Influence of hydrolized casein on the Oncidium baueri (Orchidaceae) in vitro cultivation
Autor(a) principal: | |
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Data de Publicação: | 2007 |
Outros Autores: | , , , |
Tipo de documento: | Artigo |
Idioma: | por |
Título da fonte: | Semina. Ciências Agrárias (Online) |
DOI: | 10.5433/1679-0359.2007v28n2p207 |
Texto Completo: | https://ojs.uel.br/revistas/uel/index.php/semagrarias/article/view/3429 |
Resumo: | The nitrogen is essentially important for most in vitro cultivated species. It is regarded to the physiological and biochemical processes involved in growth control, differentiation and morphogenesis, and casein is one of the main nitrogen supplementation sources. The objective of this work was to evaluate the influence of different concentrations of hydrolized casein on the in vitro vegetative growth and rooting of Oncidium baueri (Orchidaceae). The treatments tested were: T1- 0.0 g.L-1; T2- 0.5 g.L-1; T3- 1.0 g. L-1; T4 –2.0 g.L-1; T5- 4.0 g.L-1 of hydrolized casein. The culture medium used was the Murashige and Skoog modified with half macronutrients concentration. Total fresh mass, aerial part height, biggest root length, roots number and lateral sprouts number were evaluated after six months from the beginning of the experiment. It was not detected any beneficial effects of casein on vegetative and root development of Oncidium baueri. The percentage of surviving plants was 100%, 95 % and 94%, respectively for T1, T2 and T3, and 80% of surviving plants for T4 and T5. |
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Semina. Ciências Agrárias (Online) |
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Influence of hydrolized casein on the Oncidium baueri (Orchidaceae) in vitro cultivationInfluência da caseína hidrolisada no cultivo in vitro de Oncidium baueri (Orchidaceae)Nitrogenorchidtissue cultureNitrogênioorquídeacultura de tecidosThe nitrogen is essentially important for most in vitro cultivated species. It is regarded to the physiological and biochemical processes involved in growth control, differentiation and morphogenesis, and casein is one of the main nitrogen supplementation sources. The objective of this work was to evaluate the influence of different concentrations of hydrolized casein on the in vitro vegetative growth and rooting of Oncidium baueri (Orchidaceae). The treatments tested were: T1- 0.0 g.L-1; T2- 0.5 g.L-1; T3- 1.0 g. L-1; T4 –2.0 g.L-1; T5- 4.0 g.L-1 of hydrolized casein. The culture medium used was the Murashige and Skoog modified with half macronutrients concentration. Total fresh mass, aerial part height, biggest root length, roots number and lateral sprouts number were evaluated after six months from the beginning of the experiment. It was not detected any beneficial effects of casein on vegetative and root development of Oncidium baueri. The percentage of surviving plants was 100%, 95 % and 94%, respectively for T1, T2 and T3, and 80% of surviving plants for T4 and T5. O nitrogênio possui grande importância para a maioria das espécies cultivadas in vitro. Está envolvido nos processos fisiológicos e bioquímicos associados ao controle de crescimento, diferenciação e morfogênese, sendo a caseína uma das principais fontes de suplementação do nitrogênio. O objetivo deste trabalho foi avaliar a influência de diferentes concentrações de caseína hidrolisada no crescimento vegetativo e enraizamento in vitro de Oncidium baueri (Orchidaceae). Os tratamentos testados foram: T1- 0,0 g.L-1; T2- 0,5 g.L-1 de caseína hidrolisada; T3- 1,0 g.L-1 de caseína hidrolisada; T4- 2,0 g.L-1 de caseína hidrolisada e T5- 4,0 g.L-1 de caseína hidrolisada. O meio de cultura utilizado foi o Murashige e Skoog (1962), modificado com metade da concentração de macronutrientes. Após seis meses do início do experimento foram avaliados: massa fresca total; altura da parte aérea; comprimento da maior raiz; número de raízes e número de brotações laterais. Não se observou efeito benéfico no desenvolvimento vegetativo e radicular de Oncidium baueri em meio de cultura suplementado com caseína hidrolisada. A porcentagem de sobrevivência foi 100%, 95% e 94%, respectivamente para T1, T2 e T3, e 80% para T4 e T5.UEL2007-10-30info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionapplication/pdfhttps://ojs.uel.br/revistas/uel/index.php/semagrarias/article/view/342910.5433/1679-0359.2007v28n2p207Semina: Ciências Agrárias; Vol. 28 No. 2 (2007); 207-212Semina: Ciências Agrárias; v. 28 n. 2 (2007); 207-2121679-03591676-546Xreponame:Semina. Ciências Agrárias (Online)instname:Universidade Estadual de Londrina (UEL)instacron:UELporhttps://ojs.uel.br/revistas/uel/index.php/semagrarias/article/view/3429/2788Vieira, José Geraldo ZaparolliYamakami, Jorge KaoroAguiar, Ricardo Sfeir deUnemoto, Lilian KeikoFaria, Ricardo Tadeu deinfo:eu-repo/semantics/openAccess2009-10-08T18:50:47Zoai:ojs.pkp.sfu.ca:article/3429Revistahttp://www.uel.br/revistas/uel/index.php/semagrariasPUBhttps://ojs.uel.br/revistas/uel/index.php/semagrarias/oaisemina.agrarias@uel.br1679-03591676-546Xopendoar:2009-10-08T18:50:47Semina. Ciências Agrárias (Online) - Universidade Estadual de Londrina (UEL)false |
dc.title.none.fl_str_mv |
Influence of hydrolized casein on the Oncidium baueri (Orchidaceae) in vitro cultivation Influência da caseína hidrolisada no cultivo in vitro de Oncidium baueri (Orchidaceae) |
title |
Influence of hydrolized casein on the Oncidium baueri (Orchidaceae) in vitro cultivation |
spellingShingle |
Influence of hydrolized casein on the Oncidium baueri (Orchidaceae) in vitro cultivation Influence of hydrolized casein on the Oncidium baueri (Orchidaceae) in vitro cultivation Vieira, José Geraldo Zaparolli Nitrogen orchid tissue culture Nitrogênio orquídea cultura de tecidos Vieira, José Geraldo Zaparolli Nitrogen orchid tissue culture Nitrogênio orquídea cultura de tecidos |
title_short |
Influence of hydrolized casein on the Oncidium baueri (Orchidaceae) in vitro cultivation |
title_full |
Influence of hydrolized casein on the Oncidium baueri (Orchidaceae) in vitro cultivation |
title_fullStr |
Influence of hydrolized casein on the Oncidium baueri (Orchidaceae) in vitro cultivation Influence of hydrolized casein on the Oncidium baueri (Orchidaceae) in vitro cultivation |
title_full_unstemmed |
Influence of hydrolized casein on the Oncidium baueri (Orchidaceae) in vitro cultivation Influence of hydrolized casein on the Oncidium baueri (Orchidaceae) in vitro cultivation |
title_sort |
Influence of hydrolized casein on the Oncidium baueri (Orchidaceae) in vitro cultivation |
author |
Vieira, José Geraldo Zaparolli |
author_facet |
Vieira, José Geraldo Zaparolli Vieira, José Geraldo Zaparolli Yamakami, Jorge Kaoro Aguiar, Ricardo Sfeir de Unemoto, Lilian Keiko Faria, Ricardo Tadeu de Yamakami, Jorge Kaoro Aguiar, Ricardo Sfeir de Unemoto, Lilian Keiko Faria, Ricardo Tadeu de |
author_role |
author |
author2 |
Yamakami, Jorge Kaoro Aguiar, Ricardo Sfeir de Unemoto, Lilian Keiko Faria, Ricardo Tadeu de |
author2_role |
author author author author |
dc.contributor.author.fl_str_mv |
Vieira, José Geraldo Zaparolli Yamakami, Jorge Kaoro Aguiar, Ricardo Sfeir de Unemoto, Lilian Keiko Faria, Ricardo Tadeu de |
dc.subject.por.fl_str_mv |
Nitrogen orchid tissue culture Nitrogênio orquídea cultura de tecidos |
topic |
Nitrogen orchid tissue culture Nitrogênio orquídea cultura de tecidos |
description |
The nitrogen is essentially important for most in vitro cultivated species. It is regarded to the physiological and biochemical processes involved in growth control, differentiation and morphogenesis, and casein is one of the main nitrogen supplementation sources. The objective of this work was to evaluate the influence of different concentrations of hydrolized casein on the in vitro vegetative growth and rooting of Oncidium baueri (Orchidaceae). The treatments tested were: T1- 0.0 g.L-1; T2- 0.5 g.L-1; T3- 1.0 g. L-1; T4 –2.0 g.L-1; T5- 4.0 g.L-1 of hydrolized casein. The culture medium used was the Murashige and Skoog modified with half macronutrients concentration. Total fresh mass, aerial part height, biggest root length, roots number and lateral sprouts number were evaluated after six months from the beginning of the experiment. It was not detected any beneficial effects of casein on vegetative and root development of Oncidium baueri. The percentage of surviving plants was 100%, 95 % and 94%, respectively for T1, T2 and T3, and 80% of surviving plants for T4 and T5. |
publishDate |
2007 |
dc.date.none.fl_str_mv |
2007-10-30 |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
https://ojs.uel.br/revistas/uel/index.php/semagrarias/article/view/3429 10.5433/1679-0359.2007v28n2p207 |
url |
https://ojs.uel.br/revistas/uel/index.php/semagrarias/article/view/3429 |
identifier_str_mv |
10.5433/1679-0359.2007v28n2p207 |
dc.language.iso.fl_str_mv |
por |
language |
por |
dc.relation.none.fl_str_mv |
https://ojs.uel.br/revistas/uel/index.php/semagrarias/article/view/3429/2788 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
application/pdf |
dc.publisher.none.fl_str_mv |
UEL |
publisher.none.fl_str_mv |
UEL |
dc.source.none.fl_str_mv |
Semina: Ciências Agrárias; Vol. 28 No. 2 (2007); 207-212 Semina: Ciências Agrárias; v. 28 n. 2 (2007); 207-212 1679-0359 1676-546X reponame:Semina. Ciências Agrárias (Online) instname:Universidade Estadual de Londrina (UEL) instacron:UEL |
instname_str |
Universidade Estadual de Londrina (UEL) |
instacron_str |
UEL |
institution |
UEL |
reponame_str |
Semina. Ciências Agrárias (Online) |
collection |
Semina. Ciências Agrárias (Online) |
repository.name.fl_str_mv |
Semina. Ciências Agrárias (Online) - Universidade Estadual de Londrina (UEL) |
repository.mail.fl_str_mv |
semina.agrarias@uel.br |
_version_ |
1822182790612910080 |
dc.identifier.doi.none.fl_str_mv |
10.5433/1679-0359.2007v28n2p207 |