Use of activated charcoal and ion-exchange resin to cleaN up and concentrate enzymes in extracts from biodegraded wood - 10.4025/actascitechnol.v32i4.5671

Detalhes bibliográficos
Autor(a) principal: Carvalho, Walter
Data de Publicação: 2010
Outros Autores: Ferraz, André, Milagres, Adriane Maria Ferreira
Tipo de documento: Artigo
Idioma: por
Título da fonte: Acta scientiarum. Technology (Online)
Texto Completo: http://www.periodicos.uem.br/ojs/index.php/ActaSciTechnol/article/view/5671
Resumo: Ceriporiopsis subvermispora was used for the biodegradation of Eucalyptus grandis chips in the presence or absence of co-substrates (glucose and corn steep liquor) during 7, 14 and 28 days. Afterwards, the biodegraded chips were extracted with 50 mM sodium acetate buffer (pH 5.5) supplemented with 0.01% Tween 60. High activities of manganese peroxidases (MnPs) were observed in all the extracts, both in the absence (430, 765 and 896 UI kg-1, respectively) and in the presence of co-substrates (1,013; 2,066 and 2,323 UI kg-1, respectively). The extracts presented a high ratio between absorbances at 280 and 405 nm, indicating a strong abundance of aromatic compounds derived from lignin over heme-peroxidases. Adsorption into activated charcoal showed to be an adequate strategy to reduce the absorbance at 280 nm in all the extracts. Moreover, it allowed to maximize the capacity of an anion exchange resin bed (DEAE-Sepharose) used to concentrate the MnPs present in the extracts. It was concluded that the use of activated charcoal followed by adsorption into DEAE Sepharose is a strategy that can be used to concentrate MnPs in extracts obtained during the biodegradation of E. grandis by C. subvermispora.
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spelling Use of activated charcoal and ion-exchange resin to cleaN up and concentrate enzymes in extracts from biodegraded wood - 10.4025/actascitechnol.v32i4.5671Uso de carvão ativado e resina de troca iônica para limpeza e concentração de enzimas em extratos de madeira biodegradada - 10.4025/actascitechnol.v32i4.5671Ceriporiopsis subvermisporaEucalyptus grandisManganese peroxidasesCeriporiopsis subvermisporaEucalyptus grandismanganês peroxidasesmicrobiologia aplicadaCeriporiopsis subvermispora was used for the biodegradation of Eucalyptus grandis chips in the presence or absence of co-substrates (glucose and corn steep liquor) during 7, 14 and 28 days. Afterwards, the biodegraded chips were extracted with 50 mM sodium acetate buffer (pH 5.5) supplemented with 0.01% Tween 60. High activities of manganese peroxidases (MnPs) were observed in all the extracts, both in the absence (430, 765 and 896 UI kg-1, respectively) and in the presence of co-substrates (1,013; 2,066 and 2,323 UI kg-1, respectively). The extracts presented a high ratio between absorbances at 280 and 405 nm, indicating a strong abundance of aromatic compounds derived from lignin over heme-peroxidases. Adsorption into activated charcoal showed to be an adequate strategy to reduce the absorbance at 280 nm in all the extracts. Moreover, it allowed to maximize the capacity of an anion exchange resin bed (DEAE-Sepharose) used to concentrate the MnPs present in the extracts. It was concluded that the use of activated charcoal followed by adsorption into DEAE Sepharose is a strategy that can be used to concentrate MnPs in extracts obtained during the biodegradation of E. grandis by C. subvermispora.Ceriporiopsis subvermispora foi utilizado para a biodegradação de cavacos de Eucalyptus grandis na presença ou ausência de cossubstratos (glicose e milhocina) durante 7, 14 e 28 dias. Os cavacos obtidos em cada um desses períodos foram extraídos com tampão acetato de sódio 50 mM (pH 5,5) adicionado de 0,01% de Tween 60. A atividade de manganês peroxidases (MnPs) nos extratos foi marcante ao longo de todo o período de biodegradação, tanto na ausência (430, 765 e 896 UI kg-1, respectivamente) quanto na presença de cossubstratos (1.013, 2.066 e 2.323 UI kg-1, respectivamente). Os extratos apresentaram elevadas relações entre absorbância a 280 e a 405 nm, indicando forte abundância de compostos aromáticos derivados da lignina em relação à heme-peroxidases. A adsorção em carvão ativado se mostrou uma estratégia adequada para reduzir a absorbância a 280 nm em todos os extratos. Além disso, permitiu maximizar a capacidade de um leito de resina trocadora de ânions (DEAE Sepharose), utilizado para concentrar as MnPs presentes nos referidos extratos. Concluiu-se que o uso de carvão ativado seguido de adsorção em DEAE Sepharose é uma estratégia que pode ser utilizada para concentrar MnPs em extratos obtidos durante a biodegradação de E. grandis por C. subvermispora.Universidade Estadual De Maringá2010-12-02info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionprodução, purificação e caracterização de enzimasapplication/pdfhttp://www.periodicos.uem.br/ojs/index.php/ActaSciTechnol/article/view/567110.4025/actascitechnol.v32i4.5671Acta Scientiarum. Technology; Vol 32 No 4 (2010); 347-354Acta Scientiarum. Technology; v. 32 n. 4 (2010); 347-3541806-25631807-8664reponame:Acta scientiarum. Technology (Online)instname:Universidade Estadual de Maringá (UEM)instacron:UEMporhttp://www.periodicos.uem.br/ojs/index.php/ActaSciTechnol/article/view/5671/5671Carvalho, WalterFerraz, AndréMilagres, Adriane Maria Ferreirainfo:eu-repo/semantics/openAccess2024-05-17T13:03:05Zoai:periodicos.uem.br/ojs:article/5671Revistahttps://www.periodicos.uem.br/ojs/index.php/ActaSciTechnol/indexPUBhttps://www.periodicos.uem.br/ojs/index.php/ActaSciTechnol/oai||actatech@uem.br1807-86641806-2563opendoar:2024-05-17T13:03:05Acta scientiarum. Technology (Online) - Universidade Estadual de Maringá (UEM)false
dc.title.none.fl_str_mv Use of activated charcoal and ion-exchange resin to cleaN up and concentrate enzymes in extracts from biodegraded wood - 10.4025/actascitechnol.v32i4.5671
Uso de carvão ativado e resina de troca iônica para limpeza e concentração de enzimas em extratos de madeira biodegradada - 10.4025/actascitechnol.v32i4.5671
title Use of activated charcoal and ion-exchange resin to cleaN up and concentrate enzymes in extracts from biodegraded wood - 10.4025/actascitechnol.v32i4.5671
spellingShingle Use of activated charcoal and ion-exchange resin to cleaN up and concentrate enzymes in extracts from biodegraded wood - 10.4025/actascitechnol.v32i4.5671
Carvalho, Walter
Ceriporiopsis subvermispora
Eucalyptus grandis
Manganese peroxidases
Ceriporiopsis subvermispora
Eucalyptus grandis
manganês peroxidases
microbiologia aplicada
title_short Use of activated charcoal and ion-exchange resin to cleaN up and concentrate enzymes in extracts from biodegraded wood - 10.4025/actascitechnol.v32i4.5671
title_full Use of activated charcoal and ion-exchange resin to cleaN up and concentrate enzymes in extracts from biodegraded wood - 10.4025/actascitechnol.v32i4.5671
title_fullStr Use of activated charcoal and ion-exchange resin to cleaN up and concentrate enzymes in extracts from biodegraded wood - 10.4025/actascitechnol.v32i4.5671
title_full_unstemmed Use of activated charcoal and ion-exchange resin to cleaN up and concentrate enzymes in extracts from biodegraded wood - 10.4025/actascitechnol.v32i4.5671
title_sort Use of activated charcoal and ion-exchange resin to cleaN up and concentrate enzymes in extracts from biodegraded wood - 10.4025/actascitechnol.v32i4.5671
author Carvalho, Walter
author_facet Carvalho, Walter
Ferraz, André
Milagres, Adriane Maria Ferreira
author_role author
author2 Ferraz, André
Milagres, Adriane Maria Ferreira
author2_role author
author
dc.contributor.author.fl_str_mv Carvalho, Walter
Ferraz, André
Milagres, Adriane Maria Ferreira
dc.subject.por.fl_str_mv Ceriporiopsis subvermispora
Eucalyptus grandis
Manganese peroxidases
Ceriporiopsis subvermispora
Eucalyptus grandis
manganês peroxidases
microbiologia aplicada
topic Ceriporiopsis subvermispora
Eucalyptus grandis
Manganese peroxidases
Ceriporiopsis subvermispora
Eucalyptus grandis
manganês peroxidases
microbiologia aplicada
description Ceriporiopsis subvermispora was used for the biodegradation of Eucalyptus grandis chips in the presence or absence of co-substrates (glucose and corn steep liquor) during 7, 14 and 28 days. Afterwards, the biodegraded chips were extracted with 50 mM sodium acetate buffer (pH 5.5) supplemented with 0.01% Tween 60. High activities of manganese peroxidases (MnPs) were observed in all the extracts, both in the absence (430, 765 and 896 UI kg-1, respectively) and in the presence of co-substrates (1,013; 2,066 and 2,323 UI kg-1, respectively). The extracts presented a high ratio between absorbances at 280 and 405 nm, indicating a strong abundance of aromatic compounds derived from lignin over heme-peroxidases. Adsorption into activated charcoal showed to be an adequate strategy to reduce the absorbance at 280 nm in all the extracts. Moreover, it allowed to maximize the capacity of an anion exchange resin bed (DEAE-Sepharose) used to concentrate the MnPs present in the extracts. It was concluded that the use of activated charcoal followed by adsorption into DEAE Sepharose is a strategy that can be used to concentrate MnPs in extracts obtained during the biodegradation of E. grandis by C. subvermispora.
publishDate 2010
dc.date.none.fl_str_mv 2010-12-02
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
produção, purificação e caracterização de enzimas
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://www.periodicos.uem.br/ojs/index.php/ActaSciTechnol/article/view/5671
10.4025/actascitechnol.v32i4.5671
url http://www.periodicos.uem.br/ojs/index.php/ActaSciTechnol/article/view/5671
identifier_str_mv 10.4025/actascitechnol.v32i4.5671
dc.language.iso.fl_str_mv por
language por
dc.relation.none.fl_str_mv http://www.periodicos.uem.br/ojs/index.php/ActaSciTechnol/article/view/5671/5671
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Universidade Estadual De Maringá
publisher.none.fl_str_mv Universidade Estadual De Maringá
dc.source.none.fl_str_mv Acta Scientiarum. Technology; Vol 32 No 4 (2010); 347-354
Acta Scientiarum. Technology; v. 32 n. 4 (2010); 347-354
1806-2563
1807-8664
reponame:Acta scientiarum. Technology (Online)
instname:Universidade Estadual de Maringá (UEM)
instacron:UEM
instname_str Universidade Estadual de Maringá (UEM)
instacron_str UEM
institution UEM
reponame_str Acta scientiarum. Technology (Online)
collection Acta scientiarum. Technology (Online)
repository.name.fl_str_mv Acta scientiarum. Technology (Online) - Universidade Estadual de Maringá (UEM)
repository.mail.fl_str_mv ||actatech@uem.br
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