Real time PCR and importance of housekeepings genes for normalization and quantification of mRNA expression in different tissues
Autor(a) principal: | |
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Data de Publicação: | 2013 |
Outros Autores: | , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Brazilian Archives of Biology and Technology |
Texto Completo: | http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132013000100019 |
Resumo: | The aim of this review was to evaluate the importance of the real-time PCR (qRT-PCR) as a technique for mRNA expression analysis in different tissues. Real-time PCR is widely used for quantification of mRNA levels and is a fundamental tool for basic research, molecular medicine and biotechnology.Genes of references are expressed in a wide variety of tissues and cells with minimal variations in their expression levels, and thus are used to normalize data of mRNA quantification. Software programs, such as geNorm, BestKeeper and NormFinder, have been developed to perform the normalization of data, which help to choose the most stable reference gene. Several genes, such as GAPDH, β-actin, β-tubulin, PGK, UBQ, RPL-19 and 18S rRNA have been suggested as standards in PCR studies, but these genes can have variation in their expression in different tissues, reinforcing the idea that there is no ideal reference gene. |
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Brazilian Archives of Biology and Technology |
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Real time PCR and importance of housekeepings genes for normalization and quantification of mRNA expression in different tissuesPCRnormalizationreference genesgene expressionmRNAThe aim of this review was to evaluate the importance of the real-time PCR (qRT-PCR) as a technique for mRNA expression analysis in different tissues. Real-time PCR is widely used for quantification of mRNA levels and is a fundamental tool for basic research, molecular medicine and biotechnology.Genes of references are expressed in a wide variety of tissues and cells with minimal variations in their expression levels, and thus are used to normalize data of mRNA quantification. Software programs, such as geNorm, BestKeeper and NormFinder, have been developed to perform the normalization of data, which help to choose the most stable reference gene. Several genes, such as GAPDH, β-actin, β-tubulin, PGK, UBQ, RPL-19 and 18S rRNA have been suggested as standards in PCR studies, but these genes can have variation in their expression in different tissues, reinforcing the idea that there is no ideal reference gene.Instituto de Tecnologia do Paraná - Tecpar2013-02-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersiontext/htmlhttp://old.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132013000100019Brazilian Archives of Biology and Technology v.56 n.1 2013reponame:Brazilian Archives of Biology and Technologyinstname:Instituto de Tecnologia do Paraná (Tecpar)instacron:TECPAR10.1590/S1516-89132013000100019info:eu-repo/semantics/openAccessRebouças,Emanuela de LimaCosta,José Jackson do NascimentoPassos,Maria JulianePassos,José Renato de SousaHurk,Robert van denSilva,José Roberto Vianaeng2013-04-09T00:00:00Zoai:scielo:S1516-89132013000100019Revistahttps://www.scielo.br/j/babt/https://old.scielo.br/oai/scielo-oai.phpbabt@tecpar.br||babt@tecpar.br1678-43241516-8913opendoar:2013-04-09T00:00Brazilian Archives of Biology and Technology - Instituto de Tecnologia do Paraná (Tecpar)false |
dc.title.none.fl_str_mv |
Real time PCR and importance of housekeepings genes for normalization and quantification of mRNA expression in different tissues |
title |
Real time PCR and importance of housekeepings genes for normalization and quantification of mRNA expression in different tissues |
spellingShingle |
Real time PCR and importance of housekeepings genes for normalization and quantification of mRNA expression in different tissues Rebouças,Emanuela de Lima PCR normalization reference genes gene expression mRNA |
title_short |
Real time PCR and importance of housekeepings genes for normalization and quantification of mRNA expression in different tissues |
title_full |
Real time PCR and importance of housekeepings genes for normalization and quantification of mRNA expression in different tissues |
title_fullStr |
Real time PCR and importance of housekeepings genes for normalization and quantification of mRNA expression in different tissues |
title_full_unstemmed |
Real time PCR and importance of housekeepings genes for normalization and quantification of mRNA expression in different tissues |
title_sort |
Real time PCR and importance of housekeepings genes for normalization and quantification of mRNA expression in different tissues |
author |
Rebouças,Emanuela de Lima |
author_facet |
Rebouças,Emanuela de Lima Costa,José Jackson do Nascimento Passos,Maria Juliane Passos,José Renato de Sousa Hurk,Robert van den Silva,José Roberto Viana |
author_role |
author |
author2 |
Costa,José Jackson do Nascimento Passos,Maria Juliane Passos,José Renato de Sousa Hurk,Robert van den Silva,José Roberto Viana |
author2_role |
author author author author author |
dc.contributor.author.fl_str_mv |
Rebouças,Emanuela de Lima Costa,José Jackson do Nascimento Passos,Maria Juliane Passos,José Renato de Sousa Hurk,Robert van den Silva,José Roberto Viana |
dc.subject.por.fl_str_mv |
PCR normalization reference genes gene expression mRNA |
topic |
PCR normalization reference genes gene expression mRNA |
description |
The aim of this review was to evaluate the importance of the real-time PCR (qRT-PCR) as a technique for mRNA expression analysis in different tissues. Real-time PCR is widely used for quantification of mRNA levels and is a fundamental tool for basic research, molecular medicine and biotechnology.Genes of references are expressed in a wide variety of tissues and cells with minimal variations in their expression levels, and thus are used to normalize data of mRNA quantification. Software programs, such as geNorm, BestKeeper and NormFinder, have been developed to perform the normalization of data, which help to choose the most stable reference gene. Several genes, such as GAPDH, β-actin, β-tubulin, PGK, UBQ, RPL-19 and 18S rRNA have been suggested as standards in PCR studies, but these genes can have variation in their expression in different tissues, reinforcing the idea that there is no ideal reference gene. |
publishDate |
2013 |
dc.date.none.fl_str_mv |
2013-02-01 |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132013000100019 |
url |
http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132013000100019 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
10.1590/S1516-89132013000100019 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
text/html |
dc.publisher.none.fl_str_mv |
Instituto de Tecnologia do Paraná - Tecpar |
publisher.none.fl_str_mv |
Instituto de Tecnologia do Paraná - Tecpar |
dc.source.none.fl_str_mv |
Brazilian Archives of Biology and Technology v.56 n.1 2013 reponame:Brazilian Archives of Biology and Technology instname:Instituto de Tecnologia do Paraná (Tecpar) instacron:TECPAR |
instname_str |
Instituto de Tecnologia do Paraná (Tecpar) |
instacron_str |
TECPAR |
institution |
TECPAR |
reponame_str |
Brazilian Archives of Biology and Technology |
collection |
Brazilian Archives of Biology and Technology |
repository.name.fl_str_mv |
Brazilian Archives of Biology and Technology - Instituto de Tecnologia do Paraná (Tecpar) |
repository.mail.fl_str_mv |
babt@tecpar.br||babt@tecpar.br |
_version_ |
1750318275602939904 |