Genetic profiles of Mycobacterium bovis from a cattle herd in southernmost Brazil
Autor(a) principal: | |
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Data de Publicação: | 2016 |
Outros Autores: | , , , , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Semina. Ciências Agrárias (Online) |
Texto Completo: | https://ojs.uel.br/revistas/uel/index.php/semagrarias/article/view/27318 |
Resumo: | Mycobacterium bovis is the agent of bovine tuberculosis, a disease endemic to all Brazilian states. Molecular typing techniques help to stratify and refine data, providing information that facilitates epidemiological research. In this study, MIRU-VNTR, targeting 24 loci, was employed to identify and characterize the genetic groups of M. bovis isolates obtained from an outbreak of bovine tuberculosis. Eighteen acid-fast bacilli isolates, obtained from bovine tissue samples, and reactive to the comparative cervical tuberculin test, were identified as species of the M. tuberculosis complex, and were genotyped by MIRU-VNTR with 24 primer pairs. Genotyping revealed three genetic profiles comprising one with 15 isolates (83.3%), one with two isolates (11.1%), and one profile with one unique isolate (5.6%). This distinction was achieved with the MIRU 31 primer, which resulted in clustering of two isolates into the same profile, and ETR A, B, and C, which discriminated the isolate with a unique profile. The occurrence of clustered isolates is indicative of recent transmission, whereas isolates with a unique profile suggest reactivation of latent infection. The presence of different M. bovis genotypes in the same herd suggests movement of infected animals or different sources of intra-herd infection. Use of the MIRU-VNTR molecular epidemiology technique in M. bovis isolates obtained from an outbreak of bovine tuberculosis in Rio Grande do Sul state demonstrated the genetic diversity of circulating strains, despite the presence of a predominant group. |
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Semina. Ciências Agrárias (Online) |
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Genetic profiles of Mycobacterium bovis from a cattle herd in southernmost BrazilPerfis genéticos de Mycobacterium bovis em um rebanho bovino no extremo sul do BrasilBovine tuberculosisGenetic diversityMIRU-VNTRMycobacterium bovis.Diversidade genéticaTuberculose bovinaMIRU-VNTRMycobacterium bovis.Mycobacterium bovis is the agent of bovine tuberculosis, a disease endemic to all Brazilian states. Molecular typing techniques help to stratify and refine data, providing information that facilitates epidemiological research. In this study, MIRU-VNTR, targeting 24 loci, was employed to identify and characterize the genetic groups of M. bovis isolates obtained from an outbreak of bovine tuberculosis. Eighteen acid-fast bacilli isolates, obtained from bovine tissue samples, and reactive to the comparative cervical tuberculin test, were identified as species of the M. tuberculosis complex, and were genotyped by MIRU-VNTR with 24 primer pairs. Genotyping revealed three genetic profiles comprising one with 15 isolates (83.3%), one with two isolates (11.1%), and one profile with one unique isolate (5.6%). This distinction was achieved with the MIRU 31 primer, which resulted in clustering of two isolates into the same profile, and ETR A, B, and C, which discriminated the isolate with a unique profile. The occurrence of clustered isolates is indicative of recent transmission, whereas isolates with a unique profile suggest reactivation of latent infection. The presence of different M. bovis genotypes in the same herd suggests movement of infected animals or different sources of intra-herd infection. Use of the MIRU-VNTR molecular epidemiology technique in M. bovis isolates obtained from an outbreak of bovine tuberculosis in Rio Grande do Sul state demonstrated the genetic diversity of circulating strains, despite the presence of a predominant group.Mycobacterium bovis é o agente da tuberculose bovina, enfermidade endêmica diagnosticada em todos os estados brasileiros. As técnicas de tipagem molecular auxiliam a estratificar e refinar dados, fornecendo informações que facilitam as atividades epidemiológicas. Neste estudo aplicou-se a técnica MIRU-VNTR com 24 loci para caracterizar e identificar os agrupamentos genéticos de isolados de M. bovis oriundos de um foco de tuberculose bovina. Dezoito isolados de bacilos álcool-ácido resistentes, obtidos de amostras de tecido de bovinos reagentes ao teste cervical comparativo e identificados como espécies do complexo M. tuberculosis, foram genotipados por meio de MIRU-VNTR com 24 pares de iniciadores. A genotipagem revelou três perfis genéticos distintos: um para 15 isolados (83,3%); um para dois isolados (11,1%) e, por fim, um isolado com perfil único (5,6%). Os iniciadores responsáveis por essa distinção foram MIRU 31, que agrupou dois isolados em um perfil, e ETR A, B e C, que discriminaram o isolado de perfil único. A ocorrência de agrupamentos de isolados é indicativa de transmissão recente, ao passo que isolados de perfil único sugerem reativação de infecção latente. A presença de diferentes genótipos de M. bovis no mesmo rebanho sugere haver circulação de animais doentes ou existirem diferentes fontes de infecção intrarrebanho. A aplicação da técnica de epidemiologia molecular MIRU-VNTR a isolados de M. bovis obtidos a partir de um foco de tuberculose bovina no Rio Grande do Sul demonstrou haver diversidade genética entre as estirpes em circulação, embora exista um agrupamento predominante.UEL2016-11-09info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionapplication/pdfhttps://ojs.uel.br/revistas/uel/index.php/semagrarias/article/view/2731810.5433/1679-0359.2016v37n5Supl2p3719Semina: Ciências Agrárias; Vol. 37 No. 5Supl2 (2016); 3719-3726Semina: Ciências Agrárias; v. 37 n. 5Supl2 (2016); 3719-37261679-03591676-546Xreponame:Semina. Ciências Agrárias (Online)instname:Universidade Estadual de Londrina (UEL)instacron:UELenghttps://ojs.uel.br/revistas/uel/index.php/semagrarias/article/view/27318/19938Copyright (c) 2016 Semina: Ciências Agráriashttp://creativecommons.org/licenses/by-nc/4.0info:eu-repo/semantics/openAccessSouza Filho, Antonio Francisco deOsório, Ana Luiza Alves RosaJorge, Klaudia dos Santos GonçalvesAraújo, Flábio RibeiroVidal, Carlos Eugênio SotoAraújo, Cristina PiresAlbertti, Letícia Alves GomesCazola, Daniela de OliveiraFerreira Neto, José SoaresHeinemann, Marcos Bryan2022-11-29T16:58:03Zoai:ojs.pkp.sfu.ca:article/27318Revistahttp://www.uel.br/revistas/uel/index.php/semagrariasPUBhttps://ojs.uel.br/revistas/uel/index.php/semagrarias/oaisemina.agrarias@uel.br1679-03591676-546Xopendoar:2022-11-29T16:58:03Semina. Ciências Agrárias (Online) - Universidade Estadual de Londrina (UEL)false |
dc.title.none.fl_str_mv |
Genetic profiles of Mycobacterium bovis from a cattle herd in southernmost Brazil Perfis genéticos de Mycobacterium bovis em um rebanho bovino no extremo sul do Brasil |
title |
Genetic profiles of Mycobacterium bovis from a cattle herd in southernmost Brazil |
spellingShingle |
Genetic profiles of Mycobacterium bovis from a cattle herd in southernmost Brazil Souza Filho, Antonio Francisco de Bovine tuberculosis Genetic diversity MIRU-VNTR Mycobacterium bovis. Diversidade genética Tuberculose bovina MIRU-VNTR Mycobacterium bovis. |
title_short |
Genetic profiles of Mycobacterium bovis from a cattle herd in southernmost Brazil |
title_full |
Genetic profiles of Mycobacterium bovis from a cattle herd in southernmost Brazil |
title_fullStr |
Genetic profiles of Mycobacterium bovis from a cattle herd in southernmost Brazil |
title_full_unstemmed |
Genetic profiles of Mycobacterium bovis from a cattle herd in southernmost Brazil |
title_sort |
Genetic profiles of Mycobacterium bovis from a cattle herd in southernmost Brazil |
author |
Souza Filho, Antonio Francisco de |
author_facet |
Souza Filho, Antonio Francisco de Osório, Ana Luiza Alves Rosa Jorge, Klaudia dos Santos Gonçalves Araújo, Flábio Ribeiro Vidal, Carlos Eugênio Soto Araújo, Cristina Pires Albertti, Letícia Alves Gomes Cazola, Daniela de Oliveira Ferreira Neto, José Soares Heinemann, Marcos Bryan |
author_role |
author |
author2 |
Osório, Ana Luiza Alves Rosa Jorge, Klaudia dos Santos Gonçalves Araújo, Flábio Ribeiro Vidal, Carlos Eugênio Soto Araújo, Cristina Pires Albertti, Letícia Alves Gomes Cazola, Daniela de Oliveira Ferreira Neto, José Soares Heinemann, Marcos Bryan |
author2_role |
author author author author author author author author author |
dc.contributor.author.fl_str_mv |
Souza Filho, Antonio Francisco de Osório, Ana Luiza Alves Rosa Jorge, Klaudia dos Santos Gonçalves Araújo, Flábio Ribeiro Vidal, Carlos Eugênio Soto Araújo, Cristina Pires Albertti, Letícia Alves Gomes Cazola, Daniela de Oliveira Ferreira Neto, José Soares Heinemann, Marcos Bryan |
dc.subject.por.fl_str_mv |
Bovine tuberculosis Genetic diversity MIRU-VNTR Mycobacterium bovis. Diversidade genética Tuberculose bovina MIRU-VNTR Mycobacterium bovis. |
topic |
Bovine tuberculosis Genetic diversity MIRU-VNTR Mycobacterium bovis. Diversidade genética Tuberculose bovina MIRU-VNTR Mycobacterium bovis. |
description |
Mycobacterium bovis is the agent of bovine tuberculosis, a disease endemic to all Brazilian states. Molecular typing techniques help to stratify and refine data, providing information that facilitates epidemiological research. In this study, MIRU-VNTR, targeting 24 loci, was employed to identify and characterize the genetic groups of M. bovis isolates obtained from an outbreak of bovine tuberculosis. Eighteen acid-fast bacilli isolates, obtained from bovine tissue samples, and reactive to the comparative cervical tuberculin test, were identified as species of the M. tuberculosis complex, and were genotyped by MIRU-VNTR with 24 primer pairs. Genotyping revealed three genetic profiles comprising one with 15 isolates (83.3%), one with two isolates (11.1%), and one profile with one unique isolate (5.6%). This distinction was achieved with the MIRU 31 primer, which resulted in clustering of two isolates into the same profile, and ETR A, B, and C, which discriminated the isolate with a unique profile. The occurrence of clustered isolates is indicative of recent transmission, whereas isolates with a unique profile suggest reactivation of latent infection. The presence of different M. bovis genotypes in the same herd suggests movement of infected animals or different sources of intra-herd infection. Use of the MIRU-VNTR molecular epidemiology technique in M. bovis isolates obtained from an outbreak of bovine tuberculosis in Rio Grande do Sul state demonstrated the genetic diversity of circulating strains, despite the presence of a predominant group. |
publishDate |
2016 |
dc.date.none.fl_str_mv |
2016-11-09 |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
https://ojs.uel.br/revistas/uel/index.php/semagrarias/article/view/27318 10.5433/1679-0359.2016v37n5Supl2p3719 |
url |
https://ojs.uel.br/revistas/uel/index.php/semagrarias/article/view/27318 |
identifier_str_mv |
10.5433/1679-0359.2016v37n5Supl2p3719 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
https://ojs.uel.br/revistas/uel/index.php/semagrarias/article/view/27318/19938 |
dc.rights.driver.fl_str_mv |
Copyright (c) 2016 Semina: Ciências Agrárias http://creativecommons.org/licenses/by-nc/4.0 info:eu-repo/semantics/openAccess |
rights_invalid_str_mv |
Copyright (c) 2016 Semina: Ciências Agrárias http://creativecommons.org/licenses/by-nc/4.0 |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
application/pdf |
dc.publisher.none.fl_str_mv |
UEL |
publisher.none.fl_str_mv |
UEL |
dc.source.none.fl_str_mv |
Semina: Ciências Agrárias; Vol. 37 No. 5Supl2 (2016); 3719-3726 Semina: Ciências Agrárias; v. 37 n. 5Supl2 (2016); 3719-3726 1679-0359 1676-546X reponame:Semina. Ciências Agrárias (Online) instname:Universidade Estadual de Londrina (UEL) instacron:UEL |
instname_str |
Universidade Estadual de Londrina (UEL) |
instacron_str |
UEL |
institution |
UEL |
reponame_str |
Semina. Ciências Agrárias (Online) |
collection |
Semina. Ciências Agrárias (Online) |
repository.name.fl_str_mv |
Semina. Ciências Agrárias (Online) - Universidade Estadual de Londrina (UEL) |
repository.mail.fl_str_mv |
semina.agrarias@uel.br |
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1799306076938567680 |