Estudo dos constituintes químicos da espécie vegetal Manilkara subsericea

• Autor(a) principal: Gutierrez, Erika Del Pilar Munoz
• Data de Publicação: 2018
• Tipo de documento: Dissertação
• Idioma: por
• Título da fonte: Biblioteca Digital de Teses e Dissertações da UERJ
• Texto Completo: http://www.bdtd.uerj.br/handle/1/15838
• topic: Manilkara subsericea; squalene; lupeol; hydrocarbons; chemical composition; Manilkara subsericea; esqualeno; lupeol; hidrocarbonetos; composição química; CNPQ::CIENCIAS EXATAS E DA TERRA::QUIMICA::QUIMICA ORGANICA

The present work deals with the study of the chemical constituents of the plant species Manilkara subsericea, which is part of the Sapotaceae Family and is an endemic species of the Brazilian Atlantic Forest. The study covers Soxhlet Extraction using hexane and ethyl acetate as solvents; open column fractionation; evaluation by thin layer chromatography (TLC) on analytical and preparative scales; identification of compounds by High Resolution Gas Chromatography coupled to Mass Spectrometry (HRGC-MS) and 13C and 1H Nuclear Magnetic Resonance Spectroscopy (NMR). The presence of α/β-amiryn-derived triterpenes, in addition to α-Tocoferol, one of the isomers that make up vitamin E, was confirmed by the coinjection method of hexane fractions HG28 and HF32-Rf1 with their respective reference standards. The presence of squalene, one of the main precursors in the biosynthetic route of triterpenes, compounds with important biochemical activities and not yet reported in the literature for this species was also confirmed in the HF32 Rf1 fraction. Another compound detected for the first time in Manilkara subsericea was lupeol acetate, in the HG24-Rf1 fraction, bycomparing its mass spectrum with that of the National Institute of Standards and Technology (NIST) of the product precursor ratio by the Multiple Reaction Monitoring Method (MRM MS / MS) performed through Mass Spectrometry and the13C NMR spectroscopy study. A homologous series of saturated linear hydrocarbons (C19-C33) was also identified in the hexane fraction of the branches and leaves (HG03), with predominance of the odd compounds on the pairs, important for the taxonomic study of plant species, this series reported so far for the species Manilkara subsericea