Effect of initial pH in levan production by Zymomonas mobilis immobilized in sodium alginate
Autor(a) principal: | |
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Data de Publicação: | 2014 |
Outros Autores: | , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Acta scientiarum. Technology (Online) |
Texto Completo: | http://www.periodicos.uem.br/ojs/index.php/ActaSciTechnol/article/view/18372 |
Resumo: | Zymomonas mobilis was immobilized using a cell suspension fixed to 8.6 x 107 CFU mL-1 by spectrophotometry. This biomass was suspended in sodium alginate solution (3%) that was dropped with a hypodermic syringe into 0.2 M calcium chloride solution. Was test two initial pH of fermentation medium (4 and 5) and different sucrose concentrations 15, 20, 25, 30 and 35% at 30˚C, without stirring for 24, 48, 72 and 96 hours. The levan production to pH 4 was high in sucrose 25% for 24 (16.51 g L-1) and 48 (15.31 g L-1) hours. The best values obtained to pH 5 was in sucrose 35% during 48 (22.39 g L-1) and 96 (23.5 g L-1) hours, respectively. The maximum levan yield was 40.8% and 22.47% in sucrose 15% to pH 4 and 5, respectively. Substrate consumption to pH 4 was bigger in sucrose 15 (56.4%) and 20% (59.4%) and to pH 5 was in 25 (68.85%) and 35% (64.64%). In relation to immobilization efficiency, Zymomonas mobilis showed high adhesion and colonization in support, indicated by cell growth increased from 107 to 109 CFU mL-1 during fermentation time. |
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Acta scientiarum. Technology (Online) |
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Effect of initial pH in levan production by Zymomonas mobilis immobilized in sodium alginateentrapment cellbatch fermentationbiopolymerCência e Tecnologia de AlimentosZymomonas mobilis was immobilized using a cell suspension fixed to 8.6 x 107 CFU mL-1 by spectrophotometry. This biomass was suspended in sodium alginate solution (3%) that was dropped with a hypodermic syringe into 0.2 M calcium chloride solution. Was test two initial pH of fermentation medium (4 and 5) and different sucrose concentrations 15, 20, 25, 30 and 35% at 30˚C, without stirring for 24, 48, 72 and 96 hours. The levan production to pH 4 was high in sucrose 25% for 24 (16.51 g L-1) and 48 (15.31 g L-1) hours. The best values obtained to pH 5 was in sucrose 35% during 48 (22.39 g L-1) and 96 (23.5 g L-1) hours, respectively. The maximum levan yield was 40.8% and 22.47% in sucrose 15% to pH 4 and 5, respectively. Substrate consumption to pH 4 was bigger in sucrose 15 (56.4%) and 20% (59.4%) and to pH 5 was in 25 (68.85%) and 35% (64.64%). In relation to immobilization efficiency, Zymomonas mobilis showed high adhesion and colonization in support, indicated by cell growth increased from 107 to 109 CFU mL-1 during fermentation time. Universidade Estadual De Maringá2014-04-04info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionapplication/pdfhttp://www.periodicos.uem.br/ojs/index.php/ActaSciTechnol/article/view/1837210.4025/actascitechnol.v36i2.18372Acta Scientiarum. Technology; Vol 36 No 2 (2014); 349-354Acta Scientiarum. Technology; v. 36 n. 2 (2014); 349-3541806-25631807-8664reponame:Acta scientiarum. Technology (Online)instname:Universidade Estadual de Maringá (UEM)instacron:UEMenghttp://www.periodicos.uem.br/ojs/index.php/ActaSciTechnol/article/view/18372/12669Santos, Vidiany Aparecida QueirozBianchi, Vanildo Luiz DelGarcia-Cruz, Crispin Humbertoinfo:eu-repo/semantics/openAccess2014-04-04T15:34:15Zoai:periodicos.uem.br/ojs:article/18372Revistahttps://www.periodicos.uem.br/ojs/index.php/ActaSciTechnol/indexPUBhttps://www.periodicos.uem.br/ojs/index.php/ActaSciTechnol/oai||actatech@uem.br1807-86641806-2563opendoar:2014-04-04T15:34:15Acta scientiarum. Technology (Online) - Universidade Estadual de Maringá (UEM)false |
dc.title.none.fl_str_mv |
Effect of initial pH in levan production by Zymomonas mobilis immobilized in sodium alginate |
title |
Effect of initial pH in levan production by Zymomonas mobilis immobilized in sodium alginate |
spellingShingle |
Effect of initial pH in levan production by Zymomonas mobilis immobilized in sodium alginate Santos, Vidiany Aparecida Queiroz entrapment cell batch fermentation biopolymer Cência e Tecnologia de Alimentos |
title_short |
Effect of initial pH in levan production by Zymomonas mobilis immobilized in sodium alginate |
title_full |
Effect of initial pH in levan production by Zymomonas mobilis immobilized in sodium alginate |
title_fullStr |
Effect of initial pH in levan production by Zymomonas mobilis immobilized in sodium alginate |
title_full_unstemmed |
Effect of initial pH in levan production by Zymomonas mobilis immobilized in sodium alginate |
title_sort |
Effect of initial pH in levan production by Zymomonas mobilis immobilized in sodium alginate |
author |
Santos, Vidiany Aparecida Queiroz |
author_facet |
Santos, Vidiany Aparecida Queiroz Bianchi, Vanildo Luiz Del Garcia-Cruz, Crispin Humberto |
author_role |
author |
author2 |
Bianchi, Vanildo Luiz Del Garcia-Cruz, Crispin Humberto |
author2_role |
author author |
dc.contributor.author.fl_str_mv |
Santos, Vidiany Aparecida Queiroz Bianchi, Vanildo Luiz Del Garcia-Cruz, Crispin Humberto |
dc.subject.por.fl_str_mv |
entrapment cell batch fermentation biopolymer Cência e Tecnologia de Alimentos |
topic |
entrapment cell batch fermentation biopolymer Cência e Tecnologia de Alimentos |
description |
Zymomonas mobilis was immobilized using a cell suspension fixed to 8.6 x 107 CFU mL-1 by spectrophotometry. This biomass was suspended in sodium alginate solution (3%) that was dropped with a hypodermic syringe into 0.2 M calcium chloride solution. Was test two initial pH of fermentation medium (4 and 5) and different sucrose concentrations 15, 20, 25, 30 and 35% at 30˚C, without stirring for 24, 48, 72 and 96 hours. The levan production to pH 4 was high in sucrose 25% for 24 (16.51 g L-1) and 48 (15.31 g L-1) hours. The best values obtained to pH 5 was in sucrose 35% during 48 (22.39 g L-1) and 96 (23.5 g L-1) hours, respectively. The maximum levan yield was 40.8% and 22.47% in sucrose 15% to pH 4 and 5, respectively. Substrate consumption to pH 4 was bigger in sucrose 15 (56.4%) and 20% (59.4%) and to pH 5 was in 25 (68.85%) and 35% (64.64%). In relation to immobilization efficiency, Zymomonas mobilis showed high adhesion and colonization in support, indicated by cell growth increased from 107 to 109 CFU mL-1 during fermentation time. |
publishDate |
2014 |
dc.date.none.fl_str_mv |
2014-04-04 |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://www.periodicos.uem.br/ojs/index.php/ActaSciTechnol/article/view/18372 10.4025/actascitechnol.v36i2.18372 |
url |
http://www.periodicos.uem.br/ojs/index.php/ActaSciTechnol/article/view/18372 |
identifier_str_mv |
10.4025/actascitechnol.v36i2.18372 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
http://www.periodicos.uem.br/ojs/index.php/ActaSciTechnol/article/view/18372/12669 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
application/pdf |
dc.publisher.none.fl_str_mv |
Universidade Estadual De Maringá |
publisher.none.fl_str_mv |
Universidade Estadual De Maringá |
dc.source.none.fl_str_mv |
Acta Scientiarum. Technology; Vol 36 No 2 (2014); 349-354 Acta Scientiarum. Technology; v. 36 n. 2 (2014); 349-354 1806-2563 1807-8664 reponame:Acta scientiarum. Technology (Online) instname:Universidade Estadual de Maringá (UEM) instacron:UEM |
instname_str |
Universidade Estadual de Maringá (UEM) |
instacron_str |
UEM |
institution |
UEM |
reponame_str |
Acta scientiarum. Technology (Online) |
collection |
Acta scientiarum. Technology (Online) |
repository.name.fl_str_mv |
Acta scientiarum. Technology (Online) - Universidade Estadual de Maringá (UEM) |
repository.mail.fl_str_mv |
||actatech@uem.br |
_version_ |
1799315335020544000 |