Expressão de citocinas relacionadas à resposta Th17 nos tecidos gengival e intestinal de pacientes com periodontite e doença inflamatória intestinal

• Autor(a) principal: Côrtes, Ana Paula Morais Martins
• Data de Publicação: 2016
• Tipo de documento: Dissertação
• Idioma: por
• Título da fonte: Biblioteca Digital de Teses e Dissertações da UERJ
• Texto Completo: http://www.bdtd.uerj.br/handle/1/14216
• topic: Periodontitis; Inflammatory bowel disease; Cytokines; Th 17; IL-23; Periodontite; Doença inflamatória intestinal; Citocinas; Th17; IL-23; CNPQ::CIENCIAS DA SAUDE::ODONTOLOGIA::PERIODONTIA

Periodontitis and inflammatory bowel disease (IBD) result from an excessive immune response initiated or not by a bacterial challenge. The cytokines IL-1β, IL-4, IL-6, IL-10, IL-17A, IL-17F, IL-21, IL-22, IL23, IL-25, IL-31, IL-33, IFN-γ, sCD40L and TNF-α are related to Th17 response and play an important role in the pathogenesis of both diseases. Therefore, there is a need to evaluate the coexistence of these cytokines. The aim of this study was to analyze the levels of IL1-β, IL-4, IL-6, IL10, IL-17A, IL-17F, IL-21, IL-22, IL-23, IL-25, IL-31, IL-33, IFN-γ, TNF-α and sCD40L in the gingival tissue (G) and compare to the levels of the paired intestinal mucosa (I) in patients having both chronic periodontitis and IBD. Twenty-two IBD patients with chronic periodontitis and IBD, mean age 40.0±14.5, were enrolled in the study. Patients were assessed using anamnesis and periodontal examination. Gingival and intestinal biopsies were collected and homogenized using a cell disruptor. Cytokine s expression was evaluated through multiplex technology and then compensated by weight of samples. Mann-Whitney, Wilcoxon and Spearman correlation tests were used to analyze the data. Significant higher levels of gingival IL-23 (p=0.02) and IFN-γ (p=0.01), and significant lower levels of IL-31(p=0.02) and TNF-α (p=0.01) were found when compared to intestinal mucosa. Significant moderate correlations between gingival and intestinal tissue were noted to IL-6 (G) with IL-23(I) in a positive way and in a negative way to IL-23 (G) with IL-1β (I), IL-10 (I), IL-17A (I) and IFN-γ (I). We conclude that IL -23 and IFN- γ are significantly increased in the gingival tissue, when compared to the intestinal tissue, suggesting an important role of these cytokines in the manifestation of periodontitis in patients with IBD. The presence of significantly moderate correlation between IL -23 gingival with various cytokines in the intestinal tissue suggest the importance of IL -23 in interrelation with periodontitis and inflammatory bowel disease.