Atividade antimicrobiana do extrato de semente de uva Vitis vinifera sobre biofilme de Enterococcus faecalis: análise com microscopia confocal

• Autor(a) principal: Fiallos, Nicole de Mello
• Data de Publicação: 2017
• Tipo de documento: Dissertação
• Idioma: por
• Título da fonte: Biblioteca Digital de Teses e Dissertações da UERJ
• Texto Completo: http://www.bdtd.uerj.br/handle/1/14218
• topic: Grape seed extract; Enterococcus faecalis; Dental biofilm; Root canal irrigants; Microscopy Confocal; Biofilme dentário; Extrato de semente de uva; Enterococcus faecalis; Irrigantes do Canal Radicular; Microscopia Confocal; CNPQ::CIENCIAS DA SAUDE::ODONTOLOGIA::ENDODONTIA

The limitations of chemical irrigants used in endodontics have encouraged further research on alternative substances to be used in endodontic treatment. Previous studies have suggested that grape seed extract (Vitis vinifera), an irrigant of natural origin, is effective against strains of Enterococcus faecalis. The aim of this study was to evaluate and quantify the degree of disinfection of dentin contaminated by E. faecalis biofilm after irrigation with Vitis vinifera grape seed extract 6,5% and to compare it with chlorhexidine (CHX) 2% and Sodium hypochlorite (NaOCl) 5,25% by confocal microscopy laser. Forty samples of dentin disks that will be contaminated with E. faecalis and incubated for a period of 21 days for biofilm formation were prepared. The samples were divided into different groups according to the irrigant tested after the period of contamination: NaOCl group, CHX group, Vitis vinifera group and control group . After irrigation, the dentin disks were stained with the fluorescent dye LIVE / DEAD BacLight and analyzed by laser confocal microscopy to determine the proportion of dead cells in the biofilm. The acquisition of the images and quantification of the biofilm was performed using the software LAS X. Normal distribution of the data was confirmed by Shapiro- Wilk test (p>0.05). Therefore, statistical analysis was performed using One-way analysis of variance. Post hoc pair-wise comparisons were performed using Tukey s test for multiple comparisons (P<0.05). NaOCl presented a higher amount of bacterial cells killed than the other irrigators (P <0.05). Irrigation with grape seed extract, Vitis vinifera, showed antimicrobial activity on the E. faecalis biofilm, being lower than that presented by NaOCl and higher than that presented by CHX.