Thermostabilisation of human serum butyrylcholinesterase for detection of its inhibitors in water and biological fluids

Detalhes bibliográficos
Autor(a) principal: Jaganathan,Lakshmanan
Data de Publicação: 1999
Outros Autores: Boopathy,Rathanam
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Brazilian Archives of Biology and Technology
Texto Completo: http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89131999000300012
Resumo: The ability of gelatine-trehalose to convert the normally fragile, dry human serum BChE into a thermostable enzyme and its use in the detection of cholinesterase inhibitors in water and biological fluids is described. Gelatine or trehalose alone is unable to protect the dry enzyme against exposure to high temperature, while a combination of gelatine and trehalose were able to protect the enzyme activity against prolonged exposure to temperature as high as +50°C. A method for rapid, simple and inexpensive means of screening for cholinesterase inhibitors such as carbamates and organophosphates in water, vegetables and human blood has been developed.
id TECPAR-1_deef9b986e885dfcf7bd11138181bd2b
oai_identifier_str oai:scielo:S1516-89131999000300012
network_acronym_str TECPAR-1
network_name_str Brazilian Archives of Biology and Technology
repository_id_str
spelling Thermostabilisation of human serum butyrylcholinesterase for detection of its inhibitors in water and biological fluidsButyrylcholinesterasecarbamatescholinesterasegelatineorganophosphorustrehaloseThe ability of gelatine-trehalose to convert the normally fragile, dry human serum BChE into a thermostable enzyme and its use in the detection of cholinesterase inhibitors in water and biological fluids is described. Gelatine or trehalose alone is unable to protect the dry enzyme against exposure to high temperature, while a combination of gelatine and trehalose were able to protect the enzyme activity against prolonged exposure to temperature as high as +50°C. A method for rapid, simple and inexpensive means of screening for cholinesterase inhibitors such as carbamates and organophosphates in water, vegetables and human blood has been developed.Instituto de Tecnologia do Paraná - Tecpar1999-01-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersiontext/htmlhttp://old.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89131999000300012Brazilian Archives of Biology and Technology v.42 n.3 1999reponame:Brazilian Archives of Biology and Technologyinstname:Instituto de Tecnologia do Paraná (Tecpar)instacron:TECPAR10.1590/S1516-89131999000300012info:eu-repo/semantics/openAccessJaganathan,LakshmananBoopathy,Rathanameng2011-06-01T00:00:00Zoai:scielo:S1516-89131999000300012Revistahttps://www.scielo.br/j/babt/https://old.scielo.br/oai/scielo-oai.phpbabt@tecpar.br||babt@tecpar.br1678-43241516-8913opendoar:2011-06-01T00:00Brazilian Archives of Biology and Technology - Instituto de Tecnologia do Paraná (Tecpar)false
dc.title.none.fl_str_mv Thermostabilisation of human serum butyrylcholinesterase for detection of its inhibitors in water and biological fluids
title Thermostabilisation of human serum butyrylcholinesterase for detection of its inhibitors in water and biological fluids
spellingShingle Thermostabilisation of human serum butyrylcholinesterase for detection of its inhibitors in water and biological fluids
Jaganathan,Lakshmanan
Butyrylcholinesterase
carbamates
cholinesterase
gelatine
organophosphorus
trehalose
title_short Thermostabilisation of human serum butyrylcholinesterase for detection of its inhibitors in water and biological fluids
title_full Thermostabilisation of human serum butyrylcholinesterase for detection of its inhibitors in water and biological fluids
title_fullStr Thermostabilisation of human serum butyrylcholinesterase for detection of its inhibitors in water and biological fluids
title_full_unstemmed Thermostabilisation of human serum butyrylcholinesterase for detection of its inhibitors in water and biological fluids
title_sort Thermostabilisation of human serum butyrylcholinesterase for detection of its inhibitors in water and biological fluids
author Jaganathan,Lakshmanan
author_facet Jaganathan,Lakshmanan
Boopathy,Rathanam
author_role author
author2 Boopathy,Rathanam
author2_role author
dc.contributor.author.fl_str_mv Jaganathan,Lakshmanan
Boopathy,Rathanam
dc.subject.por.fl_str_mv Butyrylcholinesterase
carbamates
cholinesterase
gelatine
organophosphorus
trehalose
topic Butyrylcholinesterase
carbamates
cholinesterase
gelatine
organophosphorus
trehalose
description The ability of gelatine-trehalose to convert the normally fragile, dry human serum BChE into a thermostable enzyme and its use in the detection of cholinesterase inhibitors in water and biological fluids is described. Gelatine or trehalose alone is unable to protect the dry enzyme against exposure to high temperature, while a combination of gelatine and trehalose were able to protect the enzyme activity against prolonged exposure to temperature as high as +50°C. A method for rapid, simple and inexpensive means of screening for cholinesterase inhibitors such as carbamates and organophosphates in water, vegetables and human blood has been developed.
publishDate 1999
dc.date.none.fl_str_mv 1999-01-01
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89131999000300012
url http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89131999000300012
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv 10.1590/S1516-89131999000300012
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv text/html
dc.publisher.none.fl_str_mv Instituto de Tecnologia do Paraná - Tecpar
publisher.none.fl_str_mv Instituto de Tecnologia do Paraná - Tecpar
dc.source.none.fl_str_mv Brazilian Archives of Biology and Technology v.42 n.3 1999
reponame:Brazilian Archives of Biology and Technology
instname:Instituto de Tecnologia do Paraná (Tecpar)
instacron:TECPAR
instname_str Instituto de Tecnologia do Paraná (Tecpar)
instacron_str TECPAR
institution TECPAR
reponame_str Brazilian Archives of Biology and Technology
collection Brazilian Archives of Biology and Technology
repository.name.fl_str_mv Brazilian Archives of Biology and Technology - Instituto de Tecnologia do Paraná (Tecpar)
repository.mail.fl_str_mv babt@tecpar.br||babt@tecpar.br
_version_ 1750318268215721984

Registros relacionados