Extração de neolignanas de Piper regnellii usando fluido pressurizado

• Autor(a) principal: Lemos, Caroline Ortega Terra
• Data de Publicação: 2011
• Tipo de documento: Dissertação
• Idioma: por
• Título da fonte: Repositório Institucional da Universidade Estadual de Maringá (RI-UEM)
• Texto Completo: http://repositorio.uem.br:8080/jspui/handle/1/3763
• topic: Piper regnellii (Pariparoba); Neolignanas; Modelagem matemática; Atividade biológica; Extração supercrítica; Brasil.; Piper regnellii; Neolignans; Supercritical extraction; Biological activity; Mathematical modeling; Brazil.; Engenharias; Engenharia Química

Piper regnellii var.pallescens is popularly known as pariparoba caapeba and capeba.In folk medicine, its roots are used in the treatment of obstructions of the liver and spleen, wound healing, it reduces swelling and skin irritation.It also gives broad spectrum of antimicrobial activity.The application of supercritical technology for the extraction of bioactive compounds of this kind can be attractive due to low temperature operation and high purity of the extracts.In this context, the objective of this study was to evaluate the biological quality of supercritical extracts of Piper regnellii obtained under variation of pressure and temperature parameters and compare them with the extracts obtained by conventional method.For this task extracts from the leaves and stems of pariparoba were obtained in various ways to extract and subsequently characterized according to their composition and neolignans conocarpan and eupomatenoid-5.The supercritical extraction occurred at temperatures of 40 and 60 °C and at pressures ranging from 109.2 to 250 bar.Tests of antifungal, antimicrobial, antioxidant and cytotoxic activity were performed.Despite the low yield in mass of extract obtained in supercritical extraction, they have higher levels of neolignans and conocarpan eupomatenoid-5.Due to the high quality of these extracts, the biological activities tested proved best in the soxhlet extraction method.The extracts obtained by supercritical extraction showed MICs between 1.95 and 7.81 μg/mL against S.aureus and MICs from 0.98 to 7.81 μg/mL against MRSA.In tests with different species of the genus Candida, these same extracts showed MIC ranging from 7.81 to 250 μg/mL.In the analysis of cytotoxicity extracts were more toxic to the pathogens than for animal cell tested.In addition, the test material showed high antioxidant exhibiting EC50 values between 41.23 and 85.41 μg/mL. To represent the kinetics of extraction in a fixed-bed column a second-order mathematical model was employed and provedsatisfactory, based on the comparison between the experimental and theoretical results.Thus, the supercritical extraction is presented as the best method for obtaining bioactive compounds promising for the development of drugs based on natural products.