Anatomical Characterization of the Adventitious Roots of Mate (Ilex paraguariensis A. St.-Hil.) Mini-cuttings

Detalhes bibliográficos
Autor(a) principal: Pimentel,Nathalia
Data de Publicação: 2020
Outros Autores: Pedroso,Marina Favarin, Lencina,Kelen Haygert, Oliveira,João Marcelo Santos de, Bisognin,Dilson Antônio
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Brazilian Archives of Biology and Technology
Texto Completo: http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132020000100407
Resumo: Abstract Leaves of mate is one of the main non-timber forest products marketed in South America, which makes establishment of new plantations of great interest. However, vegetative propagation of mate plantlets presents difficulties, which may be associated with the complexity of adventitious root formation. The aims of this study were to anatomically characterize the adventitious roots of mate-clone mini-cuttings and investigate the relationship of phenols and starch with adventitious rooting competence in mini-cuttings treated or not with indole-butyric acid (IBA). The mini-cuttings of four clones were collected at 0, 30, and 60 days of cultivation, fixed in a solution containing 1% glutaraldehyde and 4% formaldehyde, pre-infiltrated and infiltrated in (2-hydroxyethyl) methacrylate, and sectioned in a microtome. Ferric chloride and toluidine blue were used to detect phenolic compounds and lugol to identify starch. Adventitious roots formed in mini-cuttings treated with IBA presented disorganized xylem and phloem and poles irregularly but exhibited sclerenchyma vessel elements and tracheid cells indicating functionality. Differences in the rhizogenic ability of mate clones mini-cuttings were not due to the presence of anatomical barriers or the accumulation of phenolic compounds but be associated with the presence and distribution of starch grains in vegetative propagules.
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spelling Anatomical Characterization of the Adventitious Roots of Mate (Ilex paraguariensis A. St.-Hil.) Mini-cuttingsvegetative propagationindole-butyric acidanatomical barrierphenolicsstarch Abstract Leaves of mate is one of the main non-timber forest products marketed in South America, which makes establishment of new plantations of great interest. However, vegetative propagation of mate plantlets presents difficulties, which may be associated with the complexity of adventitious root formation. The aims of this study were to anatomically characterize the adventitious roots of mate-clone mini-cuttings and investigate the relationship of phenols and starch with adventitious rooting competence in mini-cuttings treated or not with indole-butyric acid (IBA). The mini-cuttings of four clones were collected at 0, 30, and 60 days of cultivation, fixed in a solution containing 1% glutaraldehyde and 4% formaldehyde, pre-infiltrated and infiltrated in (2-hydroxyethyl) methacrylate, and sectioned in a microtome. Ferric chloride and toluidine blue were used to detect phenolic compounds and lugol to identify starch. Adventitious roots formed in mini-cuttings treated with IBA presented disorganized xylem and phloem and poles irregularly but exhibited sclerenchyma vessel elements and tracheid cells indicating functionality. Differences in the rhizogenic ability of mate clones mini-cuttings were not due to the presence of anatomical barriers or the accumulation of phenolic compounds but be associated with the presence and distribution of starch grains in vegetative propagules.Instituto de Tecnologia do Paraná - Tecpar2020-01-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersiontext/htmlhttp://old.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132020000100407Brazilian Archives of Biology and Technology v.63 2020reponame:Brazilian Archives of Biology and Technologyinstname:Instituto de Tecnologia do Paraná (Tecpar)instacron:TECPAR10.1590/1678-4324-2020190359info:eu-repo/semantics/openAccessPimentel,NathaliaPedroso,Marina FavarinLencina,Kelen HaygertOliveira,João Marcelo Santos deBisognin,Dilson Antônioeng2020-05-04T00:00:00Zoai:scielo:S1516-89132020000100407Revistahttps://www.scielo.br/j/babt/https://old.scielo.br/oai/scielo-oai.phpbabt@tecpar.br||babt@tecpar.br1678-43241516-8913opendoar:2020-05-04T00:00Brazilian Archives of Biology and Technology - Instituto de Tecnologia do Paraná (Tecpar)false
dc.title.none.fl_str_mv Anatomical Characterization of the Adventitious Roots of Mate (Ilex paraguariensis A. St.-Hil.) Mini-cuttings
title Anatomical Characterization of the Adventitious Roots of Mate (Ilex paraguariensis A. St.-Hil.) Mini-cuttings
spellingShingle Anatomical Characterization of the Adventitious Roots of Mate (Ilex paraguariensis A. St.-Hil.) Mini-cuttings
Pimentel,Nathalia
vegetative propagation
indole-butyric acid
anatomical barrier
phenolics
starch
title_short Anatomical Characterization of the Adventitious Roots of Mate (Ilex paraguariensis A. St.-Hil.) Mini-cuttings
title_full Anatomical Characterization of the Adventitious Roots of Mate (Ilex paraguariensis A. St.-Hil.) Mini-cuttings
title_fullStr Anatomical Characterization of the Adventitious Roots of Mate (Ilex paraguariensis A. St.-Hil.) Mini-cuttings
title_full_unstemmed Anatomical Characterization of the Adventitious Roots of Mate (Ilex paraguariensis A. St.-Hil.) Mini-cuttings
title_sort Anatomical Characterization of the Adventitious Roots of Mate (Ilex paraguariensis A. St.-Hil.) Mini-cuttings
author Pimentel,Nathalia
author_facet Pimentel,Nathalia
Pedroso,Marina Favarin
Lencina,Kelen Haygert
Oliveira,João Marcelo Santos de
Bisognin,Dilson Antônio
author_role author
author2 Pedroso,Marina Favarin
Lencina,Kelen Haygert
Oliveira,João Marcelo Santos de
Bisognin,Dilson Antônio
author2_role author
author
author
author
dc.contributor.author.fl_str_mv Pimentel,Nathalia
Pedroso,Marina Favarin
Lencina,Kelen Haygert
Oliveira,João Marcelo Santos de
Bisognin,Dilson Antônio
dc.subject.por.fl_str_mv vegetative propagation
indole-butyric acid
anatomical barrier
phenolics
starch
topic vegetative propagation
indole-butyric acid
anatomical barrier
phenolics
starch
description Abstract Leaves of mate is one of the main non-timber forest products marketed in South America, which makes establishment of new plantations of great interest. However, vegetative propagation of mate plantlets presents difficulties, which may be associated with the complexity of adventitious root formation. The aims of this study were to anatomically characterize the adventitious roots of mate-clone mini-cuttings and investigate the relationship of phenols and starch with adventitious rooting competence in mini-cuttings treated or not with indole-butyric acid (IBA). The mini-cuttings of four clones were collected at 0, 30, and 60 days of cultivation, fixed in a solution containing 1% glutaraldehyde and 4% formaldehyde, pre-infiltrated and infiltrated in (2-hydroxyethyl) methacrylate, and sectioned in a microtome. Ferric chloride and toluidine blue were used to detect phenolic compounds and lugol to identify starch. Adventitious roots formed in mini-cuttings treated with IBA presented disorganized xylem and phloem and poles irregularly but exhibited sclerenchyma vessel elements and tracheid cells indicating functionality. Differences in the rhizogenic ability of mate clones mini-cuttings were not due to the presence of anatomical barriers or the accumulation of phenolic compounds but be associated with the presence and distribution of starch grains in vegetative propagules.
publishDate 2020
dc.date.none.fl_str_mv 2020-01-01
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132020000100407
url http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132020000100407
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv 10.1590/1678-4324-2020190359
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv text/html
dc.publisher.none.fl_str_mv Instituto de Tecnologia do Paraná - Tecpar
publisher.none.fl_str_mv Instituto de Tecnologia do Paraná - Tecpar
dc.source.none.fl_str_mv Brazilian Archives of Biology and Technology v.63 2020
reponame:Brazilian Archives of Biology and Technology
instname:Instituto de Tecnologia do Paraná (Tecpar)
instacron:TECPAR
instname_str Instituto de Tecnologia do Paraná (Tecpar)
instacron_str TECPAR
institution TECPAR
reponame_str Brazilian Archives of Biology and Technology
collection Brazilian Archives of Biology and Technology
repository.name.fl_str_mv Brazilian Archives of Biology and Technology - Instituto de Tecnologia do Paraná (Tecpar)
repository.mail.fl_str_mv babt@tecpar.br||babt@tecpar.br
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