Influence of hydrolized casein on the Oncidium baueri (Orchidaceae) in vitro cultivation

Detalhes bibliográficos
Autor(a) principal: Vieira, José Geraldo Zaparolli
Data de Publicação: 2007
Outros Autores: Yamakami, Jorge Kaoro, Aguiar, Ricardo Sfeir de, Unemoto, Lilian Keiko, Faria, Ricardo Tadeu de
Tipo de documento: Artigo
Idioma: por
Título da fonte: Semina. Ciências Agrárias (Online)
DOI: 10.5433/1679-0359.2007v28n2p207
Texto Completo: https://ojs.uel.br/revistas/uel/index.php/semagrarias/article/view/3429
Resumo: The nitrogen is essentially important for most in vitro cultivated species. It is regarded to the physiological and biochemical processes involved in growth control, differentiation and morphogenesis, and casein is one of the main nitrogen supplementation sources. The objective of this work was to evaluate the influence of different concentrations of hydrolized casein on the in vitro vegetative growth and rooting of Oncidium baueri (Orchidaceae). The treatments tested were: T1- 0.0 g.L-1; T2- 0.5 g.L-1; T3- 1.0 g. L-1; T4 –2.0 g.L-1; T5- 4.0 g.L-1 of hydrolized casein. The culture medium used was the Murashige and Skoog modified with half macronutrients concentration. Total fresh mass, aerial part height, biggest root length, roots number and lateral sprouts number were evaluated after six months from the beginning of the experiment. It was not detected any beneficial effects of casein on vegetative and root development of Oncidium baueri. The percentage of surviving plants was 100%, 95 % and 94%, respectively for T1, T2 and T3, and 80% of surviving plants for T4 and T5.
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spelling Influence of hydrolized casein on the Oncidium baueri (Orchidaceae) in vitro cultivationInfluência da caseína hidrolisada no cultivo in vitro de Oncidium baueri (Orchidaceae)Nitrogenorchidtissue cultureNitrogênioorquídeacultura de tecidosThe nitrogen is essentially important for most in vitro cultivated species. It is regarded to the physiological and biochemical processes involved in growth control, differentiation and morphogenesis, and casein is one of the main nitrogen supplementation sources. The objective of this work was to evaluate the influence of different concentrations of hydrolized casein on the in vitro vegetative growth and rooting of Oncidium baueri (Orchidaceae). The treatments tested were: T1- 0.0 g.L-1; T2- 0.5 g.L-1; T3- 1.0 g. L-1; T4 –2.0 g.L-1; T5- 4.0 g.L-1 of hydrolized casein. The culture medium used was the Murashige and Skoog modified with half macronutrients concentration. Total fresh mass, aerial part height, biggest root length, roots number and lateral sprouts number were evaluated after six months from the beginning of the experiment. It was not detected any beneficial effects of casein on vegetative and root development of Oncidium baueri. The percentage of surviving plants was 100%, 95 % and 94%, respectively for T1, T2 and T3, and 80% of surviving plants for T4 and T5. O nitrogênio possui grande importância para a maioria das espécies cultivadas in vitro. Está envolvido nos processos fisiológicos e bioquímicos associados ao controle de crescimento, diferenciação e morfogênese, sendo a caseína uma das principais fontes de suplementação do nitrogênio. O objetivo deste trabalho foi avaliar a influência de diferentes concentrações de caseína hidrolisada no crescimento vegetativo e enraizamento in vitro de Oncidium baueri (Orchidaceae). Os tratamentos testados foram: T1- 0,0 g.L-1; T2- 0,5 g.L-1 de caseína hidrolisada; T3- 1,0 g.L-1 de caseína hidrolisada; T4- 2,0 g.L-1 de caseína hidrolisada e T5- 4,0 g.L-1 de caseína hidrolisada. O meio de cultura utilizado foi o Murashige e Skoog (1962), modificado com metade da concentração de macronutrientes. Após seis meses do início do experimento foram avaliados: massa fresca total; altura da parte aérea; comprimento da maior raiz; número de raízes e número de brotações laterais. Não se observou efeito benéfico no desenvolvimento vegetativo e radicular de Oncidium baueri em meio de cultura suplementado com caseína hidrolisada. A porcentagem de sobrevivência foi 100%, 95% e 94%, respectivamente para T1, T2 e T3, e 80% para T4 e T5.UEL2007-10-30info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionapplication/pdfhttps://ojs.uel.br/revistas/uel/index.php/semagrarias/article/view/342910.5433/1679-0359.2007v28n2p207Semina: Ciências Agrárias; Vol. 28 No. 2 (2007); 207-212Semina: Ciências Agrárias; v. 28 n. 2 (2007); 207-2121679-03591676-546Xreponame:Semina. Ciências Agrárias (Online)instname:Universidade Estadual de Londrina (UEL)instacron:UELporhttps://ojs.uel.br/revistas/uel/index.php/semagrarias/article/view/3429/2788Vieira, José Geraldo ZaparolliYamakami, Jorge KaoroAguiar, Ricardo Sfeir deUnemoto, Lilian KeikoFaria, Ricardo Tadeu deinfo:eu-repo/semantics/openAccess2009-10-08T18:50:47Zoai:ojs.pkp.sfu.ca:article/3429Revistahttp://www.uel.br/revistas/uel/index.php/semagrariasPUBhttps://ojs.uel.br/revistas/uel/index.php/semagrarias/oaisemina.agrarias@uel.br1679-03591676-546Xopendoar:2009-10-08T18:50:47Semina. Ciências Agrárias (Online) - Universidade Estadual de Londrina (UEL)false
dc.title.none.fl_str_mv Influence of hydrolized casein on the Oncidium baueri (Orchidaceae) in vitro cultivation
Influência da caseína hidrolisada no cultivo in vitro de Oncidium baueri (Orchidaceae)
title Influence of hydrolized casein on the Oncidium baueri (Orchidaceae) in vitro cultivation
spellingShingle Influence of hydrolized casein on the Oncidium baueri (Orchidaceae) in vitro cultivation
Influence of hydrolized casein on the Oncidium baueri (Orchidaceae) in vitro cultivation
Vieira, José Geraldo Zaparolli
Nitrogen
orchid
tissue culture
Nitrogênio
orquídea
cultura de tecidos
Vieira, José Geraldo Zaparolli
Nitrogen
orchid
tissue culture
Nitrogênio
orquídea
cultura de tecidos
title_short Influence of hydrolized casein on the Oncidium baueri (Orchidaceae) in vitro cultivation
title_full Influence of hydrolized casein on the Oncidium baueri (Orchidaceae) in vitro cultivation
title_fullStr Influence of hydrolized casein on the Oncidium baueri (Orchidaceae) in vitro cultivation
Influence of hydrolized casein on the Oncidium baueri (Orchidaceae) in vitro cultivation
title_full_unstemmed Influence of hydrolized casein on the Oncidium baueri (Orchidaceae) in vitro cultivation
Influence of hydrolized casein on the Oncidium baueri (Orchidaceae) in vitro cultivation
title_sort Influence of hydrolized casein on the Oncidium baueri (Orchidaceae) in vitro cultivation
author Vieira, José Geraldo Zaparolli
author_facet Vieira, José Geraldo Zaparolli
Vieira, José Geraldo Zaparolli
Yamakami, Jorge Kaoro
Aguiar, Ricardo Sfeir de
Unemoto, Lilian Keiko
Faria, Ricardo Tadeu de
Yamakami, Jorge Kaoro
Aguiar, Ricardo Sfeir de
Unemoto, Lilian Keiko
Faria, Ricardo Tadeu de
author_role author
author2 Yamakami, Jorge Kaoro
Aguiar, Ricardo Sfeir de
Unemoto, Lilian Keiko
Faria, Ricardo Tadeu de
author2_role author
author
author
author
dc.contributor.author.fl_str_mv Vieira, José Geraldo Zaparolli
Yamakami, Jorge Kaoro
Aguiar, Ricardo Sfeir de
Unemoto, Lilian Keiko
Faria, Ricardo Tadeu de
dc.subject.por.fl_str_mv Nitrogen
orchid
tissue culture
Nitrogênio
orquídea
cultura de tecidos
topic Nitrogen
orchid
tissue culture
Nitrogênio
orquídea
cultura de tecidos
description The nitrogen is essentially important for most in vitro cultivated species. It is regarded to the physiological and biochemical processes involved in growth control, differentiation and morphogenesis, and casein is one of the main nitrogen supplementation sources. The objective of this work was to evaluate the influence of different concentrations of hydrolized casein on the in vitro vegetative growth and rooting of Oncidium baueri (Orchidaceae). The treatments tested were: T1- 0.0 g.L-1; T2- 0.5 g.L-1; T3- 1.0 g. L-1; T4 –2.0 g.L-1; T5- 4.0 g.L-1 of hydrolized casein. The culture medium used was the Murashige and Skoog modified with half macronutrients concentration. Total fresh mass, aerial part height, biggest root length, roots number and lateral sprouts number were evaluated after six months from the beginning of the experiment. It was not detected any beneficial effects of casein on vegetative and root development of Oncidium baueri. The percentage of surviving plants was 100%, 95 % and 94%, respectively for T1, T2 and T3, and 80% of surviving plants for T4 and T5.
publishDate 2007
dc.date.none.fl_str_mv 2007-10-30
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv https://ojs.uel.br/revistas/uel/index.php/semagrarias/article/view/3429
10.5433/1679-0359.2007v28n2p207
url https://ojs.uel.br/revistas/uel/index.php/semagrarias/article/view/3429
identifier_str_mv 10.5433/1679-0359.2007v28n2p207
dc.language.iso.fl_str_mv por
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dc.relation.none.fl_str_mv https://ojs.uel.br/revistas/uel/index.php/semagrarias/article/view/3429/2788
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
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dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv UEL
publisher.none.fl_str_mv UEL
dc.source.none.fl_str_mv Semina: Ciências Agrárias; Vol. 28 No. 2 (2007); 207-212
Semina: Ciências Agrárias; v. 28 n. 2 (2007); 207-212
1679-0359
1676-546X
reponame:Semina. Ciências Agrárias (Online)
instname:Universidade Estadual de Londrina (UEL)
instacron:UEL
instname_str Universidade Estadual de Londrina (UEL)
instacron_str UEL
institution UEL
reponame_str Semina. Ciências Agrárias (Online)
collection Semina. Ciências Agrárias (Online)
repository.name.fl_str_mv Semina. Ciências Agrárias (Online) - Universidade Estadual de Londrina (UEL)
repository.mail.fl_str_mv semina.agrarias@uel.br
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dc.identifier.doi.none.fl_str_mv 10.5433/1679-0359.2007v28n2p207