Molecular Identification of Leptospira interrogans in Naturally Infected Cows from a Rural Property in the Border Region - Case Report

Detalhes bibliográficos
Autor(a) principal: Pastre, Giovani Batista
Data de Publicação: 2020
Outros Autores: Santos, Isabela Carvalho dos, Delai, Robson Michael, Mota, Edinalva Madalena de Almeida, Barbosa, Lidiane Nunes, Chideroli, Roberta Torres, Pretto-Giordano, Lucienne Garcia, Alberton, Luiz Rômulo, Pereira, Ulisses de Pádua, Gonçalves, Daniela Dib
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Semina. Ciências Agrárias (Online)
Texto Completo: https://ojs.uel.br/revistas/uel/index.php/semagrarias/article/view/37575
Resumo: The aim of this study was to investigate the antibodies and DNA of Leptospira spp. isolated from infected cattle in a small rural dairy farm in a border region between Brazil and Paraguay. Blood and urine samples were collected from 50 Holstein cows aged between 1 and 15 years. The diagnostic tests performed were microscopic serum agglutination for antibody detection and polymerase chain reaction for Leptospira spp. detection. Out of the samples analyzed, 48% were MAT positive with titers ranging from 100 to 400, and the most prevalent antibody was to the serovar Hardjo. One serum sample was amplified to 549 bp for the sec y gene, and sequencing identified it as L. interrogans. This is the first report from northwestern Paraná (PR) State of L. interrogans identification in naturally infected milk cattle. Thus, based on these results, to enhance production efficiency, new serological and molecular studies on dairy cattle from border regions are required to characterize the epidemiology of possible genotypes and their consequences in affected herds.
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spelling Molecular Identification of Leptospira interrogans in Naturally Infected Cows from a Rural Property in the Border Region - Case ReportIdentificação molecular de Leptospira interrogans em Vacas naturalmente infectadas de uma propriedade rural em Região Fronteiriça - Relato de casoSpirochetesDiagnosisLeptospirosisRuminantsOne Health.EspiroquetasDiagnósticoLeptospiroseRuminantesSaúde Única.The aim of this study was to investigate the antibodies and DNA of Leptospira spp. isolated from infected cattle in a small rural dairy farm in a border region between Brazil and Paraguay. Blood and urine samples were collected from 50 Holstein cows aged between 1 and 15 years. The diagnostic tests performed were microscopic serum agglutination for antibody detection and polymerase chain reaction for Leptospira spp. detection. Out of the samples analyzed, 48% were MAT positive with titers ranging from 100 to 400, and the most prevalent antibody was to the serovar Hardjo. One serum sample was amplified to 549 bp for the sec y gene, and sequencing identified it as L. interrogans. This is the first report from northwestern Paraná (PR) State of L. interrogans identification in naturally infected milk cattle. Thus, based on these results, to enhance production efficiency, new serological and molecular studies on dairy cattle from border regions are required to characterize the epidemiology of possible genotypes and their consequences in affected herds.O objetivo deste trabalho foi pesquisar anticorpos e DNA de Leptospira spp. em uma propriedade rural leiteira de uma região fronteiriça entre Brasil e Paraguai. Foram coletadas amostras de sangue e urina de 50 animais da raça Holandesa com idade variando de um a quinze anos, de uma pequena propriedade rural de exploração leiteira em uma região de fronteira entre Brasil e Paraguai. Quanto aos diferentes diagnósticos foi utilizado a soroaglutinação microscópica para a detecção de anticorpos e também foi realizada a reação em cadeia pela polimerase para a detecção de DNA de Leptospira spp. Das amostras analisadas, 48,00% foram soro reagentes na SAM com títulos variando de 100 a 400 e o anticorpo contra o sorovar mais prevalente foi o Hardjo. Uma amostra de soro amplificou 549pb para o gene sec y, e no sequenciamento foi identificada como Leptospira interrogans. Este é o primeiro relato da região noroeste do estado do Paraná (PR) relacionado à identificação de L. interrogans de bovinos de leite naturalmente infectados e em virtude dos resultados deste trabalho são necessários novos estudos sorológicos e moleculares em criações de gado de leite de regiões fronteiriças para se caracterizar a epidemiologia dos possíveis genótipos e suas possíveis consequências nos rebanhos afetados visando sempre à eficiência da produção.UEL2020-05-13info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionapplication/pdfhttps://ojs.uel.br/revistas/uel/index.php/semagrarias/article/view/3757510.5433/1679-0359.2020v41n4p1433Semina: Ciências Agrárias; Vol. 41 No. 4 (2020); 1433-1438Semina: Ciências Agrárias; v. 41 n. 4 (2020); 1433-14381679-03591676-546Xreponame:Semina. Ciências Agrárias (Online)instname:Universidade Estadual de Londrina (UEL)instacron:UELenghttps://ojs.uel.br/revistas/uel/index.php/semagrarias/article/view/37575/27363Copyright (c) 2020 Semina: Ciências Agráriashttp://creativecommons.org/licenses/by-nc/4.0info:eu-repo/semantics/openAccessPastre, Giovani BatistaSantos, Isabela Carvalho dosDelai, Robson MichaelMota, Edinalva Madalena de AlmeidaBarbosa, Lidiane NunesChideroli, Roberta TorresPretto-Giordano, Lucienne GarciaAlberton, Luiz RômuloPereira, Ulisses de PáduaGonçalves, Daniela Dib2022-10-07T16:52:49Zoai:ojs.pkp.sfu.ca:article/37575Revistahttp://www.uel.br/revistas/uel/index.php/semagrariasPUBhttps://ojs.uel.br/revistas/uel/index.php/semagrarias/oaisemina.agrarias@uel.br1679-03591676-546Xopendoar:2022-10-07T16:52:49Semina. Ciências Agrárias (Online) - Universidade Estadual de Londrina (UEL)false
dc.title.none.fl_str_mv Molecular Identification of Leptospira interrogans in Naturally Infected Cows from a Rural Property in the Border Region - Case Report
Identificação molecular de Leptospira interrogans em Vacas naturalmente infectadas de uma propriedade rural em Região Fronteiriça - Relato de caso
title Molecular Identification of Leptospira interrogans in Naturally Infected Cows from a Rural Property in the Border Region - Case Report
spellingShingle Molecular Identification of Leptospira interrogans in Naturally Infected Cows from a Rural Property in the Border Region - Case Report
Pastre, Giovani Batista
Spirochetes
Diagnosis
Leptospirosis
Ruminants
One Health.
Espiroquetas
Diagnóstico
Leptospirose
Ruminantes
Saúde Única.
title_short Molecular Identification of Leptospira interrogans in Naturally Infected Cows from a Rural Property in the Border Region - Case Report
title_full Molecular Identification of Leptospira interrogans in Naturally Infected Cows from a Rural Property in the Border Region - Case Report
title_fullStr Molecular Identification of Leptospira interrogans in Naturally Infected Cows from a Rural Property in the Border Region - Case Report
title_full_unstemmed Molecular Identification of Leptospira interrogans in Naturally Infected Cows from a Rural Property in the Border Region - Case Report
title_sort Molecular Identification of Leptospira interrogans in Naturally Infected Cows from a Rural Property in the Border Region - Case Report
author Pastre, Giovani Batista
author_facet Pastre, Giovani Batista
Santos, Isabela Carvalho dos
Delai, Robson Michael
Mota, Edinalva Madalena de Almeida
Barbosa, Lidiane Nunes
Chideroli, Roberta Torres
Pretto-Giordano, Lucienne Garcia
Alberton, Luiz Rômulo
Pereira, Ulisses de Pádua
Gonçalves, Daniela Dib
author_role author
author2 Santos, Isabela Carvalho dos
Delai, Robson Michael
Mota, Edinalva Madalena de Almeida
Barbosa, Lidiane Nunes
Chideroli, Roberta Torres
Pretto-Giordano, Lucienne Garcia
Alberton, Luiz Rômulo
Pereira, Ulisses de Pádua
Gonçalves, Daniela Dib
author2_role author
author
author
author
author
author
author
author
author
dc.contributor.author.fl_str_mv Pastre, Giovani Batista
Santos, Isabela Carvalho dos
Delai, Robson Michael
Mota, Edinalva Madalena de Almeida
Barbosa, Lidiane Nunes
Chideroli, Roberta Torres
Pretto-Giordano, Lucienne Garcia
Alberton, Luiz Rômulo
Pereira, Ulisses de Pádua
Gonçalves, Daniela Dib
dc.subject.por.fl_str_mv Spirochetes
Diagnosis
Leptospirosis
Ruminants
One Health.
Espiroquetas
Diagnóstico
Leptospirose
Ruminantes
Saúde Única.
topic Spirochetes
Diagnosis
Leptospirosis
Ruminants
One Health.
Espiroquetas
Diagnóstico
Leptospirose
Ruminantes
Saúde Única.
description The aim of this study was to investigate the antibodies and DNA of Leptospira spp. isolated from infected cattle in a small rural dairy farm in a border region between Brazil and Paraguay. Blood and urine samples were collected from 50 Holstein cows aged between 1 and 15 years. The diagnostic tests performed were microscopic serum agglutination for antibody detection and polymerase chain reaction for Leptospira spp. detection. Out of the samples analyzed, 48% were MAT positive with titers ranging from 100 to 400, and the most prevalent antibody was to the serovar Hardjo. One serum sample was amplified to 549 bp for the sec y gene, and sequencing identified it as L. interrogans. This is the first report from northwestern Paraná (PR) State of L. interrogans identification in naturally infected milk cattle. Thus, based on these results, to enhance production efficiency, new serological and molecular studies on dairy cattle from border regions are required to characterize the epidemiology of possible genotypes and their consequences in affected herds.
publishDate 2020
dc.date.none.fl_str_mv 2020-05-13
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv https://ojs.uel.br/revistas/uel/index.php/semagrarias/article/view/37575
10.5433/1679-0359.2020v41n4p1433
url https://ojs.uel.br/revistas/uel/index.php/semagrarias/article/view/37575
identifier_str_mv 10.5433/1679-0359.2020v41n4p1433
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv https://ojs.uel.br/revistas/uel/index.php/semagrarias/article/view/37575/27363
dc.rights.driver.fl_str_mv Copyright (c) 2020 Semina: Ciências Agrárias
http://creativecommons.org/licenses/by-nc/4.0
info:eu-repo/semantics/openAccess
rights_invalid_str_mv Copyright (c) 2020 Semina: Ciências Agrárias
http://creativecommons.org/licenses/by-nc/4.0
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv UEL
publisher.none.fl_str_mv UEL
dc.source.none.fl_str_mv Semina: Ciências Agrárias; Vol. 41 No. 4 (2020); 1433-1438
Semina: Ciências Agrárias; v. 41 n. 4 (2020); 1433-1438
1679-0359
1676-546X
reponame:Semina. Ciências Agrárias (Online)
instname:Universidade Estadual de Londrina (UEL)
instacron:UEL
instname_str Universidade Estadual de Londrina (UEL)
instacron_str UEL
institution UEL
reponame_str Semina. Ciências Agrárias (Online)
collection Semina. Ciências Agrárias (Online)
repository.name.fl_str_mv Semina. Ciências Agrárias (Online) - Universidade Estadual de Londrina (UEL)
repository.mail.fl_str_mv semina.agrarias@uel.br
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