolution products. Gene expression profiles of cells were evaluated using the RT2 Profiler PCR microarray on day 21. Mineralizing tissue-associated proteins and osteogenic differentiation factor expressions were measured using Q-PCR. Additionally, alkaline phosphatase enzyme production and extracellular matrix mineralization were evaluated. The alkaline phosphatase activity, mineralization and bone-related gene expression of hASCs were significantly enhanced upon stimulation with both scaffolds and their ionic extracts. This work evidenced that F18 glass-coated Biosilicate scaffolds have a high potential for dental applications.O Biovidro F18 e o Biosilicato são biomateriais com uma alta bioatividade. No entanto, os scaffolds de Biosilicato apresentam baixa resistência mecânica, o que impede sua aplicação clínica. Por essa razão, nosso interesse foi desenvolver scaffolds combinando o Biosilicato e o F18 para incrementar as propriedades mecânicas do material. Inicialmente, empregando-se os modelos de Ryshkewitch e de Ashby & Gibson, foi calculada a resistência mecânica teórica máxima de um scaffold. Os scaffolds de Biosilicato foram preparados através da técnica de réplica e, em seguida, foram recobertos várias vezes com uma suspensão de F18, de forma a eliminar defeitos e, portanto, reforçar a sua estrutura. Os scaffolds obtidos foram caracterizados em relação à microestrutura, porosidade total, abertura média das células e resistência mecânica à compressão. Os resultados mostraram que os scaffolds apresentam uma porosidade total de 82%, com abertura média de células de 525 μm e resistência mecânica à compressão de 3,3 MPa, valores compatíveis com os scaffolds comerciais à base de hidroxiapatita ou β-TCP. As análises de Microscopia Eletrônica de Varredura mostraram que o F18 ajudou a eliminar defeitos superficiais e infiltrou-se parcialmente na estrutura oca dos scaffolds, aumentando significativamente sua resistência mecânica. A diferenciação osteogênica in vitro de células-tronco mesenquimais foi avaliada usando os scaffolds de Biosilicato recobertos com Biovidrio, assim como os íons liberados pelo biomaterial. A expressão gênica foi avaliada utilizando a metodologia de PCR após 21 dias em meio osteogênico. A expressão de fatores associados à diferenciação osteogênica foram medidas usando Q-PCR. Além disso, avaliou-se a produção da enzima fosfatase alcalina e a mineralização. A atividade de diversos fatores foi significativamente aumentada na presença dos scaffolds ou de seus produtos de dissolução. Estes resultados mostram que os scaffolds desenvolvidos neste trabalho possuem grande potencial para aplicações em odontologia.Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)engUniversidade Federal de São CarlosCâmpus São CarlosPrograma de Pós-Graduação em Ciência e Engenharia de Materiais - PPGCEMUFSCarAttribution-NonCommercial-NoDerivs 3.0 Brazilhttp://creativecommons.org/licenses/by-nc-nd/3.0/br/info:eu-repo/semantics/openAccessBiosilicatoBiovidro F18RecobrimentoDiferenciação osteogênicaBiosilicateF18 glassScaffoldCoatingOsteogenic DifferentiationENGENHARIAS::ENGENHARIA DE MATERIAIS E METALURGICASynthesis and characterization of Biosilicate/F18 bioglass scaffolds for dental applicationSíntese e caracterização de andaimes de Biosilicate/F18 biovidro para aplicação odontológicainfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/doctoralThesis6006006cfabc63-f3c2-48d1-ba85-c4d9edda486breponame:Repositório Institucional da UFSCARinstname:Universidade Federal de São Carlos (UFSCAR)instacron:UFSCARORIGINALClaudia Patricia Marin Abadia - Tese .pdfClaudia Patricia Marin Abadia - Tese .pdfapplication/pdf8480975https://repositorio.ufscar.br/bitstream/ufscar/16870/1/Claudia%20Patricia%20Marin%20Abadia%20-%20Tese%20.pdf2aa0e75267883fb7451252672b6aeb4bMD51BCO carta comprovante autoarquivamento P.pdfBCO carta comprovante autoarquivamento P.pdfapplication/pdf1238886https://repositorio.ufscar.br/bitstream/ufscar/16870/2/BCO%20carta%20comprovante%20autoarquivamento%20P.pdfd9ff1da07a12b9528c8b99527a6e5e6aMD52CC-LICENSElicense_rdflicense_rdfapplication/rdf+xml; charset=utf-8811https://repositorio.ufscar.br/bitstream/ufscar/16870/3/license_rdfe39d27027a6cc9cb039ad269a5db8e34MD53TEXTClaudia Patricia Marin Abadia - Tese .pdf.txtClaudia Patricia Marin Abadia - Tese .pdf.txtExtracted texttext/plain305369https://repositorio.ufscar.br/bitstream/ufscar/16870/4/Claudia%20Patricia%20Marin%20Abadia%20-%20Tese%20.pdf.txta9cb51653a6b7ff02cdcf4eb87a9858dMD54BCO carta comprovante autoarquivamento P.pdf.txtBCO carta comprovante autoarquivamento P.pdf.txtExtracted texttext/plain1https://repositorio.ufscar.br/bitstream/ufscar/16870/6/BCO%20carta%20comprovante%20autoarquivamento%20P.pdf.txt68b329da9893e34099c7d8ad5cb9c940MD56THUMBNAILClaudia Patricia Marin Abadia - Tese .pdf.jpgClaudia Patricia Marin Abadia - Tese .pdf.jpgIM Thumbnailimage/jpeg6009https://repositorio.ufscar.br/bitstream/ufscar/16870/5/Claudia%20Patricia%20Marin%20Abadia%20-%20Tese%20.pdf.jpg132b5ed764b5de410d3a55a812561fb6MD55BCO carta comprovante autoarquivamento P.pdf.jpgBCO carta comprovante autoarquivamento P.pdf.jpgIM Thumbnailimage/jpeg14157https://repositorio.ufscar.br/bitstream/ufscar/16870/7/BCO%20carta%20comprovante%20autoarquivamento%20P.pdf.jpgce5046946be35402ed955ef6c9bb0106MD57ufscar/168702023-09-18 18:32:33.115oai:repositorio.ufscar.br:ufscar/16870Repositório InstitucionalPUBhttps://repositorio.ufscar.br/oai/requestopendoar:43222023-09-18T18:32:33Repositório Institucional da UFSCAR - Universidade Federal de São Carlos (UFSCAR)false |
dc.title.eng.fl_str_mv |
Synthesis and characterization of Biosilicate/F18 bioglass scaffolds for dental application |
dc.title.alternative.por.fl_str_mv |
Síntese e caracterização de andaimes de Biosilicate/F18 biovidro para aplicação odontológica |
title |
Synthesis and characterization of Biosilicate/F18 bioglass scaffolds for dental application |
spellingShingle |
Synthesis and characterization of Biosilicate/F18 bioglass scaffolds for dental application Abadia, Claudia Patricia Marin Biosilicato Biovidro F18 Recobrimento Diferenciação osteogênica Biosilicate F18 glass Scaffold Coating Osteogenic Differentiation ENGENHARIAS::ENGENHARIA DE MATERIAIS E METALURGICA |
title_short |
Synthesis and characterization of Biosilicate/F18 bioglass scaffolds for dental application |
title_full |
Synthesis and characterization of Biosilicate/F18 bioglass scaffolds for dental application |
title_fullStr |
Synthesis and characterization of Biosilicate/F18 bioglass scaffolds for dental application |
title_full_unstemmed |
Synthesis and characterization of Biosilicate/F18 bioglass scaffolds for dental application |
title_sort |
Synthesis and characterization of Biosilicate/F18 bioglass scaffolds for dental application |
author |
Abadia, Claudia Patricia Marin |
author_facet |
Abadia, Claudia Patricia Marin |
author_role |
author |
dc.contributor.authorlattes.por.fl_str_mv |
http://lattes.cnpq.br/3441040154525961 |
dc.contributor.author.fl_str_mv |
Abadia, Claudia Patricia Marin |
dc.contributor.advisor1.fl_str_mv |
Zanotto, Edgar Dutra |
dc.contributor.advisor1Lattes.fl_str_mv |
http://lattes.cnpq.br/1055167132036400 |
dc.contributor.advisor-co1.fl_str_mv |
Crovace, Murilo Camuri |
dc.contributor.advisor-co1Lattes.fl_str_mv |
http://lattes.cnpq.br/2960564171443068 |
dc.contributor.authorID.fl_str_mv |
2e600f39-a378-4758-85fe-f22e5c5d1ebe |
contributor_str_mv |
Zanotto, Edgar Dutra Crovace, Murilo Camuri |
dc.subject.por.fl_str_mv |
Biosilicato Biovidro F18 Recobrimento Diferenciação osteogênica |
topic |
Biosilicato Biovidro F18 Recobrimento Diferenciação osteogênica Biosilicate F18 glass Scaffold Coating Osteogenic Differentiation ENGENHARIAS::ENGENHARIA DE MATERIAIS E METALURGICA |
dc.subject.eng.fl_str_mv |
Biosilicate F18 glass Scaffold Coating Osteogenic Differentiation |
dc.subject.cnpq.fl_str_mv |
ENGENHARIAS::ENGENHARIA DE MATERIAIS E METALURGICA |
description |
In this work, Biosilicate scaffolds were synthesized using the foam replica technique. Biosilicate is highly bioactive, biodegradable, antibacterial, and non-toxic. Despite of these properties, Biosilicate scaffolds present low mechanical strength, limiting their clinical applications. Therefore, our aim was to increase the mechanical properties of the Biosilicate scaffolds by several F18 glass coatings. First, the Ryshkewitch and Ashby & Gibson models were used to calculate the maximum theoretical compressive strength of the scaffolds in function of their porosity, taking into account the ideal conditions found in the literature. Biosilicate scaffolds were prepared through the foam replica technique; then, they were coated several times with F18 Bioglass slurry to eliminate their defects and reinforce their structures. The scaffolds were characterized by microstructure, total porosity, average cell size, and compressive strength. The material exhibited a total porosity of 82%, an average cell size of 525 μm, and compressive strength of 3.3 (± 0.3) MPa, values in the range of commercial scaffolds based on Hydroxiapatite and β-TCP. Scanning Electron Microscopy showed that F18 bioglass helped to remove surface defects and partially infiltrated the hollow Biosilicate-struts, increasing significantly the resistance of the material. Also, in vitro osteogenic differentiation of human Adipose-derived mesenchymal Stem Cells (hASCs) was evaluated using F18 glass-coated Biosilicate scaffolds and their ionic dissolution products. Gene expression profiles of cells were evaluated using the RT2 Profiler PCR microarray on day 21. Mineralizing tissue-associated proteins and osteogenic differentiation factor expressions were measured using Q-PCR. Additionally, alkaline phosphatase enzyme production and extracellular matrix mineralization were evaluated. The alkaline phosphatase activity, mineralization and bone-related gene expression of hASCs were significantly enhanced upon stimulation with both scaffolds and their ionic extracts. This work evidenced that F18 glass-coated Biosilicate scaffolds have a high potential for dental applications. |
publishDate |
2019 |
dc.date.issued.fl_str_mv |
2019-10-16 |
dc.date.accessioned.fl_str_mv |
2022-10-14T11:30:28Z |
dc.date.available.fl_str_mv |
2022-10-14T11:30:28Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/doctoralThesis |
format |
doctoralThesis |
status_str |
publishedVersion |
dc.identifier.citation.fl_str_mv |
ABADIA, Claudia Patricia Marin. Synthesis and characterization of Biosilicate/F18 bioglass scaffolds for dental application. 2019. Tese (Doutorado em Ciência e Engenharia de Materiais) – Universidade Federal de São Carlos, São Carlos, 2019. Disponível em: https://repositorio.ufscar.br/handle/ufscar/16870. |
dc.identifier.uri.fl_str_mv |
https://repositorio.ufscar.br/handle/ufscar/16870 |
identifier_str_mv |
ABADIA, Claudia Patricia Marin. Synthesis and characterization of Biosilicate/F18 bioglass scaffolds for dental application. 2019. Tese (Doutorado em Ciência e Engenharia de Materiais) – Universidade Federal de São Carlos, São Carlos, 2019. Disponível em: https://repositorio.ufscar.br/handle/ufscar/16870. |
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https://repositorio.ufscar.br/handle/ufscar/16870 |
dc.language.iso.fl_str_mv |
eng |
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eng |
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600 600 |
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6cfabc63-f3c2-48d1-ba85-c4d9edda486b |
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Attribution-NonCommercial-NoDerivs 3.0 Brazil http://creativecommons.org/licenses/by-nc-nd/3.0/br/ info:eu-repo/semantics/openAccess |
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Attribution-NonCommercial-NoDerivs 3.0 Brazil http://creativecommons.org/licenses/by-nc-nd/3.0/br/ |
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openAccess |
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Universidade Federal de São Carlos Câmpus São Carlos |
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Programa de Pós-Graduação em Ciência e Engenharia de Materiais - PPGCEM |
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UFSCar |
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Universidade Federal de São Carlos Câmpus São Carlos |
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