Bivalent RNA interference to increase isoflavone biosynthesis in soybean (Glycine max)

Detalhes bibliográficos
Autor(a) principal: Jiang,Yina
Data de Publicação: 2014
Outros Autores: Hu,Yanlin, Wang,Biao, Wu,Tianlong
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Brazilian Archives of Biology and Technology
Texto Completo: http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132014000200002
Resumo: In this work, a bivalent RNA interference (RNAi) plant-transformation vector was constructed to silence both the flavanone 3-hydroxylase (F3H) gene and the flavone synthase II (GmFNSII) gene in soybean (Glycine max). Two further unit RNAi vectors were constructed for each of these two genes. RNAi-mediated suppression of these genes effectively regulated flavone and isoflavone production in hairy roots that arose from soybean cotyledons transformed with Agrobacterium rhizogenes ATCC15834. Notably, the bivalent RNAi vector had a significantly higher effect for increasing isoflavone production compared with the two unit RNAi vectors. The study highlighted molecular methods that could be used to enhance isoflavone production in soybean and demonstrated the challenges associated with such metabolic engineering for the production of plant natural products.
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spelling Bivalent RNA interference to increase isoflavone biosynthesis in soybean (Glycine max)Bivalent RNA interferenceFlavanone 3-hydroxylaseFlavone synthase IIIsoflavone biosynthesisSoybeanIn this work, a bivalent RNA interference (RNAi) plant-transformation vector was constructed to silence both the flavanone 3-hydroxylase (F3H) gene and the flavone synthase II (GmFNSII) gene in soybean (Glycine max). Two further unit RNAi vectors were constructed for each of these two genes. RNAi-mediated suppression of these genes effectively regulated flavone and isoflavone production in hairy roots that arose from soybean cotyledons transformed with Agrobacterium rhizogenes ATCC15834. Notably, the bivalent RNAi vector had a significantly higher effect for increasing isoflavone production compared with the two unit RNAi vectors. The study highlighted molecular methods that could be used to enhance isoflavone production in soybean and demonstrated the challenges associated with such metabolic engineering for the production of plant natural products.Instituto de Tecnologia do Paraná - Tecpar2014-04-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersiontext/htmlhttp://old.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132014000200002Brazilian Archives of Biology and Technology v.57 n.2 2014reponame:Brazilian Archives of Biology and Technologyinstname:Instituto de Tecnologia do Paraná (Tecpar)instacron:TECPAR10.1590/S1516-89132013005000018info:eu-repo/semantics/openAccessJiang,YinaHu,YanlinWang,BiaoWu,Tianlongeng2014-04-04T00:00:00Zoai:scielo:S1516-89132014000200002Revistahttps://www.scielo.br/j/babt/https://old.scielo.br/oai/scielo-oai.phpbabt@tecpar.br||babt@tecpar.br1678-43241516-8913opendoar:2014-04-04T00:00Brazilian Archives of Biology and Technology - Instituto de Tecnologia do Paraná (Tecpar)false
dc.title.none.fl_str_mv Bivalent RNA interference to increase isoflavone biosynthesis in soybean (Glycine max)
title Bivalent RNA interference to increase isoflavone biosynthesis in soybean (Glycine max)
spellingShingle Bivalent RNA interference to increase isoflavone biosynthesis in soybean (Glycine max)
Jiang,Yina
Bivalent RNA interference
Flavanone 3-hydroxylase
Flavone synthase II
Isoflavone biosynthesis
Soybean
title_short Bivalent RNA interference to increase isoflavone biosynthesis in soybean (Glycine max)
title_full Bivalent RNA interference to increase isoflavone biosynthesis in soybean (Glycine max)
title_fullStr Bivalent RNA interference to increase isoflavone biosynthesis in soybean (Glycine max)
title_full_unstemmed Bivalent RNA interference to increase isoflavone biosynthesis in soybean (Glycine max)
title_sort Bivalent RNA interference to increase isoflavone biosynthesis in soybean (Glycine max)
author Jiang,Yina
author_facet Jiang,Yina
Hu,Yanlin
Wang,Biao
Wu,Tianlong
author_role author
author2 Hu,Yanlin
Wang,Biao
Wu,Tianlong
author2_role author
author
author
dc.contributor.author.fl_str_mv Jiang,Yina
Hu,Yanlin
Wang,Biao
Wu,Tianlong
dc.subject.por.fl_str_mv Bivalent RNA interference
Flavanone 3-hydroxylase
Flavone synthase II
Isoflavone biosynthesis
Soybean
topic Bivalent RNA interference
Flavanone 3-hydroxylase
Flavone synthase II
Isoflavone biosynthesis
Soybean
description In this work, a bivalent RNA interference (RNAi) plant-transformation vector was constructed to silence both the flavanone 3-hydroxylase (F3H) gene and the flavone synthase II (GmFNSII) gene in soybean (Glycine max). Two further unit RNAi vectors were constructed for each of these two genes. RNAi-mediated suppression of these genes effectively regulated flavone and isoflavone production in hairy roots that arose from soybean cotyledons transformed with Agrobacterium rhizogenes ATCC15834. Notably, the bivalent RNAi vector had a significantly higher effect for increasing isoflavone production compared with the two unit RNAi vectors. The study highlighted molecular methods that could be used to enhance isoflavone production in soybean and demonstrated the challenges associated with such metabolic engineering for the production of plant natural products.
publishDate 2014
dc.date.none.fl_str_mv 2014-04-01
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132014000200002
url http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132014000200002
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv 10.1590/S1516-89132013005000018
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv text/html
dc.publisher.none.fl_str_mv Instituto de Tecnologia do Paraná - Tecpar
publisher.none.fl_str_mv Instituto de Tecnologia do Paraná - Tecpar
dc.source.none.fl_str_mv Brazilian Archives of Biology and Technology v.57 n.2 2014
reponame:Brazilian Archives of Biology and Technology
instname:Instituto de Tecnologia do Paraná (Tecpar)
instacron:TECPAR
instname_str Instituto de Tecnologia do Paraná (Tecpar)
instacron_str TECPAR
institution TECPAR
reponame_str Brazilian Archives of Biology and Technology
collection Brazilian Archives of Biology and Technology
repository.name.fl_str_mv Brazilian Archives of Biology and Technology - Instituto de Tecnologia do Paraná (Tecpar)
repository.mail.fl_str_mv babt@tecpar.br||babt@tecpar.br
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