Influência do limite e do calibre apical após a instrumentação mecanizada com instrumentos reciprocantes na extrusão bacteriana apical

• Autor(a) principal: Teixeira, João Marcelo da Silva
• Data de Publicação: 2014
• Tipo de documento: Dissertação
• Idioma: por
• Título da fonte: Biblioteca Digital de Teses e Dissertações da UERJ
• Texto Completo: http://www.bdtd.uerj.br/handle/1/14165
• topic: Endodontics; Extrusion; Bacteria; Reciprocating; Files; Endodontia; Extrusão; Bacteriana; Instrumentação; Reciprocante; CNPQ::CIENCIAS DA SAUDE::ODONTOLOGIA

The aim of this work was to evaluate ex vivo the apical bacterial extrusion after instrumentation with a single instrument reciprocating system and the influence of differents working lengths and apical diameters on it. Sixty-four premolar roots were used. Endodontic access cavities were prepared and root canals were contaminated with an Enterococcus faecalis suspension and incubated for thirty days to promote bacterial biofilm growth. The contaminated teeth were divided into four groups with 15 specimens each. The files used and lengths from each group were respectively R25 at 0mm from foramen, R25 1mm shorter, R40 at 0mm from foramen and R40 1mm shorter. Positive and negative bacterial growth control groups were done. Bacterial extruded from the apical foramen during instrumentation were collected into vials containing 0.9% NaCl. The microbiological samples were taken from the vials and incubated in BHI agar medium during 24h. Bacterial growth was counted and the results were given by colony-forming units (CFU). These data were analyzed by Kruskal Wallis H-test. After data analysis, no significant difference was found in the number of CFU among the four groups (p > 0.05). Based on the results evaluation and within the limitation of this study, it was possible to conclude that regardless of the working length and foraminal enlargement will be similar amount of bacterial extrusion.