Scale up of production in a bioreactor of a halotolerant protease from moderately halophilic Bacillus sp. isolated from soil
Autor(a) principal: | |
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Data de Publicação: | 2014 |
Outros Autores: | , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Brazilian Archives of Biology and Technology |
Texto Completo: | http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132014000300020 |
Resumo: | Studies were conducted on the production of protease by moderately halophilic Bacillus sp. on agro-industrial waste materials. The bacterium could efficiently use many agro wastes as substrates but wheat bran supported maximum enzyme production. To ascertain the performance of the process in shake flasks and lab scale bioreactor, experiments were conducted to analyse protease activity utilizing wheat bran as cost effective substrate. The studies unveiled that pH 7.0, temperature 30°C and static conditions were optimal for enzyme production in flask level fermentation. In scale-up fermentation, at optimal pH and temperature, agitation rate of 50 rpm was best for protease production. The enzymatic nature was studied in 10% SDS gels with BSA (2.5 mg/mL) as substrate and banding pattern was compared with undigested BSA as control. The endoprotease nature and the kinetics of protease activity were confirmed. The enzyme retained 37% of its activity even at 5 M NaCl concentration. The proteolytic activity was also confirmed by casein zymogram analysis. The fermentation medium containing inexpensive substrates, physical conditions and ability of Bacillus sp. to exhibit protease activity on a large scale could collectively be useful for commercial production. |
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Scale up of production in a bioreactor of a halotolerant protease from moderately halophilic Bacillus sp. isolated from soilPoultry soilendoproteasescale up fermentationwheat branhalotolerantStudies were conducted on the production of protease by moderately halophilic Bacillus sp. on agro-industrial waste materials. The bacterium could efficiently use many agro wastes as substrates but wheat bran supported maximum enzyme production. To ascertain the performance of the process in shake flasks and lab scale bioreactor, experiments were conducted to analyse protease activity utilizing wheat bran as cost effective substrate. The studies unveiled that pH 7.0, temperature 30°C and static conditions were optimal for enzyme production in flask level fermentation. In scale-up fermentation, at optimal pH and temperature, agitation rate of 50 rpm was best for protease production. The enzymatic nature was studied in 10% SDS gels with BSA (2.5 mg/mL) as substrate and banding pattern was compared with undigested BSA as control. The endoprotease nature and the kinetics of protease activity were confirmed. The enzyme retained 37% of its activity even at 5 M NaCl concentration. The proteolytic activity was also confirmed by casein zymogram analysis. The fermentation medium containing inexpensive substrates, physical conditions and ability of Bacillus sp. to exhibit protease activity on a large scale could collectively be useful for commercial production.Instituto de Tecnologia do Paraná - Tecpar2014-06-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersiontext/htmlhttp://old.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132014000300020Brazilian Archives of Biology and Technology v.57 n.3 2014reponame:Brazilian Archives of Biology and Technologyinstname:Instituto de Tecnologia do Paraná (Tecpar)instacron:TECPAR10.1590/S1516-89132014005000014info:eu-repo/semantics/openAccessPrasad,RoopaAbraham,Theruvath KoshyNair,Ananthakrishnan Jayakumaraneng2014-05-30T00:00:00Zoai:scielo:S1516-89132014000300020Revistahttps://www.scielo.br/j/babt/https://old.scielo.br/oai/scielo-oai.phpbabt@tecpar.br||babt@tecpar.br1678-43241516-8913opendoar:2014-05-30T00:00Brazilian Archives of Biology and Technology - Instituto de Tecnologia do Paraná (Tecpar)false |
dc.title.none.fl_str_mv |
Scale up of production in a bioreactor of a halotolerant protease from moderately halophilic Bacillus sp. isolated from soil |
title |
Scale up of production in a bioreactor of a halotolerant protease from moderately halophilic Bacillus sp. isolated from soil |
spellingShingle |
Scale up of production in a bioreactor of a halotolerant protease from moderately halophilic Bacillus sp. isolated from soil Prasad,Roopa Poultry soil endoprotease scale up fermentation wheat bran halotolerant |
title_short |
Scale up of production in a bioreactor of a halotolerant protease from moderately halophilic Bacillus sp. isolated from soil |
title_full |
Scale up of production in a bioreactor of a halotolerant protease from moderately halophilic Bacillus sp. isolated from soil |
title_fullStr |
Scale up of production in a bioreactor of a halotolerant protease from moderately halophilic Bacillus sp. isolated from soil |
title_full_unstemmed |
Scale up of production in a bioreactor of a halotolerant protease from moderately halophilic Bacillus sp. isolated from soil |
title_sort |
Scale up of production in a bioreactor of a halotolerant protease from moderately halophilic Bacillus sp. isolated from soil |
author |
Prasad,Roopa |
author_facet |
Prasad,Roopa Abraham,Theruvath Koshy Nair,Ananthakrishnan Jayakumaran |
author_role |
author |
author2 |
Abraham,Theruvath Koshy Nair,Ananthakrishnan Jayakumaran |
author2_role |
author author |
dc.contributor.author.fl_str_mv |
Prasad,Roopa Abraham,Theruvath Koshy Nair,Ananthakrishnan Jayakumaran |
dc.subject.por.fl_str_mv |
Poultry soil endoprotease scale up fermentation wheat bran halotolerant |
topic |
Poultry soil endoprotease scale up fermentation wheat bran halotolerant |
description |
Studies were conducted on the production of protease by moderately halophilic Bacillus sp. on agro-industrial waste materials. The bacterium could efficiently use many agro wastes as substrates but wheat bran supported maximum enzyme production. To ascertain the performance of the process in shake flasks and lab scale bioreactor, experiments were conducted to analyse protease activity utilizing wheat bran as cost effective substrate. The studies unveiled that pH 7.0, temperature 30°C and static conditions were optimal for enzyme production in flask level fermentation. In scale-up fermentation, at optimal pH and temperature, agitation rate of 50 rpm was best for protease production. The enzymatic nature was studied in 10% SDS gels with BSA (2.5 mg/mL) as substrate and banding pattern was compared with undigested BSA as control. The endoprotease nature and the kinetics of protease activity were confirmed. The enzyme retained 37% of its activity even at 5 M NaCl concentration. The proteolytic activity was also confirmed by casein zymogram analysis. The fermentation medium containing inexpensive substrates, physical conditions and ability of Bacillus sp. to exhibit protease activity on a large scale could collectively be useful for commercial production. |
publishDate |
2014 |
dc.date.none.fl_str_mv |
2014-06-01 |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132014000300020 |
url |
http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132014000300020 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
10.1590/S1516-89132014005000014 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
text/html |
dc.publisher.none.fl_str_mv |
Instituto de Tecnologia do Paraná - Tecpar |
publisher.none.fl_str_mv |
Instituto de Tecnologia do Paraná - Tecpar |
dc.source.none.fl_str_mv |
Brazilian Archives of Biology and Technology v.57 n.3 2014 reponame:Brazilian Archives of Biology and Technology instname:Instituto de Tecnologia do Paraná (Tecpar) instacron:TECPAR |
instname_str |
Instituto de Tecnologia do Paraná (Tecpar) |
instacron_str |
TECPAR |
institution |
TECPAR |
reponame_str |
Brazilian Archives of Biology and Technology |
collection |
Brazilian Archives of Biology and Technology |
repository.name.fl_str_mv |
Brazilian Archives of Biology and Technology - Instituto de Tecnologia do Paraná (Tecpar) |
repository.mail.fl_str_mv |
babt@tecpar.br||babt@tecpar.br |
_version_ |
1750318276537221120 |