Avaliação da genotoxicidade de resinas acrílicas de uso odontológico por meio do teste de micronúcleo em células esfoliadas da mucosa bucal
Autor(a) principal: | |
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Data de Publicação: | 2019 |
Tipo de documento: | Tese |
Idioma: | por |
Título da fonte: | Biblioteca Digital de Teses e Dissertações da UEFS |
Texto Completo: | http://tede2.uefs.br:8080/handle/tede/819 |
Resumo: | Acrylic resins are widely used in dentistry as both restorative and auxiliary materials. However, little is known about their genotoxicity. Thus, the aim of this work was to evaluate the genotoxic potential of acrylic resins used in dentistry with the Micronucleus Test in exfoliated buccal mucosal cells. In addition to the occurrence of micronuclei, degenerative nuclear alterations indicative of apoptosis (pycnose, karyorrhexis, and condensed chromatin) and necrosis (karyolysis, additionally) were investigated in cells of two distinct groups of individuals: 1) occupationally exposed professionals; 2) children and adolescents using orthodontic appliances made with acrylic resins. For evaluation in the group of exposed professionals, exfoliated cheek cells from 60 individuals of both sexes were equally divided into 3 groups: professionals exposed to acrylic resins, professionals non-exposed to acrylic resins and non-dental professionals. For evaluation in the group of children and adolescents, exfoliated cells from the cheek and palate mucosa of 30 individuals of both sexes in the age group of 6-12 years were evaluated. Collections were performed before the installation of appliances and after a period of 15 to 21 days. Cytological analyses were blindly performed under optical microscopy according to protocols suggested by Tolbert; Shy; Allen (1991, 1992) and Thomas et al. (2009). A minimum of 2,000 cells per region collected for each individual was considered for analysis. Regarding occupational exposure, no difference was observed between groups regarding the occurrence of micronuclei (p> 0.05) and karyolysis (p> 0.05). The isolated evaluation of alterations indicative of apoptosis revealed that karyorrhexis, condensed chromatin and pycnose were significantly more frequent among exposed professionals when compared to non-dental professionals, but no differences were observed when compared to non-exposed professionals. These, however, differed from non-dental professionals only for the occurrence of karyorrhexis. The combined evaluation of these changes revealed differences between groups of exposed and non-exposed professionals compared to non-dental professionals. Regarding the evaluation of children and adolescents using orthodontic appliances, no difference was observed between the two moments regarding the occurrence of micronuclei and degenerative nuclear alterations indicative of apoptosis and necrosis in cheek cells (p> 0.05). The evaluation of palate cells revealed a significant increase in the occurrence of micronuclei and nuclear alterations indicative of apoptosis (p <0.01), but not of karyolysis (p> 0.05). It could be concluded that professionals are exposed to agents capable of causing genotoxicity expressed by the increase in nuclear alteration rates indicative of apoptosis. Occupational exposure to acrylic resin monomers could a priori contribute to increased apoptosis, but the results obtained here do not allow such a conclusion, since non-exposed professionals did not differ from exposed ones. In addition, direct contact with orthodontic appliances induces an increase in the occurrence of chromosomal damage and degenerative nuclear alterations. It is important to consider that in addition to methylmethacrylate, the main monomer of acrylic resins, it is possible that local trauma caused by the devices may have stimulated the cell renewal process, interfering with differences observed. The results obtained encourage the conduction of further studies to evaluate the real genotoxic potential of acrylic resins |
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Cerqueira, Eneida de Moraes Marcilio04104455504http://lattes.cnpq.br/151785528982540898838024553http://lattes.cnpq.br/5946156533134861Cruz, João Pedro Pedrosa2019-07-30T22:58:51Z2019-05-27CRUZ, João Pedro Pedrosa. Avaliação da genotoxicidade de resinas acrílicas de uso odontológico por meio do teste de micronúcleo em células esfoliadas da mucosa bucal, 2019. 122f. Tese (Doutorado Acadêmico em Biotecnologia) - Universidade Estadual de Feira de Santana, Feira de Santana, 2019.http://tede2.uefs.br:8080/handle/tede/819Acrylic resins are widely used in dentistry as both restorative and auxiliary materials. However, little is known about their genotoxicity. Thus, the aim of this work was to evaluate the genotoxic potential of acrylic resins used in dentistry with the Micronucleus Test in exfoliated buccal mucosal cells. In addition to the occurrence of micronuclei, degenerative nuclear alterations indicative of apoptosis (pycnose, karyorrhexis, and condensed chromatin) and necrosis (karyolysis, additionally) were investigated in cells of two distinct groups of individuals: 1) occupationally exposed professionals; 2) children and adolescents using orthodontic appliances made with acrylic resins. For evaluation in the group of exposed professionals, exfoliated cheek cells from 60 individuals of both sexes were equally divided into 3 groups: professionals exposed to acrylic resins, professionals non-exposed to acrylic resins and non-dental professionals. For evaluation in the group of children and adolescents, exfoliated cells from the cheek and palate mucosa of 30 individuals of both sexes in the age group of 6-12 years were evaluated. Collections were performed before the installation of appliances and after a period of 15 to 21 days. Cytological analyses were blindly performed under optical microscopy according to protocols suggested by Tolbert; Shy; Allen (1991, 1992) and Thomas et al. (2009). A minimum of 2,000 cells per region collected for each individual was considered for analysis. Regarding occupational exposure, no difference was observed between groups regarding the occurrence of micronuclei (p> 0.05) and karyolysis (p> 0.05). The isolated evaluation of alterations indicative of apoptosis revealed that karyorrhexis, condensed chromatin and pycnose were significantly more frequent among exposed professionals when compared to non-dental professionals, but no differences were observed when compared to non-exposed professionals. These, however, differed from non-dental professionals only for the occurrence of karyorrhexis. The combined evaluation of these changes revealed differences between groups of exposed and non-exposed professionals compared to non-dental professionals. Regarding the evaluation of children and adolescents using orthodontic appliances, no difference was observed between the two moments regarding the occurrence of micronuclei and degenerative nuclear alterations indicative of apoptosis and necrosis in cheek cells (p> 0.05). The evaluation of palate cells revealed a significant increase in the occurrence of micronuclei and nuclear alterations indicative of apoptosis (p <0.01), but not of karyolysis (p> 0.05). It could be concluded that professionals are exposed to agents capable of causing genotoxicity expressed by the increase in nuclear alteration rates indicative of apoptosis. Occupational exposure to acrylic resin monomers could a priori contribute to increased apoptosis, but the results obtained here do not allow such a conclusion, since non-exposed professionals did not differ from exposed ones. In addition, direct contact with orthodontic appliances induces an increase in the occurrence of chromosomal damage and degenerative nuclear alterations. It is important to consider that in addition to methylmethacrylate, the main monomer of acrylic resins, it is possible that local trauma caused by the devices may have stimulated the cell renewal process, interfering with differences observed. The results obtained encourage the conduction of further studies to evaluate the real genotoxic potential of acrylic resinsAs resinas acrílicas são amplamente utilizadas em odontologia tanto como materiais restauradores quanto auxiliares. Pouco é conhecido, no entanto, sobre sua genotoxicidade. Assim, o objetivo deste trabalho foi avaliar, com o emprego do Teste de Micronúcleo em células esfoliadas da mucosa bucal, o potencial genotóxico de resinas acrílicas utilizadas em odontologia. Foi investigada, além da ocorrência de micronúcleos, alterações nucleares degenerativas indicadoras de apoptose (picnose, cariorréxis e cromatina condensada) e necrose (cariólise, adicionalmente), em células de dois grupos distintos de indivíduos: 1) profissionais ocupacionalmente expostos e; 2) crianças e adolescentes em uso de aparelhos ortodônticos confeccionados com resinas acrílicas. Para avaliação no grupo de profissionais expostos, foram analisadas células esfoliadas da bochecha de 60 indivíduos, de ambos os sexos, divididos igualmente em 3 grupos: profissionais expostos às resinas acrílicas, profissionais não expostos e não profissionais da odontologia. E para avaliação no grupo de crianças e adolescentes foram analisadas células esfoliadas das mucosas da bochecha e do palato de 30 destes indivíduos, de ambos os sexos, na faixa etária entre seis e 12 anos de idade. As coletas foram realizadas antes da instalação dos aparelhos e após um período de 15 a 21 dias. As análises citológicas foram feitas, em teste cego, sob microscopia óptica, segundo os protocolos sugeridos por Tolbert; Shy; Allen (1991; 1992) e Thomas et al. (2009). Um mínimo de 2.000 células por região coletada para cada indivíduo foi considerado para análise. Em relação à exposição ocupacional, não foi observada diferença entre os grupos quanto à ocorrência de micronúcleos (p>0,05) e cariólise (p>0,05). A avaliação isolada das alterações indicativas de apoptose revelou que cariorréxis, cromatina condensada e picnose foram de ocorrência significativamente maior entre os profissionais expostos quando comparados aos não profissionais, mas não foram observadas diferenças quando comparados aos profissionais não expostos. Estes, contudo, diferiram dos não profissionais apenas na ocorrência de cariorréxis. A avaliação conjunta destas alterações revelou, no entanto, diferenças entre os grupos de profissionais expostos e não expostos comparativamente aos não profissionais. Quanto à avaliação das crianças e adolescentes em uso de aparelhos ortodônticos, não foi observada diferença entre os dois momentos quanto à ocorrência de micronúcleos e de alterações nucleares degenerativas indicativas de apoptose e necrose nas células da bochecha (p>0,05). A avaliação das células do palato revelou aumento significativo na ocorrência de micronúcleos e de alterações nucleares indicativas de apoptose (p<0,01), mas não de cariólise (p>0,05). É possível concluir que os profissionais estão expostos a agentes capazes de provocar genotoxicidade expressa pelo aumento nas taxas de alterações nucleares indicativas de apoptose. A exposição ocupacional aos monômeros das resinas acrílicas poderia a priori contribuir para o aumento da apoptose, mas os resultados aqui obtidos não permitem tal afirmação, vez que profissionais não expostos não diferiram dos expostos. Além disso, foi possível observar, no caso das crianças e adolescentes, que o contato direto com os aparelhos ortodônticos induz aumento na ocorrência de danos cromossômicos e de alterações nucleares degenerativas. É importante considerar que, adicionalmente ao metilmetacrilato, principal monômero das resinas acrílicas, é possível que tenha havido trauma local provocado pelos aparelhos, o que pode ter estimulado o processo de renovação celular, interferindo nas diferenças observadas. Os resultados encontrados suscitam a realização de novos estudos para avaliação do real potencial genotóxico das resinas acrílicasSubmitted by Verena Pereira (verenagoncalves@uefs.br) on 2019-07-30T22:58:51Z No. of bitstreams: 1 Tese_genotoxicidade_resinas_acrlicas.pdf: 30747883 bytes, checksum: 09bfcbc6315eb2333eb22eb53d7ea6de (MD5)Made available in DSpace on 2019-07-30T22:58:51Z (GMT). No. of bitstreams: 1 Tese_genotoxicidade_resinas_acrlicas.pdf: 30747883 bytes, checksum: 09bfcbc6315eb2333eb22eb53d7ea6de (MD5) Previous issue date: 2019-05-27application/pdfporUniversidade Estadual de Feira de SantanaDoutorado Acadêmico em BiotecnologiaUEFSBrasilDEPARTAMENTO DE SAÚDEresinas acrílicasgenotoxicidademicronúcleodanos ao DNAapoptoseacrylic resinsgennotoxicitymicronucleus testDNA damageapoptosisCIENCIAS DA SAUDE::ODONTOLOGIAAvaliação da genotoxicidade de resinas acrílicas de uso odontológico por meio do teste de micronúcleo em células esfoliadas da mucosa bucalinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/doctoralThesis-68152692297897915436006006001006395569471477890-2070498469879244349info:eu-repo/semantics/openAccessreponame:Biblioteca Digital de Teses e Dissertações da UEFSinstname:Universidade Estadual de Feira de Santana (UEFS)instacron:UEFSORIGINALTese_genotoxicidade_resinas_acrlicas.pdfTese_genotoxicidade_resinas_acrlicas.pdfapplication/pdf30747883http://tede2.uefs.br:8080/bitstream/tede/819/2/Tese_genotoxicidade_resinas_acrlicas.pdf09bfcbc6315eb2333eb22eb53d7ea6deMD52LICENSElicense.txtlicense.txttext/plain; charset=utf-82089http://tede2.uefs.br:8080/bitstream/tede/819/1/license.txt7b5ba3d2445355f386edab96125d42b7MD51tede/8192019-07-30 19:58:51.189oai:tede2.uefs.br:8080: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Biblioteca Digital de Teses e Dissertaçõeshttp://tede2.uefs.br:8080/PUBhttp://tede2.uefs.br:8080/oai/requestbcuefs@uefs.br|| bcref@uefs.br||bcuefs@uefs.bropendoar:2019-07-30T22:58:51Biblioteca Digital de Teses e Dissertações da UEFS - Universidade Estadual de Feira de Santana (UEFS)false |
dc.title.por.fl_str_mv |
Avaliação da genotoxicidade de resinas acrílicas de uso odontológico por meio do teste de micronúcleo em células esfoliadas da mucosa bucal |
title |
Avaliação da genotoxicidade de resinas acrílicas de uso odontológico por meio do teste de micronúcleo em células esfoliadas da mucosa bucal |
spellingShingle |
Avaliação da genotoxicidade de resinas acrílicas de uso odontológico por meio do teste de micronúcleo em células esfoliadas da mucosa bucal Cruz, João Pedro Pedrosa resinas acrílicas genotoxicidade micronúcleo danos ao DNA apoptose acrylic resins gennotoxicity micronucleus test DNA damage apoptosis CIENCIAS DA SAUDE::ODONTOLOGIA |
title_short |
Avaliação da genotoxicidade de resinas acrílicas de uso odontológico por meio do teste de micronúcleo em células esfoliadas da mucosa bucal |
title_full |
Avaliação da genotoxicidade de resinas acrílicas de uso odontológico por meio do teste de micronúcleo em células esfoliadas da mucosa bucal |
title_fullStr |
Avaliação da genotoxicidade de resinas acrílicas de uso odontológico por meio do teste de micronúcleo em células esfoliadas da mucosa bucal |
title_full_unstemmed |
Avaliação da genotoxicidade de resinas acrílicas de uso odontológico por meio do teste de micronúcleo em células esfoliadas da mucosa bucal |
title_sort |
Avaliação da genotoxicidade de resinas acrílicas de uso odontológico por meio do teste de micronúcleo em células esfoliadas da mucosa bucal |
author |
Cruz, João Pedro Pedrosa |
author_facet |
Cruz, João Pedro Pedrosa |
author_role |
author |
dc.contributor.advisor1.fl_str_mv |
Cerqueira, Eneida de Moraes Marcilio |
dc.contributor.advisor1ID.fl_str_mv |
04104455504 |
dc.contributor.advisor1Lattes.fl_str_mv |
http://lattes.cnpq.br/1517855289825408 |
dc.contributor.authorID.fl_str_mv |
98838024553 |
dc.contributor.authorLattes.fl_str_mv |
http://lattes.cnpq.br/5946156533134861 |
dc.contributor.author.fl_str_mv |
Cruz, João Pedro Pedrosa |
contributor_str_mv |
Cerqueira, Eneida de Moraes Marcilio |
dc.subject.por.fl_str_mv |
resinas acrílicas genotoxicidade micronúcleo danos ao DNA apoptose |
topic |
resinas acrílicas genotoxicidade micronúcleo danos ao DNA apoptose acrylic resins gennotoxicity micronucleus test DNA damage apoptosis CIENCIAS DA SAUDE::ODONTOLOGIA |
dc.subject.eng.fl_str_mv |
acrylic resins gennotoxicity micronucleus test DNA damage apoptosis |
dc.subject.cnpq.fl_str_mv |
CIENCIAS DA SAUDE::ODONTOLOGIA |
description |
Acrylic resins are widely used in dentistry as both restorative and auxiliary materials. However, little is known about their genotoxicity. Thus, the aim of this work was to evaluate the genotoxic potential of acrylic resins used in dentistry with the Micronucleus Test in exfoliated buccal mucosal cells. In addition to the occurrence of micronuclei, degenerative nuclear alterations indicative of apoptosis (pycnose, karyorrhexis, and condensed chromatin) and necrosis (karyolysis, additionally) were investigated in cells of two distinct groups of individuals: 1) occupationally exposed professionals; 2) children and adolescents using orthodontic appliances made with acrylic resins. For evaluation in the group of exposed professionals, exfoliated cheek cells from 60 individuals of both sexes were equally divided into 3 groups: professionals exposed to acrylic resins, professionals non-exposed to acrylic resins and non-dental professionals. For evaluation in the group of children and adolescents, exfoliated cells from the cheek and palate mucosa of 30 individuals of both sexes in the age group of 6-12 years were evaluated. Collections were performed before the installation of appliances and after a period of 15 to 21 days. Cytological analyses were blindly performed under optical microscopy according to protocols suggested by Tolbert; Shy; Allen (1991, 1992) and Thomas et al. (2009). A minimum of 2,000 cells per region collected for each individual was considered for analysis. Regarding occupational exposure, no difference was observed between groups regarding the occurrence of micronuclei (p> 0.05) and karyolysis (p> 0.05). The isolated evaluation of alterations indicative of apoptosis revealed that karyorrhexis, condensed chromatin and pycnose were significantly more frequent among exposed professionals when compared to non-dental professionals, but no differences were observed when compared to non-exposed professionals. These, however, differed from non-dental professionals only for the occurrence of karyorrhexis. The combined evaluation of these changes revealed differences between groups of exposed and non-exposed professionals compared to non-dental professionals. Regarding the evaluation of children and adolescents using orthodontic appliances, no difference was observed between the two moments regarding the occurrence of micronuclei and degenerative nuclear alterations indicative of apoptosis and necrosis in cheek cells (p> 0.05). The evaluation of palate cells revealed a significant increase in the occurrence of micronuclei and nuclear alterations indicative of apoptosis (p <0.01), but not of karyolysis (p> 0.05). It could be concluded that professionals are exposed to agents capable of causing genotoxicity expressed by the increase in nuclear alteration rates indicative of apoptosis. Occupational exposure to acrylic resin monomers could a priori contribute to increased apoptosis, but the results obtained here do not allow such a conclusion, since non-exposed professionals did not differ from exposed ones. In addition, direct contact with orthodontic appliances induces an increase in the occurrence of chromosomal damage and degenerative nuclear alterations. It is important to consider that in addition to methylmethacrylate, the main monomer of acrylic resins, it is possible that local trauma caused by the devices may have stimulated the cell renewal process, interfering with differences observed. The results obtained encourage the conduction of further studies to evaluate the real genotoxic potential of acrylic resins |
publishDate |
2019 |
dc.date.accessioned.fl_str_mv |
2019-07-30T22:58:51Z |
dc.date.issued.fl_str_mv |
2019-05-27 |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/doctoralThesis |
format |
doctoralThesis |
status_str |
publishedVersion |
dc.identifier.citation.fl_str_mv |
CRUZ, João Pedro Pedrosa. Avaliação da genotoxicidade de resinas acrílicas de uso odontológico por meio do teste de micronúcleo em células esfoliadas da mucosa bucal, 2019. 122f. Tese (Doutorado Acadêmico em Biotecnologia) - Universidade Estadual de Feira de Santana, Feira de Santana, 2019. |
dc.identifier.uri.fl_str_mv |
http://tede2.uefs.br:8080/handle/tede/819 |
identifier_str_mv |
CRUZ, João Pedro Pedrosa. Avaliação da genotoxicidade de resinas acrílicas de uso odontológico por meio do teste de micronúcleo em células esfoliadas da mucosa bucal, 2019. 122f. Tese (Doutorado Acadêmico em Biotecnologia) - Universidade Estadual de Feira de Santana, Feira de Santana, 2019. |
url |
http://tede2.uefs.br:8080/handle/tede/819 |
dc.language.iso.fl_str_mv |
por |
language |
por |
dc.relation.program.fl_str_mv |
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dc.relation.confidence.fl_str_mv |
600 600 600 |
dc.relation.department.fl_str_mv |
1006395569471477890 |
dc.relation.cnpq.fl_str_mv |
-2070498469879244349 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
application/pdf |
dc.publisher.none.fl_str_mv |
Universidade Estadual de Feira de Santana |
dc.publisher.program.fl_str_mv |
Doutorado Acadêmico em Biotecnologia |
dc.publisher.initials.fl_str_mv |
UEFS |
dc.publisher.country.fl_str_mv |
Brasil |
dc.publisher.department.fl_str_mv |
DEPARTAMENTO DE SAÚDE |
publisher.none.fl_str_mv |
Universidade Estadual de Feira de Santana |
dc.source.none.fl_str_mv |
reponame:Biblioteca Digital de Teses e Dissertações da UEFS instname:Universidade Estadual de Feira de Santana (UEFS) instacron:UEFS |
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Universidade Estadual de Feira de Santana (UEFS) |
instacron_str |
UEFS |
institution |
UEFS |
reponame_str |
Biblioteca Digital de Teses e Dissertações da UEFS |
collection |
Biblioteca Digital de Teses e Dissertações da UEFS |
bitstream.url.fl_str_mv |
http://tede2.uefs.br:8080/bitstream/tede/819/2/Tese_genotoxicidade_resinas_acrlicas.pdf http://tede2.uefs.br:8080/bitstream/tede/819/1/license.txt |
bitstream.checksum.fl_str_mv |
09bfcbc6315eb2333eb22eb53d7ea6de 7b5ba3d2445355f386edab96125d42b7 |
bitstream.checksumAlgorithm.fl_str_mv |
MD5 MD5 |
repository.name.fl_str_mv |
Biblioteca Digital de Teses e Dissertações da UEFS - Universidade Estadual de Feira de Santana (UEFS) |
repository.mail.fl_str_mv |
bcuefs@uefs.br|| bcref@uefs.br||bcuefs@uefs.br |
_version_ |
1809288776899362816 |