Flow cytometric screening for anti-leishmanials in a human macrophage cell line

Detalhes bibliográficos
Autor(a) principal: Mehta, Sanjay R.
Data de Publicação: 2010
Outros Autores: Xing, Quan Zhang, Badaró, Roberto José da Silva, Spina, Celsa, Day, John, Kwang, Poo Chang, Schooley, Robert T.
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da UFBA
Texto Completo: http://www.repositorio.ufba.br/ri/handle/ri/12109
Resumo: Texto completo: acesso restrito. p.617–620
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spelling Mehta, Sanjay R.Xing, Quan ZhangBadaró, Roberto José da SilvaSpina, CelsaDay, JohnKwang, Poo ChangSchooley, Robert T.Mehta, Sanjay R.Xing, Quan ZhangBadaró, Roberto José da SilvaSpina, CelsaDay, JohnKwang, Poo ChangSchooley, Robert T.2013-07-04T16:41:13Z20100014-4894http://www.repositorio.ufba.br/ri/handle/ri/12109v. 126, n. 4Texto completo: acesso restrito. p.617–620High-throughput drug screening methods against the intracellular stage of Leishmania have been facilitated by the development of in vitro models of infection. The use of cell lines rather than primary cells facilitates these methods. Peripheral blood mononuclear cell (PBMC) derived macrophages and THP-1 cells were infected with stationary phase egfp transfected Leishmania amazonensis parasites and then treated with anti-leishmanial compounds. Drug activity was measured using a flow cytometric approach, and toxicity was assessed using either the MTT assay or trypan blue dye exclusion. Calculated EC50’s for amphotericin B, sodium stibogluconate, and miltefosine were 0.1445 ± 0.0005 μg/ml, 0.1203 ± 0.018 mg/ml, and 26.71 μM using THP-1 cells, and 0.179 ± 0.035 μg/ml, 0.1948 ± 0.0364 mg/ml, and 13.77 ± 10.74 μM using PBMC derived macrophages, respectively. We conclude that a flow cytometric approach using egfp transfected Leishmania species can be used to evaluate anti-leishmanial compounds against the amastigote stage of the parasite in THP-1 cells with excellent concordance to human PBMC derived macrophages.Submitted by Suelen Reis (suziy.ellen@gmail.com) on 2013-07-03T14:47:56Z No. of bitstreams: 1 1-s2.0-S001448941000202X-main.pdf: 323382 bytes, checksum: 9edf6e6ac65090c1358eb67929c4e87e (MD5)Approved for entry into archive by NELIJANE MENEZES(rubi2276@gmail.com) on 2013-07-04T16:41:13Z (GMT) No. of bitstreams: 1 1-s2.0-S001448941000202X-main.pdf: 323382 bytes, checksum: 9edf6e6ac65090c1358eb67929c4e87e (MD5)Made available in DSpace on 2013-07-04T16:41:13Z (GMT). No. of bitstreams: 1 1-s2.0-S001448941000202X-main.pdf: 323382 bytes, checksum: 9edf6e6ac65090c1358eb67929c4e87e (MD5) Previous issue date: 2010-12http://dx.doi.org/10.1016/j.exppara.2010.06.007reponame:Repositório Institucional da UFBAinstname:Universidade Federal da Bahia (UFBA)instacron:UFBALeishmania amazonensisParasiteAmastigoteDrug screeningTHP-1Flow cytometryFlow cytometric screening for anti-leishmanials in a human macrophage cell lineExperimental Parasitologyinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article10000-01-01enginfo:eu-repo/semantics/openAccessORIGINAL1-s2.0-S001448941000202X-main.pdf1-s2.0-S001448941000202X-main.pdfapplication/pdf323382https://repositorio.ufba.br/bitstream/ri/12109/1/1-s2.0-S001448941000202X-main.pdf9edf6e6ac65090c1358eb67929c4e87eMD51LICENSElicense.txtlicense.txttext/plain1762https://repositorio.ufba.br/bitstream/ri/12109/2/license.txt1b89a9a0548218172d7c829f87a0eab9MD52TEXT1-s2.0-S001448941000202X-main.pdf.txt1-s2.0-S001448941000202X-main.pdf.txtExtracted texttext/plain19560https://repositorio.ufba.br/bitstream/ri/12109/3/1-s2.0-S001448941000202X-main.pdf.txt7d86745c061e123d599e3b5174a09b5dMD53ri/121092022-10-21 19:34:04.576oai:repositorio.ufba.br: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Repositório InstitucionalPUBhttp://192.188.11.11:8080/oai/requestopendoar:19322022-10-21T22:34:04Repositório Institucional da UFBA - Universidade Federal da Bahia (UFBA)false
dc.title.pt_BR.fl_str_mv Flow cytometric screening for anti-leishmanials in a human macrophage cell line
dc.title.alternative.pt_BR.fl_str_mv Experimental Parasitology
title Flow cytometric screening for anti-leishmanials in a human macrophage cell line
spellingShingle Flow cytometric screening for anti-leishmanials in a human macrophage cell line
Mehta, Sanjay R.
Leishmania amazonensis
Parasite
Amastigote
Drug screening
THP-1
Flow cytometry
title_short Flow cytometric screening for anti-leishmanials in a human macrophage cell line
title_full Flow cytometric screening for anti-leishmanials in a human macrophage cell line
title_fullStr Flow cytometric screening for anti-leishmanials in a human macrophage cell line
title_full_unstemmed Flow cytometric screening for anti-leishmanials in a human macrophage cell line
title_sort Flow cytometric screening for anti-leishmanials in a human macrophage cell line
author Mehta, Sanjay R.
author_facet Mehta, Sanjay R.
Xing, Quan Zhang
Badaró, Roberto José da Silva
Spina, Celsa
Day, John
Kwang, Poo Chang
Schooley, Robert T.
author_role author
author2 Xing, Quan Zhang
Badaró, Roberto José da Silva
Spina, Celsa
Day, John
Kwang, Poo Chang
Schooley, Robert T.
author2_role author
author
author
author
author
author
dc.contributor.author.fl_str_mv Mehta, Sanjay R.
Xing, Quan Zhang
Badaró, Roberto José da Silva
Spina, Celsa
Day, John
Kwang, Poo Chang
Schooley, Robert T.
Mehta, Sanjay R.
Xing, Quan Zhang
Badaró, Roberto José da Silva
Spina, Celsa
Day, John
Kwang, Poo Chang
Schooley, Robert T.
dc.subject.por.fl_str_mv Leishmania amazonensis
Parasite
Amastigote
Drug screening
THP-1
Flow cytometry
topic Leishmania amazonensis
Parasite
Amastigote
Drug screening
THP-1
Flow cytometry
description Texto completo: acesso restrito. p.617–620
publishDate 2010
dc.date.issued.fl_str_mv 2010
dc.date.accessioned.fl_str_mv 2013-07-04T16:41:13Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://www.repositorio.ufba.br/ri/handle/ri/12109
dc.identifier.issn.none.fl_str_mv 0014-4894
dc.identifier.number.pt_BR.fl_str_mv v. 126, n. 4
identifier_str_mv 0014-4894
v. 126, n. 4
url http://www.repositorio.ufba.br/ri/handle/ri/12109
dc.language.iso.fl_str_mv eng
language eng
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.source.pt_BR.fl_str_mv http://dx.doi.org/10.1016/j.exppara.2010.06.007
dc.source.none.fl_str_mv reponame:Repositório Institucional da UFBA
instname:Universidade Federal da Bahia (UFBA)
instacron:UFBA
instname_str Universidade Federal da Bahia (UFBA)
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reponame_str Repositório Institucional da UFBA
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