Efficiency of methods for the detection of Didymella bryoniae associated with seeds of muskmelon hybrids - DOI: 10.4025/actasciagron.v31i3.360
Autor(a) principal: | |
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Data de Publicação: | 2009 |
Outros Autores: | , , , , |
Tipo de documento: | Artigo |
Idioma: | por |
Título da fonte: | Acta Scientiarum. Agronomy (Online) |
Texto Completo: | http://www.periodicos.uem.br/ojs/index.php/ActaSciAgron/article/view/360 |
Resumo: | Seeds are the primary inoculum source of Didymella bryoniae on cucurbits cultivated in plastic greenhouse. The objective of this work was to compare the effectiveness of methodologies used for the detection of this pathogen in muskmelon seeds. Four methodologies were evaluated: blotter test with seed freezing and whole seed analysis; blotter test without seed freezing with the analysis of seed parts (seed coat, perisperm, and cotyledons); test of seedling symptoms growing in sand; and test of seedling symptoms growing in the commercial substrate Plantmax®. For each test, 200 seeds of the hybrids Sunrise, Bonus II, and Prince Hakucho were used. The results of the blotter test did not show any D. bryoniae structure associated with seeds. The seedling test in sand did not present any visible disease symptom. However, the seedlings growing in commercial substrate presented gummy stem blight symptoms, with seed transmission rates of 52, 45, and 28%, for the hybrids Sunrise, Bonus II, and Prince Hakucho, respectively, showing that this was the most efficient method for detection. |
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Efficiency of methods for the detection of Didymella bryoniae associated with seeds of muskmelon hybrids - DOI: 10.4025/actasciagron.v31i3.360Eficiência de métodos para detecção de Didymella bryoniae associado a sementes de híbridos de meloeiros nobres - DOI: 10.4025/actasciagron.v31i3.360gummy stem blightCucumis melseed pathologypodridão gomosaCucumis melopatologia de sementes5.01.02.00-1 FitossanidadeSeeds are the primary inoculum source of Didymella bryoniae on cucurbits cultivated in plastic greenhouse. The objective of this work was to compare the effectiveness of methodologies used for the detection of this pathogen in muskmelon seeds. Four methodologies were evaluated: blotter test with seed freezing and whole seed analysis; blotter test without seed freezing with the analysis of seed parts (seed coat, perisperm, and cotyledons); test of seedling symptoms growing in sand; and test of seedling symptoms growing in the commercial substrate Plantmax®. For each test, 200 seeds of the hybrids Sunrise, Bonus II, and Prince Hakucho were used. The results of the blotter test did not show any D. bryoniae structure associated with seeds. The seedling test in sand did not present any visible disease symptom. However, the seedlings growing in commercial substrate presented gummy stem blight symptoms, with seed transmission rates of 52, 45, and 28%, for the hybrids Sunrise, Bonus II, and Prince Hakucho, respectively, showing that this was the most efficient method for detection.As sementes constituem a principal fonte de inóculo primário de Didymella bryoniae em cucurbitáceas cultivadas em ambiente protegido. Este trabalho objetivou comparar a sensibilidade de metodologias para avaliar a qualidade sanitária de sementes de meloeiro nobre quant o a D. bryoniae. Empregaram-se quatro metodologias para efetuar a análise de sanidade: teste em papel-filtro, com congelamento, para análise de sementes inteiras e, sem congelamento, para análise de sementes divididas (casca e perisperma+embrião); teste de sintomas em plântulas em substrato areia e teste de sintomas em plântulas em substrato comercial tipo Plantmax®. Para cada teste foram utilizadas 200 sementes de cada um dos híbridos Sunrise, Bonus II e Prince Hakucho. Os resultados indicaram que no teste em papel-filtro não foram encontradas quaisquer estruturas de D. bryoniae associadas às sementes inteiras e às sementes divididas de nenhum dos três híbridos de meloeiro. Pelo teste de sintoma em plântulas em areia não ocorreu sintomatologia visível causada por D. bryoniae. Já em substrato comercial Plantmax®, constatou-se elevado índice de transmissão de D. bryoniae, com 52, 45 e 28% de plantas sintomáticas para os híbridos Sunrise, Bonus II e Prince Hakucho, respectivamente, sendo este teste o mais eficiente.Universidade Estadual de Maringá2009-06-23info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionapplication/pdfhttp://www.periodicos.uem.br/ojs/index.php/ActaSciAgron/article/view/36010.4025/actasciagron.v31i3.360Acta Scientiarum. Agronomy; Vol 31 No 3 (2009); 397-402Acta Scientiarum. Agronomy; v. 31 n. 3 (2009); 397-4021807-86211679-9275reponame:Acta Scientiarum. Agronomy (Online)instname:Universidade Estadual de Maringá (UEM)instacron:UEMporhttp://www.periodicos.uem.br/ojs/index.php/ActaSciAgron/article/view/360/360Gasparotto, FrancielliVida, João BatistaTessmann, Dauri JoséBonaldo, Solange MariaAguiar, Ronilda LanaPenharbel, Marcelo Paesinfo:eu-repo/semantics/openAccess2022-11-23T18:37:04Zoai:periodicos.uem.br/ojs:article/360Revistahttp://www.periodicos.uem.br/ojs/index.php/ActaSciAgronPUBhttp://www.periodicos.uem.br/ojs/index.php/ActaSciAgron/oaiactaagron@uem.br||actaagron@uem.br|| edamasio@uem.br1807-86211679-9275opendoar:2022-11-23T18:37:04Acta Scientiarum. Agronomy (Online) - Universidade Estadual de Maringá (UEM)false |
dc.title.none.fl_str_mv |
Efficiency of methods for the detection of Didymella bryoniae associated with seeds of muskmelon hybrids - DOI: 10.4025/actasciagron.v31i3.360 Eficiência de métodos para detecção de Didymella bryoniae associado a sementes de híbridos de meloeiros nobres - DOI: 10.4025/actasciagron.v31i3.360 |
title |
Efficiency of methods for the detection of Didymella bryoniae associated with seeds of muskmelon hybrids - DOI: 10.4025/actasciagron.v31i3.360 |
spellingShingle |
Efficiency of methods for the detection of Didymella bryoniae associated with seeds of muskmelon hybrids - DOI: 10.4025/actasciagron.v31i3.360 Gasparotto, Francielli gummy stem blight Cucumis mel seed pathology podridão gomosa Cucumis melo patologia de sementes 5.01.02.00-1 Fitossanidade |
title_short |
Efficiency of methods for the detection of Didymella bryoniae associated with seeds of muskmelon hybrids - DOI: 10.4025/actasciagron.v31i3.360 |
title_full |
Efficiency of methods for the detection of Didymella bryoniae associated with seeds of muskmelon hybrids - DOI: 10.4025/actasciagron.v31i3.360 |
title_fullStr |
Efficiency of methods for the detection of Didymella bryoniae associated with seeds of muskmelon hybrids - DOI: 10.4025/actasciagron.v31i3.360 |
title_full_unstemmed |
Efficiency of methods for the detection of Didymella bryoniae associated with seeds of muskmelon hybrids - DOI: 10.4025/actasciagron.v31i3.360 |
title_sort |
Efficiency of methods for the detection of Didymella bryoniae associated with seeds of muskmelon hybrids - DOI: 10.4025/actasciagron.v31i3.360 |
author |
Gasparotto, Francielli |
author_facet |
Gasparotto, Francielli Vida, João Batista Tessmann, Dauri José Bonaldo, Solange Maria Aguiar, Ronilda Lana Penharbel, Marcelo Paes |
author_role |
author |
author2 |
Vida, João Batista Tessmann, Dauri José Bonaldo, Solange Maria Aguiar, Ronilda Lana Penharbel, Marcelo Paes |
author2_role |
author author author author author |
dc.contributor.author.fl_str_mv |
Gasparotto, Francielli Vida, João Batista Tessmann, Dauri José Bonaldo, Solange Maria Aguiar, Ronilda Lana Penharbel, Marcelo Paes |
dc.subject.por.fl_str_mv |
gummy stem blight Cucumis mel seed pathology podridão gomosa Cucumis melo patologia de sementes 5.01.02.00-1 Fitossanidade |
topic |
gummy stem blight Cucumis mel seed pathology podridão gomosa Cucumis melo patologia de sementes 5.01.02.00-1 Fitossanidade |
description |
Seeds are the primary inoculum source of Didymella bryoniae on cucurbits cultivated in plastic greenhouse. The objective of this work was to compare the effectiveness of methodologies used for the detection of this pathogen in muskmelon seeds. Four methodologies were evaluated: blotter test with seed freezing and whole seed analysis; blotter test without seed freezing with the analysis of seed parts (seed coat, perisperm, and cotyledons); test of seedling symptoms growing in sand; and test of seedling symptoms growing in the commercial substrate Plantmax®. For each test, 200 seeds of the hybrids Sunrise, Bonus II, and Prince Hakucho were used. The results of the blotter test did not show any D. bryoniae structure associated with seeds. The seedling test in sand did not present any visible disease symptom. However, the seedlings growing in commercial substrate presented gummy stem blight symptoms, with seed transmission rates of 52, 45, and 28%, for the hybrids Sunrise, Bonus II, and Prince Hakucho, respectively, showing that this was the most efficient method for detection. |
publishDate |
2009 |
dc.date.none.fl_str_mv |
2009-06-23 |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://www.periodicos.uem.br/ojs/index.php/ActaSciAgron/article/view/360 10.4025/actasciagron.v31i3.360 |
url |
http://www.periodicos.uem.br/ojs/index.php/ActaSciAgron/article/view/360 |
identifier_str_mv |
10.4025/actasciagron.v31i3.360 |
dc.language.iso.fl_str_mv |
por |
language |
por |
dc.relation.none.fl_str_mv |
http://www.periodicos.uem.br/ojs/index.php/ActaSciAgron/article/view/360/360 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
application/pdf |
dc.publisher.none.fl_str_mv |
Universidade Estadual de Maringá |
publisher.none.fl_str_mv |
Universidade Estadual de Maringá |
dc.source.none.fl_str_mv |
Acta Scientiarum. Agronomy; Vol 31 No 3 (2009); 397-402 Acta Scientiarum. Agronomy; v. 31 n. 3 (2009); 397-402 1807-8621 1679-9275 reponame:Acta Scientiarum. Agronomy (Online) instname:Universidade Estadual de Maringá (UEM) instacron:UEM |
instname_str |
Universidade Estadual de Maringá (UEM) |
instacron_str |
UEM |
institution |
UEM |
reponame_str |
Acta Scientiarum. Agronomy (Online) |
collection |
Acta Scientiarum. Agronomy (Online) |
repository.name.fl_str_mv |
Acta Scientiarum. Agronomy (Online) - Universidade Estadual de Maringá (UEM) |
repository.mail.fl_str_mv |
actaagron@uem.br||actaagron@uem.br|| edamasio@uem.br |
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1799305902123122688 |