Transport with different benzocaine concentrations and its consequences on hematological parameters and gill parasite population of matrinxã Brycon cephalus (Günther, 1869) (Osteichthyes, Characidae)
Autor(a) principal: | |
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Data de Publicação: | 2008 |
Outros Autores: | , |
Tipo de documento: | Artigo |
Idioma: | por |
Título da fonte: | Acta Scientiarum Biological Sciences |
Texto Completo: | http://www.periodicos.uem.br/ojs/index.php/ActaSciBiolSci/article/view/2359 |
Resumo: | The objective of this study was to evaluate the transport stressing effects and the different benzocaine concentrations on hematological parameters and parasite population of matrinxã Brycon cephalus (Osteichthyes, Characidae). Thirty fish (1.0 kg mean weight) were hauled in four 200-L plastic tanks for four hours, at different benzocaine concentrations (B0 = 0.0 g/L, B1 = 5.0 g/L, B2 = 10.0 g/L and B3 = 20 g/L). Before loading, blood and gills of 5 fish were collected. Other three samplings were performed after transport, and 24 and 96 hours later (recovery period). Blood cortisol, glucose, hematocrit, white cell differential count and the number of the gill parasite Piscinoodinium sp. were determined. Plasma cortisol increased in all treatments after transport, returning to initial levels 24 hours later. Blood glucose was also elevated after transport in all treatments, but only B0 and B1 fish recovered initial levels in 24 hours. B2 and B3 fish did not recover the initial levels until the end of the experimental period. After transport, the lymphocytes relative percentage decreased and the neuthrophils relative percentage increased, in all treatments. Ninety-six hours later, only B0 and B1 fish had recovered the initial levels of these parameters. The number of Piscinoodinium sp. on the gills of B3 fish was higher than in the other treatments 96 hours after transportation. The use of benzocaine did not mitigate stress responses and some negative effects were observed in fish transported with the highest concentrations. |
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Transport with different benzocaine concentrations and its consequences on hematological parameters and gill parasite population of matrinxã Brycon cephalus (Günther, 1869) (Osteichthyes, Characidae)Estresse devido ao transporte e à ação da benzocaína em parâmetros hematológicos e população de parasitas em matrinxã, Brycon cephalus (Günther, 1869) (Osteichthyes, Characidae)transporteestressebenzocaínamatrinxãBrycon cephalus2.00.00.00-6 Ciências BiológicasThe objective of this study was to evaluate the transport stressing effects and the different benzocaine concentrations on hematological parameters and parasite population of matrinxã Brycon cephalus (Osteichthyes, Characidae). Thirty fish (1.0 kg mean weight) were hauled in four 200-L plastic tanks for four hours, at different benzocaine concentrations (B0 = 0.0 g/L, B1 = 5.0 g/L, B2 = 10.0 g/L and B3 = 20 g/L). Before loading, blood and gills of 5 fish were collected. Other three samplings were performed after transport, and 24 and 96 hours later (recovery period). Blood cortisol, glucose, hematocrit, white cell differential count and the number of the gill parasite Piscinoodinium sp. were determined. Plasma cortisol increased in all treatments after transport, returning to initial levels 24 hours later. Blood glucose was also elevated after transport in all treatments, but only B0 and B1 fish recovered initial levels in 24 hours. B2 and B3 fish did not recover the initial levels until the end of the experimental period. After transport, the lymphocytes relative percentage decreased and the neuthrophils relative percentage increased, in all treatments. Ninety-six hours later, only B0 and B1 fish had recovered the initial levels of these parameters. The number of Piscinoodinium sp. on the gills of B3 fish was higher than in the other treatments 96 hours after transportation. The use of benzocaine did not mitigate stress responses and some negative effects were observed in fish transported with the highest concentrations.O objetivo deste trabalho foi avaliar os efeitos do transporte, sob diferentes concentrações de benzocaína, em parâmetros hematológicos e na população de parasitas de brânquias do matrinxã Brycon cephalus (Osteichthyes, Characidae). Trinta peixes (peso médio 1,0 kg) foram transportados em tambores plásticos de 200 L por quatro horas. Cada tambor foi preparado com uma concentração de benzocaína (B0 = 0 mg/L; B1 = 5 mg/L; B2 = 10 mg/L e B3 = 20 mg/L). Anteriormente ao carregamento dos tambores, cinco peixes foram amostrados para determinar a condição inicial. Outras três amostragens foram feitas posteriormente: na chegada, 24 e 96 horas após o transporte. Os peixes transportados sob efeito da benzocaína apresentaram menor resposta de fuga durante a captura comparado aos peixes do B0, sendo que os peixes do B3 mostraram dificuldade de manter o equilíbrio durante a viagem. Após o transporte, registraram-se os níveis mais elevados de cortisol plasmático, em todos os tratamentos, com retorno aos níveis iniciais após 24 horas. A glicemia elevou-se na chegada, em todos os tratamentos, e após 24 horas apenas os peixes transportados nas duas concentrações mais altas ainda não haviam recuperado os valores iniciais. Na chegada, a porcentagem de linfócitos decresceu, permanecendo neste patamar após 24 horas, sendo que os peixes do B2 e B3 não retornaram à condição inicial até o final do período experimental. Foi observado aumento da porcentagem de neutrófilos, desde a chegada até 24 horas após o transporte, em todos os tratamentos. Os peixes do B2 e B3 mantiveram elevadas as porcentagens de neutrófilos até 96 horas após o transporte. Na última coleta, constatou-se que o número do parasita branquial Piscinoodinium sp. havia aumentado nos peixes do B3. Foi possível observar que o uso da benzocaína contribuiu com a elevação da glicemia e dos níveis plasmáticos do cortisol após o transporte, sendo registrados efeitos negativos das duas concentrações mais altas em vários parâmetros hematológicos e no número de Piscinoodinium sp. aderido às brânquias. Concluiu-se, portanto que o uso da benzocaína não reduziu o estresse causado pelo transporte em matrinxãs, atuando inclusive como agente estressor adicional.Universidade Estadual De Maringá2008-05-13info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionapplication/pdfhttp://www.periodicos.uem.br/ojs/index.php/ActaSciBiolSci/article/view/235910.4025/actascibiolsci.v24i0.2359Acta Scientiarum. Biological Sciences; Vol 24 (2002); 555-560Acta Scientiarum. Biological Sciences; v. 24 (2002); 555-5601807-863X1679-9283reponame:Acta Scientiarum Biological Sciencesinstname:Universidade Estadual de Maringá (UEM)instacron:UEMporhttp://www.periodicos.uem.br/ojs/index.php/ActaSciBiolSci/article/view/2359/2301Carneiro, Paulo César FalangheUrbinati, Elisabeth CriscuoloMartins, Maurício Laterçainfo:eu-repo/semantics/openAccess2022-11-23T17:33:24Zoai:periodicos.uem.br/ojs:article/2359Revistahttp://www.periodicos.uem.br/ojs/index.php/ActaSciBiolSciPUBhttp://www.periodicos.uem.br/ojs/index.php/ActaSciBiolSci/oai||actabiol@uem.br1807-863X1679-9283opendoar:2022-11-23T17:33:24Acta Scientiarum Biological Sciences - Universidade Estadual de Maringá (UEM)false |
dc.title.none.fl_str_mv |
Transport with different benzocaine concentrations and its consequences on hematological parameters and gill parasite population of matrinxã Brycon cephalus (Günther, 1869) (Osteichthyes, Characidae) Estresse devido ao transporte e à ação da benzocaína em parâmetros hematológicos e população de parasitas em matrinxã, Brycon cephalus (Günther, 1869) (Osteichthyes, Characidae) |
title |
Transport with different benzocaine concentrations and its consequences on hematological parameters and gill parasite population of matrinxã Brycon cephalus (Günther, 1869) (Osteichthyes, Characidae) |
spellingShingle |
Transport with different benzocaine concentrations and its consequences on hematological parameters and gill parasite population of matrinxã Brycon cephalus (Günther, 1869) (Osteichthyes, Characidae) Carneiro, Paulo César Falanghe transporte estresse benzocaína matrinxã Brycon cephalus 2.00.00.00-6 Ciências Biológicas |
title_short |
Transport with different benzocaine concentrations and its consequences on hematological parameters and gill parasite population of matrinxã Brycon cephalus (Günther, 1869) (Osteichthyes, Characidae) |
title_full |
Transport with different benzocaine concentrations and its consequences on hematological parameters and gill parasite population of matrinxã Brycon cephalus (Günther, 1869) (Osteichthyes, Characidae) |
title_fullStr |
Transport with different benzocaine concentrations and its consequences on hematological parameters and gill parasite population of matrinxã Brycon cephalus (Günther, 1869) (Osteichthyes, Characidae) |
title_full_unstemmed |
Transport with different benzocaine concentrations and its consequences on hematological parameters and gill parasite population of matrinxã Brycon cephalus (Günther, 1869) (Osteichthyes, Characidae) |
title_sort |
Transport with different benzocaine concentrations and its consequences on hematological parameters and gill parasite population of matrinxã Brycon cephalus (Günther, 1869) (Osteichthyes, Characidae) |
author |
Carneiro, Paulo César Falanghe |
author_facet |
Carneiro, Paulo César Falanghe Urbinati, Elisabeth Criscuolo Martins, Maurício Laterça |
author_role |
author |
author2 |
Urbinati, Elisabeth Criscuolo Martins, Maurício Laterça |
author2_role |
author author |
dc.contributor.author.fl_str_mv |
Carneiro, Paulo César Falanghe Urbinati, Elisabeth Criscuolo Martins, Maurício Laterça |
dc.subject.por.fl_str_mv |
transporte estresse benzocaína matrinxã Brycon cephalus 2.00.00.00-6 Ciências Biológicas |
topic |
transporte estresse benzocaína matrinxã Brycon cephalus 2.00.00.00-6 Ciências Biológicas |
description |
The objective of this study was to evaluate the transport stressing effects and the different benzocaine concentrations on hematological parameters and parasite population of matrinxã Brycon cephalus (Osteichthyes, Characidae). Thirty fish (1.0 kg mean weight) were hauled in four 200-L plastic tanks for four hours, at different benzocaine concentrations (B0 = 0.0 g/L, B1 = 5.0 g/L, B2 = 10.0 g/L and B3 = 20 g/L). Before loading, blood and gills of 5 fish were collected. Other three samplings were performed after transport, and 24 and 96 hours later (recovery period). Blood cortisol, glucose, hematocrit, white cell differential count and the number of the gill parasite Piscinoodinium sp. were determined. Plasma cortisol increased in all treatments after transport, returning to initial levels 24 hours later. Blood glucose was also elevated after transport in all treatments, but only B0 and B1 fish recovered initial levels in 24 hours. B2 and B3 fish did not recover the initial levels until the end of the experimental period. After transport, the lymphocytes relative percentage decreased and the neuthrophils relative percentage increased, in all treatments. Ninety-six hours later, only B0 and B1 fish had recovered the initial levels of these parameters. The number of Piscinoodinium sp. on the gills of B3 fish was higher than in the other treatments 96 hours after transportation. The use of benzocaine did not mitigate stress responses and some negative effects were observed in fish transported with the highest concentrations. |
publishDate |
2008 |
dc.date.none.fl_str_mv |
2008-05-13 |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://www.periodicos.uem.br/ojs/index.php/ActaSciBiolSci/article/view/2359 10.4025/actascibiolsci.v24i0.2359 |
url |
http://www.periodicos.uem.br/ojs/index.php/ActaSciBiolSci/article/view/2359 |
identifier_str_mv |
10.4025/actascibiolsci.v24i0.2359 |
dc.language.iso.fl_str_mv |
por |
language |
por |
dc.relation.none.fl_str_mv |
http://www.periodicos.uem.br/ojs/index.php/ActaSciBiolSci/article/view/2359/2301 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
application/pdf |
dc.publisher.none.fl_str_mv |
Universidade Estadual De Maringá |
publisher.none.fl_str_mv |
Universidade Estadual De Maringá |
dc.source.none.fl_str_mv |
Acta Scientiarum. Biological Sciences; Vol 24 (2002); 555-560 Acta Scientiarum. Biological Sciences; v. 24 (2002); 555-560 1807-863X 1679-9283 reponame:Acta Scientiarum Biological Sciences instname:Universidade Estadual de Maringá (UEM) instacron:UEM |
instname_str |
Universidade Estadual de Maringá (UEM) |
instacron_str |
UEM |
institution |
UEM |
reponame_str |
Acta Scientiarum Biological Sciences |
collection |
Acta Scientiarum Biological Sciences |
repository.name.fl_str_mv |
Acta Scientiarum Biological Sciences - Universidade Estadual de Maringá (UEM) |
repository.mail.fl_str_mv |
||actabiol@uem.br |
_version_ |
1799317393094213632 |