Hematology of Nile tilapia (Oreochromis niloticus) subjected to anesthesia and anticoagulation protocols
Autor(a) principal: | |
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Data de Publicação: | 2015 |
Outros Autores: | , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Semina. Ciências Agrárias (Online) |
Texto Completo: | https://ojs.uel.br/revistas/uel/index.php/semagrarias/article/view/19829 |
Resumo: | Clinical hematology facilitates the diagnosis of disease and can act as a prognostic indicator of pathological conditions in fish. The aim of the present study was to evaluate hematological parameters of Nile tilapia (Oreochromis niloticus) subjected to different anesthetics and anticoagulants. Thirty apparently healthy fishes (average weight of 473 ± 35. 50 g and mean total length of 29. 33 ± 0. 37 cm), were selected from the local commercial fish farm in the Lages municipality (Santa Catarina, Brazil). The animals were randomly divided into three groups of 10. In two groups, anesthesia was induced with eugenol (70 mg·L- 1) (EG) and Benzocaine hydrochloride (100 mg·L-1) (BG), respectively. Anesthesia was not administered to fish of the third group (CG/control group). Blood samples were obtained by venipuncture of the caudal vessels and placed into microtubes containing sodium heparin or Na2EDTA for further analysis. The results were analyzed by Sigma Stat for Windows, the paired t-test for significant differences between anticoagulants of the same group, and analysis of variance followed by the Tukey test for comparison of means between groups (p ? 0. 05). Most of the observed changes in the erythrogram were significantly higher for the anticoagulant heparin and benzocaine group in comparison to the control group. However, the values obtained for the leukogram were significantly higher for all groups subjected to the Na2EDTA anticoagulant, suggesting that heparin may cause cell clumping. The results suggest that the anesthetics under investigation effectively minimizes the effects of stress caused by handling and invasive procedures, and that the anticoagulant heparin causes less hemolysis in comparison to Na2EDTA for Nile tilapia. Thus, the hematological variations attributed to different anesthetic protocols and/or different anticoagulants should be considered for the species Oreochromis niloticus. |
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Semina. Ciências Agrárias (Online) |
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Hematology of Nile tilapia (Oreochromis niloticus) subjected to anesthesia and anticoagulation protocolsHematologia de tilápia do Nilo (Oreochromis niloticus) submetidas a protocolos anestésicos e de anticoagulaçãoAnestheticsAnticoagulantsHematologyOreochromis niloticusOsmotic fragility.AnestésicosAnticoagulantesHematologiaFragilidade osmóticaOreochromis niloticus.Clinical hematology facilitates the diagnosis of disease and can act as a prognostic indicator of pathological conditions in fish. The aim of the present study was to evaluate hematological parameters of Nile tilapia (Oreochromis niloticus) subjected to different anesthetics and anticoagulants. Thirty apparently healthy fishes (average weight of 473 ± 35. 50 g and mean total length of 29. 33 ± 0. 37 cm), were selected from the local commercial fish farm in the Lages municipality (Santa Catarina, Brazil). The animals were randomly divided into three groups of 10. In two groups, anesthesia was induced with eugenol (70 mg·L- 1) (EG) and Benzocaine hydrochloride (100 mg·L-1) (BG), respectively. Anesthesia was not administered to fish of the third group (CG/control group). Blood samples were obtained by venipuncture of the caudal vessels and placed into microtubes containing sodium heparin or Na2EDTA for further analysis. The results were analyzed by Sigma Stat for Windows, the paired t-test for significant differences between anticoagulants of the same group, and analysis of variance followed by the Tukey test for comparison of means between groups (p ? 0. 05). Most of the observed changes in the erythrogram were significantly higher for the anticoagulant heparin and benzocaine group in comparison to the control group. However, the values obtained for the leukogram were significantly higher for all groups subjected to the Na2EDTA anticoagulant, suggesting that heparin may cause cell clumping. The results suggest that the anesthetics under investigation effectively minimizes the effects of stress caused by handling and invasive procedures, and that the anticoagulant heparin causes less hemolysis in comparison to Na2EDTA for Nile tilapia. Thus, the hematological variations attributed to different anesthetic protocols and/or different anticoagulants should be considered for the species Oreochromis niloticus. A hematologia clínica é uma ferramenta que auxilia na realização do diagnóstico de doenças e pode atuar como um indicador prognóstico das condições patológicas de peixes. O objetivo deste estudo foi avaliar os valores hematológicos de tilápias do Nilo (Oreochromis niloticus) submetidas a diferentes anestésicos e amostras obtidas com diferentes anticoagulantes. Foram utilizados trinta peixes, com peso médio de 473±35,50g e comprimento total médio de 29,33±0,37cm, adquiridos de piscicultura comercial localizada no município de Lages (SC). Os animais foram divididos aleatoriamente em três grupos (n=10) conforme a forma de indução: grupo eugenol (GE) na concentração de 70mg L-1, grupo cloridrato de benzocaína (GB) na concentração de 100mg L-1 e grupo controle (GC) sem o uso de anestésicos. As amostras de sangue foram obtidas por venopunção dos vasos caudais e acondicionadas em microtubos contendo heparina sódica ou Na2EDTA e posteriormente processadas. Os resultados foram avaliados pelo Sigma Stat, sendo utilizado o teste-t pareado para dados entre diferentes anticoagulantes pertencentes ao mesmo grupo e análise de variância, seguida de teste de Tukey para comparação das médias entre grupos (p?0,05). A maioria das alterações observadas no eritrograma foram superiores com o anticoagulante heparina e no grupo benzocaína comparados com o grupo controle. Porém no leucograma, os valores obtidos foram maiores com o anticoagulante Na2EDTA em todos os grupos, sugerindo que a heparina pode causar aglomeração celular. Baseado nos resultados encontrados conclui-se que os anestésicos testados minimizaram o efeito do estresse causado por manipulações e procedimentos invasivos quando comparado ao grupo controle e a heparina foi o anticoagulante que causou menor hemólise quando comparado com o Na2EDTA para esta espécie no teste de fragilidade osmótica eritrocitária. Assim, as variações hematológicas a partir de amostras obtidas com diferentes protocolos anestésicos e/ou diferentes anticoagulantes devem ser consideradas para a espécie Oreochromis niloticus. UEL2015-12-16info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionapplication/pdfhttps://ojs.uel.br/revistas/uel/index.php/semagrarias/article/view/1982910.5433/1679-0359.2015v36n6Supl2p4237Semina: Ciências Agrárias; Vol. 36 No. 6Supl2 (2015); 4237-4250Semina: Ciências Agrárias; v. 36 n. 6Supl2 (2015); 4237-42501679-03591676-546Xreponame:Semina. Ciências Agrárias (Online)instname:Universidade Estadual de Londrina (UEL)instacron:UELenghttps://ojs.uel.br/revistas/uel/index.php/semagrarias/article/view/19829/17593http://creativecommons.org/licenses/by-nc/4.0info:eu-repo/semantics/openAccessWeinert, Nadia CristineVolpato, JulietaCosta, ÁdsonAntunes, Rozyanne RosaOliveira, Aldo Camargo deMattoso, Cláudio Roberto ScabeloSaito, Mere Erika2022-12-02T14:52:17Zoai:ojs.pkp.sfu.ca:article/19829Revistahttp://www.uel.br/revistas/uel/index.php/semagrariasPUBhttps://ojs.uel.br/revistas/uel/index.php/semagrarias/oaisemina.agrarias@uel.br1679-03591676-546Xopendoar:2022-12-02T14:52:17Semina. Ciências Agrárias (Online) - Universidade Estadual de Londrina (UEL)false |
dc.title.none.fl_str_mv |
Hematology of Nile tilapia (Oreochromis niloticus) subjected to anesthesia and anticoagulation protocols Hematologia de tilápia do Nilo (Oreochromis niloticus) submetidas a protocolos anestésicos e de anticoagulação |
title |
Hematology of Nile tilapia (Oreochromis niloticus) subjected to anesthesia and anticoagulation protocols |
spellingShingle |
Hematology of Nile tilapia (Oreochromis niloticus) subjected to anesthesia and anticoagulation protocols Weinert, Nadia Cristine Anesthetics Anticoagulants Hematology Oreochromis niloticus Osmotic fragility. Anestésicos Anticoagulantes Hematologia Fragilidade osmótica Oreochromis niloticus. |
title_short |
Hematology of Nile tilapia (Oreochromis niloticus) subjected to anesthesia and anticoagulation protocols |
title_full |
Hematology of Nile tilapia (Oreochromis niloticus) subjected to anesthesia and anticoagulation protocols |
title_fullStr |
Hematology of Nile tilapia (Oreochromis niloticus) subjected to anesthesia and anticoagulation protocols |
title_full_unstemmed |
Hematology of Nile tilapia (Oreochromis niloticus) subjected to anesthesia and anticoagulation protocols |
title_sort |
Hematology of Nile tilapia (Oreochromis niloticus) subjected to anesthesia and anticoagulation protocols |
author |
Weinert, Nadia Cristine |
author_facet |
Weinert, Nadia Cristine Volpato, Julieta Costa, Ádson Antunes, Rozyanne Rosa Oliveira, Aldo Camargo de Mattoso, Cláudio Roberto Scabelo Saito, Mere Erika |
author_role |
author |
author2 |
Volpato, Julieta Costa, Ádson Antunes, Rozyanne Rosa Oliveira, Aldo Camargo de Mattoso, Cláudio Roberto Scabelo Saito, Mere Erika |
author2_role |
author author author author author author |
dc.contributor.author.fl_str_mv |
Weinert, Nadia Cristine Volpato, Julieta Costa, Ádson Antunes, Rozyanne Rosa Oliveira, Aldo Camargo de Mattoso, Cláudio Roberto Scabelo Saito, Mere Erika |
dc.subject.por.fl_str_mv |
Anesthetics Anticoagulants Hematology Oreochromis niloticus Osmotic fragility. Anestésicos Anticoagulantes Hematologia Fragilidade osmótica Oreochromis niloticus. |
topic |
Anesthetics Anticoagulants Hematology Oreochromis niloticus Osmotic fragility. Anestésicos Anticoagulantes Hematologia Fragilidade osmótica Oreochromis niloticus. |
description |
Clinical hematology facilitates the diagnosis of disease and can act as a prognostic indicator of pathological conditions in fish. The aim of the present study was to evaluate hematological parameters of Nile tilapia (Oreochromis niloticus) subjected to different anesthetics and anticoagulants. Thirty apparently healthy fishes (average weight of 473 ± 35. 50 g and mean total length of 29. 33 ± 0. 37 cm), were selected from the local commercial fish farm in the Lages municipality (Santa Catarina, Brazil). The animals were randomly divided into three groups of 10. In two groups, anesthesia was induced with eugenol (70 mg·L- 1) (EG) and Benzocaine hydrochloride (100 mg·L-1) (BG), respectively. Anesthesia was not administered to fish of the third group (CG/control group). Blood samples were obtained by venipuncture of the caudal vessels and placed into microtubes containing sodium heparin or Na2EDTA for further analysis. The results were analyzed by Sigma Stat for Windows, the paired t-test for significant differences between anticoagulants of the same group, and analysis of variance followed by the Tukey test for comparison of means between groups (p ? 0. 05). Most of the observed changes in the erythrogram were significantly higher for the anticoagulant heparin and benzocaine group in comparison to the control group. However, the values obtained for the leukogram were significantly higher for all groups subjected to the Na2EDTA anticoagulant, suggesting that heparin may cause cell clumping. The results suggest that the anesthetics under investigation effectively minimizes the effects of stress caused by handling and invasive procedures, and that the anticoagulant heparin causes less hemolysis in comparison to Na2EDTA for Nile tilapia. Thus, the hematological variations attributed to different anesthetic protocols and/or different anticoagulants should be considered for the species Oreochromis niloticus. |
publishDate |
2015 |
dc.date.none.fl_str_mv |
2015-12-16 |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
https://ojs.uel.br/revistas/uel/index.php/semagrarias/article/view/19829 10.5433/1679-0359.2015v36n6Supl2p4237 |
url |
https://ojs.uel.br/revistas/uel/index.php/semagrarias/article/view/19829 |
identifier_str_mv |
10.5433/1679-0359.2015v36n6Supl2p4237 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
https://ojs.uel.br/revistas/uel/index.php/semagrarias/article/view/19829/17593 |
dc.rights.driver.fl_str_mv |
http://creativecommons.org/licenses/by-nc/4.0 info:eu-repo/semantics/openAccess |
rights_invalid_str_mv |
http://creativecommons.org/licenses/by-nc/4.0 |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
application/pdf |
dc.publisher.none.fl_str_mv |
UEL |
publisher.none.fl_str_mv |
UEL |
dc.source.none.fl_str_mv |
Semina: Ciências Agrárias; Vol. 36 No. 6Supl2 (2015); 4237-4250 Semina: Ciências Agrárias; v. 36 n. 6Supl2 (2015); 4237-4250 1679-0359 1676-546X reponame:Semina. Ciências Agrárias (Online) instname:Universidade Estadual de Londrina (UEL) instacron:UEL |
instname_str |
Universidade Estadual de Londrina (UEL) |
instacron_str |
UEL |
institution |
UEL |
reponame_str |
Semina. Ciências Agrárias (Online) |
collection |
Semina. Ciências Agrárias (Online) |
repository.name.fl_str_mv |
Semina. Ciências Agrárias (Online) - Universidade Estadual de Londrina (UEL) |
repository.mail.fl_str_mv |
semina.agrarias@uel.br |
_version_ |
1799306072820809728 |