Genetic diversity among forty coffee varieties assessed by RAPD markers associated with restriction digestion
Autor(a) principal: | |
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Data de Publicação: | 2005 |
Outros Autores: | , , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Brazilian Archives of Biology and Technology |
Texto Completo: | http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132005000500002 |
Resumo: | The genetic variability of 40 accessions of_C. arabica was evaluated using a combination of the random amplified polymorphic DNA (RAPD) technique and restriction digestion of genomic DNA. The genetic variability and the relatedness among all accessions were initially evaluated using 195 RAPD primers which revealed a very low level of genetic variation. To improve the efficiency in the detection of polymorphism, the genomic DNA of all accessions were submitted to digestion with restriction endonucleases prior to PCR amplification. A total of 24 primers combined with restriction digestion of DNA rendered 318 bands, of which 266 (83.65%) were polymorphic. The associations among genotypes were estimated using UPGMA-clustering analysis. The accessions were properly clustered according to pedigree and agronomic features. The ability to distinguish among coffee accessions was greater for RAPD plus restriction digestion than for RAPD alone, providing evidences that the combination of the techniques was very efficient for the estimation of genetic relationship among_C. arabica genotypes. |
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Brazilian Archives of Biology and Technology |
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Genetic diversity among forty coffee varieties assessed by RAPD markers associated with restriction digestionCoffea arabicagenetic variabilityRAPDrestriction endonucleasesThe genetic variability of 40 accessions of_C. arabica was evaluated using a combination of the random amplified polymorphic DNA (RAPD) technique and restriction digestion of genomic DNA. The genetic variability and the relatedness among all accessions were initially evaluated using 195 RAPD primers which revealed a very low level of genetic variation. To improve the efficiency in the detection of polymorphism, the genomic DNA of all accessions were submitted to digestion with restriction endonucleases prior to PCR amplification. A total of 24 primers combined with restriction digestion of DNA rendered 318 bands, of which 266 (83.65%) were polymorphic. The associations among genotypes were estimated using UPGMA-clustering analysis. The accessions were properly clustered according to pedigree and agronomic features. The ability to distinguish among coffee accessions was greater for RAPD plus restriction digestion than for RAPD alone, providing evidences that the combination of the techniques was very efficient for the estimation of genetic relationship among_C. arabica genotypes.Instituto de Tecnologia do Paraná - Tecpar2005-07-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersiontext/htmlhttp://old.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132005000500002Brazilian Archives of Biology and Technology v.48 n.4 2005reponame:Brazilian Archives of Biology and Technologyinstname:Instituto de Tecnologia do Paraná (Tecpar)instacron:TECPAR10.1590/S1516-89132005000500002info:eu-repo/semantics/openAccessDiniz,Leandro Eugênio CardamoniRuas,Claudete de FátimaCarvalho,Valdemar de PaulaTorres,Fabrício MedeirosRuas,Eduardo AugustoSantos,Melissa de OliveiraSera,TumoruRuas,Paulo Maurícioeng2005-09-14T00:00:00Zoai:scielo:S1516-89132005000500002Revistahttps://www.scielo.br/j/babt/https://old.scielo.br/oai/scielo-oai.phpbabt@tecpar.br||babt@tecpar.br1678-43241516-8913opendoar:2005-09-14T00:00Brazilian Archives of Biology and Technology - Instituto de Tecnologia do Paraná (Tecpar)false |
dc.title.none.fl_str_mv |
Genetic diversity among forty coffee varieties assessed by RAPD markers associated with restriction digestion |
title |
Genetic diversity among forty coffee varieties assessed by RAPD markers associated with restriction digestion |
spellingShingle |
Genetic diversity among forty coffee varieties assessed by RAPD markers associated with restriction digestion Diniz,Leandro Eugênio Cardamoni Coffea arabica genetic variability RAPD restriction endonucleases |
title_short |
Genetic diversity among forty coffee varieties assessed by RAPD markers associated with restriction digestion |
title_full |
Genetic diversity among forty coffee varieties assessed by RAPD markers associated with restriction digestion |
title_fullStr |
Genetic diversity among forty coffee varieties assessed by RAPD markers associated with restriction digestion |
title_full_unstemmed |
Genetic diversity among forty coffee varieties assessed by RAPD markers associated with restriction digestion |
title_sort |
Genetic diversity among forty coffee varieties assessed by RAPD markers associated with restriction digestion |
author |
Diniz,Leandro Eugênio Cardamoni |
author_facet |
Diniz,Leandro Eugênio Cardamoni Ruas,Claudete de Fátima Carvalho,Valdemar de Paula Torres,Fabrício Medeiros Ruas,Eduardo Augusto Santos,Melissa de Oliveira Sera,Tumoru Ruas,Paulo Maurício |
author_role |
author |
author2 |
Ruas,Claudete de Fátima Carvalho,Valdemar de Paula Torres,Fabrício Medeiros Ruas,Eduardo Augusto Santos,Melissa de Oliveira Sera,Tumoru Ruas,Paulo Maurício |
author2_role |
author author author author author author author |
dc.contributor.author.fl_str_mv |
Diniz,Leandro Eugênio Cardamoni Ruas,Claudete de Fátima Carvalho,Valdemar de Paula Torres,Fabrício Medeiros Ruas,Eduardo Augusto Santos,Melissa de Oliveira Sera,Tumoru Ruas,Paulo Maurício |
dc.subject.por.fl_str_mv |
Coffea arabica genetic variability RAPD restriction endonucleases |
topic |
Coffea arabica genetic variability RAPD restriction endonucleases |
description |
The genetic variability of 40 accessions of_C. arabica was evaluated using a combination of the random amplified polymorphic DNA (RAPD) technique and restriction digestion of genomic DNA. The genetic variability and the relatedness among all accessions were initially evaluated using 195 RAPD primers which revealed a very low level of genetic variation. To improve the efficiency in the detection of polymorphism, the genomic DNA of all accessions were submitted to digestion with restriction endonucleases prior to PCR amplification. A total of 24 primers combined with restriction digestion of DNA rendered 318 bands, of which 266 (83.65%) were polymorphic. The associations among genotypes were estimated using UPGMA-clustering analysis. The accessions were properly clustered according to pedigree and agronomic features. The ability to distinguish among coffee accessions was greater for RAPD plus restriction digestion than for RAPD alone, providing evidences that the combination of the techniques was very efficient for the estimation of genetic relationship among_C. arabica genotypes. |
publishDate |
2005 |
dc.date.none.fl_str_mv |
2005-07-01 |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132005000500002 |
url |
http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132005000500002 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
10.1590/S1516-89132005000500002 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
text/html |
dc.publisher.none.fl_str_mv |
Instituto de Tecnologia do Paraná - Tecpar |
publisher.none.fl_str_mv |
Instituto de Tecnologia do Paraná - Tecpar |
dc.source.none.fl_str_mv |
Brazilian Archives of Biology and Technology v.48 n.4 2005 reponame:Brazilian Archives of Biology and Technology instname:Instituto de Tecnologia do Paraná (Tecpar) instacron:TECPAR |
instname_str |
Instituto de Tecnologia do Paraná (Tecpar) |
instacron_str |
TECPAR |
institution |
TECPAR |
reponame_str |
Brazilian Archives of Biology and Technology |
collection |
Brazilian Archives of Biology and Technology |
repository.name.fl_str_mv |
Brazilian Archives of Biology and Technology - Instituto de Tecnologia do Paraná (Tecpar) |
repository.mail.fl_str_mv |
babt@tecpar.br||babt@tecpar.br |
_version_ |
1750318270338039808 |