Microscale direct transesterification of microbial biomass with ethanol for screening of microorganisms by its fatty acid content
Autor(a) principal: | |
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Data de Publicação: | 2019 |
Outros Autores: | , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Brazilian Archives of Biology and Technology |
Texto Completo: | http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132019000100401 |
Resumo: | Abstract We present an improved method of direct transesterification suitable for the quantitative analysis of multiple dry samples for its fatty acid content, using a minimal amount of biomass and reactants. The method features an acid-catalyzed direct alcoholysis of microgram samples of dry biomass; the rationale behind the solvent and reagent proportions chosen is discussed. The method was validated using seven microbial strains with diverse lipid content (Saccharomyces cerevisiae, Saccharomyces boulardii, Candida tropicalis, Haematococcus pluvialis, Chlorella vulgaris, Spirulina platensis and Schizochytrium limacinum), and compared with a macroscale direct transesterification method, and with gravimetric analysis of lipids extracted with solvents. The microscale method showed a conversion of 98.06 ± 0.87% of the lipids, using approximately 3 mg of dry biomass, 1mL of 0.2M H2SO4 dissolved in anhydrous ethanol (the acid is the catalyzer and ethanol the reactant)). The mixture was maintained at 70 °C for 20 h with periodic mixing, and then extracted with 2mL n-heptane and analyzed by GC-FID. The lipid content was then calculated considering dilution and sample mass. This method is effective, reliable, and technically attractive for analytical and comparative purposes. |
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Brazilian Archives of Biology and Technology |
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Microscale direct transesterification of microbial biomass with ethanol for screening of microorganisms by its fatty acid contentmicrobial oilethanolysisFAEEmicroscalescreeningAbstract We present an improved method of direct transesterification suitable for the quantitative analysis of multiple dry samples for its fatty acid content, using a minimal amount of biomass and reactants. The method features an acid-catalyzed direct alcoholysis of microgram samples of dry biomass; the rationale behind the solvent and reagent proportions chosen is discussed. The method was validated using seven microbial strains with diverse lipid content (Saccharomyces cerevisiae, Saccharomyces boulardii, Candida tropicalis, Haematococcus pluvialis, Chlorella vulgaris, Spirulina platensis and Schizochytrium limacinum), and compared with a macroscale direct transesterification method, and with gravimetric analysis of lipids extracted with solvents. The microscale method showed a conversion of 98.06 ± 0.87% of the lipids, using approximately 3 mg of dry biomass, 1mL of 0.2M H2SO4 dissolved in anhydrous ethanol (the acid is the catalyzer and ethanol the reactant)). The mixture was maintained at 70 °C for 20 h with periodic mixing, and then extracted with 2mL n-heptane and analyzed by GC-FID. The lipid content was then calculated considering dilution and sample mass. This method is effective, reliable, and technically attractive for analytical and comparative purposes.Instituto de Tecnologia do Paraná - Tecpar2019-01-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersiontext/htmlhttp://old.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132019000100401Brazilian Archives of Biology and Technology v.62 2019reponame:Brazilian Archives of Biology and Technologyinstname:Instituto de Tecnologia do Paraná (Tecpar)instacron:TECPAR10.1590/1678-4324-2019180178info:eu-repo/semantics/openAccessSorgatto,Vanessa GhiggiCarvalho,Julio Cesar deSydney,Eduardo BittencourtMedeiros,Adriane Bianchi PedroniVandenberghe,Luciana Porto de SouzaSoccol,Carlos Ricardoeng2019-04-11T00:00:00Zoai:scielo:S1516-89132019000100401Revistahttps://www.scielo.br/j/babt/https://old.scielo.br/oai/scielo-oai.phpbabt@tecpar.br||babt@tecpar.br1678-43241516-8913opendoar:2019-04-11T00:00Brazilian Archives of Biology and Technology - Instituto de Tecnologia do Paraná (Tecpar)false |
dc.title.none.fl_str_mv |
Microscale direct transesterification of microbial biomass with ethanol for screening of microorganisms by its fatty acid content |
title |
Microscale direct transesterification of microbial biomass with ethanol for screening of microorganisms by its fatty acid content |
spellingShingle |
Microscale direct transesterification of microbial biomass with ethanol for screening of microorganisms by its fatty acid content Sorgatto,Vanessa Ghiggi microbial oil ethanolysis FAEE microscale screening |
title_short |
Microscale direct transesterification of microbial biomass with ethanol for screening of microorganisms by its fatty acid content |
title_full |
Microscale direct transesterification of microbial biomass with ethanol for screening of microorganisms by its fatty acid content |
title_fullStr |
Microscale direct transesterification of microbial biomass with ethanol for screening of microorganisms by its fatty acid content |
title_full_unstemmed |
Microscale direct transesterification of microbial biomass with ethanol for screening of microorganisms by its fatty acid content |
title_sort |
Microscale direct transesterification of microbial biomass with ethanol for screening of microorganisms by its fatty acid content |
author |
Sorgatto,Vanessa Ghiggi |
author_facet |
Sorgatto,Vanessa Ghiggi Carvalho,Julio Cesar de Sydney,Eduardo Bittencourt Medeiros,Adriane Bianchi Pedroni Vandenberghe,Luciana Porto de Souza Soccol,Carlos Ricardo |
author_role |
author |
author2 |
Carvalho,Julio Cesar de Sydney,Eduardo Bittencourt Medeiros,Adriane Bianchi Pedroni Vandenberghe,Luciana Porto de Souza Soccol,Carlos Ricardo |
author2_role |
author author author author author |
dc.contributor.author.fl_str_mv |
Sorgatto,Vanessa Ghiggi Carvalho,Julio Cesar de Sydney,Eduardo Bittencourt Medeiros,Adriane Bianchi Pedroni Vandenberghe,Luciana Porto de Souza Soccol,Carlos Ricardo |
dc.subject.por.fl_str_mv |
microbial oil ethanolysis FAEE microscale screening |
topic |
microbial oil ethanolysis FAEE microscale screening |
description |
Abstract We present an improved method of direct transesterification suitable for the quantitative analysis of multiple dry samples for its fatty acid content, using a minimal amount of biomass and reactants. The method features an acid-catalyzed direct alcoholysis of microgram samples of dry biomass; the rationale behind the solvent and reagent proportions chosen is discussed. The method was validated using seven microbial strains with diverse lipid content (Saccharomyces cerevisiae, Saccharomyces boulardii, Candida tropicalis, Haematococcus pluvialis, Chlorella vulgaris, Spirulina platensis and Schizochytrium limacinum), and compared with a macroscale direct transesterification method, and with gravimetric analysis of lipids extracted with solvents. The microscale method showed a conversion of 98.06 ± 0.87% of the lipids, using approximately 3 mg of dry biomass, 1mL of 0.2M H2SO4 dissolved in anhydrous ethanol (the acid is the catalyzer and ethanol the reactant)). The mixture was maintained at 70 °C for 20 h with periodic mixing, and then extracted with 2mL n-heptane and analyzed by GC-FID. The lipid content was then calculated considering dilution and sample mass. This method is effective, reliable, and technically attractive for analytical and comparative purposes. |
publishDate |
2019 |
dc.date.none.fl_str_mv |
2019-01-01 |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132019000100401 |
url |
http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132019000100401 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
10.1590/1678-4324-2019180178 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
text/html |
dc.publisher.none.fl_str_mv |
Instituto de Tecnologia do Paraná - Tecpar |
publisher.none.fl_str_mv |
Instituto de Tecnologia do Paraná - Tecpar |
dc.source.none.fl_str_mv |
Brazilian Archives of Biology and Technology v.62 2019 reponame:Brazilian Archives of Biology and Technology instname:Instituto de Tecnologia do Paraná (Tecpar) instacron:TECPAR |
instname_str |
Instituto de Tecnologia do Paraná (Tecpar) |
instacron_str |
TECPAR |
institution |
TECPAR |
reponame_str |
Brazilian Archives of Biology and Technology |
collection |
Brazilian Archives of Biology and Technology |
repository.name.fl_str_mv |
Brazilian Archives of Biology and Technology - Instituto de Tecnologia do Paraná (Tecpar) |
repository.mail.fl_str_mv |
babt@tecpar.br||babt@tecpar.br |
_version_ |
1750318279199555584 |