Molecular techniques applied to food microbiology: a review - DOI: 10.4025/actascitechnol.v30i1.3245

Detalhes bibliográficos
Autor(a) principal: Gandra, Eliezer Ávila
Data de Publicação: 2008
Outros Autores: Gandra, Tatiane Kuka Valente, Mello, Willians Sebastião de, Godoi, Huana da Silva
Tipo de documento: Artigo
Idioma: por
Título da fonte: Acta scientiarum. Technology (Online)
Texto Completo: http://www.periodicos.uem.br/ojs/index.php/ActaSciTechnol/article/view/3245
Resumo: Beginning in the 1980s, molecular techniques became an alternative to the traditionally used phenotypic methods in food microbiology. With the advent of the polymerase chain reaction technique, this substitution was speed up. This article had as objective to review the main molecular techniques used as tools in food microbiology, from plasmidial profile analysis to contemporary techniques such as the real-time PCR. The characteristics, advantages and disadvantages of these techniques are discussed, by evaluating the potential of these techniques to overcome the limitations of traditional techniques.
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spelling Molecular techniques applied to food microbiology: a review - DOI: 10.4025/actascitechnol.v30i1.3245Técnicas moleculares aplicadas à microbiologia de alimentos - DOI: 10.4025/actascitechnol.v30i1.3245REAPFGEribotypingRAPDRT-PCRmultiplex PCRREAPFGEribotipagemRAPDRT-PCRmultiplex PCR5.07.00.00-6 Ciência e Tecnologia de AlimentosBeginning in the 1980s, molecular techniques became an alternative to the traditionally used phenotypic methods in food microbiology. With the advent of the polymerase chain reaction technique, this substitution was speed up. This article had as objective to review the main molecular techniques used as tools in food microbiology, from plasmidial profile analysis to contemporary techniques such as the real-time PCR. The characteristics, advantages and disadvantages of these techniques are discussed, by evaluating the potential of these techniques to overcome the limitations of traditional techniques.A partir da década de 80, as técnicas moleculares começaram a ser utilizadas como uma alternativa aos métodos fenotípicos, tradicionalmente, utilizados em microbiologia de alimentos. Foi acelerada esta substituição com advento da descoberta da reação em cadeia da polimerase (polymerase chain reaction – PCR). Este artigo tem por objetivo revisar as principais técnicas moleculares utilizadas como ferramentas na microbiologia de alimentos, desde as, inicialmente, desenvolvidas, como a análise do perfil plasmidial, até as mais contemporâneas como o PCR em tempo real, discutindo as características, vantagens e desvantagens destas técnicas, avaliando a potencialidade destas para suprir as limitações das técnicas tradicionais.Universidade Estadual De Maringá2008-05-08info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionapplication/pdfhttp://www.periodicos.uem.br/ojs/index.php/ActaSciTechnol/article/view/324510.4025/actascitechnol.v30i1.3245Acta Scientiarum. Technology; Vol 30 No 1 (2008); 109-118Acta Scientiarum. Technology; v. 30 n. 1 (2008); 109-1181806-25631807-8664reponame:Acta scientiarum. Technology (Online)instname:Universidade Estadual de Maringá (UEM)instacron:UEMporhttp://www.periodicos.uem.br/ojs/index.php/ActaSciTechnol/article/view/3245/1939Gandra, Eliezer ÁvilaGandra, Tatiane Kuka ValenteMello, Willians Sebastião deGodoi, Huana da Silvainfo:eu-repo/semantics/openAccess2024-05-17T13:03:00Zoai:periodicos.uem.br/ojs:article/3245Revistahttps://www.periodicos.uem.br/ojs/index.php/ActaSciTechnol/indexPUBhttps://www.periodicos.uem.br/ojs/index.php/ActaSciTechnol/oai||actatech@uem.br1807-86641806-2563opendoar:2024-05-17T13:03Acta scientiarum. Technology (Online) - Universidade Estadual de Maringá (UEM)false
dc.title.none.fl_str_mv Molecular techniques applied to food microbiology: a review - DOI: 10.4025/actascitechnol.v30i1.3245
Técnicas moleculares aplicadas à microbiologia de alimentos - DOI: 10.4025/actascitechnol.v30i1.3245
title Molecular techniques applied to food microbiology: a review - DOI: 10.4025/actascitechnol.v30i1.3245
spellingShingle Molecular techniques applied to food microbiology: a review - DOI: 10.4025/actascitechnol.v30i1.3245
Gandra, Eliezer Ávila
REA
PFGE
ribotyping
RAPD
RT-PCR
multiplex PCR
REA
PFGE
ribotipagem
RAPD
RT-PCR
multiplex PCR
5.07.00.00-6 Ciência e Tecnologia de Alimentos
title_short Molecular techniques applied to food microbiology: a review - DOI: 10.4025/actascitechnol.v30i1.3245
title_full Molecular techniques applied to food microbiology: a review - DOI: 10.4025/actascitechnol.v30i1.3245
title_fullStr Molecular techniques applied to food microbiology: a review - DOI: 10.4025/actascitechnol.v30i1.3245
title_full_unstemmed Molecular techniques applied to food microbiology: a review - DOI: 10.4025/actascitechnol.v30i1.3245
title_sort Molecular techniques applied to food microbiology: a review - DOI: 10.4025/actascitechnol.v30i1.3245
author Gandra, Eliezer Ávila
author_facet Gandra, Eliezer Ávila
Gandra, Tatiane Kuka Valente
Mello, Willians Sebastião de
Godoi, Huana da Silva
author_role author
author2 Gandra, Tatiane Kuka Valente
Mello, Willians Sebastião de
Godoi, Huana da Silva
author2_role author
author
author
dc.contributor.author.fl_str_mv Gandra, Eliezer Ávila
Gandra, Tatiane Kuka Valente
Mello, Willians Sebastião de
Godoi, Huana da Silva
dc.subject.por.fl_str_mv REA
PFGE
ribotyping
RAPD
RT-PCR
multiplex PCR
REA
PFGE
ribotipagem
RAPD
RT-PCR
multiplex PCR
5.07.00.00-6 Ciência e Tecnologia de Alimentos
topic REA
PFGE
ribotyping
RAPD
RT-PCR
multiplex PCR
REA
PFGE
ribotipagem
RAPD
RT-PCR
multiplex PCR
5.07.00.00-6 Ciência e Tecnologia de Alimentos
description Beginning in the 1980s, molecular techniques became an alternative to the traditionally used phenotypic methods in food microbiology. With the advent of the polymerase chain reaction technique, this substitution was speed up. This article had as objective to review the main molecular techniques used as tools in food microbiology, from plasmidial profile analysis to contemporary techniques such as the real-time PCR. The characteristics, advantages and disadvantages of these techniques are discussed, by evaluating the potential of these techniques to overcome the limitations of traditional techniques.
publishDate 2008
dc.date.none.fl_str_mv 2008-05-08
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://www.periodicos.uem.br/ojs/index.php/ActaSciTechnol/article/view/3245
10.4025/actascitechnol.v30i1.3245
url http://www.periodicos.uem.br/ojs/index.php/ActaSciTechnol/article/view/3245
identifier_str_mv 10.4025/actascitechnol.v30i1.3245
dc.language.iso.fl_str_mv por
language por
dc.relation.none.fl_str_mv http://www.periodicos.uem.br/ojs/index.php/ActaSciTechnol/article/view/3245/1939
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Universidade Estadual De Maringá
publisher.none.fl_str_mv Universidade Estadual De Maringá
dc.source.none.fl_str_mv Acta Scientiarum. Technology; Vol 30 No 1 (2008); 109-118
Acta Scientiarum. Technology; v. 30 n. 1 (2008); 109-118
1806-2563
1807-8664
reponame:Acta scientiarum. Technology (Online)
instname:Universidade Estadual de Maringá (UEM)
instacron:UEM
instname_str Universidade Estadual de Maringá (UEM)
instacron_str UEM
institution UEM
reponame_str Acta scientiarum. Technology (Online)
collection Acta scientiarum. Technology (Online)
repository.name.fl_str_mv Acta scientiarum. Technology (Online) - Universidade Estadual de Maringá (UEM)
repository.mail.fl_str_mv ||actatech@uem.br
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