In vitro establishment and multiplication of the Normania triphylla (Lowe) Lowe
Autor(a) principal: | |
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Data de Publicação: | 2012 |
Outros Autores: | , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Brazilian Archives of Biology and Technology |
Texto Completo: | http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132012000400009 |
Resumo: | Normania triphylla is an endemic species from Madeira island (Portugal) extinct in the wild since 1991. The aim of this work was to culture the meristems of this species in vitro and to multiply its shoots in order to preserve this endangered species. The best results in terms of multiplication were obtained in Murashige and Skoog medium supplemented with 10 µM 6‑benzylaminopurine (BAP). The number of shoots, the number of nodes and the number of leaves were the most important in this medium. However, the best results concerning the total shoot length were obtained when BAP was not supplemented into the medium and in the presence of 5 or 7.5 µM 1-naphtalene acetic acid (NAA). This is the first report on the in vitro culture of N. triphylla which could brings new avenues for the development of this species. |
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In vitro establishment and multiplication of the Normania triphylla (Lowe) LoweNormaniaEndemic speciesMicropropagation6‑benzylaminopurineCallusNormania triphylla is an endemic species from Madeira island (Portugal) extinct in the wild since 1991. The aim of this work was to culture the meristems of this species in vitro and to multiply its shoots in order to preserve this endangered species. The best results in terms of multiplication were obtained in Murashige and Skoog medium supplemented with 10 µM 6‑benzylaminopurine (BAP). The number of shoots, the number of nodes and the number of leaves were the most important in this medium. However, the best results concerning the total shoot length were obtained when BAP was not supplemented into the medium and in the presence of 5 or 7.5 µM 1-naphtalene acetic acid (NAA). This is the first report on the in vitro culture of N. triphylla which could brings new avenues for the development of this species.Instituto de Tecnologia do Paraná - Tecpar2012-08-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersiontext/htmlhttp://old.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132012000400009Brazilian Archives of Biology and Technology v.55 n.4 2012reponame:Brazilian Archives of Biology and Technologyinstname:Instituto de Tecnologia do Paraná (Tecpar)instacron:TECPAR10.1590/S1516-89132012000400009info:eu-repo/semantics/openAccessDelmail,DavidLabrousse,PascalLesouëf,Jean-YvesLe Hir,FrançoisAutret,Jean-LucBotineau,Micheleng2012-08-16T00:00:00Zoai:scielo:S1516-89132012000400009Revistahttps://www.scielo.br/j/babt/https://old.scielo.br/oai/scielo-oai.phpbabt@tecpar.br||babt@tecpar.br1678-43241516-8913opendoar:2012-08-16T00:00Brazilian Archives of Biology and Technology - Instituto de Tecnologia do Paraná (Tecpar)false |
dc.title.none.fl_str_mv |
In vitro establishment and multiplication of the Normania triphylla (Lowe) Lowe |
title |
In vitro establishment and multiplication of the Normania triphylla (Lowe) Lowe |
spellingShingle |
In vitro establishment and multiplication of the Normania triphylla (Lowe) Lowe Delmail,David Normania Endemic species Micropropagation 6‑benzylaminopurine Callus |
title_short |
In vitro establishment and multiplication of the Normania triphylla (Lowe) Lowe |
title_full |
In vitro establishment and multiplication of the Normania triphylla (Lowe) Lowe |
title_fullStr |
In vitro establishment and multiplication of the Normania triphylla (Lowe) Lowe |
title_full_unstemmed |
In vitro establishment and multiplication of the Normania triphylla (Lowe) Lowe |
title_sort |
In vitro establishment and multiplication of the Normania triphylla (Lowe) Lowe |
author |
Delmail,David |
author_facet |
Delmail,David Labrousse,Pascal Lesouëf,Jean-Yves Le Hir,François Autret,Jean-Luc Botineau,Michel |
author_role |
author |
author2 |
Labrousse,Pascal Lesouëf,Jean-Yves Le Hir,François Autret,Jean-Luc Botineau,Michel |
author2_role |
author author author author author |
dc.contributor.author.fl_str_mv |
Delmail,David Labrousse,Pascal Lesouëf,Jean-Yves Le Hir,François Autret,Jean-Luc Botineau,Michel |
dc.subject.por.fl_str_mv |
Normania Endemic species Micropropagation 6‑benzylaminopurine Callus |
topic |
Normania Endemic species Micropropagation 6‑benzylaminopurine Callus |
description |
Normania triphylla is an endemic species from Madeira island (Portugal) extinct in the wild since 1991. The aim of this work was to culture the meristems of this species in vitro and to multiply its shoots in order to preserve this endangered species. The best results in terms of multiplication were obtained in Murashige and Skoog medium supplemented with 10 µM 6‑benzylaminopurine (BAP). The number of shoots, the number of nodes and the number of leaves were the most important in this medium. However, the best results concerning the total shoot length were obtained when BAP was not supplemented into the medium and in the presence of 5 or 7.5 µM 1-naphtalene acetic acid (NAA). This is the first report on the in vitro culture of N. triphylla which could brings new avenues for the development of this species. |
publishDate |
2012 |
dc.date.none.fl_str_mv |
2012-08-01 |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132012000400009 |
url |
http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132012000400009 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
10.1590/S1516-89132012000400009 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
text/html |
dc.publisher.none.fl_str_mv |
Instituto de Tecnologia do Paraná - Tecpar |
publisher.none.fl_str_mv |
Instituto de Tecnologia do Paraná - Tecpar |
dc.source.none.fl_str_mv |
Brazilian Archives of Biology and Technology v.55 n.4 2012 reponame:Brazilian Archives of Biology and Technology instname:Instituto de Tecnologia do Paraná (Tecpar) instacron:TECPAR |
instname_str |
Instituto de Tecnologia do Paraná (Tecpar) |
instacron_str |
TECPAR |
institution |
TECPAR |
reponame_str |
Brazilian Archives of Biology and Technology |
collection |
Brazilian Archives of Biology and Technology |
repository.name.fl_str_mv |
Brazilian Archives of Biology and Technology - Instituto de Tecnologia do Paraná (Tecpar) |
repository.mail.fl_str_mv |
babt@tecpar.br||babt@tecpar.br |
_version_ |
1750318275240132608 |