Epidemiological characterization and risk factors associated with lentiviral infection of small ruminants at animal fairs in the semiarid Sertão region of Pernambuco, Brazilian semiarid

Detalhes bibliográficos
Autor(a) principal: Alves, José Romero Alexandre
Data de Publicação: 2017
Outros Autores: Limeira, Clécio Henrique, Lima, Geilson Manoel de Souza, Pinheiro, Raymundo Rizaldo, Alves, Francisco Selmo Fernandes, Santos, Vanderlan Warlington Souza dos, Azevedo, Sérgio Santos de, Alves, Clebert José
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Semina. Ciências Agrárias (Online)
DOI: 10.5433/1679-0359.2017v38n4p1875
Texto Completo: https://ojs.uel.br/revistas/uel/index.php/semagrarias/article/view/24506
Resumo: Animal agglomerations at commercial events such as trade fairs represent an important facilitator in infectious disease transmission. Thus, it is crucial to understand the epidemiology of infectious diseases in small ruminants. The objective of this study was to examine lentiviral presence in goats and sheep traded at the animal fair of Tabira city (Sertão region of Pernambuco) and identify possible risk factors associated with infection. We collected serum samples from 233 crossbred goats and 119 crossbred sheep, belonging to 12 breeders, at the Tabira livestock fair from November 2014 to June 2015. An epidemiological questionnaire was used to analyze the risk factors. Agar gel immunodiffusion (AGID) and western blotting (WB) were performed to diagnose small ruminant lentivirus (SRLV) infection. In the AGID test, we used maedi-visna virus (MVV) antigens for sheep and caprine arthritis/encephalitis virus (strain Cork) (CAEV-Co) antigen for goats. The WB analysis used CAEV-Co antigen for both species. Variables from the questionnaire were analyzed with univariate and multivariate statistics. One seropositive goat but no sheep was identified via AGID. According to the WB results, 15/233 goats (6.44%; CI95% = 3.94 - 10.35%) and 8/119 sheep (6.72%; CI95% = 3.45 - 12.71%) were seropositive, totaling 23/352 reactive animals (6.53%; CI95% = 4.39 - 9.61%) from 12 herds. Annual vermifugation of the animals was a significant risk factor (odds ratio = 5.9; CI95% = 1.7-19.8; p = 0.04) for disease in goats, but no variables associated with infection risks were identified in sheep. We concluded that SRLV was present in goats and sheep at the animal fair. Western blots were more sensitive than AGID for SRLV diagnosis. Practices aiming to improve sanitary management may reduce the risk of infection in goats. All studied herds included animals identified as seropositive for SRLV. Therefore, we recommend adopting measures that increase disease diagnosis while intensifying traffic control and surveillance of animal agglomerations.
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spelling Epidemiological characterization and risk factors associated with lentiviral infection of small ruminants at animal fairs in the semiarid Sertão region of Pernambuco, Brazilian semiaridCaracterização epidemiológica e fatores de risco associados às lentiviroses em pequenos ruminantes comercializados em feira de animais no Sertão de Pernambuco, semiárido brasileiroAgar gel immunodiffusionAgglomerationsLentivirusSmall RuminantsWestern Blotting.AglomeraçõesImunodifusão em ágar-gelLentivirusPequenos RuminantesWestern Blotting.Animal agglomerations at commercial events such as trade fairs represent an important facilitator in infectious disease transmission. Thus, it is crucial to understand the epidemiology of infectious diseases in small ruminants. The objective of this study was to examine lentiviral presence in goats and sheep traded at the animal fair of Tabira city (Sertão region of Pernambuco) and identify possible risk factors associated with infection. We collected serum samples from 233 crossbred goats and 119 crossbred sheep, belonging to 12 breeders, at the Tabira livestock fair from November 2014 to June 2015. An epidemiological questionnaire was used to analyze the risk factors. Agar gel immunodiffusion (AGID) and western blotting (WB) were performed to diagnose small ruminant lentivirus (SRLV) infection. In the AGID test, we used maedi-visna virus (MVV) antigens for sheep and caprine arthritis/encephalitis virus (strain Cork) (CAEV-Co) antigen for goats. The WB analysis used CAEV-Co antigen for both species. Variables from the questionnaire were analyzed with univariate and multivariate statistics. One seropositive goat but no sheep was identified via AGID. According to the WB results, 15/233 goats (6.44%; CI95% = 3.94 - 10.35%) and 8/119 sheep (6.72%; CI95% = 3.45 - 12.71%) were seropositive, totaling 23/352 reactive animals (6.53%; CI95% = 4.39 - 9.61%) from 12 herds. Annual vermifugation of the animals was a significant risk factor (odds ratio = 5.9; CI95% = 1.7-19.8; p = 0.04) for disease in goats, but no variables associated with infection risks were identified in sheep. We concluded that SRLV was present in goats and sheep at the animal fair. Western blots were more sensitive than AGID for SRLV diagnosis. Practices aiming to improve sanitary management may reduce the risk of infection in goats. All studied herds included animals identified as seropositive for SRLV. Therefore, we recommend adopting measures that increase disease diagnosis while intensifying traffic control and surveillance of animal agglomerations.Aglomerações de animais em eventos comerciais do tipo feiras de negócios representam um importante elo na transmissão de doenças infecciosas, resultando na importância do conhecimento acerca da epidemiologia das doenças de pequenos ruminantes. Desta forma, o objetivo desse estudo foi determinar a presença das lentiviroses em caprinos e ovinos comercializados na feira de animais da cidade de Tabira, Sertão de Pernambuco, bem como a identificação de possíveis fatores de risco associados à enfermidade. Foram coletadas amostras de soro de 233 caprinos e 119 ovinos, sem raça definida, pertencentes a doze proprietários, na feira de animais vivos do município no período de novembro de 2014 a junho de 2015. Foi aplicado questionário epidemiológico para análise dos fatores de risco. O diagnóstico da infecção por Lentivirus de Pequenos Ruminantes (LVPR) nas espécies foi realizado pela Imunodifusão em ágar-gel (IDGA) e Western Blotting (WB). Na IDGA foram utilizados antígenos de Vírus Maedi-visna (MVV) para ovinos e de vírus da Artrite-Encefalite Caprina (CAEV), cepa CAEV- Cork para caprinos, e no WB foi utilizado a cepa CAEV- Cork como antígeno para ambas espécies. As variáveis obtidas no questionário foram submetidas a análises estatísticas univariada e multivariada. Pela técnica de IDGA foi possível identificar um caprino soropositivo. Não foi detectada positividade nos ovinos. No WB 15/233 (6,44%; IC95% = 3,94 - 10,35%) caprinos e 8/119 (6,72%; IC95% = 3,45 - 12,71%) ovinos foram soropositivos, totalizando 23/352 (6,53%; IC95% = 4,39 - 9,61%) animais reagentes. Os doze rebanhos estudados apresentaram animais soropositivos. Vermifugar os animais anualmente (odds ratio = 5,9; IC 95% = 1,7 - 19,8; p = 0,04) foi identificado como fator de risco para enfermidade em caprinos. Não foram identificadas variáveis associadas ao risco de infecção em ovinos. Concluiu-se que os LVPR estão presentes em caprinos e ovinos comercializados na feira de animais. A técnica de WB apresentou maior sensibilidade que a IDGA para diagnóstico dos LVPR. Melhorias nas práticas de manejo sanitário podem reduzir o risco de infecção nos caprinos. Todos os rebanhos estudados apresentaram animais soropositivos para lentiviroses de pequenos ruminantes (SRLV), sendo recomendado adotar medidas que possibilitem aumentar o diagnóstico da doença e intensificar o controle de trânsito e fiscalização das aglomerações animais.UEL2017-08-04info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionPesquisa Empírica de Campoapplication/pdfhttps://ojs.uel.br/revistas/uel/index.php/semagrarias/article/view/2450610.5433/1679-0359.2017v38n4p1875Semina: Ciências Agrárias; Vol. 38 No. 4 (2017); 1875-1886Semina: Ciências Agrárias; v. 38 n. 4 (2017); 1875-18861679-03591676-546Xreponame:Semina. Ciências Agrárias (Online)instname:Universidade Estadual de Londrina (UEL)instacron:UELenghttps://ojs.uel.br/revistas/uel/index.php/semagrarias/article/view/24506/21365Copyright (c) 2017 Semina: Ciências Agráriashttp://creativecommons.org/licenses/by-nc/4.0info:eu-repo/semantics/openAccessAlves, José Romero AlexandreLimeira, Clécio HenriqueLima, Geilson Manoel de SouzaPinheiro, Raymundo RizaldoAlves, Francisco Selmo FernandesSantos, Vanderlan Warlington Souza dosAzevedo, Sérgio Santos deAlves, Clebert José2022-10-24T12:23:48Zoai:ojs.pkp.sfu.ca:article/24506Revistahttp://www.uel.br/revistas/uel/index.php/semagrariasPUBhttps://ojs.uel.br/revistas/uel/index.php/semagrarias/oaisemina.agrarias@uel.br1679-03591676-546Xopendoar:2022-10-24T12:23:48Semina. Ciências Agrárias (Online) - Universidade Estadual de Londrina (UEL)false
dc.title.none.fl_str_mv Epidemiological characterization and risk factors associated with lentiviral infection of small ruminants at animal fairs in the semiarid Sertão region of Pernambuco, Brazilian semiarid
Caracterização epidemiológica e fatores de risco associados às lentiviroses em pequenos ruminantes comercializados em feira de animais no Sertão de Pernambuco, semiárido brasileiro
title Epidemiological characterization and risk factors associated with lentiviral infection of small ruminants at animal fairs in the semiarid Sertão region of Pernambuco, Brazilian semiarid
spellingShingle Epidemiological characterization and risk factors associated with lentiviral infection of small ruminants at animal fairs in the semiarid Sertão region of Pernambuco, Brazilian semiarid
Epidemiological characterization and risk factors associated with lentiviral infection of small ruminants at animal fairs in the semiarid Sertão region of Pernambuco, Brazilian semiarid
Alves, José Romero Alexandre
Agar gel immunodiffusion
Agglomerations
Lentivirus
Small Ruminants
Western Blotting.
Aglomerações
Imunodifusão em ágar-gel
Lentivirus
Pequenos Ruminantes
Western Blotting.
Alves, José Romero Alexandre
Agar gel immunodiffusion
Agglomerations
Lentivirus
Small Ruminants
Western Blotting.
Aglomerações
Imunodifusão em ágar-gel
Lentivirus
Pequenos Ruminantes
Western Blotting.
title_short Epidemiological characterization and risk factors associated with lentiviral infection of small ruminants at animal fairs in the semiarid Sertão region of Pernambuco, Brazilian semiarid
title_full Epidemiological characterization and risk factors associated with lentiviral infection of small ruminants at animal fairs in the semiarid Sertão region of Pernambuco, Brazilian semiarid
title_fullStr Epidemiological characterization and risk factors associated with lentiviral infection of small ruminants at animal fairs in the semiarid Sertão region of Pernambuco, Brazilian semiarid
Epidemiological characterization and risk factors associated with lentiviral infection of small ruminants at animal fairs in the semiarid Sertão region of Pernambuco, Brazilian semiarid
title_full_unstemmed Epidemiological characterization and risk factors associated with lentiviral infection of small ruminants at animal fairs in the semiarid Sertão region of Pernambuco, Brazilian semiarid
Epidemiological characterization and risk factors associated with lentiviral infection of small ruminants at animal fairs in the semiarid Sertão region of Pernambuco, Brazilian semiarid
title_sort Epidemiological characterization and risk factors associated with lentiviral infection of small ruminants at animal fairs in the semiarid Sertão region of Pernambuco, Brazilian semiarid
author Alves, José Romero Alexandre
author_facet Alves, José Romero Alexandre
Alves, José Romero Alexandre
Limeira, Clécio Henrique
Lima, Geilson Manoel de Souza
Pinheiro, Raymundo Rizaldo
Alves, Francisco Selmo Fernandes
Santos, Vanderlan Warlington Souza dos
Azevedo, Sérgio Santos de
Alves, Clebert José
Limeira, Clécio Henrique
Lima, Geilson Manoel de Souza
Pinheiro, Raymundo Rizaldo
Alves, Francisco Selmo Fernandes
Santos, Vanderlan Warlington Souza dos
Azevedo, Sérgio Santos de
Alves, Clebert José
author_role author
author2 Limeira, Clécio Henrique
Lima, Geilson Manoel de Souza
Pinheiro, Raymundo Rizaldo
Alves, Francisco Selmo Fernandes
Santos, Vanderlan Warlington Souza dos
Azevedo, Sérgio Santos de
Alves, Clebert José
author2_role author
author
author
author
author
author
author
dc.contributor.author.fl_str_mv Alves, José Romero Alexandre
Limeira, Clécio Henrique
Lima, Geilson Manoel de Souza
Pinheiro, Raymundo Rizaldo
Alves, Francisco Selmo Fernandes
Santos, Vanderlan Warlington Souza dos
Azevedo, Sérgio Santos de
Alves, Clebert José
dc.subject.por.fl_str_mv Agar gel immunodiffusion
Agglomerations
Lentivirus
Small Ruminants
Western Blotting.
Aglomerações
Imunodifusão em ágar-gel
Lentivirus
Pequenos Ruminantes
Western Blotting.
topic Agar gel immunodiffusion
Agglomerations
Lentivirus
Small Ruminants
Western Blotting.
Aglomerações
Imunodifusão em ágar-gel
Lentivirus
Pequenos Ruminantes
Western Blotting.
description Animal agglomerations at commercial events such as trade fairs represent an important facilitator in infectious disease transmission. Thus, it is crucial to understand the epidemiology of infectious diseases in small ruminants. The objective of this study was to examine lentiviral presence in goats and sheep traded at the animal fair of Tabira city (Sertão region of Pernambuco) and identify possible risk factors associated with infection. We collected serum samples from 233 crossbred goats and 119 crossbred sheep, belonging to 12 breeders, at the Tabira livestock fair from November 2014 to June 2015. An epidemiological questionnaire was used to analyze the risk factors. Agar gel immunodiffusion (AGID) and western blotting (WB) were performed to diagnose small ruminant lentivirus (SRLV) infection. In the AGID test, we used maedi-visna virus (MVV) antigens for sheep and caprine arthritis/encephalitis virus (strain Cork) (CAEV-Co) antigen for goats. The WB analysis used CAEV-Co antigen for both species. Variables from the questionnaire were analyzed with univariate and multivariate statistics. One seropositive goat but no sheep was identified via AGID. According to the WB results, 15/233 goats (6.44%; CI95% = 3.94 - 10.35%) and 8/119 sheep (6.72%; CI95% = 3.45 - 12.71%) were seropositive, totaling 23/352 reactive animals (6.53%; CI95% = 4.39 - 9.61%) from 12 herds. Annual vermifugation of the animals was a significant risk factor (odds ratio = 5.9; CI95% = 1.7-19.8; p = 0.04) for disease in goats, but no variables associated with infection risks were identified in sheep. We concluded that SRLV was present in goats and sheep at the animal fair. Western blots were more sensitive than AGID for SRLV diagnosis. Practices aiming to improve sanitary management may reduce the risk of infection in goats. All studied herds included animals identified as seropositive for SRLV. Therefore, we recommend adopting measures that increase disease diagnosis while intensifying traffic control and surveillance of animal agglomerations.
publishDate 2017
dc.date.none.fl_str_mv 2017-08-04
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
Pesquisa Empírica de Campo
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv https://ojs.uel.br/revistas/uel/index.php/semagrarias/article/view/24506
10.5433/1679-0359.2017v38n4p1875
url https://ojs.uel.br/revistas/uel/index.php/semagrarias/article/view/24506
identifier_str_mv 10.5433/1679-0359.2017v38n4p1875
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv https://ojs.uel.br/revistas/uel/index.php/semagrarias/article/view/24506/21365
dc.rights.driver.fl_str_mv Copyright (c) 2017 Semina: Ciências Agrárias
http://creativecommons.org/licenses/by-nc/4.0
info:eu-repo/semantics/openAccess
rights_invalid_str_mv Copyright (c) 2017 Semina: Ciências Agrárias
http://creativecommons.org/licenses/by-nc/4.0
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv UEL
publisher.none.fl_str_mv UEL
dc.source.none.fl_str_mv Semina: Ciências Agrárias; Vol. 38 No. 4 (2017); 1875-1886
Semina: Ciências Agrárias; v. 38 n. 4 (2017); 1875-1886
1679-0359
1676-546X
reponame:Semina. Ciências Agrárias (Online)
instname:Universidade Estadual de Londrina (UEL)
instacron:UEL
instname_str Universidade Estadual de Londrina (UEL)
instacron_str UEL
institution UEL
reponame_str Semina. Ciências Agrárias (Online)
collection Semina. Ciências Agrárias (Online)
repository.name.fl_str_mv Semina. Ciências Agrárias (Online) - Universidade Estadual de Londrina (UEL)
repository.mail.fl_str_mv semina.agrarias@uel.br
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dc.identifier.doi.none.fl_str_mv 10.5433/1679-0359.2017v38n4p1875