Avaliação de metodologias para análise de DNA de amostras de restos mortais e construção de banco de dados de DNA mitocondrial da população do Estado do Rio de Janeiro

• Autor(a) principal: Silva, Márcia Teixeira Desidério da
• Data de Publicação: 2009
• Tipo de documento: Dissertação
• Idioma: por
• Título da fonte: Biblioteca Digital de Teses e Dissertações da UERJ
• Texto Completo: http://www.bdtd.uerj.br/handle/1/8822
• topic: Mitocondrial DNA; Human remains; Human identification; genética forense; antropologia forense; DNA mitocondrial; RIestos mortais; Identificação humana; CNPQ::CIENCIAS DA SAUDE::MEDICINA::MEDICINA LEGAL E DEONTOLOGIA

Analysis of mtDNA has been employed, recently, in Forensic Science, for identification of peoples and solves criminal cases. So has been used in genetic populations studies and had contributed for understand of process related with degree of mixture people and with occupation of different continents by populations humans. Mitochondrial DNA is present in many copies by cell. It is maternal inheritance and not suffer recombination. So, this cell is powerful tool in this area. Analysis of mitochondrial DNA is recommend when not exist more nuclear DNA or it be degraded as in forensic samples. One of problems find are the artifact encountered during the phases before and after sequencing where can obtained the bad quality sequence. The present study compared and optimized methods of mitochondrial DNA purification to samples human remains and in vivo in phases before and after sequencing. The methodology of analysis by mitochondrial DNA consist in extract it of biological sample, amplify the regions HVS-I and HVS-II of control region, sequence the products amplified and analysis after electroforesis capilar. The colagenase was tested with objective of value its efficiency in phase DNA extraction. In relation that fase, other objective was value the degree polymorphisms in regions HVS-I and HVS-II of mtDNA in samples of population of Rio de Janeiro to contribute for understand of flow genetics associated to formation of our population. As conclusion, the best methods of purification used was Centricon, follow by purification of amplifications products by Amershan and purification od products sequencing by Sephadex. It had fall efficiency of colagenase indicate the presence of probable inhibitors. In relation of data base, was encountered 95 polymorfisms distincts in region HVS-I and 52 in region HVS-II. The polimorfism more often in region HVS-I was 16223 (74,5%), while in region HVS-II was 263 (60%). It was encountered 92 differents haplotypes in region HVS-I and 42 in region HVS-II. It had lower rates of heteroplasmy. We can said that the population of Rio de Janeiro state, by maternal inheritance, was determined, in more portion, by Africans (50%), follow by Amerindians (30%) with small portion of Europeans (21%), showing the bigger miscegenation of our state.